Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,146,997	G→A	100%	T68T (ACG→ACA)	yceD →	DUF177 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,146,997	0	G	A	100.0%	318.3 / ‑6.7	94	T68T (ACG→ACA)	yceD	DUF177 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (45/48);  total (45/49)

GTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGC  >  NC_000913/1146908‑1147095                                                                                          |                                                                                                   tccgatctgGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCg                                                                                          <  2:312539/93‑1 (MQ=255) gTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCg                                                                                          >  2:916557/1‑100 (MQ=255) gTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCg                                                                                          >  2:2136785/1‑100 (MQ=255)   cAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAg                                                                                        <  1:1640736/100‑1 (MQ=255)   cAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAg                                                                                        <  1:1148839/100‑1 (MQ=255)   cAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTAGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAg                                                                                        <  1:1673426/100‑1 (MQ=255)    aGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGTGAAGGTGACAGTAACGCTCGAgt                                                                                       >  1:978468/1‑100 (MQ=255)     gtgtGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAgtg                                                                                      <  2:730692/100‑1 (MQ=255)        tgGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCa                                                                                   >  1:583625/1‑100 (MQ=255)        tgGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCa                                                                                   <  2:442262/100‑1 (MQ=255)         ggACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAgatcgga                                                                                  >  1:312541/1‑94 (MQ=255)         ggACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAg                                                                                  >  2:169056/1‑100 (MQ=255)         ggACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAg                                                                                  >  2:875689/1‑100 (MQ=255)          gACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGc                                                                                 <  1:65158/100‑1 (MQ=255)              gTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTg                                                                             <  1:1932622/100‑1 (MQ=255)               tGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGc                                                                            <  2:116565/100‑1 (MQ=255)               tGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGc                                                                            <  1:2149974/100‑1 (MQ=255)                 aTGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCgg                                                                          <  1:660037/100‑1 (MQ=255)                 aTGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCgg                                                                          <  1:395708/100‑1 (MQ=255)                  tgtgGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCggg                                                                         <  2:1239528/100‑1 (MQ=255)                  tgtgGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCggg                                                                         <  2:591128/100‑1 (MQ=255)                  tgtgGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCggg                                                                         >  2:1796032/1‑100 (MQ=255)                  tgtgGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCgg                                                                          <  2:2114963/99‑1 (MQ=255)                   gtgGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCggg                                                                         >  2:2125126/1‑99 (MQ=255)                   gtgGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGa                                                                        >  1:719603/1‑100 (MQ=255)                     ggAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAg                                                                      <  2:65119/100‑1 (MQ=255)                     ggAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAg                                                                      >  1:1262500/1‑100 (MQ=255)                         tGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGt                                                                  >  1:88322/1‑100 (MQ=255)                         tGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGt                                                                  >  1:1418793/1‑100 (MQ=255)                          gCTCCATGTCGTTCGCTATCTATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGtt                                                                 <  2:421894/100‑1 (MQ=255)                          gCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGtt                                                                 >  1:1943528/1‑100 (MQ=255)                          gCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGtt                                                                 <  1:1154635/100‑1 (MQ=255)                           cTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGttt                                                                >  2:101245/1‑100 (MQ=255)                           cTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGttt                                                                >  2:1907883/1‑100 (MQ=255)                            tCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTa                                                               >  2:797402/1‑100 (MQ=255)                              cATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACt                                                             >  2:1681576/1‑100 (MQ=255)                              cATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACt                                                             >  2:1681586/1‑100 (MQ=255)                                tGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACtca                                                           <  2:1551682/100‑1 (MQ=255)                                tGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACtca                                                           <  2:841806/100‑1 (MQ=255)                                agTCGTTCGCTATCGGTAACGAACGTCTCGCAGTGTTAAACGGCGCTGCGAAGGTGACAGTAACGATCGAGTGTCAGCGTTGAGGGAAGGAGTTTa                                                               >  2:411605/2‑96 (MQ=255)                                 gTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACtcat                                                          >  2:1968422/1‑100 (MQ=255)                                   cGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACtcatca                                                        <  2:222095/100‑1 (MQ=255)                                    gTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAg                                                       <  1:147878/100‑1 (MQ=255)                                    gTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAg                                                       >  1:2360997/1‑100 (MQ=255)                                        gCTATCGATAAGCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCt                                                   <  2:189952/100‑1 (MQ=255)                                        gCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCt                                                   >  1:1200307/1‑100 (MQ=255)                                        gCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCt                                                   <  1:2350280/100‑1 (MQ=255)                                           aTCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTaca                                                <  1:1926131/100‑1 (MQ=255)                                           aTCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTaca                                                <  1:1346576/100‑1 (MQ=255)                                            tCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTacac                                               >  1:1843995/1‑100 (MQ=255)                                                tAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGTCGTTTATTCATCAGGTCTACACAACg                                           <  2:1563259/100‑1 (MQ=255)                                                 aaCCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGt                                          <  1:2257708/100‑1 (MQ=255)                                                  aCCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAAAGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAAAGt                                          >  2:2265079/1‑99 (MQ=255)                                                    cAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTAtt                                       <  1:797404/100‑1 (MQ=255)                                                     aaCGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTg                                      >  1:837668/1‑100 (MQ=255)                                                      aCGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGt                                     >  2:562356/1‑100 (MQ=255)                                                       cGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGtt                                    <  2:837666/100‑1 (MQ=255)                                                        gTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGttt                                   <  2:864632/100‑1 (MQ=255)                                                        gTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGttt                                   <  1:285677/100‑1 (MQ=255)                                                        gTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGttt                                   <  1:2194141/100‑1 (MQ=255)                                                         tctcGCAGTGTTAAACGTCGATGCGAAGGTGACATTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGtttt                                  <  2:1013004/100‑1 (MQ=255)                                                         tctcGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAATCCGTTTACTCATCAGGTCTACACAACGTATTGtttt                                  <  1:1681599/100‑1 (MQ=255)                                                         tctcGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGtttt                                  <  1:706777/100‑1 (MQ=255)                                                         tctcGCAGTGTTAAACGGCGATGCGAAGGTGACAGCAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGtttt                                  <  1:1681589/100‑1 (MQ=255)                                                          ctcGCAGTGTTAAGCGGTGATGCGAAGGGGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGttt                                   <  2:1556942/98‑1 (MQ=255)                                                          ctcGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTa                                 >  1:1445044/1‑100 (MQ=255)                                                          ctcGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTa                                 <  1:35483/100‑1 (MQ=255)                                                            cGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGt                               >  1:1066367/1‑100 (MQ=255)                                                            cGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGt                               >  1:1143970/1‑100 (MQ=255)                                                             gCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTc                              <  2:1841302/100‑1 (MQ=255)                                                              cAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTcc                             <  2:1584097/100‑1 (MQ=255)                                                              cAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTcc                             >  1:2195417/1‑100 (MQ=255)                                                                gtgtTAAACGGCGATGCGAAGGTGACAGTCACGCTCGAGCGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTTTACACAACGTATTGTTTTAGTCCtg                           >  1:225743/1‑100 (MQ=255)                                                                  gtTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCtgtg                         >  1:1990635/1‑100 (MQ=255)                                                                   ttAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGc                        >  1:2068670/1‑100 (MQ=255)                                                                    tAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGGGGGAAGCCGTTTACTCATCAGGTCTACAAAACGTATTGTTTTAGTCCTGTGCg                       >  2:152669/1‑100 (MQ=255)                                                                    tAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCg                       >  2:514036/1‑100 (MQ=255)                                                                     aaaCGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAgatcggaagag                      >  1:266925/1‑90 (MQ=255)                                                                     aaaCGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGt                      >  1:1576443/1‑100 (MQ=255)                                                                           cGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGAc                <  2:924592/100‑1 (MQ=255)                                                                           cGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGAc                <  1:2300884/100‑1 (MQ=255)                                                                            gATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACg               <  1:1489084/100‑1 (MQ=255)                                                                              tGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGCAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTTCGTTCAGACGaa             >  2:1860405/1‑100 (MQ=255)                                                                               gCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAAc            >  2:2339694/1‑100 (MQ=255)                                                                               gCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAAc            >  2:1028276/1‑100 (MQ=255)                                                                               gCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAAc            <  1:1340125/100‑1 (MQ=255)                                                                               gCGAAGGTGACAGTAACGCTCGAGTGCCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAAc            >  2:2372821/1‑100 (MQ=255)                                                                                cGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACa           >  1:707431/1‑100 (MQ=255)                                                                                 gAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAg          >  1:110972/1‑100 (MQ=255)                                                                                   aGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGc        <  2:1418785/100‑1 (MQ=255)                                                                                   aGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGc        <  1:1565283/100‑1 (MQ=255)                                                                                   aGGGGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCGACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGc        <  2:833420/100‑1 (MQ=255)                                                                                      tGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGa     >  1:175152/1‑100 (MQ=255)                                                                                         cGGTAACGCTCGAGTGTCAGCGTTGCTGGAAGCAGTTTCCTCATCAGTTCTACAAAACGTATTGTTTTAGTCCTGGGCATTCAGACGCACAGGCTGAAgt  <  2:1098551/100‑2 (MQ=255)                                                                                          |                                                                                                   GTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGC  >  NC_000913/1146908‑1147095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑