Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,194,516	A→G	100%	K551K (AAA→AAG)	yqiK →	PHB family membrane protein, function unknown

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,194,516	0	A	G	100.0%	322.8 / ‑6.7	95	K551K (AAA→AAG)	yqiK	PHB family membrane protein, function unknown
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (51/43);  total (51/44)

CGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGT  >  NC_000913/3194419‑3194609                                                                                                  |                                                                                              cGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGc                                                                                             <  2:2141563/100‑1 (MQ=255)  gCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCa                                                                                            >  2:1071898/1‑100 (MQ=255)  gCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCa                                                                                            >  1:1878907/1‑100 (MQ=255)   cTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCag                                                                                           <  2:1478123/100‑1 (MQ=255)   cTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCag                                                                                           <  2:1655571/100‑1 (MQ=255)   cTGAGTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCag                                                                                           <  2:1625462/100‑1 (MQ=255)    tGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCaga                                                                                          >  1:1741587/1‑100 (MQ=255)     gATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCagag                                                                                         >  2:628227/1‑100 (MQ=255)      aTTGTCTCCTTGCTCAATGAAATTGTCGGTGCAGGCGTCTCACTGGCGGCATTAACTTCACGCTTAACCTCAACAACTCCCGTCGCAGAAACAGCagaat                                                                                        <  2:1650367/100‑3 (MQ=255)      aTTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGt                                                                                        >  1:121422/1‑100 (MQ=255)      aTTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGt                                                                                        <  2:1665899/100‑1 (MQ=255)        tGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTaa                                                                                      >  1:77024/1‑100 (MQ=255)         gACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTaaa                                                                                     >  2:1022877/1‑100 (MQ=255)           cTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAAcc                                                                                   <  1:528406/100‑1 (MQ=255)            tCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAAccc                                                                                  <  1:1890527/100‑1 (MQ=255)             ccTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCct                                                                                 >  1:471842/1‑100 (MQ=255)             ccTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCct                                                                                 >  2:253701/1‑100 (MQ=255)             ccTTGCTCAATGAAATTGGCGATTCAGTAGGCTCACTGGCGGCATTGACTTAATCCTTAACGACAACAACTCCCGGCGAAGAAAAGGCGGCGTaacacct                                                                                 >  2:2059642/1‑95 (MQ=255)              cTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCctc                                                                                >  2:1292283/1‑100 (MQ=255)                  cTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGTCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTcc                                                                            >  2:1098462/1‑100 (MQ=255)                  cTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTcc                                                                            >  1:946532/1‑100 (MQ=255)                    cAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAGGAAAAGGCAGAGTAAACCCTCTTCCAg                                                                          >  2:323063/1‑100 (MQ=255)                    cAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAg                                                                          <  1:712378/100‑1 (MQ=255)                    cAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAg                                                                          >  1:106775/1‑100 (MQ=255)                      aTGAAGTTGGCGTTTCAGGCGGCTCACTGGCTGCATTGACTGGCCCCTTCGCCTCACCAGCTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAgt                                                                         <  2:2018713/99‑2 (MQ=255)                      aTGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGa                                                                        >  2:1845445/1‑100 (MQ=255)                      aTGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGa                                                                        >  2:984143/1‑100 (MQ=255)                      aTGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGa                                                                        >  2:725188/1‑100 (MQ=255)                      aTGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGa                                                                        >  1:2146304/1‑100 (MQ=255)                      aTGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGa                                                                        >  2:1340580/1‑100 (MQ=255)                       tGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGaa                                                                       >  1:87561/1‑100 (MQ=255)                       tGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGaa                                                                       <  2:32072/100‑1 (MQ=255)                        gAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGaat                                                                      >  1:1662027/1‑100 (MQ=255)                           aTTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGaataat                                                                   >  1:860160/1‑100 (MQ=255)                            ttGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATc                                                                  <  1:706508/100‑1 (MQ=255)                            ttGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATc                                                                  >  2:2352908/1‑100 (MQ=255)                             tGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATcc                                                                 <  1:614740/100‑1 (MQ=255)                              ggCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATccc                                                                >  1:830499/1‑100 (MQ=255)                               gCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCt                                                               <  1:956801/100‑1 (MQ=255)                                cGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTg                                                              >  2:1890827/1‑100 (MQ=255)                                 gTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAATAAACACTCTACCAGGAATAATCCCTgg                                                             >  2:189890/1‑100 (MQ=255)                                  tttCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGc                                                            <  2:1160144/100‑1 (MQ=255)                                   ttCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGcc                                                           <  2:1312171/100‑1 (MQ=255)                                   ttCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGcc                                                           >  1:1312178/1‑100 (MQ=255)                                    tCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGccc                                                          <  1:882532/100‑1 (MQ=255)                                      aGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCaa                                                        >  2:654693/1‑100 (MQ=255)                                       ggcggcTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCaaa                                                       <  1:1267361/100‑1 (MQ=255)                                          ggcTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAg                                                    >  1:1129195/1‑100 (MQ=255)                                           gcTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGc                                                   >  1:1117126/1‑100 (MQ=255)                                             taaCTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCg                                                 >  1:1614984/3‑100 (MQ=255)                                              cACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCgg                                                <  1:1626914/100‑1 (MQ=255)                                              cACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCgg                                                <  2:155572/100‑1 (MQ=255)                                               aCTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCATGAATAATCCCTGGCCCAAAAAGCCggg                                               <  2:362334/100‑1 (MQ=255)                                               aCTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCggg                                               <  1:2076159/100‑1 (MQ=255)                                               aCTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCggg                                               >  1:1329201/1‑100 (MQ=255)                                                cTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCgggg                                              >  1:1582825/1‑100 (MQ=255)                                                  ggcggcATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAt                                            >  1:2298899/1‑100 (MQ=255)                                                     ggcATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAtttt                                         <  2:764938/100‑1 (MQ=255)                                                      gcATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAttttt                                        >  1:278028/1‑100 (MQ=255)                                                      gcATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAttttt                                        <  1:2076943/100‑1 (MQ=255)                                                       cATTGACTTCACCCTTAACCTCCACAACTCCCCTCGAAGAAAAGGCAGAGTAAACCCTCCTCCAGGACTAATCCCCGGCCCAAAAAGCCGGGGActcttt                                       >  2:1078665/1‑100 (MQ=255)                                                       cATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAtttttt                                       <  1:612747/100‑1 (MQ=255)                                                       cATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAtttttt                                       <  1:876213/100‑1 (MQ=255)                                                       cATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCCCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAtttttt                                       >  2:477844/1‑100 (MQ=255)                                                        aTTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCGGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTta                                      <  2:2251337/100‑1 (MQ=255)                                                        aTTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTta                                      >  2:391795/1‑100 (MQ=255)                                                        aTTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTta                                      <  1:320790/100‑1 (MQ=255)                                                        aTTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTta                                      <  1:253703/100‑1 (MQ=255)                                                         ttGACTTCACCCTTAACCTCAACAGCCCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTATCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTta                                      <  2:787351/99‑1 (MQ=38)                                                         ttGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTtat                                     <  2:645092/100‑1 (MQ=255)                                                         ttGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTtat                                     <  2:1973239/100‑1 (MQ=255)                                                         ttGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTtat                                     <  1:531812/100‑1 (MQ=255)                                                           gACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTatat                                   >  1:540016/1‑100 (MQ=255)                                                            aCTTCACCCTAAACCTCAACACCTCCCGTCGAATAAAAGTCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATc                                  <  2:546000/100‑1 (MQ=37)                                                             cTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCt                                 >  1:939668/1‑100 (MQ=255)                                                              ttCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTg                                <  1:674557/100‑1 (MQ=255)                                                              ttCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTg                                <  2:330815/100‑1 (MQ=255)                                                               ccACCCTTAACCTCAACAACTCCCGCCGCAGCAACGACAGAGTAAACCCTCTTCTACCAACCATCCCTGCCACAACAATCTCGGGAtttcatccatc                                  >  2:1444298/2‑89 (MQ=255)                                                                cACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCg                              >  1:503002/1‑100 (MQ=255)                                                                  cccTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGtt                            >  1:1912020/1‑100 (MQ=255)                                                                   ccTTAACCTCAACAACTCCCGTCGAAGAAAAGGCATAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTc                           <  1:1655090/100‑1 (MQ=255)                                                                   ccTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTc                           >  2:2189402/1‑100 (MQ=255)                                                                     ttAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCg                         <  1:2103950/100‑1 (MQ=255)                                                                       aaCCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCt                       >  1:22697/1‑100 (MQ=255)                                                                         ccTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTaa                     >  1:2044586/1‑100 (MQ=255)                                                                            caacaaCTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTaaaaa                  <  1:2085105/100‑1 (MQ=255)                                                                            caacaaCTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTaaaaa                  >  1:775738/1‑100 (MQ=255)                                                                            caacaaCTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTaaaaa                  >  1:52000/1‑100 (MQ=255)                                                                                aacTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGc              <  1:1845459/100‑1 (MQ=255)                                                                                   tCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCaaa           <  1:202074/100‑1 (MQ=255)                                                                                    cccGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCACAt          >  2:149494/1‑100 (MQ=255)                                                                                     ccGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATg         <  1:1071902/100‑1 (MQ=255)                                                                                         cGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCa     >  1:159843/1‑100 (MQ=255)                                                                                           aagaaAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAgg   <  1:2066319/100‑1 (MQ=255)                                                                                            agaaAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgt  <  1:1235284/100‑1 (MQ=255)                                                                                                  |                                                                                              CGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGT  >  NC_000913/3194419‑3194609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑