| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,868,250 | A→G | 100% | Y448C (TAC→TGC) | yeaG → | protein kinase, endogenous substrate unidentified; autokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,868,250 | 0 | A | G | 96.4% | 222.6 / ‑3.3 | 68 | Y448C (TAC→TGC) | yeaG | protein kinase, endogenous substrate unidentified; autokinase |
| Reads supporting (aligned to +/- strand): ref base A (2/1); new base G (31/34); total (33/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTACGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATG > NC_000913/1868153‑1868345 | aTGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTGGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCg > 2:1526067/1‑100 (MQ=255)aTGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCg < 1:33947/100‑1 (MQ=255) gAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTc > 1:546986/1‑100 (MQ=255) ggTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTgg < 2:326388/100‑1 (MQ=255) gTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAACAAACCCGGTCCATCTGTTCTGCGTCCTGGa > 1:1745534/1‑100 (MQ=255) cTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAAc > 2:2048728/1‑100 (MQ=255) cTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAAc > 2:163026/1‑100 (MQ=255) cTGTCGACGCGTTTTGCGTTTAAGATCCTCCCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAAc < 2:391404/100‑1 (MQ=255) gTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGCTCCATCTGTTCTGCGTCCTGGAAcag < 1:1447026/100‑1 (MQ=255) gTCGACGAGTTTTGCGTTTACGATCATCTCGCGCGTGTTCAACTTCGATCCTGTAGAAGTGGCAGCGCACCCGGTCCATCTGTTCTGCGTCCCGGAAcag > 2:1011298/1‑100 (MQ=255) gACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAAcagcag > 2:2017373/1‑100 (MQ=255) cgTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTg < 2:449394/100‑1 (MQ=255) cgTTTTGCGTGTAAGATCCTCTCCCTCGTGTTCAACTTCTATCATGTAGAAGTTGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGAt < 2:1272558/98‑1 (MQ=255) tttGCGTTTAAGATCCTCTCCCGCGTGTTTAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCTATCTGTTCTGCGTCCTGGAACAGCAGATTGAgn < 2:1430838/100‑2 (MQ=255) tttGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAgc > 1:1430846/1‑100 (MQ=255) gCGTTGAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGcgcg < 2:2208540/100‑1 (MQ=255) cGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGa > 1:1254951/1‑100 (MQ=255) gcttAAGATCCTCTCCCGCGCGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTACGTCCTGGAACAGCAGATTGAGCGCCAg > 2:237914/3‑100 (MQ=255) gTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAg > 1:1222925/1‑100 (MQ=255) gTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAg > 2:639864/1‑100 (MQ=255) tttAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGc < 2:1121956/100‑1 (MQ=255) ttAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCa < 1:653165/100‑1 (MQ=255) tAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAg > 2:907033/1‑100 (MQ=255) aaGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGt < 1:602936/100‑1 (MQ=255) ttctctCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTcc < 2:765116/97‑1 (MQ=255) tCCTCTCCCGCGTGTTCAACTTGGATCATGTGGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTccc < 1:1154208/100‑1 (MQ=255) tCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTccc < 2:601473/100‑1 (MQ=255) ccTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGGAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCca < 2:1301246/100‑1 (MQ=255) ccTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCca > 2:7890/1‑100 (MQ=255) ccTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCca < 1:730243/100‑1 (MQ=255) ccTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTACGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTccc < 2:2086311/99‑1 (MQ=255) ctctCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCcac < 1:1790480/100‑1 (MQ=255) ctctCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCcac < 2:843992/100‑1 (MQ=255) tctcCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTACGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCca > 1:1483880/1‑98 (MQ=255) tcCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAag < 1:1038990/100‑1 (MQ=255) tcCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAag < 1:1951050/100‑1 (MQ=255) cccGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAaga > 2:311085/1‑100 (MQ=255) gcgTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAgca < 1:293363/100‑1 (MQ=255) cgTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAgcag < 1:1698767/100‑1 (MQ=255) gtgtTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAgcagg < 1:402435/100‑1 (MQ=255) tCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGa < 1:2303868/100‑1 (MQ=255) aaCTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAAc > 1:654699/1‑100 (MQ=255) aCTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACg < 2:552787/100‑1 (MQ=255) cTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGc > 1:351540/1‑100 (MQ=255) cTTCGATCATGTAGAAGTGGAAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGAATAAGCGCGAGCAGTTCCCACAAGAGCAGGCAGaa > 2:1866015/1‑97 (MQ=255) aTCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCt > 1:2038189/1‑100 (MQ=255) tCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTg > 2:2016723/1‑100 (MQ=255) aTGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGa < 2:1750727/100‑1 (MQ=255) aTGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGa > 1:1193523/1‑100 (MQ=255) tGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAg > 1:793796/1‑100 (MQ=255) aaGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCt < 1:1825398/100‑1 (MQ=255) ggCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGaaa > 1:1800385/1‑100 (MQ=255) ggCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGaaa > 2:589678/1‑100 (MQ=255) cagcaAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAgg > 2:1939235/1‑100 (MQ=255) cagcaAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAgg > 1:1003702/1‑100 (MQ=255) agcaAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGACTTCCTGAAGGGt > 1:610470/1‑100 (MQ=255) caAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTa < 1:915472/100‑1 (MQ=255) caAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTa < 2:793794/100‑1 (MQ=255) aCCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGCGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCt > 2:1027864/1‑100 (MQ=255) aCCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCt < 1:615934/100‑1 (MQ=255) cccGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTg < 1:2017391/100‑1 (MQ=255) ggTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATc < 2:662785/100‑1 (MQ=255) ccATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCATAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCg > 2:1301765/1‑100 (MQ=255) ccATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCg > 2:1634398/1‑100 (MQ=255) tgtGTTCTGCGTCCTGGAACAGCAGATGGAGCGCGAGCAGTTTTCACAAGTGCAGGCAGCATGCCATCTGGAATTCTTGAAAGGTTATCTGATCCCGa < 2:1972097/96‑1 (MQ=255) tCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGaaa > 1:1188409/1‑100 (MQ=255) cTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAat > 1:254245/1‑100 (MQ=255) ttCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATg < 1:311087/100‑1 (MQ=255) | ATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTACGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATG > NC_000913/1868153‑1868345 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |