Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,814,175 T→A 15.6% intergenic (+21/+43) emrB → / ← luxS multidrug efflux system protein/S‑ribosylhomocysteine lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,814,1750TA15.6% 260.0 / 33.2 101intergenic (+21/+43)emrB/luxSmultidrug efflux system protein/S‑ribosylhomocysteine lyase
Reads supporting (aligned to +/- strand):  ref base T (38/46);  new base A (5/11);  total (43/58)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

TTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTG  >  NC_000913/2814077‑2814272
                                                                                                  |                                                                                                 
ttCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAttt                                                                                                  <  1:1196881/100‑1 (MQ=255)
 tCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTg                                                                                                 >  2:1112525/1‑100 (MQ=255)
 tCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTg                                                                                                 <  2:570466/100‑1 (MQ=255)
  ccTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGa                                                                                                >  1:1921697/1‑100 (MQ=255)
   cTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTg                                                                                                 >  2:400691/1‑98 (MQ=255)
   cTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGaa                                                                                               >  1:292273/1‑100 (MQ=255)
      ggcctgggggggGTGGTGTGGGGTGGTGAGCCGGCATTTGGTGCAGGTGGCGGCGGTGGCGGTGCGCACTTAGTACTAGTAAGCCAGTTCATTTg                                                                                                 <  2:2081599/87‑1 (MQ=255)
      gTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACt                                                                                             >  1:256184/1‑99 (MQ=255)
       tCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAAc                                                                                              <  2:45551/97‑1 (MQ=255)
        ccggctgGGGCTGGTGTGGGGTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTgg                                                                                           <  1:1632269/96‑1 (MQ=255)
        ccTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGc                                                                                          >  1:339125/1‑100 (MQ=255)
         ctgctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGTGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCt                                                                                         <  2:1203017/100‑1 (MQ=255)
         ctgctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCt                                                                                         <  2:926774/100‑1 (MQ=255)
         ctgctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCt                                                                                         >  1:168056/1‑100 (MQ=255)
         ctgctgGGGCTGGTGTGGTTGGCTAAACCGACATTTGGGGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCt                                                                                         <  2:492772/100‑1 (MQ=255)
          tgctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCtt                                                                                        >  2:2131662/1‑100 (MQ=255)
          tgctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCtt                                                                                        <  1:1040873/100‑1 (MQ=255)
           gctgTGGCTGGTGTGGTGTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACCAAGCCAGTGCGAATGCACTGGCtca                                                                                       <  2:192202/100‑3 (MQ=255)
           gctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCtt                                                                                        <  1:904819/99‑1 (MQ=255)
            ctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCtttt                                                                                      >  2:1406102/1‑100 (MQ=255)
            ctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCttag                                                                                      <  2:425171/100‑3 (MQ=255)
             tgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCttagt                                                                                     <  1:834978/100‑4 (MQ=255)
             tgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCttag                                                                                      <  2:1002008/99‑3 (MQ=255)
              ggggCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCtttttt                                                                                    >  1:2014360/1‑100 (MQ=255)
              ggggCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCtttttt                                                                                    >  1:1238056/1‑100 (MQ=255)
              ggggCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCtttttt                                                                                    <  2:1542493/100‑1 (MQ=255)
              ggggCTGGTGTGCTGTGCTAAACCGCCATTTGGCGCTGGGGGCGGCTGAGGCGGTGCGTACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCttagtt                                                                                    <  2:589006/100‑5 (MQ=25)
               gggCTGGTGTGGTTTGCTAAACCTCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCttag                                                                                      <  2:1372486/97‑3 (MQ=39)
               gggCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTc                                                                                   <  2:52634/100‑1 (MQ=255)
                ggCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCa                                                                                  >  2:1224065/1‑100 (MQ=255)
                ggCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCttagttgt                                                                                  <  1:2052682/100‑7 (MQ=38)
                 gCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCaa                                                                                 >  2:854966/1‑100 (MQ=255)
                    ggTGTGTTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGCTGCGCATTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTc                                                                                   <  2:1450964/95‑1 (MQ=255)
                      tgtgGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCaattaat                                                                            <  1:2166920/100‑1 (MQ=255)
                       gtgGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCaattaatt                                                                           <  2:355006/100‑1 (MQ=255)
                         ggTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATtgt                                                                         >  2:1492398/1‑100 (MQ=255)
                           tttGCTAAACCGCCATTTGGCGCAGGTGGCGTCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGa                                                                       <  1:1132625/100‑1 (MQ=255)
                            ttGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGaa                                                                      >  1:63014/1‑100 (MQ=255)
                             tGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAg                                                                     >  2:1790000/1‑100 (MQ=255)
                             tGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAg                                                                     >  2:985324/1‑100 (MQ=255)
                              gCTAAACGGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTGATTTGAACTGGCTTTTTTCAATTAATTGTGAAGa                                                                    >  2:1254595/1‑100 (MQ=255)
                              gCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGa                                                                    <  2:699210/100‑1 (MQ=255)
                               cTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGAt                                                                   >  1:507234/1‑100 (MQ=255)
                               cTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGAt                                                                   >  1:1050310/1‑100 (MQ=255)
                                tAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATa                                                                  >  1:1045992/1‑100 (MQ=255)
                                     cGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTa                                                             >  1:80820/1‑100 (MQ=255)
                                      gCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTGCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTAc                                                            >  2:413989/1‑100 (MQ=255)
                                        cATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCAAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTg                                                          <  2:365438/100‑1 (MQ=255)
                                             ggCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAg                                                     <  2:2363748/100‑1 (MQ=255)
                                                gcAGGTGGCGGCGGAGGCGGTGCGCATTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGAtg                                                  <  2:453343/100‑1 (MQ=255)
                                                  aGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGAtgtg                                                <  2:913443/100‑1 (MQ=255)
                                                  aGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGAtgtg                                                <  2:161540/100‑1 (MQ=255)
                                                   ggTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGc                                               <  2:1989028/100‑1 (MQ=255)
                                                   ggTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGc                                               <  2:622337/100‑1 (MQ=255)
                                                   ggTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGc                                               <  1:1590262/100‑1 (MQ=255)
                                                   ggTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGc                                               <  1:1406505/100‑1 (MQ=255)
                                                    gTGGCGGCGGAGGCGGTGCGCACTAAGTACAATTAAGCCAGTTCATTTGTACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCa                                              <  2:1016969/100‑1 (MQ=255)
                                                    gTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGTCTTTTTTCAATTAATTGTGAACATAGTTTACTAACTAAATTTGc                                               >  1:2288111/1‑99 (MQ=255)
                                                     tGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAg                                             <  2:644893/100‑1 (MQ=255)
                                                       gcggcgGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGtt                                           <  2:2191222/100‑1 (MQ=255)
                                                         ggcggGTGCGGTGCGCACTAAGTACAATTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTcc                                         <  2:333676/100‑1 (MQ=255)
                                                         ggcggAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTcc                                         <  1:1840481/100‑1 (MQ=255)
                                                           cggAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTg                                       <  1:1156038/100‑1 (MQ=255)
                                                            ggAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGc                                      <  1:1964335/100‑1 (MQ=255)
                                                            ggAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGc                                      >  1:1282785/1‑100 (MQ=255)
                                                             gAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCa                                     <  1:766411/100‑1 (MQ=255)
                                                               ggCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAAc                                   <  1:1111303/100‑1 (MQ=255)
                                                                gCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACt                                  <  1:1503217/100‑1 (MQ=255)
                                                                  ggTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTtc                                <  1:848818/100‑1 (MQ=255)
                                                                  ggTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTtc                                >  1:1187997/1‑100 (MQ=255)
                                                                   gTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTtct                               <  1:583898/100‑1 (MQ=255)
                                                                    tGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTtctc                              <  1:392259/100‑1 (MQ=255)
                                                                     gcgcACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTctct                             >  1:918258/1‑100 (MQ=255)
                                                                        cACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAAGTGTGAAGATAGTTTACTGACTAGATGTGCAGTTTCTACAACTTCTCTTTc                          >  2:984420/1‑100 (MQ=255)
                                                                          cTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCg                         >  2:962974/1‑99 (MQ=255)
                                                                           tAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATCGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCg                         <  2:992186/98‑1 (MQ=255)
                                                                              gTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGt                    <  2:427635/100‑1 (MQ=255)
                                                                              gTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGt                    >  2:1204023/1‑100 (MQ=255)
                                                                              gTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGt                    >  2:490851/1‑100 (MQ=255)
                                                                              attgaaaaaaGCCAGTTCAAATGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGt                    >  2:52668/9‑100 (MQ=38)
                                                                              attgaaaaaaGCCAGTTCAAATGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGt                    >  1:950339/9‑100 (MQ=38)
                                                                               tACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTATTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTg                   <  2:1302646/100‑1 (MQ=255)
                                                                                aCAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGc                  >  1:2160953/1‑100 (MQ=255)
                                                                                aCAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGc                  <  1:1389786/100‑1 (MQ=255)
                                                                                 cAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGcc                 >  1:817271/1‑100 (MQ=255)
                                                                                 cAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGcc                 <  2:391606/100‑1 (MQ=255)
                                                                                  aaCTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCa                <  2:1045988/100‑1 (MQ=255)
                                                                                   aCTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAg               <  2:1732040/100‑1 (MQ=255)
                                                                                     aaaGCCAGTTCAAATGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGtt             >  1:802399/2‑100 (MQ=255)
                                                                                      aaGCCAGTTCAAATGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTGCTCTTTCGGCAGTGCCAGtt             >  1:108369/1‑99 (MQ=255)
                                                                                       aGCCAGTTCAAATGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGttct           >  1:610170/1‑100 (MQ=255)
                                                                                        gCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGttctt          >  1:2124406/1‑100 (MQ=255)
                                                                                         ccAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGttcttc         >  2:10984/1‑100 (MQ=255)
                                                                                         ccAGTTCATTTGAACTGGATTTTTTCAATTCATTGTGAGGATAGTTTACTGACTAGATTTGCATTTCCTGCAACTTCTCTTTCGGCAGTGCCAGttcttc         <  2:1044271/100‑1 (MQ=255)
                                                                                         ccAGTTCATGTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGttcttc         <  1:1181447/100‑1 (MQ=255)
                                                                                              tCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGc    <  2:422709/100‑1 (MQ=255)
                                                                                              tCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGc    <  2:422297/100‑1 (MQ=255)
                                                                                                aTTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTg  <  1:202571/100‑1 (MQ=255)
                                                                                                aTTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTg  >  1:1302875/1‑100 (MQ=255)
                                                                                                aTTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTg  >  2:2128224/1‑100 (MQ=255)
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TTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTG  >  NC_000913/2814077‑2814272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: