Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	410,479	A→G	100%	S112S (TCA→TCG)	mak →	manno(fructo)kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	410,479	0	A	G	98.8%	336.4 / ‑5.0	100	S112S (TCA→TCG)	mak	manno(fructo)kinase
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base G (51/46);  total (51/49)
Fisher's exact test for biased strand distribution p-value = 2.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAACCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGG  >  NC_000913/410382‑410573                                                                                                  |                                                                                               cAACCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGa                                                                                              >  2:1588973/1‑100 (MQ=255)  aaCCTGGCTCAACGGTCAGCCATTCTATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCTGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCCGa                                                                                              <  2:1938965/99‑1 (MQ=255)  aaCCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGaa                                                                                             >  2:1545918/1‑100 (MQ=255)   aCCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAg                                                                                            <  1:1815707/100‑1 (MQ=255)   aCCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAg                                                                                            <  1:1467657/100‑1 (MQ=255)   aCCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAAGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAg                                                                                            <  1:755258/100‑1 (MQ=255)     cTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCa                                                                                          <  2:1508306/100‑1 (MQ=255)      tGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCa                                                                                          <  1:884879/99‑1 (MQ=255)       ggCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCGGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGt                                                                                        >  1:724124/1‑100 (MQ=255)       ggCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGt                                                                                        >  2:772003/1‑100 (MQ=255)       ggCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGt                                                                                        >  1:2033794/1‑100 (MQ=255)        gCTGAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTa                                                                                       <  2:1446562/100‑1 (MQ=255)         cTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAg                                                                                      <  1:314293/100‑1 (MQ=255)         cTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAg                                                                                      >  1:191378/1‑100 (MQ=255)         cTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAg                                                                                      <  1:1912642/100‑1 (MQ=255)           cagtggTCAGCCATTTGATAAAGAGTTAGGCGCGAGGGTGCAGCGGGAAGTTCGGCTGGCAAATGACGCTAACTGTCTGGGTGTTTCGTAAGCAGTAg                                                                                      >  2:291868/5‑98 (MQ=255)           cAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGa                                                                                     >  2:965462/1‑99 (MQ=255)           cAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGAt                                                                                    >  2:1629008/1‑100 (MQ=255)           cAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGAt                                                                                    <  1:65864/100‑1 (MQ=255)           cAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGGTTCGGGAGCAGTAGa                                                                                     >  2:2367863/1‑99 (MQ=255)            aaCGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATg                                                                                   <  2:257738/100‑1 (MQ=255)              cGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGgc                                                                                 >  1:305096/1‑100 (MQ=255)              cGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGgc                                                                                 >  1:149203/1‑100 (MQ=255)                gTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGgcgc                                                                               >  1:2124460/1‑100 (MQ=255)                   aGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGc                                                                            <  1:646522/100‑1 (MQ=255)                   aGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCGGGCGGTGTCGGAAGCAGTAGATGGcgcg                                                                              >  2:204442/1‑98 (MQ=255)                      cATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCCGGCGGTCTCGGAAGGAGTAGATgggtagcgaac                                                                         >  2:1825046/1‑91 (MQ=255)                       aTTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCg                                                                        <  1:580200/100‑1 (MQ=255)                         tCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCggg                                                                      <  1:1834070/100‑1 (MQ=255)                         tCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCggg                                                                      <  2:2124443/100‑1 (MQ=255)                           ggtAAAGGCTTAAGCGCGAGGTTGCAGCGGGAAGGGTGGCTGGCAAATGACGCTAACGGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAg                                                                    <  2:2105598/98‑1 (MQ=255)                           gATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAg                                                                    <  2:1219180/100‑1 (MQ=255)                           gATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAg                                                                    <  1:1633921/100‑1 (MQ=255)                           gATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAg                                                                    >  2:1716872/1‑100 (MQ=255)                            aTAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAgc                                                                   >  1:522641/1‑100 (MQ=255)                             tAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAgcg                                                                  <  1:24001/100‑1 (MQ=255)                             tAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAgcg                                                                  >  2:1878381/1‑100 (MQ=255)                              aaaGACTTAAGCGCGAGGTTGCGGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGGgcg                                                                  >  2:106917/1‑99 (MQ=255)                              aaaGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAgcgc                                                                 >  1:1471671/1‑100 (MQ=255)                                aGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCa                                                                <  2:1352257/99‑1 (MQ=255)                                 gACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGa                                                              >  1:256760/1‑100 (MQ=255)                                   cTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACg                                                            >  1:1787195/1‑100 (MQ=255)                                    ttAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACgg                                                           <  1:102245/100‑1 (MQ=255)                                    ttAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACgg                                                           >  1:1169338/1‑100 (MQ=255)                                        gcgcGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTAtt                                                       >  1:1003881/1‑100 (MQ=255)                                            gAGGGTGGAGCGGGAAGTGCGGCTGGCATATGACGCTTACGGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGct                                                   <  2:454712/100‑2 (MQ=255)                                              ggTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGt                                                 >  1:907156/1‑100 (MQ=255)                                              ggTGGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGt                                                 <  1:640606/100‑1 (MQ=255)                                               gTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTg                                                >  1:1643426/1‑100 (MQ=255)                                               gTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTg                                                >  1:2219066/1‑100 (MQ=255)                                               gTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTCGCCGTg                                                >  1:764439/1‑100 (MQ=255)                                                ttGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGa                                               <  1:670541/100‑1 (MQ=255)                                                   cAGCGGGAAGAGCAGCTGGCAAATGACGCTAACTGTCTGGAGGTTTCGGAAGCAGAAGAGGGCACAGCGGAGGGAGCTCAGACGGGATTTGCCGttatta                                            >  2:455379/1‑100 (MQ=255)                                                      cGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCg                                         <  1:1960269/100‑1 (MQ=255)                                                      cGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCg                                         >  1:1485699/1‑100 (MQ=255)                                                       gggAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCgg                                        <  1:1022607/100‑1 (MQ=255)                                                       gggAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCgg                                        <  1:442525/100‑1 (MQ=255)                                                       gggAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCgg                                        >  2:317937/1‑100 (MQ=255)                                                        ggAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGc                                       <  1:2114838/100‑1 (MQ=255)                                                           aGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACg                                    <  1:372037/100‑1 (MQ=255)                                                           aGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACg                                    <  1:804987/100‑1 (MQ=255)                                                            gTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACgg                                   >  1:1183682/1‑100 (MQ=255)                                                            gTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACgg                                   <  1:1410730/100‑1 (MQ=255)                                                            gTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACgg                                   >  1:105654/1‑100 (MQ=255)                                                              gCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACggg                                  <  2:414229/99‑1 (MQ=255)                                                               cGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGAt                                <  2:1326468/100‑1 (MQ=255)                                                                 gCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATgc                              <  2:180666/100‑1 (MQ=255)                                                                        aaTGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCgg                       >  2:1789830/1‑100 (MQ=255)                                                                        aaTGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCgg                       >  2:1705022/1‑100 (MQ=255)                                                                        aaTGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCgg                       >  1:837990/1‑100 (MQ=255)                                                                         aTGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCggg                      <  1:1782924/100‑1 (MQ=255)                                                                           gACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCg                    >  2:1972649/1‑100 (MQ=255)                                                                            aCGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGt                   >  2:56750/1‑100 (MQ=255)                                                                             cGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTg                  >  1:223936/1‑100 (MQ=255)                                                                             cGCTAACCGTCTGGCGGTTTCGGAAGCAGTGGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTg                  <  2:1366762/100‑1 (MQ=255)                                                                              gCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTgg                 <  1:2044819/100‑1 (MQ=255)                                                                              gCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTgg                 <  2:2096033/100‑1 (MQ=255)                                                                              gCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTgg                 <  2:2219046/100‑1 (MQ=255)                                                                               cTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGc                >  2:357614/1‑100 (MQ=255)                                                                                  aCTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCAtt             <  1:581138/100‑1 (MQ=255)                                                                                   cTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCCTTc            >  1:2277924/1‑100 (MQ=255)                                                                                   cTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTc            >  2:2154058/1‑100 (MQ=255)                                                                                   cTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTc            >  2:2147419/1‑100 (MQ=255)                                                                                     gTCTGGCGGGTGAAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGGCGGTATTTGGCGTGATTAGCGGCACGGGATGCAGCGCGGGCGTGGCATTc            <  2:1436690/98‑1 (MQ=255)                                                                                      tCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAAt         >  2:1446905/1‑100 (MQ=255)                                                                                      tCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAAt         <  1:1788354/100‑1 (MQ=255)                                                                                      tCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAAt         <  1:1746676/100‑1 (MQ=255)                                                                                       cTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATg        <  2:191377/100‑1 (MQ=255)                                                                                        tGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATgg       >  2:621378/1‑100 (MQ=255)                                                                                         ggCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATggg      <  1:1272778/100‑1 (MQ=255)                                                                                         ggCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATggg      >  1:1689360/1‑100 (MQ=255)                                                                                         ggCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATggg      <  1:740943/100‑1 (MQ=255)                                                                                         ggCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATggg      <  2:964906/100‑1 (MQ=255)                                                                                         ggCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCAGGCGTGGCATTCAATggg      >  2:1326471/1‑100 (MQ=255)                                                                                          gCGGTTTCGGAAGCAGGAGATGGGGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTGAATgggc     >  2:181283/1‑100 (MQ=255)                                                                                           cGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGAGGCGCGGGCGTGGCATTCAATgg       >  2:773698/1‑97 (MQ=255)                                                                                            ggTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATgggcgg   <  1:458340/100‑1 (MQ=255)                                                                                             gTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATgggcggg  <  2:17068/100‑1 (MQ=255)                                                                                             gTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATgggcggg  >  2:1718265/1‑100 (MQ=255)                                                                                             gTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCACAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATgggcggg  >  1:1741833/1‑100 (MQ=255)                                                                                                  |                                                                                               CAACCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGG  >  NC_000913/410382‑410573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑