Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,376,280 G→A 17.3% intergenic (+15/‑37) efp → / → ecnA polyproline‑specific translation elongation factor EF‑P/entericidin A membrane lipoprotein, antidote entericidin B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,376,2800GA17.3% 245.2 / 40.1 104intergenic (+15/‑37)efp/ecnApolyproline‑specific translation elongation factor EF‑P/entericidin A membrane lipoprotein, antidote entericidin B
Reads supporting (aligned to +/- strand):  ref base G (36/50);  new base A (10/8);  total (46/58)
Fisher's exact test for biased strand distribution p-value = 3.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.39e-01

Only 100 of 111 total aligned reads displayed.

GGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACC  >  NC_000913/4376181‑4376379
                                                                                                   |                                                                                                   
ggTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCg                                                                                                     >  1:1769306/1‑100 (MQ=255)
 gTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCgg                                                                                                    >  2:875515/1‑100 (MQ=255)
 gTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCgg                                                                                                    <  2:397378/100‑1 (MQ=255)
  ttAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGc                                                                                                   <  1:329728/100‑1 (MQ=255)
   tAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGcc                                                                                                  >  1:1154185/1‑100 (MQ=255)
      aGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGc                                                                                               <  1:2144053/100‑1 (MQ=255)
      aGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGc                                                                                               <  2:1021077/100‑1 (MQ=255)
       gTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCa                                                                                              >  1:2063737/1‑100 (MQ=255)
          ccGCTGTTTGTACAAATCGGCGAAGTCATGAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGc                                                                                           <  2:1994569/100‑1 (MQ=255)
          ccGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGc                                                                                           >  2:1011755/1‑100 (MQ=255)
          ccGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGc                                                                                           <  1:824990/100‑1 (MQ=255)
          ccGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGc                                                                                           <  1:1750655/100‑1 (MQ=255)
           cGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCt                                                                                          <  2:1947903/100‑1 (MQ=255)
            gCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTg                                                                                         <  2:907173/100‑1 (MQ=255)
             cTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGc                                                                                        <  1:615072/100‑1 (MQ=255)
             cTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGc                                                                                        <  1:2115117/100‑1 (MQ=255)
              tGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCa                                                                                       <  1:2368179/100‑1 (MQ=255)
              tGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCa                                                                                       <  1:1865726/100‑1 (MQ=255)
              tGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTATCTCGCGTAAAGAAATGCGGTTGTGGTGCGGCCTGCAGGCTGc                                                                                        >  2:816601/1‑99 (MQ=255)
               gTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCAc                                                                                      <  2:434195/100‑1 (MQ=255)
               gTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCAc                                                                                      >  1:972347/1‑100 (MQ=255)
               gTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCAc                                                                                      <  1:82667/100‑1 (MQ=255)
                tttGTACAAATCGGCGAAGTCATCAAGGTGGGTACCCGTTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCAcc                                                                                     <  2:1485936/100‑1 (MQ=255)
                tttGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCAcc                                                                                     <  2:1952950/100‑1 (MQ=255)
                tttGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCAcc                                                                                     <  1:106825/100‑1 (MQ=255)
                tttGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCAcc                                                                                     <  1:488311/100‑1 (MQ=255)
                 ttGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTCAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCAcc                                                                                     <  2:1276942/99‑1 (MQ=255)
                  tGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCAt                                                                                   >  1:2078604/1‑100 (MQ=255)
                    tACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCa                                                                                 <  2:2178410/100‑1 (MQ=255)
                     aCAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCAc                                                                                >  1:867288/1‑100 (MQ=255)
                     aCAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCAc                                                                                <  2:1613772/100‑1 (MQ=255)
                     aCAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCAc                                                                                <  1:875517/100‑1 (MQ=255)
                     aCAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCAc                                                                                <  1:1818692/100‑1 (MQ=255)
                      cAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACt                                                                               >  1:1259148/1‑100 (MQ=255)
                      cAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCACCacaacc                                                                               <  1:1161599/100‑6 (MQ=38)
                        aaTCGGCGAAGTCATCAGAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTa                                                                             <  2:2033984/100‑1 (MQ=255)
                        aaTCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCAc                                                                                >  2:408213/1‑97 (MQ=255)
                         aTCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTAt                                                                            <  2:1115269/100‑1 (MQ=255)
                         aTCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTAt                                                                            <  2:847321/100‑1 (MQ=255)
                           cGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTc                                                                          >  2:1489944/1‑100 (MQ=255)
                           cGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTc                                                                          <  2:1789759/100‑1 (MQ=255)
                            ggCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCa                                                                         >  1:404749/1‑100 (MQ=255)
                            ggCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCa                                                                         <  2:1836908/100‑1 (MQ=255)
                             gCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAg                                                                        >  1:1759656/1‑100 (MQ=255)
                              cGAAGTCATCACAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGACGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAgg                                                                       >  2:2072255/1‑100 (MQ=255)
                              cGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAgg                                                                       <  1:1749888/100‑1 (MQ=255)
                                aaGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTc                                                                     <  2:570086/100‑1 (MQ=255)
                                 aGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGTAGTAATGCGTTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGtaa                                                                    >  2:155058/1‑98 (MQ=255)
                                    catcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCagag                                                                 >  2:283343/1‑100 (MQ=255)
                                    catcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCagag                                                                 >  1:2092516/1‑100 (MQ=255)
                                    catcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCagag                                                                 >  1:1940230/1‑100 (MQ=255)
                                     atcaAAGTGGATACCCGCGCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAgaga                                                                <  2:2327711/100‑1 (MQ=255)
                                      tcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGGCAGAgat                                                               >  1:1209002/1‑100 (MQ=255)
                                          aGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCGGCAGGCTGCACCATCACTTATTCAGGTCAGAGatgatg                                                           <  2:1465550/100‑1 (MQ=255)
                                                  cccGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCt                                                   >  1:1974815/1‑100 (MQ=255)
                                                  cccGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCt                                                   >  1:502313/1‑100 (MQ=255)
                                                   ccGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCtt                                                  <  1:2304940/100‑1 (MQ=255)
                                                       tctGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCg                                              <  2:1898500/100‑1 (MQ=255)
                                                        ctGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGt                                             <  2:471536/100‑1 (MQ=255)
                                                          ggTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTc                                           >  1:1100556/1‑100 (MQ=255)
                                                          ggTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTc                                           >  2:1618439/1‑100 (MQ=255)
                                                                tACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGttt                                     <  1:423539/100‑1 (MQ=255)
                                                                tACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGttt                                     <  1:480035/100‑1 (MQ=255)
                                                                  cGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCCCTTATTCAGGTCAGCGATGATGAAACGCCTTATCGTTCTTGtttt                                    >  2:2287873/1‑99 (MQ=255)
                                                                  cGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttg                                   <  1:138391/100‑1 (MQ=255)
                                                                  cGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttg                                   >  2:138390/1‑100 (MQ=255)
                                                                  cGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttg                                   <  1:2079638/100‑1 (MQ=255)
                                                                    tctttcGCGTGAAGTAATGCGGTTGTGGTGCGGCATGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgct                                 <  2:102308/100‑1 (MQ=255)
                                                                    tctctcGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgct                                 <  2:1011622/100‑1 (MQ=255)
                                                                      tctcGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgcttg                               <  2:730136/100‑1 (MQ=255)
                                                                       ctcGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgcttgc                              >  2:1751642/1‑100 (MQ=255)
                                                                         cgcgTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCa                            >  1:312390/1‑100 (MQ=255)
                                                                          gcgTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAg                           <  2:1739086/100‑1 (MQ=255)
                                                                          gcgTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAg                           <  1:353149/100‑1 (MQ=255)
                                                                               aaGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACgc                      <  2:1957184/100‑1 (MQ=255)
                                                                                  tAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACgctgc                   <  1:1330654/100‑1 (MQ=255)
                                                                                  tAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACgctg                    >  2:1265180/1‑99 (MQ=255)
                                                                                   aaTGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACgctgct                  >  2:381138/1‑100 (MQ=255)
                                                                                    aTGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTc                 >  1:16827/1‑100 (MQ=255)
                                                                                       cGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACg              >  1:2222831/1‑100 (MQ=255)
                                                                                        ggTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACgg             <  1:1754419/100‑1 (MQ=255)
                                                                                        agtgatggtgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACgg             >  2:607107/6‑100 (MQ=38)
                                                                                         gtgatggtgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACggg            >  1:378436/5‑100 (MQ=39)
                                                                                         gtgatggtgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACggg            >  2:1575770/5‑100 (MQ=39)
                                                                                          ttGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGc           <  2:502311/100‑1 (MQ=255)
                                                                                          tgatggtgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGc           <  1:1706781/97‑1 (MQ=39)
                                                                                           gatggtgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGca          >  2:1706768/3‑99 (MQ=39)
                                                                                           gatggtgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCt          >  2:1221155/3‑100 (MQ=255)
                                                                                           gatggtgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCt          >  1:145251/3‑100 (MQ=255)
                                                                                            gtggtgCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTg         <  1:207822/100‑1 (MQ=255)
                                                                                            atggtgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTg         >  1:488362/2‑100 (MQ=255)
                                                                                            atggtgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTg         >  1:11492/2‑100 (MQ=255)
                                                                                              ggtgCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTa       <  2:2063719/100‑1 (MQ=255)
                                                                                              ggtgCGGCCGGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTa       <  2:2009889/100‑1 (MQ=255)
                                                                                               gtgCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTaa      >  2:1826883/1‑100 (MQ=255)
                                                                                               gtgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTaa      >  2:247326/1‑100 (MQ=255)
                                                                                                 gCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAaca    <  1:1202444/100‑1 (MQ=255)
                                                                                                   ggCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACAcc  >  1:395005/1‑100 (MQ=255)
                                                                                                   ggCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACAcc  <  1:1691041/100‑1 (MQ=255)
                                                                                                   ggCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACAcc  <  1:206960/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACC  >  NC_000913/4376181‑4376379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: