Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,174,080 T→C 16.3% intergenic (‑116/+12) mfd ← / ← ycfT transcription‑repair coupling factor/inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,174,0800TC16.3% 279.0 / 43.1 122intergenic (‑116/+12)mfd/ycfTtranscription‑repair coupling factor/inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (51/51);  new base C (8/12);  total (59/63)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 122 total aligned reads displayed.

GTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAG  >  NC_000913/1173983‑1174178
                                                                                                 |                                                                                                  
gTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTgg                                                                                                  <  2:2284075/100‑1 (MQ=255)
   aGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGc                                                                                               <  1:1893649/100‑1 (MQ=255)
   aGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGc                                                                                               <  2:1843094/100‑1 (MQ=255)
    ggATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCg                                                                                              >  1:2368929/1‑100 (MQ=255)
      atatGCCTCACCATATGGGGGCATATCTCTGATAGTCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCAGCCGGGGCGAt                                                                                            <  2:2130480/100‑1 (MQ=255)
      atatGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGAt                                                                                            <  2:882708/100‑1 (MQ=255)
       tatGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATg                                                                                           <  2:1743261/100‑1 (MQ=255)
       tatGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATg                                                                                           <  1:2062690/100‑1 (MQ=255)
       tatGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATg                                                                                           <  2:193620/100‑1 (MQ=255)
        atGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCAGCCGGGGCGAttc                                                                                          <  1:335055/100‑3 (MQ=255)
        atGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCAGCCGGGGCGAttc                                                                                          <  1:1271268/100‑3 (MQ=255)
        atGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCAGCCGGGGCGAttc                                                                                          <  1:1270985/100‑3 (MQ=255)
         tGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGc                                                                                         >  2:591694/1‑100 (MQ=255)
         tGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCAGCCGGGGCGAttca                                                                                         <  2:1817135/100‑4 (MQ=255)
          gCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCt                                                                                        >  1:467820/1‑100 (MQ=255)
          gCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCt                                                                                        >  1:2132157/1‑100 (MQ=255)
          gCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCt                                                                                        >  1:1939295/1‑100 (MQ=255)
          gCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCAGCCGGGGCGAttcaa                                                                                        <  1:1581784/100‑5 (MQ=255)
          gCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCAGCCGGGGCGAttcaa                                                                                        <  1:431445/100‑5 (MQ=255)
          gCCTCAACATATGGGAGCATATGTCAGATAAGCAATGTTGTTCATAATCCGGAATCGACTTGGTTTAGCAAATTGAATCGCCCCGGCTGGGGTGCTgg                                                                                          <  2:1591835/98‑1 (MQ=255)
           ccTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCtt                                                                                       >  2:782498/1‑100 (MQ=255)
           ccTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCtt                                                                                       >  1:1659911/1‑100 (MQ=255)
           ccTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGGTTAGCCAATTGAATGGCCCCGGCTGGGGCGATGGCtt                                                                                       >  2:1607674/1‑100 (MQ=255)
           ccTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCACTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCAGCCGGGGCGAttcaat                                                                                       <  1:339543/100‑6 (MQ=255)
             tCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCAGCCGGGGCGAttcaattt                                                                                     <  1:472201/100‑8 (MQ=38)
              cAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTTATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCAGCCGGGGCGAttcaatttg                                                                                    <  1:1012656/100‑9 (MQ=38)
              cAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGc                                                                                    >  1:1111664/1‑100 (MQ=255)
              cAACATAAGAGGGCAGTTCTCGGATAAGCAATGCTGTTCATTATCCGGAAACGACGTAGTTTAGCAAATTGAAGCGCCCCAGCCGCGGCGAt                                                                                            <  2:2076220/92‑1 (MQ=255)
                aCATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCgg                                                                                  <  2:1646913/100‑1 (MQ=255)
                aCATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCgg                                                                                  <  1:1698269/100‑1 (MQ=255)
                 cATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCggg                                                                                 <  1:180836/100‑1 (MQ=255)
                     tGGGGGCATATCTCTGATAAGCAATGCTGTTCCTTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTa                                                                             >  1:2163530/1‑100 (MQ=255)
                      gggggCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAGTCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTaa                                                                            >  2:1077520/1‑100 (MQ=255)
                        gggCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACt                                                                          >  2:1256753/1‑100 (MQ=255)
                         ggCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTg                                                                         >  2:1049287/1‑100 (MQ=255)
                         ggCATATCACTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTAAGGCGATGGCTTAGCGGGAGTAAc                                                                           >  2:333460/1‑98 (MQ=255)
                          gCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGa                                                                        <  2:841039/100‑1 (MQ=255)
                           cATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGAc                                                                       <  2:697231/100‑1 (MQ=255)
                              atCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGc                                                                    >  1:2295400/1‑100 (MQ=255)
                               tctcTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCa                                                                   >  1:674404/1‑100 (MQ=255)
                                ctctGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAg                                                                  >  1:246228/1‑100 (MQ=255)
                                 tctGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGc                                                                 <  1:1029936/100‑1 (MQ=255)
                                 tctGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGc                                                                 <  1:1697149/100‑1 (MQ=255)
                                   tGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCgg                                                               >  1:2124360/1‑100 (MQ=255)
                                   tGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCgg                                                               >  2:1646611/1‑100 (MQ=255)
                                   tGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCgg                                                               <  2:2377308/100‑1 (MQ=255)
                                     aTAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGc                                                             <  1:2260203/100‑1 (MQ=255)
                                      tAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCa                                                            <  2:2008545/100‑1 (MQ=255)
                                       aaGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAg                                                           >  1:2189166/1‑100 (MQ=255)
                                          cAATGCTGTTCATTATCCGTAATCGACATGGTTTACCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGa                                                        <  2:1166621/100‑1 (MQ=255)
                                            aTGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGTCAGCGGGCAGTGAAg                                                      >  1:2348862/1‑100 (MQ=255)
                                              gCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGc                                                    <  1:138433/100‑1 (MQ=255)
                                               cTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCg                                                   <  2:1231531/100‑1 (MQ=255)
                                                tGTTCATTATCCGTAATCGACATGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCggg                                                 >  2:2275938/1‑100 (MQ=255)
                                                 gTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCggg                                                 >  1:641891/1‑100 (MQ=255)
                                                  ttCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCgggg                                                <  1:1150560/100‑1 (MQ=255)
                                                     aTTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGgaga                                             >  1:963983/1‑100 (MQ=255)
                                                       tATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGaac                                           <  2:564974/100‑1 (MQ=255)
                                                        ntCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGaaca                                          >  1:684611/2‑100 (MQ=255)
                                                           cGTAATCGACATGGTTTAGCAAATTGAATCACCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAacaaca                                       <  1:1891746/100‑1 (MQ=255)
                                                              aaTCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGCGaacaac                                        >  2:1906753/1‑96 (MQ=255)
                                                                 cGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGc                                 <  1:2253210/100‑1 (MQ=255)
                                                                  gACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCt                                <  2:246226/100‑1 (MQ=255)
                                                                  gACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCt                                >  1:1886393/1‑100 (MQ=255)
                                                                   aCATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTg                               <  1:249134/100‑1 (MQ=255)
                                                                   aCATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAAAAACAGATCGCTg                               >  2:192934/1‑100 (MQ=255)
                                                                    cATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGa                              >  2:475046/1‑100 (MQ=255)
                                                                     aTGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGaa                             <  1:309014/100‑1 (MQ=255)
                                                                      tGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAAt                            <  1:703195/100‑1 (MQ=255)
                                                                       ggTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATg                           <  1:656470/100‑1 (MQ=255)
                                                                       ggTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATg                           <  2:2171482/100‑1 (MQ=255)
                                                                        gTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGc                          >  1:2094496/1‑100 (MQ=255)
                                                                         tttAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCa                         >  1:67734/1‑100 (MQ=255)
                                                                         tttAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCa                         >  2:2150562/1‑100 (MQ=255)
                                                                          ttAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCAc                        >  2:1042839/1‑100 (MQ=255)
                                                                             gCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCt                     >  2:2262249/1‑100 (MQ=255)
                                                                              cAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCtg                    >  2:355251/1‑100 (MQ=255)
                                                                               ctaagccaTCGCCCCAGCCGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCtg                    >  1:2135283/8‑99 (MQ=38)
                                                                               aaaTTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCtgt                   <  1:1861223/100‑1 (MQ=255)
                                                                                aaTTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCtgtg                  <  2:2356954/100‑1 (MQ=255)
                                                                                  ttGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGTGGGGAGAACAACAGATCGCTGAATGCACGCTGTGaa                <  1:1683676/100‑1 (MQ=255)
                                                                                  ttGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGaa                >  1:1779946/1‑100 (MQ=255)
                                                                                  agccaTCGCCCCAGCCGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGAGCGCTGAATGCACGCTGGGa                 >  2:831885/5‑99 (MQ=255)
                                                                                   gccaTCGCCCCAGCCGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGaaa               >  1:1785723/4‑100 (MQ=255)
                                                                                   gccaTCGCCCCAGCCGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGaaa               >  1:888002/4‑100 (MQ=255)
                                                                                    gAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAg              <  1:1079627/100‑1 (MQ=255)
                                                                                    ccaTCGCCCCAGCCGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAg              >  1:241236/3‑100 (MQ=255)
                                                                                     caTCGCCCCAGCCGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGt             >  2:1489352/2‑100 (MQ=255)
                                                                                     aaTCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGt             <  1:1234332/100‑1 (MQ=255)
                                                                                      aTCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGtt            >  1:544473/1‑100 (MQ=255)
                                                                                      aTCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGtt            >  2:221736/1‑100 (MQ=255)
                                                                                         gCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGCATGCACGCTGTGAAAGTTTTc         >  2:1234730/1‑100 (MQ=255)
                                                                                         gCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAGAGTTTTc         <  1:1254168/100‑1 (MQ=255)
                                                                                         gCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTc         <  1:612380/100‑1 (MQ=255)
                                                                                         gCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTc         <  2:2306790/100‑1 (MQ=255)
                                                                                         gCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTc         >  1:1611062/1‑100 (MQ=255)
                                                                                         gCCCCAGCCGGGGCGATCGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGCGCAGGCGGGGAGAACAACAGATCGCTGAATGCACGCCGTGAAAGtttt          >  2:11093/1‑99 (MQ=255)
                                                                                          ccccGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCt        <  2:466351/100‑1 (MQ=255)
                                                                                          ccccGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCt        <  2:169890/100‑1 (MQ=255)
                                                                                               gCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCa   >  1:2016507/1‑100 (MQ=255)
                                                                                                 |                                                                                                  
GTTAGGATATGCCTCAACATATGGGGGCATATCTCTGATAAGCAATGCTGTTCATTATCCGTAATCGACATGGTTTAGCAAATTGAATCGCCCCGGCTGGGGCGATGGCTTAGCGGGAGTAACTGACGGCAGCGGGCAGTGAAGGCGGGGAGAACAACAGATCGCTGAATGCACGCTGTGAAAGTTTTCTTACCAG  >  NC_000913/1173983‑1174178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: