Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 9,907 A→C 16.2% intergenic (+14/+21) mog → / ← satP molybdochelatase incorporating molybdenum into molybdopterin/succinate‑acetate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009139,9070AC16.2% 206.7 / 43.0 93intergenic (+14/+21)mog/satPmolybdochelatase incorporating molybdenum into molybdopterin/succinate‑acetate transporter
Reads supporting (aligned to +/- strand):  ref base A (37/41);  new base C (8/7);  total (45/48)
Fisher's exact test for biased strand distribution p-value = 7.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.54e-02

TGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTG  >  NC_000913/9808‑10004
                                                                                                   |                                                                                                 
tGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGAGGGAAGAGGGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGa                                                                                                   >  2:564602/1‑100 (MQ=255)
 gCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAg                                                                                                  >  2:2075559/1‑100 (MQ=255)
 gCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAg                                                                                                  >  1:1327616/1‑100 (MQ=255)
  cTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGc                                                                                                 >  1:2264163/1‑100 (MQ=255)
    ggAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGCGCGCAAGACGGGCCGTTAGCGAATAAAAAAATCCCACCGAGc                                                                                                 >  1:569457/1‑98 (MQ=255)
    ggAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCgg                                                                                               >  2:1019117/1‑100 (MQ=255)
    ggAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCgg                                                                                               >  1:270735/1‑100 (MQ=255)
      aaGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCgggg                                                                                             <  1:2089844/100‑1 (MQ=255)
         ggCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGa                                                                                          <  1:1441715/100‑1 (MQ=255)
         ggCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGa                                                                                          <  1:10218/100‑1 (MQ=255)
          gCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGCTCGGGGGGAt                                                                                         <  1:796217/100‑1 (MQ=255)
           ccATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGAtc                                                                                        <  1:1742345/100‑1 (MQ=255)
            cATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGCTCGGGGGGAttt                                                                                       <  1:1977688/100‑3 (MQ=255)
               aCGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGCTCGGGGGGAtttttt                                                                                    <  2:99978/100‑6 (MQ=255)
                cGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGCTCGGGGGGAttttttt                                                                                   <  1:47535/100‑7 (MQ=39)
                 gTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCaaaa                                                                                  >  1:1878510/1‑100 (MQ=255)
                 ccgatctcGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCaaaa                                                                                  <  2:2338555/93‑1 (MQ=255)
                  ttGAAACGGCCGCGCAAGTGGTTGCACCATTCAGACCGAAGAGTGCAAGACGTGACGTTAGCGAATAAAAAAATCCCCCCGCTCGGGCGGAtttttttat                                                                                 <  2:2159702/100‑9 (MQ=255)
                  ttGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGCTCGGGGGGAtttttttat                                                                                 <  1:2141169/100‑9 (MQ=38)
                   tGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACa                                                                                >  2:700041/1‑100 (MQ=255)
                    gAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACaa                                                                               >  2:540793/1‑100 (MQ=255)
                    gAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACa                                                                                <  2:94793/99‑1 (MQ=255)
                     aaaCGGCACCGGAAGTGGTGGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAAt                                                                              <  1:1420142/100‑1 (MQ=255)
                      aaCGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAAtt                                                                             <  1:2123291/100‑1 (MQ=255)
                        cGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCaaaaagatcgg                                                                           >  1:2338576/1‑93 (MQ=255)
                        cGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAg                                                                           >  2:1934433/1‑100 (MQ=255)
                          gCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTg                                                                         <  2:1230526/100‑1 (MQ=255)
                             ccGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTggg                                                                       <  2:456893/99‑1 (MQ=255)
                             ccGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGa                                                                      >  2:1309215/1‑100 (MQ=255)
                                 ttccgatctcagcaTTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCa                                                                  <  1:1138015/91‑1 (MQ=255)
                                 aaGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCa                                                                  <  1:2308586/100‑1 (MQ=255)
                                  aGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAACTCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCaa                                                                 <  2:906143/100‑2 (MQ=255)
                                  aGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCAc                                                                 <  1:1380730/100‑1 (MQ=255)
                                  aGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCAc                                                                 >  2:2103792/1‑100 (MQ=255)
                                   gTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCAcc                                                                <  1:2075577/100‑1 (MQ=255)
                                    tGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTCGCGAATAAAAAAATCCCCCCGACCGGGGGGATCTCAAAACAATTAGTGGGATTCACCa                                                               >  2:1067910/1‑100 (MQ=255)
                                     ggTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAACTCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCaa                                                              >  2:760136/1‑100 (MQ=255)
                                      gTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAAt                                                             >  1:414832/1‑100 (MQ=255)
                                        tGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCg                                                           <  1:1526104/100‑1 (MQ=255)
                                        tGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCg                                                           <  1:1080245/100‑1 (MQ=255)
                                        tGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCg                                                           >  2:1754570/1‑100 (MQ=255)
                                         gcagcaTTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCgg                                                          >  2:220078/1‑100 (MQ=255)
                                         gcagcaTTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCgg                                                          >  2:1824136/1‑100 (MQ=255)
                                          cagcaTTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCaagatcggaa                                                         >  2:1138010/1‑91 (MQ=255)
                                          cagcaTTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGc                                                         <  2:1756900/100‑1 (MQ=255)
                                            gcaTTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAACAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGc                                                         <  1:1060919/98‑1 (MQ=255)
                                             caTTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGa                                                      >  1:1363646/1‑100 (MQ=255)
                                               ttCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAAc                                                    <  1:2256363/100‑1 (MQ=255)
                                               ttCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAAc                                                    <  1:352191/100‑1 (MQ=255)
                                                 cAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCACCCGAGCGGGGGAATCTCAAAACAATGAGTGGGATTCACCAATCGGCAGAACgg                                                  >  2:428747/1‑100 (MQ=255)
                                                   gACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTg                                                >  1:1424504/1‑100 (MQ=255)
                                                   gACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTg                                                <  1:19668/100‑1 (MQ=255)
                                                   gACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTg                                                >  1:2367899/1‑100 (MQ=255)
                                                       gAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGAc                                            <  1:537455/100‑1 (MQ=255)
                                                       gAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAAGCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGAc                                            <  2:305869/100‑1 (MQ=255)
                                                         agagTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCa                                          >  1:2162164/1‑100 (MQ=255)
                                                            gTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAAc                                       >  2:126061/1‑100 (MQ=255)
                                                            gTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAAc                                       >  1:1088833/1‑100 (MQ=255)
                                                             tGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACt                                      <  1:1468564/100‑1 (MQ=255)
                                                             tGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACt                                      <  2:1257724/100‑1 (MQ=255)
                                                              gCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTg                                     >  2:455456/1‑100 (MQ=255)
                                                              gCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTg                                     <  1:2375880/100‑1 (MQ=255)
                                                                aaGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCt                                   <  2:776257/100‑1 (MQ=255)
                                                                aaGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCt                                   <  1:220079/100‑1 (MQ=255)
                                                                 aGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTc                                  >  1:589900/1‑100 (MQ=255)
                                                                    cgcgACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGtt                               >  1:911259/1‑100 (MQ=255)
                                                                     gcgACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTc                              <  2:1777586/100‑1 (MQ=255)
                                                                      cgACGTAAGCGATGAAAAAAATACCCCCGAGCGGGTGGATCTCTTAACAATTGGTGGCATTCACCAATCGGCAGAACTGTGCGACCAATCTGCTCGTTc                              <  2:907477/99‑1 (MQ=255)
                                                                       gACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCa                             <  2:375309/99‑1 (MQ=255)
                                                                          gTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTAc                         <  1:2051775/100‑1 (MQ=255)
                                                                          gTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTAc                         <  1:1576755/100‑1 (MQ=255)
                                                                            tAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACtt                       >  2:2193823/1‑100 (MQ=255)
                                                                               cGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCAc                    <  2:1837195/100‑1 (MQ=255)
                                                                                ggaTAAGAAAATCCCCCCGAGCGGGGGGATCTAAAAAAAATTAATGGGATTCAATAATCGGCAGAACGGTGCGAACAAACTGCTCGTTCAGTAc                         >  2:370079/3‑94 (MQ=255)
                                                                                    cttgagaTCCCCCCGCTCGGGGGGATTTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATc               <  1:198893/94‑1 (MQ=38)
                                                                                    aaaaaaaTCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATc               <  2:619493/100‑1 (MQ=255)
                                                                                     ttgagaTCCCCCCGCTCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCg              >  1:1057208/6‑100 (MQ=255)
                                                                                     aaaaaaTCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCg              >  2:1008659/1‑100 (MQ=255)
                                                                                      tgagaTCCCCCCGCTCGGGGGGATTTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATcag             >  2:198892/5‑98 (MQ=38)
                                                                                       gagaTCCCCCCGCTCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGcc            >  1:1619597/4‑100 (MQ=255)
                                                                                        agaTCCCCCCGCTCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCa           >  1:1870625/3‑100 (MQ=255)
                                                                                         gaTCCCCCCTAGCGGGGGGATCTCAAAGCAATTAGTGGGTTTCACCAATCGGCCGAACTGTGCGATCAAACTGCTCGTTCAGTACTTCAccc                  <  2:1577972/91‑1 (MQ=255)
                                                                                         gaTCCCCCCGCTCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCag          >  1:55325/2‑100 (MQ=255)
                                                                                         gaTCCCCCCGCTCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCag          >  2:710486/2‑100 (MQ=255)
                                                                                         gaTCCCCCCGCTCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCa           >  2:2257456/2‑99 (MQ=255)
                                                                                           tCCCCCCGCTCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCagat        >  1:1141287/1‑100 (MQ=255)
                                                                                            ccccccGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCagata       <  1:616550/100‑1 (MQ=255)
                                                                                            ccccccGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCagata       >  1:2055723/1‑100 (MQ=255)
                                                                                             cccccGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCagatag      >  1:2069144/1‑100 (MQ=255)
                                                                                              ccccGAGCGGGGGGATCTCAAAAAAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCCAACTGCTAGTTCAGTACTTCACCCATCGCCacataga     >  2:2215560/1‑100 (MQ=255)
                                                                                                 cGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGAtt   <  2:54353/99‑1 (MQ=255)
                                                                                                 cGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGAtt   <  1:1285763/99‑1 (MQ=255)
                                                                                                 cGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTg  <  2:1402411/100‑1 (MQ=255)
                                                                                                 cGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTg  >  2:1537786/1‑100 (MQ=255)
                                                                                                   |                                                                                                 
TGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTG  >  NC_000913/9808‑10004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: