| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,554,731 | C→T | 100% | M313I (ATG→ATA) | maeA ← | malate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,554,731 | 0 | C | T | 93.1% | 220.7 / 5.0 | 76 | M313I (ATG→ATA) | maeA | malate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme |
| Reads supporting (aligned to +/- strand): ref base C (2/3); new base T (43/26); total (47/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CATCTTGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGT > NC_000913/1554635‑1554827 | cATCTTGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGattatt > 1:1864304/1‑100 (MQ=255)cATCTTGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGattatt < 2:1743397/100‑1 (MQ=255)cATCTTGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGATTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGattatt < 2:1743455/100‑1 (MQ=255) aTCTTGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTAttt > 1:1115534/1‑100 (MQ=255) aTCTTGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTAttt > 1:298494/1‑100 (MQ=255) tCTTGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTc > 1:2046880/1‑100 (MQ=255) tCTTGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTc < 1:7966/100‑1 (MQ=255) cTTGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGCTTATTTc > 2:2311584/1‑99 (MQ=255) cTTGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCg > 1:1349241/1‑100 (MQ=255) agCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATCTCGGCAATGCCGc > 1:630333/1‑100 (MQ=255) agCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGc > 1:65402/1‑100 (MQ=255) gCAAGCCAAAGCGGTCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCa > 2:1309511/1‑100 (MQ=255) gCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCa > 2:1024150/1‑100 (MQ=255) gCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCa > 2:599103/1‑100 (MQ=255) gCAAGCCAAAGCGATCGACCATAAAGACTTGCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCa < 1:1915869/100‑1 (MQ=255) aGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATcc > 2:1443003/1‑100 (MQ=255) aGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATcc > 1:1822200/1‑100 (MQ=255) gCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATccc > 1:890020/1‑100 (MQ=255) aaaGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCt > 1:393885/1‑100 (MQ=255) aaGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTg > 1:2040881/1‑100 (MQ=255) gCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGaa < 2:1864290/100‑1 (MQ=255) ngATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAAc > 1:452821/2‑100 (MQ=255) cGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAAc < 2:867586/100‑1 (MQ=255) cGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAAc > 1:453998/1‑100 (MQ=255) gATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGCTGATTATTTCGGCAATGCCGCATCCCGCTGAAcc > 2:2125776/1‑100 (MQ=255) cGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTgc > 2:237344/1‑100 (MQ=255) gACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTgcg < 1:2268416/100‑1 (MQ=255) ccATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGcc < 2:55987/100‑1 (MQ=255) cATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCa < 2:1338171/100‑1 (MQ=255) aTAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCaa > 1:48811/1‑100 (MQ=255) tAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAg > 1:1532625/1‑100 (MQ=255) aGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGaa > 1:2023691/1‑100 (MQ=255) aGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGaa < 2:733393/100‑1 (MQ=255) gACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAg < 2:1729059/100‑1 (MQ=255) aCTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGa > 2:1617965/1‑100 (MQ=255) cTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGAc > 2:1939075/1‑100 (MQ=255) cTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGAc < 1:793298/100‑1 (MQ=255) cTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGAc > 1:1089641/1‑100 (MQ=255) cTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGAc > 2:779300/1‑100 (MQ=255) tGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGAttt > 1:619238/1‑100 (MQ=255) gccgagccgcTTCCTCGCTTAATCCTTCGCGCTGGGACTGGGAGCTGCTTATTTCGGCCATGCCGCACCCCGCGGCATCTGCTCCCAGGaaacc > 2:1413627/1‑91 (MQ=255) ccgcgccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGAttttt < 1:47633/100‑1 (MQ=255) cgcgccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGAtttttt > 1:1203909/1‑100 (MQ=255) cgccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTtc < 1:1562877/100‑1 (MQ=255) cgccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTtc < 2:403426/100‑1 (MQ=255) ccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTtctc < 1:1945254/100‑1 (MQ=255) cTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCt < 1:154262/100‑1 (MQ=255) tCCTCTCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTa < 2:601920/100‑1 (MQ=255) ccTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGAAAATTTTTTTCCCGCTTa > 1:438281/1‑99 (MQ=255) cTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAAc > 2:618528/1‑100 (MQ=255) cTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAAc > 1:1199708/1‑100 (MQ=255) cGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGGCGATTTTTTTCTCGCTTAACTg < 2:1381453/100‑1 (MQ=255) cGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTg > 1:1289943/1‑100 (MQ=255) cGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTg > 1:1928175/1‑100 (MQ=255) cGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTg < 2:1699243/100‑1 (MQ=255) cGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTg > 1:1462429/1‑100 (MQ=255) tAATCCTTCGCGCTGGGTCTTGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGacc < 2:1589082/99‑1 (MQ=255) tAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGacca > 1:731200/1‑100 (MQ=255) ccTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGaccacc < 2:373644/98‑1 (MQ=255) cTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGc > 1:64550/1‑100 (MQ=255) cTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGc < 1:618530/100‑1 (MQ=255) cTTCGCGCTGGGTCTGGGAGATGATAACTTCGGCAATGCCGCATCCGACTGAACCTGCACCTCGGAAGACGGCTTTTTTCTCGcct > 2:721849/1‑84 (MQ=255) ttCGCTCTGGAACTAGGAGACGACAATTTCGGCAATGCCGCATCCCGCTGAACCTGCCCCAAGAAACACGATTTTTTTCTCGCTCAACTGACCACCTGcc > 2:244300/1‑100 (MQ=255) cgcTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCcg > 2:950344/1‑97 (MQ=255) cTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCgg < 1:2191202/100‑1 (MQ=255) cTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCgg < 2:65401/100‑1 (MQ=255) tGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGc < 1:628637/100‑1 (MQ=255) gTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTtg < 1:1482086/100‑1 (MQ=255) gTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTtg > 2:756357/1‑100 (MQ=255) tCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATATTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTtgc < 2:1463134/100‑1 (MQ=255) cTGGGAGCTGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTCTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTtgct < 2:1275839/100‑1 (MQ=255) cTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTtgct < 2:708268/100‑1 (MQ=255) gggAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTtgctgc > 2:152091/1‑100 (MQ=255) gagaTGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGa > 2:1860382/1‑100 (MQ=255) agaTGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGAt < 2:1893603/100‑1 (MQ=255) tgatTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATc > 2:1653471/1‑98 (MQ=255) tgatTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAg > 2:1777873/1‑100 (MQ=255) tgatTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAg > 1:1229358/1‑100 (MQ=255) gatTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAgt > 1:1877735/1‑100 (MQ=255) | CATCTTGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGT > NC_000913/1554635‑1554827 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |