Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,753,986 A→T 15.1% intergenic (+40/+22) bamE → / ← ratB lipoprotein component of BamABCDE OM biogenesis complex/UPF0125 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,753,9860AT15.1% 276.4 / 39.3 106intergenic (+40/+22)bamE/ratBlipoprotein component of BamABCDE OM biogenesis complex/UPF0125 family protein
Reads supporting (aligned to +/- strand):  ref base A (46/44);  new base T (9/7);  total (55/51)
Fisher's exact test for biased strand distribution p-value = 7.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01

Only 100 of 107 total aligned reads displayed.

TTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCC  >  NC_000913/2753887‑2754083
                                                                                                   |                                                                                                 
tttAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCaa                                                                                                   <  2:55598/100‑1 (MQ=255)
 ttAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAAt                                                                                                  >  2:1450471/1‑100 (MQ=255)
  tAACAGTAGCGGTGTGTTAACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCATTg                                                                                                 <  2:1081069/100‑1 (MQ=255)
   aaCAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGa                                                                                                >  1:542988/1‑100 (MQ=255)
    aCAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAg                                                                                               >  2:1773663/1‑100 (MQ=255)
     cAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGc                                                                                              <  1:2111509/100‑1 (MQ=255)
      aGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCa                                                                                             >  2:631842/1‑100 (MQ=255)
        tAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCAcc                                                                                           <  1:1797757/100‑1 (MQ=255)
         aGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTCATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCt                                                                                          >  1:1269370/1‑100 (MQ=255)
            ggTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCATTGAGCACCtttt                                                                                       <  1:929313/100‑1 (MQ=255)
            ggTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCtttt                                                                                       <  2:700519/100‑1 (MQ=255)
            ggTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCtttt                                                                                       >  1:1931152/1‑100 (MQ=255)
             gtgtgtTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCATTGAGCACCttttt                                                                                      <  1:1772643/100‑1 (MQ=255)
               gtgtTGACCAATATTGATGACAAACCTGCGCTGAGTGATAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCATTGAGCACCtttttta                                                                                    <  1:1964433/100‑2 (MQ=38)
               gtgtTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtc                                                                                    <  1:877209/100‑1 (MQ=255)
               gtgtTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtc                                                                                    <  2:1887491/100‑1 (MQ=255)
               gtgtTGACCAATATTGATAACAAAACTGCGCTGAGTGGTAACTAATATTAGAGTTGCTCTTAAAGACGTTAAAAAAGGTGCTCATTGAGCACCtttttta                                                                                    <  2:2222962/100‑2 (MQ=38)
                tgtTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAATACGTTAAAAAAGGTGCTCATTGAGCACCttttttaa                                                                                   <  1:579079/100‑3 (MQ=255)
                tgtTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCATTGAGCACCttttttaa                                                                                   <  2:894920/100‑3 (MQ=255)
                 gtTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtctg                                                                                  >  2:295017/1‑100 (MQ=255)
                 gtTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtctg                                                                                  >  2:775990/1‑100 (MQ=255)
                 gtTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtctg                                                                                  >  1:2121781/1‑100 (MQ=255)
                 gtTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtctg                                                                                  >  2:1806883/1‑100 (MQ=255)
                  ttGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtctgt                                                                                 <  1:1519198/100‑1 (MQ=255)
                   tGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtctgtc                                                                                <  2:400644/100‑1 (MQ=255)
                   tGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtctgtc                                                                                >  2:2014531/1‑100 (MQ=255)
                    gACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtctgtct                                                                               >  1:909720/1‑100 (MQ=255)
                     aCCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtctgtctg                                                                              >  1:1084704/1‑100 (MQ=255)
                     aCCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtctgtctg                                                                              <  1:2118563/100‑1 (MQ=255)
                      ccAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTctgtctgt                                                                             >  2:88625/1‑100 (MQ=255)
                      ccAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTctgtctgt                                                                             >  2:236606/1‑100 (MQ=255)
                      ccAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTctgtctgt                                                                             >  2:1221727/1‑100 (MQ=255)
                      ccAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTctgtctgt                                                                             >  2:2343577/1‑100 (MQ=255)
                          tatTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGttatt                                                                         >  1:746144/1‑100 (MQ=255)
                           atTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGttattt                                                                        >  1:1352928/1‑100 (MQ=255)
                            ttGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTACAAAAGGTGCTCAATGAGCACCTTTTTTCTGTATGttattta                                                                       >  2:820482/1‑100 (MQ=255)
                             tGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGttatttat                                                                      <  1:334320/100‑1 (MQ=255)
                              gATAACAAACCTGCGCTGAGTTGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTtatttatt                                                                     <  2:1721890/100‑1 (MQ=255)
                               aTAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTc                                                                    <  2:2262855/100‑1 (MQ=255)
                               aTAACAAACCTGCGCTGAGTGGTAACTAATAACAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTc                                                                    <  2:1770540/100‑1 (MQ=255)
                                tcaCAAACCTGCGCTCAATCGTAACTAGTAATAAACCTCCTCTCACCCACGTTAAACAAGGTGCTTAATGAGCACCGTTTTTCTGTCTGttat                                                                          <  2:1955892/91‑1 (MQ=255)
                                tAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCg                                                                   <  1:255344/100‑1 (MQ=255)
                                tAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCg                                                                   >  1:1963753/1‑100 (MQ=255)
                                 aaCAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGc                                                                  >  1:87493/1‑100 (MQ=255)
                                 aaCAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGc                                                                  >  1:2018633/1‑100 (MQ=255)
                                 aaCAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTATGTtgtttatt                                                                     >  2:1953303/1‑97 (MQ=255)
                                  aCAGACCTGCGCTGAGTGGTAACTAATAATTTATTTGCGCTCAAAGAGGTTAAAAAAGGTGCTCAATGAGCTCCTTTTTTCTGTCTGTTATTTATTCg                                                                   <  2:1550217/98‑1 (MQ=255)
                                  aCAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCt                                                                 >  1:1583536/1‑100 (MQ=255)
                                  aCAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCt                                                                 <  2:1925564/100‑1 (MQ=255)
                                  aCAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCt                                                                 >  1:2047622/1‑100 (MQ=255)
                                   cAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATAAGCACCTTTTTTCTGTCTGTTATTTATTCGc                                                                  >  2:2253101/1‑98 (MQ=255)
                                   cAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCCCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTCTTCTGTCTGCTATTTATTCGCTg                                                                >  2:1117432/1‑100 (MQ=255)
                                     aaCCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGAt                                                              <  1:1815067/100‑1 (MQ=255)
                                      aCCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGAtt                                                             <  1:1324684/100‑1 (MQ=255)
                                          gcgcTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTc                                                         <  1:631844/100‑1 (MQ=255)
                                             cTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGGGCTCCTTTTATCTGTCTGTTATTTATTCGCTGATTTTTCTGc                                                      >  2:1671419/1‑100 (MQ=255)
                                             cTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGc                                                      >  1:592079/1‑100 (MQ=255)
                                               gAGTGGTAACTAATAATAAAGTTGATCTCAAAGACGTTAAATAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTg                                                       <  2:1973542/97‑1 (MQ=255)
                                                aGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCg                                                   <  2:87492/100‑1 (MQ=255)
                                                   ggTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTg                                                >  1:872485/1‑100 (MQ=255)
                                                     tAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGcc                                              <  1:1039441/100‑1 (MQ=255)
                                                     tAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGcc                                              <  1:1115626/100‑1 (MQ=255)
                                                     tAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGcc                                              <  2:1037860/100‑1 (MQ=255)
                                                     tAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGcc                                              <  1:2247365/100‑1 (MQ=255)
                                                          aataataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGa                                         >  1:912167/1‑100 (MQ=255)
                                                                 aaGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCtt                                  >  2:656050/1‑100 (MQ=255)
                                                                  aGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCttt                                 <  1:654349/100‑1 (MQ=255)
                                                                    ttGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCg                               <  2:1258727/100‑1 (MQ=255)
                                                                      gCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCCAAGCTCTTTCGGa                             <  1:1617679/100‑1 (MQ=255)
                                                                        tctcAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATc                           >  1:218450/1‑100 (MQ=255)
                                                                        tctcAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATc                           <  2:218449/100‑1 (MQ=255)
                                                                         ctcAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATc                           >  2:1498000/1‑99 (MQ=255)
                                                                         ctcAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCg                          <  1:1988222/100‑1 (MQ=255)
                                                                           cAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGTCTGCGAAGCTCTTTCGGATTgg                         >  2:2001481/1‑99 (MQ=255)
                                                                            aacagacagaaaaaaGGTGCTCATTGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCa                       >  2:43344/10‑100 (MQ=37)
                                                                            aaaGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCa                       >  2:254283/1‑100 (MQ=255)
                                                                             aaGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCaa                      >  1:1186910/1‑100 (MQ=255)
                                                                               gACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATg                    <  2:597975/100‑1 (MQ=255)
                                                                               gACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATg                    <  2:1711798/100‑1 (MQ=255)
                                                                                  gTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATgaga                 >  1:1296018/1‑100 (MQ=255)
                                                                                   ttAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGagag                <  2:2316252/100‑1 (MQ=255)
                                                                                   ttAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGagag                <  2:1497638/100‑1 (MQ=255)
                                                                                    gaaaaaaGGTGCTCATTGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAgg               >  1:2149592/2‑100 (MQ=255)
                                                                                    gaaaaaaGGTGCTCATTGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAgg               >  1:152664/2‑100 (MQ=255)
                                                                                     aaaaaaGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAggg              >  1:276255/1‑100 (MQ=255)
                                                                                      aaaaaGGTGCTCATTGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGc             >  2:2380570/1‑100 (MQ=255)
                                                                                      aaaaaGGTGCTCATTGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGc             >  1:264359/1‑100 (MQ=255)
                                                                                         aaGGTGCTCATTGAGCACCTTTTTTCGGTCGGTTATGTATTCGGTGATTTTTCTGGTCGTTGCCTGCAAGGCTCTTTCGGATCGGCAATGAGAGGGCGAt          >  1:1185673/1‑100 (MQ=255)
                                                                                         aaGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGAt          <  1:1827171/100‑1 (MQ=255)
                                                                                            gTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATaaa       <  1:254285/100‑1 (MQ=255)
                                                                                              gCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAAtc     <  2:909718/100‑1 (MQ=255)
                                                                                              gCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAAtc     <  1:656052/100‑1 (MQ=255)
                                                                                               cTCAATGATCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAAtc     <  1:1633282/99‑1 (MQ=255)
                                                                                               cTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAAtc     <  2:2199447/99‑1 (MQ=255)
                                                                                                tCATTGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGTTAAAtctc   >  2:1848764/1‑100 (MQ=255)
                                                                                                tCATTGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAAtctc   >  2:651189/1‑100 (MQ=255)
                                                                                                tCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAAtctc   <  2:592077/100‑1 (MQ=255)
                                                                                                tCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAAtctc   >  1:1063968/1‑100 (MQ=255)
                                                                                                 cAATGAGTACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTcc  >  2:30332/1‑100 (MQ=255)
                                                                                                 cAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTcc  >  2:785171/1‑100 (MQ=255)
                                                                                                   |                                                                                                 
TTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCC  >  NC_000913/2753887‑2754083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: