Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,312,192	T→C	100%	M19V (ATG→GTG)	yciA ←	acyl‑CoA esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,312,192	0	T	C	100.0%	232.6 / ‑6.7	70	M19V (ATG→GTG)	yciA	acyl‑CoA esterase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (32/35);  minor base G (1/1);  total (34/36)

GGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTAC  >  NC_000913/1312096‑1312288                                                                                                 |                                                                                                 gggCAATTTCTTTTGCCAGAATAGCGCCGCCACTATCCATGTGTGACATTAACCAGCCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGc                                                                                               <  2:2275370/100‑1 (MQ=255) gggCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGc                                                                                               <  1:1172484/100‑1 (MQ=255)  gtcAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGc                                                                                               <  2:188769/97‑1 (MQ=255)  ggCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCt                                                                                              >  1:851675/1‑100 (MQ=255)   gCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTa                                                                                             >  1:103013/1‑100 (MQ=255)   gCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTa                                                                                             >  1:718444/1‑100 (MQ=255)   gCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTa                                                                                             >  1:1768885/1‑100 (MQ=255)      aTTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAg                                                                                          <  1:598295/100‑1 (MQ=255)        ttCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTa                                                                                        <  1:223154/100‑1 (MQ=255)           ttttGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGt                                                                                     <  1:1653132/100‑1 (MQ=255)           ttttGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGt                                                                                     <  2:1418549/100‑1 (MQ=255)           ttttGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTAACGGCGGGCAAGGCTAAAGTaa                                                                                       >  2:2315944/1‑97 (MQ=255)              tGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTaaa                                                                                  >  2:228802/1‑100 (MQ=255)              tGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTaaa                                                                                  >  1:308670/1‑100 (MQ=255)               gCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTaaaa                                                                                 >  1:2108663/1‑100 (MQ=255)               atctgAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTaaaa                                                                                 <  1:2345615/96‑1 (MQ=255)                 tccgatctcgccgcCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACa                                                                               <  2:335136/92‑1 (MQ=255)                   gAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTaaaaagat                                                                             >  2:2345594/1‑96 (MQ=255)                   gAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAg                                                                             >  1:15591/1‑100 (MQ=255)                     aTAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGAt                                                                           <  2:1705036/100‑1 (MQ=255)                       aGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCg                                                                         >  1:338238/1‑100 (MQ=255)                       aGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCg                                                                         >  1:549623/1‑100 (MQ=255)                         cgccgcCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCgga                                                                       >  1:335138/1‑98 (MQ=255)                         cgccgcCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACAGCTAAAGTACGTAAAACAAGATCGcc                                                                       >  2:821551/1‑100 (MQ=255)                          gccgccAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGccc                                                                      <  1:2207411/100‑1 (MQ=255)                          gccgccAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGccc                                                                      <  1:1484691/100‑1 (MQ=255)                           ccgccAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCt                                                                     <  1:2321027/100‑1 (MQ=255)                             gccAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGa                                                                   <  1:2300352/100‑1 (MQ=255)                              ccAATATCCATTTGTGACATTAACCAACCACCAGAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGTTCGCCCTGAg                                                                  >  2:1561721/1‑100 (MQ=255)                                   atCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATAGCCCTGAGGGAc                                                              >  2:1985562/1‑99 (MQ=255)                                     ccATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGtt                                                           >  1:2331933/1‑100 (MQ=255)                                     ccATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGtt                                                           >  1:809817/1‑100 (MQ=255)                                        tttGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTAtg                                                        <  1:839056/100‑1 (MQ=255)                                          tgtgACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTAtgtg                                                      <  1:1454525/100‑1 (MQ=255)                                           gtgACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATgtgt                                                     >  1:471174/1‑100 (MQ=255)                                           gtgACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATgtgt                                                     >  1:26315/1‑100 (MQ=255)                                           gtgACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATgtgt                                                     >  2:1595254/1‑100 (MQ=255)                                           gtgACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATgtgt                                                     <  2:1711656/100‑1 (MQ=255)                                            tgACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGtgtt                                                    >  2:1133942/1‑100 (MQ=255)                                              aCATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGtgttgt                                                  <  2:549621/100‑1 (MQ=255)                                              aCATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGtgttgt                                                  <  2:338236/100‑1 (MQ=255)                                                aTTAACCAACCCCCAAAGATGTCACCATTGGCATTGGTATCAGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAg                                                <  2:1759508/100‑1 (MQ=255)                                                     ccaaccaCCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACa                                             >  2:2171345/1‑98 (MQ=255)                                                      caaccaCCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATgg                                          >  1:616397/1‑100 (MQ=255)                                                        accaccAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTTTGTGTTGTAGACATGGTa                                        <  2:2253188/100‑1 (MQ=255)                                                        accaccAAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTa                                        <  2:1478480/100‑1 (MQ=255)                                                           acccAAGATGTTACTATTGGCATTGGTATCGACTGGCAGGGATAACGCACGCAAAACAAGATCGCCCTGCTGGCCGTTATGTGTTGTAGACATGGTaa                                       >  2:888210/1‑98 (MQ=255)                                                           accaAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTaaaa                                     >  2:1106503/1‑100 (MQ=255)                                                            ccaAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGACCTGAGGGACGTTATGTGTTGTAGAAATGGTa                                        >  1:196654/1‑96 (MQ=255)                                                             caAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAAcc                                   >  2:641994/1‑100 (MQ=255)                                                             caAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAAcc                                   <  1:166392/100‑1 (MQ=255)                                                             caAAGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAAcc                                   <  1:423474/100‑1 (MQ=255)                                                              aaaGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCg                                  >  2:972977/1‑100 (MQ=255)                                                                aGATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGAc                                <  1:1915974/100‑1 (MQ=255)                                                                 gATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACt                               <  1:972980/100‑1 (MQ=255)                                                                 gATGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACt                               <  1:1893522/100‑1 (MQ=255)                                                                   tGTCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATAGCCCTGGGGGACGCTATGTGGTGTAGACATGGCCAAACCGACt                               >  2:647162/1‑98 (MQ=255)                                                                     ttaCCATTGGCATTGGTATCGGCGGGCAGGGCTAAAGTACGTAAATCAATATCGCCCTGAGTGACGTTATGTGTTGTAGACATGGTAAATCCGACGTa                             <  2:1668685/96‑1 (MQ=255)                                                                     tCACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTaaa                           <  2:69526/100‑1 (MQ=255)                                                                      cACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTaaaa                          <  2:103012/100‑1 (MQ=255)                                                                      cACCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACAAGGTAAAACCGACTTaaaa                          >  2:186858/1‑100 (MQ=255)                                                                       aCCATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAg                         <  1:1857480/100‑1 (MQ=255)                                                                        ccATTGGCATTGGTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAgg                        >  2:1342372/1‑100 (MQ=255)                                                                                 ttggTATCGGCGGGCACGGCTAAAGTAAGTAAAACAAGATCGCCCTGAGGGACGTCATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATcacc               <  2:792070/100‑2 (MQ=255)                                                                                   ggTATCGGCGGGCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAgg             >  2:973501/1‑100 (MQ=255)                                                                                    gTATCGGCCGGCACGGCTAAAGTGCGTAAAACAAAAGCGCCCTGAGAGACGTTATGTGTTGTAGACATGGTAAATCCGACTTAAAAGGAAAATca                 <  2:1554202/95‑1 (MQ=255)                                                                                            gggCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGct    <  1:2285436/100‑1 (MQ=255)                                                                                            gggCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGct    >  1:580190/1‑100 (MQ=255)                                                                                              gCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGctac  <  1:1002338/100‑1 (MQ=255)                                                                                              gCACGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTACGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGctac  <  1:1985579/100‑1 (MQ=255)                                                                                                 |                                                                                                 GGGCAATTTCTTTTGCCAGAATAGCGCCGCCAATATCCATTTGTGACATTAACCAACCACCAAAGATGTCACCATTGGCATTGGTATCGGCGGGCATGGCTAAAGTACGTAAAACAAGATCGCCCTGAGGGACGTTATGTGTTGTAGACATGGTAAAACCGACTTAAAAGGAAAATCACAGGCGCGATGCTAC  >  NC_000913/1312096‑1312288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑