| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,489,197 | C→T | 100% | D322D (GAC→GAT) | aldA → | aldehyde dehydrogenase A, NAD‑linked |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,489,197 | 0 | C | T | 98.3% | 194.5 / ‑4.4 | 62 | D322D (GAC→GAT) | aldA | aldehyde dehydrogenase A, NAD‑linked |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (32/28); total (33/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01 | |||||||||||
GTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCGG > NC_000913/1489102‑1489292 | gtTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTg > 2:1572623/1‑100 (MQ=255) tttATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGc < 1:1730699/100‑1 (MQ=255) ttATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGc < 2:1395587/99‑1 (MQ=255) ttATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCg > 1:1520275/1‑100 (MQ=255) tATGTAAAGAAAGGCATTTATGATAAGTTCGTCAATCGGCTGGGTGAATCGATGCAGGCGGTTAAAATCGGTAAGCCTGCTGAACTCAAGGAATTTGc > 2:532640/1‑98 (MQ=255) gTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATgg > 2:1536365/1‑100 (MQ=255) tACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATggg > 2:1886291/1‑100 (MQ=255) tACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATggg > 1:1434693/1‑100 (MQ=255) gCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGAt < 1:1536376/100‑1 (MQ=255) gCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGAt < 2:492248/100‑1 (MQ=255) cATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGAtt < 2:1803872/100‑1 (MQ=255) cATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGAtt < 1:2042488/100‑1 (MQ=255) aTTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTa > 1:892254/1‑100 (MQ=255) aTTTATGATCAGTTCGTCAATCGGCGGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTa < 2:1406552/100‑1 (MQ=255) tGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGCTTAAcgcc > 1:624388/1‑100 (MQ=255) gATCAGTTCGTCAATCGGCTGGGTGGAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAAcgccg < 2:2039574/100‑1 (MQ=255) aTCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAAcgccgc > 1:731207/1‑100 (MQ=255) aTCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAAcgccgc < 1:1888789/100‑1 (MQ=255) cAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCcgcg < 1:1568124/99‑1 (MQ=255) cGTCAATCGGCTGGGTGAAGCGATGAAGGCGGGACAATTAGGTAACCCCGATGAACGCAACGATATAGAGATGGGCCGGTAGCTTAACGCCGCGgcgc > 2:476159/1‑98 (MQ=255) gTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTgg > 2:415004/1‑100 (MQ=255) cAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGaa > 1:454871/1‑100 (MQ=255) aTCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAg > 1:2247233/1‑100 (MQ=255) cTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGATAGGGTCg < 1:1245015/100‑1 (MQ=255) cTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCg < 2:1520265/100‑1 (MQ=255) gTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCa > 2:480631/1‑100 (MQ=255) gTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCa > 1:1453145/1‑100 (MQ=255) tGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGGCGCGGCGCTGGAAGGGGTCGAGc > 1:995425/1‑98 (MQ=255) gAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCACTGGAAAGGGTCGAGCaaa > 1:2227234/1‑100 (MQ=255) gCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAg < 1:1207430/100‑1 (MQ=255) cGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGCTGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGt > 2:1018887/1‑100 (MQ=255) gATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAg > 2:1513887/1‑98 (MQ=255) gATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAg < 2:1777809/98‑1 (MQ=255) aTGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTgg < 1:135795/100‑1 (MQ=255) tGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGgc < 1:830177/100‑1 (MQ=255) tGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGgc > 1:1539530/1‑100 (MQ=255) gCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGgcg < 1:429178/100‑1 (MQ=255) cAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGgcgc > 2:1507826/1‑100 (MQ=255) cAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGgcgc > 1:209801/1‑100 (MQ=255) cAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGgcac < 1:1535102/100‑3 (MQ=255) cAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGC‑AAAAGTGgcgcg < 1:480633/100‑1 (MQ=255) cAGGAGGTTCAATTTGGTAACCCAGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTCGAAAAGGTCGAGCAAAAAGTGgcgc < 2:1992981/100‑1 (MQ=255) aGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGgcgcg < 1:397850/100‑1 (MQ=255) aGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGgcgcg < 2:710197/100‑1 (MQ=255) ttCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGt > 2:966755/1‑100 (MQ=255) ttCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGt > 1:2129259/1‑100 (MQ=255) aaTTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGAGTAGAGAAAAAAGTGGCGCGCGCAGTaga < 2:1751724/100‑1 (MQ=255) aTTTGGTAAAACCGCTGAACGCAACGATATTGCGATGGGGCAGTTGATTAACGCCGCGGCGCTGGAAAAAAAAAAGAAAAAAGTGGCGCGCGCAGTagaa < 2:939465/100‑1 (MQ=255) tttGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTagaag > 1:903900/1‑100 (MQ=255) aaCCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAgggg > 1:366662/1‑100 (MQ=255) aaCCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAgggg > 1:94657/1‑100 (MQ=255) aaCCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAgggg < 2:427439/100‑1 (MQ=255) ccccGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCg > 1:698256/1‑100 (MQ=255) ccccGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGAAGAAGAAGGGGCg > 1:1384869/1‑100 (MQ=255) gCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCg < 2:1483595/96‑1 (MQ=255) gAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGAGCTGGCAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTgg > 2:1712831/1‑100 (MQ=255) aCGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCg < 2:366660/100‑1 (MQ=255) aCGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCg < 2:94656/100‑1 (MQ=255) cAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGAGGCGTTc > 2:1830752/1‑100 (MQ=255) aCGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCgg > 2:2027053/1‑100 (MQ=255) aCGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCgg < 1:2338314/100‑1 (MQ=255) aCGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCgg > 1:1410242/1‑100 (MQ=255) | GTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCGG > NC_000913/1489102‑1489292 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |