Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 481,225 T→G 17.5% intergenic (‑517/+29) tomB ← / ← acrB Hha toxicity attenuator; conjugation‑related protein/multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913481,2250TG17.5% 307.8 / 59.8 126intergenic (‑517/+29)tomB/acrBHha toxicity attenuator; conjugation‑related protein/multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base T (49/55);  new base G (18/4);  total (67/59)
Fisher's exact test for biased strand distribution p-value = 4.10e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 127 total aligned reads displayed.

TGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACC  >  NC_000913/481126‑481323
                                                                                                   |                                                                                                  
tGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCt                                                                                                    >  1:715409/1‑100 (MQ=255)
  aaTTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTa                                                                                                  >  2:217451/1‑100 (MQ=255)
      ttATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTAcgcg                                                                                              >  2:1423058/1‑100 (MQ=255)
      ttATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTAcgcg                                                                                              >  1:2192381/1‑100 (MQ=255)
      ttATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTAcgcg                                                                                              >  1:1851802/1‑100 (MQ=255)
        aTCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGc                                                                                            <  2:400815/100‑1 (MQ=255)
        aTCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTGCAATTACGCCTTAATGTTCTTAGGTTATGCATAAAAAAGGCCGCTTACGCGGc                                                                                            >  1:1036146/1‑100 (MQ=255)
         tCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGcc                                                                                           >  1:41135/1‑100 (MQ=255)
             tGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTANTCCTNAANGTTCNTAGNTTATGCATAAAAAAGGCCGCGTAAGCGGCCtttn                                                                                       <  1:1146038/100‑3 (MQ=255)
             tGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAg                                                                                       <  1:738517/100‑1 (MQ=255)
             tGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAg                                                                                       <  1:335414/100‑1 (MQ=255)
              ggTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGt                                                                                      <  1:1314298/100‑1 (MQ=255)
              ggTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCttttt                                                                                      <  1:1781507/100‑4 (MQ=255)
               gTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTg                                                                                     <  2:41134/100‑1 (MQ=255)
                 tAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGAt                                                                                   <  1:419000/100‑1 (MQ=255)
                  aaTACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGAtt                                                                                  <  1:1221684/100‑1 (MQ=255)
                  aaTACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGAt                                                                                   <  1:2063828/99‑1 (MQ=255)
                  aaTACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCttttttatg                                                                                  <  2:1918449/100‑8 (MQ=38)
                    tACTGGTTTTCGTATGAGATCCTGAGTTTGTGGTTCAATTACTCCTTTATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTac                                                                                <  2:817745/100‑1 (MQ=255)
                      cTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTacac                                                                              >  2:1913518/1‑100 (MQ=255)
                       tGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACg                                                                             <  1:1627653/100‑1 (MQ=255)
                        ggTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGt                                                                            <  1:2324926/100‑1 (MQ=255)
                         gTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGtt                                                                           <  2:904255/100‑1 (MQ=255)
                          ttttCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTg                                                                          >  1:734894/1‑100 (MQ=255)
                          ttttCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTg                                                                          <  2:2074376/100‑1 (MQ=255)
                           tttCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGt                                                                         >  2:273785/1‑100 (MQ=255)
                           tttCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGt                                                                         <  1:277119/100‑1 (MQ=255)
                           tttCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGt                                                                         >  1:283353/1‑100 (MQ=255)
                           tttCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGATGt                                                                         <  2:1665009/100‑1 (MQ=255)
                            ttCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTa                                                                        <  1:1440388/100‑1 (MQ=255)
                              cGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATc                                                                      <  2:289044/100‑1 (MQ=255)
                              cGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATc                                                                      <  1:1842289/100‑1 (MQ=255)
                                tATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCaa                                                                    >  1:2306/1‑100 (MQ=255)
                                    agaTCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAatgct                                                                >  2:1016120/1‑98 (MQ=255)
                                    agaTCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAatgat                                                                >  2:1211861/1‑100 (MQ=255)
                                       tCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATgatgat                                                             >  2:937473/1‑100 (MQ=255)
                                           gAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATc                                                            <  2:913890/97‑1 (MQ=255)
                                           gAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGAc                                                         <  2:2256681/100‑1 (MQ=255)
                                               tggtggTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTa                                                     >  1:1311765/1‑100 (MQ=255)
                                               tggtggTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATCATAGCCAgca                                                     <  2:1637692/100‑3 (MQ=255)
                                                ggtggtTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTAt                                                    >  1:88942/1‑100 (MQ=255)
                                                ggtggtTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTAt                                                    <  2:723093/100‑1 (MQ=255)
                                                 gtggtTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATg                                                   <  1:735809/100‑1 (MQ=255)
                                                   ggtTGAATGACTCCTTAATGTTGGTAGGTGATGCAGATGAAAGGCCGTTTACGCGGCCTTAGTGAGTAGAGGTTGTGACAATGATGATCGACAGTATGGc                                                 <  2:1656534/100‑1 (MQ=255)
                                                     ttCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCtg                                               <  2:124329/100‑1 (MQ=255)
                                                      tCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGCCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCtg                                               >  2:429599/1‑99 (MQ=255)
                                                       cAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCtgtg                                             >  2:4998/1‑100 (MQ=255)
                                                        aaTTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGc                                            <  2:1828793/100‑1 (MQ=255)
                                                         aTTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCt                                           >  1:1382983/1‑100 (MQ=255)
                                                          ttACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTc                                          <  2:2192361/100‑1 (MQ=255)
                                                          ttACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTc                                          <  2:1851788/100‑1 (MQ=255)
                                                          ttACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTc                                          <  1:1608258/100‑1 (MQ=255)
                                                           tACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCg                                         <  1:652344/100‑1 (MQ=255)
                                                            aCTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTc                                          <  2:766241/98‑1 (MQ=255)
                                                            aCTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGa                                        <  1:659098/100‑1 (MQ=255)
                                                             cTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGat                                       <  1:1148075/100‑1 (MQ=255)
                                                             cTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGat                                       <  1:960583/100‑1 (MQ=255)
                                                             cTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGat                                       <  1:1263629/100‑1 (MQ=255)
                                                               ccTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGatat                                     >  2:1373403/1‑100 (MQ=255)
                                                                cTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATc                                    >  2:801261/1‑100 (MQ=255)
                                                                  tAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCtt                                  <  1:2329941/100‑1 (MQ=255)
                                                                  tAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCtt                                  >  1:221288/1‑100 (MQ=255)
                                                                     tGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCAt                               >  1:322507/1‑100 (MQ=255)
                                                                         cGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCtt                           >  1:1112494/1‑100 (MQ=255)
                                                                          gTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTg                          <  1:1777094/100‑1 (MQ=255)
                                                                          gTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTg                          <  2:2050897/100‑1 (MQ=255)
                                                                             gtttATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTTCTCGATATCTTCATTCTTGCgg                       <  2:1381592/98‑1 (MQ=255)
                                                                              gTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCg                        <  2:2228501/98‑1 (MQ=255)
                                                                               ttATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCt                     <  1:1497415/100‑1 (MQ=255)
                                                                               ttATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCAACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCt                     <  2:702181/100‑1 (MQ=255)
                                                                                  tGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTaaa                  >  1:1687302/1‑100 (MQ=255)
                                                                                    cATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgc                <  1:1389945/100‑1 (MQ=255)
                                                                                     atcactaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcg               >  1:1524944/7‑100 (MQ=39)
                                                                                     aTAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcg               >  2:1363132/1‑100 (MQ=255)
                                                                                     aTAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcg               >  2:1735778/1‑100 (MQ=255)
                                                                                      tcactaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcgg              >  2:1073446/6‑100 (MQ=255)
                                                                                       cattaaGGCCGCGTAAGCGGGCTTAGTGATTACACGTTGTATCAATGATGTTCGATAGTATGGGTGTGCTCGATATATTCAATCTCGAGACTAAAgcag              >  2:1267614/5‑97 (MQ=255)
                                                                                       cactaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggc             >  2:1389958/5‑100 (MQ=255)
                                                                                       cactaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggc             >  1:2145132/5‑100 (MQ=255)
                                                                                       cactaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggc             >  1:2341024/5‑100 (MQ=255)
                                                                                       cactaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggc             >  1:694189/5‑100 (MQ=255)
                                                                                        actaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATCTCTTCATTCTTGCGGCTAAAgcg               >  2:1086920/4‑97 (MQ=255)
                                                                                        actaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcg            >  1:807495/4‑100 (MQ=255)
                                                                                        actaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggc             >  2:278507/4‑99 (MQ=255)
                                                                                        actaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATAGCTTCATTCTTGCGGCTAAAgcggcg            >  1:596964/4‑100 (MQ=255)
                                                                                         ctaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcgg           >  1:1657031/3‑100 (MQ=255)
                                                                                         ctaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggc             >  2:1329373/3‑98 (MQ=255)
                                                                                          aaaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcggc          <  1:1527129/100‑1 (MQ=255)
                                                                                           aaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcggcg         >  2:173593/1‑100 (MQ=255)
                                                                                           aaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcggc          >  2:2101492/1‑99 (MQ=255)
                                                                                            aGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGa        <  1:843294/100‑1 (MQ=255)
                                                                                             ggCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGaa       >  1:2227462/1‑100 (MQ=255)
                                                                                             ggCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGaa       >  1:148404/1‑100 (MQ=255)
                                                                                               ccGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAacc     <  1:310757/100‑1 (MQ=255)
                                                                                               ccGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAacc     <  1:2071273/100‑1 (MQ=255)
                                                                                               ccGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAacc     >  1:2200997/1‑100 (MQ=255)
                                                                                               ccGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAacc     >  2:797394/1‑100 (MQ=255)
                                                                                                cGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAacca    >  2:619512/1‑100 (MQ=255)
                                                                                                  cTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGCCAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAaccacc  >  2:1362172/1‑100 (MQ=255)
                                                                                                  cTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAaccacc  >  2:620102/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
TGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACC  >  NC_000913/481126‑481323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: