Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,077,116	T→C	100%	E589E (GAA→GAG)	putA ←	fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,077,116	0	T	C	100.0%	229.0 / NA	66	E589E (GAA→GAG)	putA	fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/37);  total (29/37)

CCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCC  >  NC_000913/1077020‑1077209                                                                                                 |                                                                                              cccTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTACGCCAGTTTctct                                                                                            <  1:1619099/100‑1 (MQ=255)     gCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTAcag                                                                                        >  2:721910/1‑100 (MQ=255)     gCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTAcag                                                                                        <  1:526560/100‑1 (MQ=255)     gCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTAcag                                                                                        >  1:2229822/1‑100 (MQ=255)      ccGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGTAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTAcagc                                                                                       <  2:1676317/100‑1 (MQ=255)        gAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTAcagcag                                                                                     <  1:108416/100‑1 (MQ=255)          gTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTg                                                                                   <  1:1900140/100‑1 (MQ=255)           ttGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGa                                                                                  <  2:1057809/100‑1 (MQ=255)           ttGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGa                                                                                  <  1:1777728/100‑1 (MQ=255)            tGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGaa                                                                                 >  2:1777714/1‑99 (MQ=255)             gTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTTCGCCAGTTTCTCTACAGCAGTGAcc                                                                                >  2:2194016/1‑100 (MQ=255)                    ccGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCgg                                                                         <  1:1940211/100‑1 (MQ=255)                    ccGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCgg                                                                         <  1:1980987/100‑1 (MQ=255)                     cGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGc                                                                        <  1:1350863/100‑1 (MQ=255)                      gTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCg                                                                       <  2:1145114/100‑1 (MQ=255)                        gACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGAc                                                                     <  1:2129682/100‑1 (MQ=255)                         aCCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGAcc                                                                    <  1:869434/100‑1 (MQ=255)                          ccGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCa                                                                   >  2:1399140/1‑100 (MQ=255)                           cGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGAcc                                                                    <  2:1403151/98‑1 (MQ=255)                             tAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGtt                                                                <  1:498669/100‑1 (MQ=255)                              aaaGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTc                                                               <  2:397600/100‑1 (MQ=255)                                aGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCAt                                                             <  1:811160/100‑1 (MQ=255)                                aGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCAt                                                             <  1:132395/100‑1 (MQ=255)                                     gcgcgGCAGGGGAAGTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATcc                                                           <  2:824790/97‑1 (MQ=255)                                      cgcgGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTg                                                       <  1:1716166/100‑1 (MQ=255)                                      cgcgGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTg                                                       <  2:1585467/100‑1 (MQ=255)                                       gcgGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTgg                                                      <  2:1705730/100‑1 (MQ=255)                                        cgGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGc                                                     >  1:2062964/1‑100 (MQ=255)                                          gCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCaa                                                   <  1:509576/100‑1 (MQ=255)                                            aGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAag                                                 <  1:1150747/100‑1 (MQ=255)                                            aGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTGCATGTTGCGCCAGGTGCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCaaa                                                  >  2:1746496/1‑99 (MQ=255)                                             ggggAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAaga                                                <  1:285476/100‑1 (MQ=255)                                             ggggAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAaga                                                >  1:1642441/1‑100 (MQ=255)                                               ggAATTTTCGGATGCGGTAATCTAGTTTGCCGTTCCTCTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAgg                                              <  2:1608637/100‑1 (MQ=255)                                               ggAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAgg                                              <  1:810536/100‑1 (MQ=255)                                               ggAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAgg                                              <  2:1077141/100‑1 (MQ=255)                                               ggAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAgg                                              <  2:1380731/100‑1 (MQ=255)                                               ggAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAgg                                              >  2:586149/1‑100 (MQ=255)                                                 aaTTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGgtg                                            <  1:60021/100‑1 (MQ=255)                                                  aTTTTCGGATGCGGTAATCGAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGgtgt                                           >  2:388841/1‑100 (MQ=255)                                                     ttCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCCGTGGCAAAGAGGTGTCgg                                        >  2:1983109/1‑100 (MQ=255)                                                     ttCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCgg                                        <  2:2042598/100‑1 (MQ=255)                                                      tCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGc                                       <  1:849139/100‑1 (MQ=255)                                                      tCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGc                                       <  1:488358/100‑1 (MQ=255)                                                        ggATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTAAAGCAGTGACCGGCTCGGCGCCCAGTTCGTATAGTGGCAAAGAGGTGTAGGCa                                      >  2:107469/1‑99 (MQ=255)                                                           tGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATAc                                  >  1:535706/1‑100 (MQ=255)                                                             cGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCACACAGGTGTCGGCAATAc                                  >  2:694959/1‑98 (MQ=255)                                                                tAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTa                             <  2:957461/100‑1 (MQ=255)                                                                 aaTCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTaa                            >  2:2054849/1‑100 (MQ=255)                                                                   tCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACa                          >  1:1398248/1‑100 (MQ=255)                                                                    ccAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACaa                         >  2:936056/1‑100 (MQ=255)                                                                    ccAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACaa                         >  2:255317/1‑100 (MQ=255)                                                                       gTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACg                      >  1:1830001/1‑100 (MQ=255)                                                                            cccTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGgtg                 >  1:1164429/1‑100 (MQ=255)                                                                              cTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGtt               >  1:411063/1‑100 (MQ=255)                                                                              cTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGtt               >  2:631488/1‑100 (MQ=255)                                                                               ttCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTa              >  2:566357/1‑100 (MQ=255)                                                                                  cTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCa           >  1:749987/1‑100 (MQ=255)                                                                                   tGTTGCGGCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCAc          <  2:2133831/100‑1 (MQ=255)                                                                                     ttGAGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCg        <  2:308005/100‑1 (MQ=255)                                                                                      tGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGt       <  1:311126/100‑1 (MQ=255)                                                                                       gcgcCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGtt      >  1:1417033/1‑100 (MQ=255)                                                                                       gcgcCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGtt      >  2:1125582/1‑100 (MQ=255)                                                                                          ncAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTc   >  2:1588666/2‑100 (MQ=255)                                                                                          ccAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTc   >  2:1433129/1‑100 (MQ=255)                                                                                           cAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTcc  >  2:2176447/1‑100 (MQ=255)                                                                                           cAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTcc  <  2:1317278/100‑1 (MQ=255)                                                                                                 |                                                                                              CCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCC  >  NC_000913/1077020‑1077209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑