Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 83,590 G→T 16.2% intergenic (‑61/+32) leuA ← / ← leuL 2‑isopropylmalate synthase/leu operon leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091383,5900GT16.2% 277.1 / 47.3 112intergenic (‑61/+32)leuA/leuL2‑isopropylmalate synthase/leu operon leader peptide
Reads supporting (aligned to +/- strand):  ref base G (39/55);  new base T (8/10);  total (47/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.15e-02

Only 100 of 112 total aligned reads displayed.

GCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATA  >  NC_000913/83493‑83688
                                                                                                 |                                                                                                   
gcAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGgc                                                                                                   >  1:663076/1‑100 (MQ=255)
   aTGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGcgcg                                                                                                <  1:1406464/100‑1 (MQ=255)
    tgtgGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCgg                                                                                               <  2:893235/100‑1 (MQ=255)
     gtgtTATCGAAAATAATTACTTGCTGGCTCATGGTTTGGGTCCTTGTATATTTTAAAACGAATAAATTAAAAAATAAAAAAACCCGCGCAATGGCGCggg                                                                                              <  2:151362/100‑1 (MQ=255)
     gtgGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCggg                                                                                              <  2:237640/100‑1 (MQ=255)
     gtgGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCggg                                                                                              >  2:373134/1‑100 (MQ=255)
      tgGTATCTAAAATAATGACTTGCTGGCACATGGTTTGGGTCCTTGTCTCTTTTCGAGCGCCGCGCTTCGGGCATAAAAAACCCGCGCCATTGCGCGGGtt                                                                                           <  2:2179904/100‑1 (MQ=37)
      tgGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGt                                                                                            <  1:1299849/100‑1 (MQ=255)
      tgGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGt                                                                                            <  1:591622/100‑1 (MQ=255)
      tgGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGCCGCGGGt                                                                                            <  1:1253410/100‑1 (MQ=255)
       ggTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGtt                                                                                           <  1:1435665/100‑1 (MQ=255)
       ggTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGtt                                                                                           <  2:891492/100‑1 (MQ=255)
          aTCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGttttt                                                                                        <  1:514183/100‑1 (MQ=255)
          aTCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGttttt                                                                                        >  1:2080806/1‑100 (MQ=255)
          aTCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGttttt                                                                                        >  1:268519/1‑100 (MQ=255)
           tCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGtttttt                                                                                       <  1:291156/100‑1 (MQ=255)
            cGAAAATAATGACTTGCTGGCTCATTGTTTGGGTCCTTGTTTCTTTTAGAGCGCCTCTCTTCGGGCATAAAAAAAGCCGCGCAATGGCGCGGGTTCtttg                                                                                      >  1:495195/1‑100 (MQ=255)
            cGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttg                                                                                      <  1:947158/100‑1 (MQ=255)
            cGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTGTtttg                                                                                      >  2:701172/1‑100 (MQ=255)
            cGAAAATAATGACTTGCTGACTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCTGGCATAAAAAAACCCGCGCAATGGAGCCGGttttt                                                                                        >  2:1153892/1‑98 (MQ=255)
             gAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGttttttta                                                                                     <  1:1768021/100‑3 (MQ=255)
             gAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCACTGGCGCGGGTTTtttgt                                                                                     >  1:22381/1‑100 (MQ=255)
              aaaaTAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgtt                                                                                    <  1:1798226/100‑1 (MQ=255)
              aaaaTAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTCTtttgtt                                                                                    <  1:1252430/100‑1 (MQ=255)
              aaaaTAATGACGTGCTGGGTCATGGGTTGGGTCCTTGTCTCTTTTAGAGCGCGTCGCTTTTGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgtt                                                                                    <  2:1558376/100‑1 (MQ=255)
               aaaTAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTATAGCGCCTCGCTTCGGGCATAAAACAACCCGCGCAATGGCCCGGGTTTtttgttt                                                                                   <  2:487394/100‑1 (MQ=255)
                aataatGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgtttg                                                                                  <  2:1856683/100‑1 (MQ=255)
                 ataatGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGtttttttatgcc                                                                                 <  2:312888/100‑7 (MQ=37)
                  taatGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGAc                                                                                <  2:1708201/100‑1 (MQ=255)
                   aatGACTTGCTGGCTCATGGTTTGAGTCCTTGTCCCTTTTAGAGCGCACCGCTTCGTGCATCAACAAACCCGCGCAATGACGCGGGTTTCTTGTTCGAc                                                                                <  2:348982/99‑1 (MQ=255)
                     tGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGAc                                                                                <  2:1189803/97‑1 (MQ=255)
                     tGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTg                                                                              <  2:1685228/99‑1 (MQ=255)
                      gACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCg                                                                            <  2:420992/100‑1 (MQ=255)
                            cTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTgg                                                                      <  2:1311937/100‑1 (MQ=255)
                             tGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGc                                                                     <  2:1747278/100‑1 (MQ=255)
                             tGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGCGCTgga                                                                     <  1:908063/100‑2 (MQ=255)
                              ggCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCt                                                                    >  2:2089024/1‑100 (MQ=255)
                                cTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTa                                                                  >  1:886539/1‑100 (MQ=255)
                                cTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTa                                                                  >  1:2273978/1‑100 (MQ=255)
                                cTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTa                                                                  <  2:1584838/100‑1 (MQ=255)
                                cTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTa                                                                  >  2:1106315/1‑100 (MQ=255)
                                cTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTa                                                                  >  1:698159/1‑100 (MQ=255)
                                cTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTa                                                                  >  2:1410614/1‑100 (MQ=255)
                                  cATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAAt                                                                >  1:580730/1‑100 (MQ=255)
                                  cATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAAt                                                                <  2:1318555/100‑1 (MQ=255)
                                    tGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGc                                                              >  1:589978/1‑100 (MQ=255)
                                    tGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGc                                                              >  1:452669/1‑100 (MQ=255)
                                    tGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGc                                                              <  2:1798271/100‑1 (MQ=255)
                                     ggTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTACTGGCTTAATGCt                                                             >  1:1386226/1‑100 (MQ=255)
                                        ttGGGTCCTTGTCTCCTTTAGAGCGACTTGCTTTCGTCAGAAAAAAGCCCGCGCAATGGCGGGGGTTTTTTGTTTGATGGCGTGCTGGCTTAATGCTg                                                            <  2:279135/98‑1 (MQ=255)
                                            gTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGcc                                                      >  1:415404/1‑100 (MQ=255)
                                             tCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCAGATTTTTTGTTTAACAGCGTGCTGGCTTAATGCTGGATGACg                                                     <  2:943888/100‑1 (MQ=255)
                                              ccTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGc                                                    <  2:1200042/100‑1 (MQ=255)
                                               cTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCt                                                   >  2:1128518/1‑100 (MQ=255)
                                                ttGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTc                                                  <  1:304729/100‑1 (MQ=255)
                                                ttGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTc                                                  <  1:657932/100‑1 (MQ=255)
                                                  gTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCAc                                                <  1:701174/100‑1 (MQ=255)
                                                    ctctTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTc                                              <  1:775684/100‑1 (MQ=255)
                                                     tctTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCg                                             <  2:1358418/100‑1 (MQ=255)
                                                      ctTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGt                                            >  2:1179137/1‑100 (MQ=255)
                                                         tgagagCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTa                                         >  1:1614744/3‑100 (MQ=255)
                                                          tAGAGCGCGTCGCTTCGGGCATAAAAATTCCCGCGCTATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCt                                          <  2:2053468/98‑1 (MQ=255)
                                                          tAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTAc                                        >  1:2175756/1‑100 (MQ=255)
                                                            gagCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTAcc                                       >  2:610665/1‑99 (MQ=255)
                                                              gcgcCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACcgcg                                    >  1:2099340/1‑100 (MQ=255)
                                                                gcCTCGCTTCGGGCATAAAAAATCCCGCGCAATGTCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCa                                  <  2:1830740/100‑1 (MQ=255)
                                                                gcCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCa                                  <  2:2307151/100‑1 (MQ=255)
                                                                gcCTCGCTTCGGGCATAAAAAAACCCGCGCAACGGCCCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCa                                  <  1:269934/100‑1 (MQ=255)
                                                                 ccTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCaa                                 <  1:2089041/100‑1 (MQ=255)
                                                                  cTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCaaa                                >  1:1465040/1‑100 (MQ=255)
                                                                     gCTTCGGGCATAAACACGCCCGCGCAATGGCGCGGGTTTGTTGTTTGACTGCGGGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAaaga                              <  2:1366180/99‑1 (MQ=255)
                                                                     gCTTCGGGCATAAACAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAaagaa                             >  1:715946/1‑100 (MQ=255)
                                                                     gCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAaagaa                             >  1:1426624/1‑100 (MQ=255)
                                                                         cgatcttAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATg                         <  2:1903521/94‑1 (MQ=255)
                                                                          gggCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAaagaag                            <  2:568311/96‑1 (MQ=255)
                                                                             aaacaaaaaaCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGtt                     >  2:2249510/5‑100 (MQ=38)
                                                                              aacaaaaaaCCCGCGCCATTGCGCGGGTTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCACAGAAGATGCGt                      >  1:1632433/4‑99 (MQ=37)
                                                                              aTAAAAAAACCCGCGCTATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGttt                    <  1:1028369/100‑1 (MQ=255)
                                                                              aTAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGttt                    <  1:1973142/100‑1 (MQ=255)
                                                                               tAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATgagatcg                   >  1:1903537/1‑94 (MQ=255)
                                                                               tAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTta                   >  1:2248337/1‑100 (MQ=255)
                                                                               acaaaaaaCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTta                   >  1:545486/3‑100 (MQ=255)
                                                                                caaaaaaCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCCAATAAAATGCGTTtag                  >  1:2102888/2‑100 (MQ=38)
                                                                                caaaaaaCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtag                  >  2:2081841/2‑100 (MQ=255)
                                                                                caaaaaaCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtag                  >  2:1516115/2‑100 (MQ=255)
                                                                                aaaaaaaCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtag                  >  1:1787660/1‑100 (MQ=255)
                                                                                aaaaaaaCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtag                  >  2:1096987/1‑100 (MQ=255)
                                                                                 aaaaaaCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagt                 >  1:889026/1‑100 (MQ=255)
                                                                                 aaaaaaCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagt                 <  1:400527/100‑1 (MQ=255)
                                                                                   aaaaCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtag               <  2:874938/100‑1 (MQ=255)
                                                                                     aaCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagta             <  1:306438/100‑1 (MQ=255)
                                                                                       cccGCGCCATTGCGCGGGTTTTTTGTTTGACTCCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtagt           >  1:758549/1‑100 (MQ=255)
                                                                                       cccGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtagt           >  1:1676187/1‑100 (MQ=255)
                                                                                       cccGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGACTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtag            <  2:750955/99‑1 (MQ=255)
                                                                                             cAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCg     >  1:932696/1‑100 (MQ=255)
                                                                                             cAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCg     <  2:304798/100‑1 (MQ=255)
                                                                                              aaTGGCGCGGGTTTTTTTTTTTTTTTTGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTCGTAGTAGTAGACCGa    >  2:2070141/1‑100 (MQ=255)
                                                                                               aTGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGAt   <  2:332634/100‑1 (MQ=255)
                                                                                                tGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGa  <  2:22380/99‑2 (MQ=255)
                                                                                                tGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATa  <  1:431963/100‑1 (MQ=255)
                                                                                                 |                                                                                                   
GCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATA  >  NC_000913/83493‑83688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: