Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,838,784	A→G	100%	C161R (TGC→CGC)	ascG ←	asc operon transcriptional repressor; prpBC operon repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,838,784	0	A	G	100.0%	311.8 / NA	89	C161R (TGC→CGC)	ascG	asc operon transcriptional repressor; prpBC operon repressor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (49/40);  total (49/40)

GTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCACCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAATGATGTCATCGATCTCATCCACGCT  >  NC_000913/2838687‑2838880                                                                                                  |                                                                                                 gtggGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGcc                                                                                                <  1:1029472/100‑1 (MQ=255)  tggGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGTGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCa                                                                                               >  1:317287/1‑100 (MQ=255)   gggggAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAg                                                                                              <  2:1050430/100‑1 (MQ=255)   gggggAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAg                                                                                              >  2:1164683/1‑100 (MQ=255)   gggggAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAg                                                                                              <  2:183154/100‑1 (MQ=255)       gAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGc                                                                                          <  1:664387/100‑1 (MQ=255)       gAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGc                                                                                          >  1:3525/1‑100 (MQ=255)        aaTCCATCGAGCCGGTAAGGAAAGCACTCTCCTGATGCCCGGCGCTTATAAACTCTGCCACGGTGTTAACACGGGGCTGTTTATGATCGCGCCAGACGc                                                                                          >  2:1928620/1‑99 (MQ=255)           ccATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATg                                                                                      >  1:1264592/1‑100 (MQ=255)            cATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATgg                                                                                     <  2:2016457/100‑1 (MQ=255)            cATCGAGCAGGTAAGGAAAGCAATCTACTGATGCACGGCGTTTATAAAGTCTGCGACGGGGTTAAAACTGGTCTGTGTATGATCGCGCCAGAAGATATg                                                                                      >  2:2084255/1‑99 (MQ=255)              tCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGgct                                                                                   >  1:202615/1‑100 (MQ=255)                gAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGgctgc                                                                                 <  2:193220/100‑1 (MQ=255)                 aGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGgctgct                                                                                <  1:1268101/100‑1 (MQ=255)                    cGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGtt                                                                             <  1:314750/100‑1 (MQ=255)                    cGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGtt                                                                             >  1:1686688/1‑100 (MQ=255)                    cGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCACGCCAGACGCTATGGCTGCTGtt                                                                             >  1:1052390/1‑100 (MQ=255)                     ggTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGttt                                                                            >  1:2288578/1‑100 (MQ=255)                       tAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACGGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGttttt                                                                          >  2:788742/1‑100 (MQ=255)                        aaGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTg                                                                         <  1:76329/100‑1 (MQ=255)                        aaGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTg                                                                         >  1:1478166/1‑100 (MQ=255)                          ggAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTgcg                                                                       <  1:1307326/100‑1 (MQ=255)                          ggAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTgcg                                                                       >  2:417677/1‑100 (MQ=255)                          ggAAAGCAACCGCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTgcg                                                                       <  2:2028134/100‑1 (MQ=255)                            aaaGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCa                                                                     <  1:1155366/100‑1 (MQ=255)                            aaaGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCa                                                                     <  2:133821/100‑1 (MQ=255)                               gCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAggc                                                                  >  1:1034413/1‑100 (MQ=255)                                  aTCTCCTGATGCAAGGCGTTTATCAATTCTGCCACCGCGTTTTAACCGGTCTATTGATGATCGCGCACGACTCTTTGGCTGCTGTTTTTGCGCAagccgc                                                               >  2:1478276/1‑100 (MQ=255)                                    ctcCTGATGCCCGGCGTTTATCAACTCTGCCACGGCCTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGa                                                             <  1:1672887/100‑1 (MQ=255)                                     tcCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGAt                                                            >  1:2238146/1‑100 (MQ=255)                                       cTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGAtt                                                           >  2:1335/1‑99 (MQ=255)                                       cTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTa                                                          <  2:1892586/100‑1 (MQ=255)                                        tGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTaa                                                         >  2:994798/1‑100 (MQ=255)                                         gATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAg                                                        <  2:516016/100‑1 (MQ=255)                                         gATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAg                                                        >  1:354195/1‑100 (MQ=255)                                          aTGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGc                                                       <  2:2307485/100‑1 (MQ=255)                                            gCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCAc                                                     >  1:2210264/1‑100 (MQ=255)                                              tcttccgatctCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCa                                                   <  2:1755503/90‑1 (MQ=255)                                              ccGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCa                                                   <  2:1342361/100‑1 (MQ=255)                                              ccGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCa                                                   >  1:812106/1‑100 (MQ=255)                                              ccGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCa                                                   >  2:183497/1‑100 (MQ=255)                                               cGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCAt                                                  >  2:701749/1‑100 (MQ=255)                                                 gCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTa                                                <  2:2216065/100‑1 (MQ=255)                                                      tATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGc                                           >  1:1976999/1‑100 (MQ=255)                                                      tATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGc                                           <  1:181229/100‑1 (MQ=255)                                                      tATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGc                                           >  2:2158081/1‑100 (MQ=255)                                                       aTCAACTCTGCCAGGGCGTTAAAACAGGTCTGTTTATGATCGCGTCACACGCTGTGACTGATGTTTTTGCGCAGGCGGCGATTAAGCTCCATTATCGGCt                                          >  1:2376228/1‑100 (MQ=255)                                                       aTCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCt                                          <  2:725151/100‑1 (MQ=255)                                                        tCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTg                                         <  2:1230470/100‑1 (MQ=255)                                                          aaCTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGAc                                       <  1:613370/100‑1 (MQ=255)                                                            ctctGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACtg                                     >  2:365781/1‑100 (MQ=255)                                                               tGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACtgtgn                                  <  2:1321197/100‑2 (MQ=255)                                                                gCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTgcg                                 <  1:1237344/100‑1 (MQ=255)                                                                gCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTgcg                                 >  1:575254/1‑100 (MQ=255)                                                                gCCACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCCGTTTTTTTTTAGTCGTCGATTAAGCACCATTATCGCCTGACTGTgcg                                 >  2:1666927/1‑100 (MQ=255)                                                                 ccACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTgcgc                                >  1:2066148/1‑100 (MQ=255)                                                                  cACGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGcgcg                               <  1:2074836/100‑1 (MQ=255)                                                                   aCGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGt                              >  1:320354/1‑100 (MQ=255)                                                                    cGGCGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGTc                             <  1:1893413/100‑1 (MQ=255)                                                                       cGTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAat                          <  2:1790274/100‑1 (MQ=255)                                                                        gTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAatn                         <  2:1533654/100‑2 (MQ=255)                                                                        gTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAatg                         >  1:280898/1‑100 (MQ=255)                                                                        gTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAatg                         >  1:2217070/1‑100 (MQ=255)                                                                        gTTAAAACTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAatg                         <  1:1354966/100‑1 (MQ=255)                                                                           aaaaCTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAatgatg                      <  1:625620/100‑1 (MQ=255)                                                                           aaaaCTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAatgatg                      >  2:2174773/1‑100 (MQ=255)                                                                           aaaaCTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAatgatg                      >  2:2178743/1‑100 (MQ=255)                                                                            cgatctgTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAATGATGt                     <  1:27365/94‑1 (MQ=255)                                                                            aaaCTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAATGATGt                     >  2:2044563/1‑100 (MQ=255)                                                                             aaCTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAATGATGTc                    >  2:881679/1‑100 (MQ=255)                                                                               cTGGTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAATGATGTCAt                  >  1:2343483/1‑100 (MQ=255)                                                                               cTGGTCTGTGTATGACCGCGCCAGACGCTATGGCTGCTGTGTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGTGTCAatgatg                      <  2:1715679/96‑1 (MQ=255)                                                                                tGGTCTGTTTATGATCGCGCCCGACGATATGGCTGCTGTTTTTGCGCAGGCGGAGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAATGATGTCAt                  <  2:2144895/99‑1 (MQ=255)                                                                                 ggTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCACTGATGTCATCg                >  2:1887008/1‑100 (MQ=255)                                                                                 ggTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAATGATGTCATCg                >  1:1002866/1‑100 (MQ=255)                                                                                  gTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAATGATGtagatcg               >  2:27364/1‑94 (MQ=255)                                                                                  gTCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAATGATGTCATCGa               >  1:1580812/1‑100 (MQ=255)                                                                                   tCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAATGATGTCATCGAt              <  1:2174792/100‑1 (MQ=255)                                                                                   tCTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAATGATGTCATCGAt              <  1:2178762/100‑1 (MQ=255)                                                                                    cTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCCTTATCAGCTGACTGTGCGCGTCACTGATGTCATCTAt              >  2:1668320/1‑99 (MQ=255)                                                                                    cTGTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAATGATGTCATCGAtc             >  1:1558784/1‑100 (MQ=255)                                                                                      gTTTATGATCGCGCCAGACGCTATGGCTGCTGTTTTTTCTTATGTTTCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAATGATGTCATCGAtctc           >  2:968298/1‑100 (MQ=255)                                                                                       tttATGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAATGATGTCATCGATCTCa          >  1:100740/1‑100 (MQ=255)                                                                                          aTGCTCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGTACCATTATCGGCTGACTGTGCGCGTCAATGATGTCATCGATCTCATcc       <  2:143342/100‑1 (MQ=255)                                                                                           tGATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAATGATGTCATCGATCTCATCCa      >  1:1958478/1‑100 (MQ=255)                                                                                            gATCGCGCCAGACGCTATGGCTGCTGTTTTTTTTTTTTTTTCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAATGATGTCATCGATCTCATCCAc     >  2:1756758/1‑100 (MQ=255)                                                                                            gATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGGGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAATGATGTCATCGATCTCAGCCa      <  2:560486/99‑1 (MQ=255)                                                                                            gATCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAATGATGTCATCGATCTCATCCAc     >  1:1332874/1‑100 (MQ=255)                                                                                             aTCGCGCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAATGATGTCATCGATCTCATCCACg    <  1:2114794/100‑1 (MQ=255)                                                                                               cgcgCCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAATGATGTCATCGATCTCATCCACGCt  <  2:2190581/100‑1 (MQ=255)                                                                                                  |                                                                                                 GTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATCAACTCTGCCACGGCGTTAAAACTGGTCTGTTTATGATCGCACCAGACGCTATGGCTGCTGTTTTTGCGCAGGCGGCGATTAAGCACCATTATCGGCTGACTGTGCGCGTCAATGATGTCATCGATCTCATCCACGCT  >  NC_000913/2838687‑2838880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑