Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,312,089 A→G 17.2% intergenic (‑47/+12) alsB ← / ← alsR D‑allose ABC transporter periplasmic binding protein/d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,312,0890AG17.2% 247.0 / 38.6 108intergenic (‑47/+12)alsB/alsRD‑allose ABC transporter periplasmic binding protein/d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR
Reads supporting (aligned to +/- strand):  ref base A (42/47);  new base G (12/7);  total (54/54)
Fisher's exact test for biased strand distribution p-value = 3.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01

Only 100 of 114 total aligned reads displayed.

GACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCG  >  NC_000913/4311990‑4312188
                                                                                                   |                                                                                                   
gACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGaa                                                                                                     >  1:1090134/1‑100 (MQ=255)
   aacaTTAAGCCCACGAGTGTGCCGCTGAAATATTACAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTa                                                                                                  <  1:1293841/100‑1 (MQ=255)
     caTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTAcc                                                                                                <  1:1174408/100‑1 (MQ=38)
     caTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTAcc                                                                                                <  1:2197516/100‑1 (MQ=38)
     caTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTAcc                                                                                                >  1:2199780/1‑100 (MQ=255)
      aTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTATGGGGACAGGTAACTCCGCGATGGCGGAATTACCt                                                                                               >  2:1257814/1‑100 (MQ=255)
       ttAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtg                                                                                              <  2:559020/100‑2 (MQ=38)
       ttAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtg                                                                                              <  2:2118873/100‑2 (MQ=38)
       ttAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCtt                                                                                              >  2:922470/1‑100 (MQ=255)
        tAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtgt                                                                                             <  1:2095995/100‑3 (MQ=38)
             ccACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTc                                                                                          <  2:2198333/98‑1 (MQ=255)
             ccACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCtt                                                                                        >  1:2196662/1‑100 (MQ=255)
             ccACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTATCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCtt                                                                                        >  2:1038165/1‑100 (MQ=255)
              cACGAGTGTGGCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGTCATGTAATTCCGTGATGGCGGAATTACCttttac                                                                                          <  2:239589/97‑3 (MQ=255)
                  aGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTAtt                                                                                     <  2:240125/98‑1 (MQ=255)
                     gtgCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgc                                                                                >  2:1931234/1‑100 (MQ=255)
                       gCCGCTGAAATATTTCAGATATTTATTCATAATAATGATCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgcgc                                                                              >  2:1275841/1‑100 (MQ=255)
                        gcGCTGAAGTATTTCAGATATTTATTCATAATAATGTTAGTACGGTTAGGGGACAAGTAACTCCGCGTTGGCGGAGTTAAGTTATGATTATTTCAgcgcg                                                                             >  2:564088/2‑100 (MQ=255)
                        ccGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgcgcg                                                                             <  1:556143/100‑1 (MQ=255)
                         cGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgcgcgc                                                                            >  1:1205421/1‑100 (MQ=255)
                             gAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTgg                                                                        >  1:1477255/1‑100 (MQ=255)
                             gAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTgg                                                                        >  1:2376182/1‑100 (MQ=255)
                               aaTATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTGCCTTTTTCTTATTTCAGCGCGCCTGGTg                                                                      <  2:1720402/100‑1 (MQ=255)
                               aaTATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTg                                                                      <  2:522284/100‑1 (MQ=255)
                               aaTATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTg                                                                      <  2:1779495/100‑1 (MQ=255)
                               aaTATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTg                                                                      <  1:1324563/100‑1 (MQ=255)
                                    ttCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGagaag                                                                 >  2:1629002/1‑100 (MQ=255)
                                       aGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGTTAACTCCGCGATGGCGGCATTACCTTTTTCTTATTTCCGCGCGCCTGGTGagaa                                                                  >  2:1507109/1‑96 (MQ=255)
                                        gATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAAt                                                             <  2:1615285/100‑1 (MQ=255)
                                         atatTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATc                                                            <  1:43246/100‑1 (MQ=255)
                                          tatttattCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCGGCGCGCCTGGTGAGAAGAAATca                                                           <  1:79217/100‑1 (MQ=255)
                                          tatttattCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATca                                                           >  2:1598103/1‑100 (MQ=255)
                                           atttattCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATcaa                                                          <  1:1877431/100‑1 (MQ=255)
                                           atttattCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATcaa                                                          <  2:754897/100‑1 (MQ=255)
                                                 tCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTg                                                    >  2:888643/1‑100 (MQ=255)
                                                   ataataATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGTGCCTGGTGAGAAGAAATCAACAATTgcg                                                  >  2:754235/1‑100 (MQ=255)
                                                   ataataATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTgcg                                                  >  1:1293594/1‑100 (MQ=255)
                                                    taataaTGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTgcgc                                                 >  1:1223055/1‑100 (MQ=255)
                                                    taataaTGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTGCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTgcgc                                                 >  2:1230614/1‑100 (MQ=255)
                                                      ataatGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCg                                               <  1:950224/100‑1 (MQ=255)
                                                       taatGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCgg                                              >  2:432899/1‑100 (MQ=255)
                                                           gTTCTCACGGTAAGGGGACAGGTAACGCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGtttt                                          <  2:1865026/100‑1 (MQ=255)
                                                            ttCTCACGGTAAGGGGACAGGTAACTACGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGttttt                                         <  1:1660903/100‑1 (MQ=255)
                                                             tctcACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGtttttt                                        >  1:157621/1‑100 (MQ=255)
                                                              ctcACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTg                                       <  1:305604/100‑1 (MQ=255)
                                                              ctcACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTg                                       >  1:1518187/1‑100 (MQ=255)
                                                                 aCGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCat                                    >  2:144003/1‑100 (MQ=255)
                                                                 aCGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCat                                    <  1:1260928/100‑1 (MQ=255)
                                                                 aCGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCat                                    <  1:1275848/100‑1 (MQ=255)
                                                                   ggTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatat                                  >  1:1180402/1‑100 (MQ=255)
                                                                   ggTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatat                                  <  1:1702098/100‑1 (MQ=255)
                                                                   ggTAAGCGGACAGGTAACTCCGCGATTGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTCGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatat                                  <  2:370070/100‑1 (MQ=255)
                                                                    gTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGTTTTTTTGCatatt                                 <  1:1620353/100‑1 (MQ=255)
                                                                    gTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatatt                                 <  1:2275896/100‑1 (MQ=255)
                                                                      aaGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaa                               >  2:563172/1‑100 (MQ=255)
                                                                      aaGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaa                               <  1:109458/100‑1 (MQ=255)
                                                                      aaGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaa                               <  2:1865493/100‑1 (MQ=255)
                                                                      aaGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaa                               <  1:1909660/100‑1 (MQ=255)
                                                                      aaGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaa                               <  2:1553391/100‑1 (MQ=255)
                                                                       aagaaaaaGGTAATTCCACGATCGAGGAGATACCTTTTTCTTATTTCAGCGCGCCTGGGGAGAAGAATTTATGAATTTAGCAGGTTTTTTGCatatt                                 >  2:468778/8‑97 (MQ=255)
                                                                        ggggACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaata                             <  2:1205414/100‑1 (MQ=255)
                                                                        ggagACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatatta                                <  2:2385505/94‑1 (MQ=255)
                                                                        agaaaaaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaata                             >  1:953140/7‑100 (MQ=25)
                                                                        agaaaaaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaata                             >  1:2314436/7‑100 (MQ=25)
                                                                        agaaaaaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCGGGTGAGAAGAAGTCAACAATTGCGCCGGTTTTTTGCatattaata                             >  2:2071530/7‑100 (MQ=25)
                                                                         gggACAGGTCACTCCGCGATGGGGGAATTACCGTGTTCGTAGGTCAGCACGCCTGGGGAGAAGAATTCAACAATTGCGCCTGTTGTGGGCatattaa                               >  2:195988/1‑97 (MQ=255)
                                                                          aaaaaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaatatt                           >  1:1102679/5‑100 (MQ=37)
                                                                            aaaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAg                         >  1:411355/3‑100 (MQ=38)
                                                                             aaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGc                        >  2:2102562/2‑100 (MQ=38)
                                                                             aaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGc                        >  2:1078982/2‑100 (MQ=38)
                                                                              aagTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCt                       >  1:1614308/3‑100 (MQ=38)
                                                                              aGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGACGAAATCAACAATTGCGCCGGTTTTTTTCATATTAATATTAg                         >  2:1588103/1‑98 (MQ=37)
                                                                              aGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCt                       >  2:457081/1‑100 (MQ=38)
                                                                               ggTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCtt                      >  1:365150/1‑100 (MQ=255)
                                                                               ggTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCtt                      >  1:987805/1‑100 (MQ=255)
                                                                                atAACTCCGCGATGGCGGAATTACCCTTTTCTTATTTCAACACGCCTAGTAAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATAGTAGc                        <  2:2232952/96‑1 (MQ=255)
                                                                                 tAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGt                    >  1:516534/1‑100 (MQ=255)
                                                                                   tcTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTc                  <  1:1603409/99‑1 (MQ=255)
                                                                                   attCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTc                  <  2:1614296/98‑1 (MQ=255)
                                                                                   aCTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTc                  <  1:914456/100‑1 (MQ=255)
                                                                                    cTCCGCGATGGCGGAATTACCTTTTTTTTTTTTTTTCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTc                  >  2:1603397/1‑99 (MQ=255)
                                                                                    cTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCa                 >  1:1514619/1‑100 (MQ=255)
                                                                                      ccGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAAt               <  1:1038169/100‑1 (MQ=255)
                                                                                      ccGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAAt               >  1:1251156/1‑100 (MQ=255)
                                                                                      ccGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAAt               >  2:689223/1‑100 (MQ=255)
                                                                                      ccGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAAt               >  2:1420958/1‑100 (MQ=255)
                                                                                       cgcgATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATg              <  2:2196642/100‑1 (MQ=255)
                                                                                       cgcgATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATg              <  2:122375/100‑1 (MQ=255)
                                                                                       cgcgATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATg              <  1:25265/100‑1 (MQ=255)
                                                                                           aTGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGa          <  2:200444/100‑1 (MQ=255)
                                                                                              gCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCt       >  1:1442044/1‑100 (MQ=255)
                                                                                               cGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTg      >  1:960131/1‑100 (MQ=255)
                                                                                                ggAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTGTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTgg     <  2:1737055/100‑1 (MQ=255)
                                                                                                 gAATTACCTTTTTCTTATTTCAGCGCGCCTTGTTAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTgg     <  2:541276/99‑1 (MQ=255)
                                                                                                 gAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTggg    <  1:1629014/100‑1 (MQ=255)
                                                                                                  aaTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGc   <  2:439435/100‑1 (MQ=255)
                                                                                                  aaTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGc   <  2:1150096/100‑1 (MQ=255)
                                                                                                  aaTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGc   <  2:875708/100‑1 (MQ=255)
                                                                                                   aTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCg  >  1:1332024/1‑100 (MQ=255)
                                                                                                   aTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCg  <  2:85458/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCG  >  NC_000913/4311990‑4312188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: