Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,499,361 A→G 16.2% N142D (AAC→GAC)  rimL → ribosomal‑protein‑L7/L12‑serine acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,499,3610AG16.2% 168.0 / 25.7 74N142D (AAC→GAC) rimLribosomal‑protein‑L7/L12‑serine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (23/39);  new base G (7/5);  total (30/44)
Fisher's exact test for biased strand distribution p-value = 2.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01

ATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCG  >  NC_000913/1499262‑1499458
                                                                                                   |                                                                                                 
aTCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCa                                                                                                   <  1:366363/100‑1 (MQ=255)
   aTTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAAcc                                                                                                <  2:1198208/100‑1 (MQ=255)
   aTTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAAcc                                                                                                >  1:166688/1‑100 (MQ=255)
    tttCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCa                                                                                               >  2:2173319/1‑100 (MQ=255)
           ggCGCTGCAGGCATTGATTCATCATTACGCCCAATCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGc                                                                                         >  2:1575394/1‑99 (MQ=255)
                tGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcg                                                                                   <  1:288479/100‑1 (MQ=255)
                tGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTgcg                                                                                   <  1:2209353/100‑1 (MQ=255)
                tGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACGTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTgcg                                                                                   <  1:925016/100‑1 (MQ=255)
                 gCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcgg                                                                                  <  2:982150/100‑2 (MQ=39)
                 gCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcgg                                                                                  <  1:810937/100‑2 (MQ=39)
                    ggCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAAt                                                                               >  1:2120608/1‑100 (MQ=255)
                     gCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcggattg                                                                              <  1:2058197/100‑6 (MQ=38)
                      cATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATgg                                                                             <  1:711914/100‑1 (MQ=255)
                        tggATTCATCATTACGCGCAGTCTGGTGAACTTAGACGCTTGGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcggattgtcc                                                                           <  2:2337428/98‑9 (MQ=25)
                         tGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGttt                                                                          <  1:1342200/100‑1 (MQ=255)
                           aTTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTa                                                                        <  1:1095889/100‑1 (MQ=255)
                             tcatcaTTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATc                                                                      >  2:268541/1‑100 (MQ=255)
                              catcatTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATcc                                                                     >  1:1320225/1‑100 (MQ=255)
                               atcatTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCt                                                                    >  1:1453914/1‑100 (MQ=255)
                                 catTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTg                                                                  <  2:1686193/100‑1 (MQ=255)
                                 catTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTg                                                                  <  2:950392/100‑1 (MQ=255)
                                   ttACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGaa                                                                <  1:900975/100‑1 (MQ=255)
                                     aCGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAgg                                                              <  2:2038596/100‑1 (MQ=255)
                                      cGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAGTCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGt                                                             >  1:855919/1‑100 (MQ=255)
                                        cccAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTg                                                           <  1:566906/100‑1 (MQ=255)
                                        cccAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTg                                                           <  1:1955515/100‑1 (MQ=255)
                                         ccAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGc                                                          >  1:1622638/1‑100 (MQ=255)
                                          cAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGcc                                                         <  2:43784/100‑1 (MQ=255)
                                          cAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGcc                                                         <  2:902657/100‑1 (MQ=255)
                                          cAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAAACCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGcc                                                         <  2:2316746/100‑1 (MQ=255)
                                             tCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGa                                                      <  2:581126/100‑1 (MQ=255)
                                                ggTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAAc                                                   <  1:1574582/100‑1 (MQ=255)
                                                ggTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAAc                                                   <  1:777698/100‑1 (MQ=255)
                                                 gTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACa                                                  >  1:353766/1‑100 (MQ=255)
                                                  tGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAg                                                 >  1:1207287/1‑100 (MQ=255)
                                                      cTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTg                                             >  1:35268/1‑100 (MQ=255)
                                                      cTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTg                                             <  1:951106/100‑1 (MQ=255)
                                                      cTTAGACGCTTAATGATCGAATGTCGGGTGGACAATCCGAAAAGCAACCAGGTCGCTTTGCGCAATGGGTTTATCCTTGAAGGGTGCCTGAAACAGGCt                                              >  2:2367872/1‑99 (MQ=255)
                                                      cTTAGACGCGTCGTGATCAAATGTCGGGGGGACAATCCGCAAACAAACCAGGTCGCTTTGCGCGATGGTTTTATTCTTGAAGGTTGACTGATACAGGCt                                              >  2:1165044/1‑99 (MQ=255)
                                                        tAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAg                                           <  2:875444/100‑1 (MQ=255)
                                                        tAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAg                                           <  2:1483947/100‑1 (MQ=255)
                                                           aCGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTc                                        <  1:804009/100‑1 (MQ=255)
                                                             gCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCt                                      <  1:217654/100‑1 (MQ=255)
                                                               ttCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGa                                    <  2:1202736/100‑1 (MQ=255)
                                                                tCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGaa                                   <  1:1243575/100‑1 (MQ=255)
                                                                 cGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAat                                  <  2:35267/100‑1 (MQ=255)
                                                                  gTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAatg                                 <  2:768401/100‑1 (MQ=255)
                                                                   tGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAatga                                >  2:967304/1‑100 (MQ=255)
                                                                   tGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGATTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCt                                      >  2:1119321/1‑94 (MQ=255)
                                                                     aTCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAatgatg                              <  1:1811806/100‑1 (MQ=255)
                                                                       cAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGcc                            <  1:670480/100‑1 (MQ=255)
                                                                       cAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGcc                            >  2:285406/1‑100 (MQ=255)
                                                                         aaTGTCGGGTGGACAATCCGCAAATCAACCAGGTCGCATTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTa                          <  2:552978/100‑1 (MQ=255)
                                                                          aTGTCGGGTGGACAATCCGCAAAGCAACCAGGGCGCTTGGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTat                         <  2:759015/100‑1 (MQ=255)
                                                                             tCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatgat                      <  1:709228/100‑1 (MQ=255)
                                                                             tCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatgat                      <  1:202242/100‑1 (MQ=255)
                                                                                   ggACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGAtgtg                >  2:694550/1‑100 (MQ=255)
                                                                                   ggACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGAtgtg                >  1:2160519/1‑100 (MQ=255)
                                                                                      cattgcGCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAAc             >  1:1171144/6‑100 (MQ=38)
                                                                                      cAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAAc             >  1:935846/1‑100 (MQ=255)
                                                                                       aaTCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACt            <  2:353764/100‑1 (MQ=255)
                                                                                        aTCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACtt           <  1:182663/100‑1 (MQ=255)
                                                                                          gcGCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtat         >  1:894415/2‑100 (MQ=39)
                                                                                          ccGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtat         >  1:1247247/1‑100 (MQ=255)
                                                                                           cGCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtata        >  1:590907/1‑100 (MQ=255)
                                                                                           cGCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtata        >  1:969662/1‑100 (MQ=255)
                                                                                           cGCAAAGCGACCTGGTTGCTTTGCGCAATGGATTTATCCTTGAAGGTTGCCTGAAACAGGCTGCGTTCCTGAATGATGCCTATGATGATGTGAACTtata        >  2:2269186/1‑100 (MQ=255)
                                                                                           cGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtata        <  1:967307/100‑1 (MQ=255)
                                                                                           cGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtata        >  1:1979321/1‑100 (MQ=255)
                                                                                            gCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAc       >  2:1921404/1‑100 (MQ=255)
                                                                                            gCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAc       >  1:1757716/1‑100 (MQ=255)
                                                                                             cAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAcg      >  2:627437/1‑100 (MQ=255)
                                                                                               aaGCAACCAGTTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAcgcg    <  2:1902743/100‑1 (MQ=255)
                                                                                                 gCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAcgcgcg  <  2:426655/100‑1 (MQ=255)
                                                                                                 gCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAcgcgcg  <  2:2120591/100‑1 (MQ=255)
                                                                                                   |                                                                                                 
ATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCG  >  NC_000913/1499262‑1499458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑