| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 460,106 | A→T | 100% | I407F (ATC→TTC) | lon → | DNA‑binding ATP‑dependent protease La |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 460,106 | 0 | A | T | 98.8% | 270.9 / ‑4.4 | 80 | I407F (ATC→TTC) | lon | DNA‑binding ATP‑dependent protease La |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base T (41/38); total (42/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
GCGTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTC > NC_000913/460009‑460200 | gCGTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTc < 2:932877/100‑1 (MQ=255)gCGTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTc < 2:1195563/100‑1 (MQ=255)gCGTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTc < 1:1185135/100‑1 (MQ=255) cGTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTcc > 1:1641869/1‑100 (MQ=255) tAACTATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTcc > 2:2158902/1‑98 (MQ=255) tAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCa < 1:1852773/99‑1 (MQ=255) tAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAg < 1:1315887/100‑1 (MQ=255) aaTATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGaa < 1:788849/100‑1 (MQ=255) aaTATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGaa < 1:787039/100‑1 (MQ=255) aaTATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGaa > 1:2358516/1‑100 (MQ=255) atatGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGaaa < 1:868054/100‑1 (MQ=255) atGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAAt < 1:1967489/100‑1 (MQ=255) gTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATgg < 1:1596446/100‑1 (MQ=255) gTCCGTATGGCGCGGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATgg > 1:1622570/1‑100 (MQ=255) tCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGc < 2:449374/100‑1 (MQ=255) tCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGc < 1:2169979/100‑1 (MQ=255) ccGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCg > 1:797112/1‑100 (MQ=255) gTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGaa > 1:1289514/1‑100 (MQ=255) gTATGACGCTGGGTGGCGTGCGTGATGAAGCGGAACTCCGTGGTCACCGCAGTACTTAAATCGGTTCTATGCAGAGTAAACTGTTCCAGAAAATGGCGaa > 2:2106737/1‑100 (MQ=255) tATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGaaa > 1:1936676/1‑100 (MQ=255) aTGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAg > 1:1785895/1‑100 (MQ=255) ggCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTg > 1:1777472/1‑100 (MQ=255) ggCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTg > 2:1262035/1‑100 (MQ=255) ggCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTg > 2:1701834/1‑100 (MQ=255) cgcTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTggg > 2:1770709/1‑100 (MQ=255) cgcTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTggg < 2:1193834/100‑1 (MQ=255) cgcTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTggg > 2:1139932/1‑100 (MQ=255) cgcTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTggg < 1:1043119/100‑1 (MQ=255) cgcTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTggg > 2:541711/1‑100 (MQ=255) cTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCg < 1:1985203/100‑1 (MQ=255) ggcggcGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGa < 2:2228723/100‑1 (MQ=255) ggcggcGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGa > 1:2030512/1‑100 (MQ=255) gcggcgTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGaa < 2:1728771/100‑1 (MQ=255) gcggcgTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGaa < 1:1072096/100‑1 (MQ=255) gcggcgTGCGTGAAGAAGCGGAAATCCGTGGTCACCGCAGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGaa > 2:1469049/1‑100 (MQ=255) cggagTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCCTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGaaa < 2:887790/100‑1 (MQ=255) cgtgcgtgATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAAc < 2:1391161/100‑1 (MQ=255) tgaAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTc > 2:660452/1‑100 (MQ=255) gaAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTcc > 2:1504853/1‑100 (MQ=255) gaAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTcc > 1:293143/1‑100 (MQ=255) gaAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTcc > 2:1135352/1‑100 (MQ=255) aaGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCt < 2:1233968/100‑1 (MQ=255) aGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTg < 1:541713/100‑1 (MQ=255) aGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTg > 2:795864/1‑100 (MQ=255) gCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGc > 1:1376080/1‑100 (MQ=255) gCGGAAATCCCTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGc < 1:2302801/100‑1 (MQ=255) cGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCt > 1:264976/1‑100 (MQ=255) ggAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTc < 2:544372/100‑1 (MQ=255) aaTCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGAt < 1:44326/100‑1 (MQ=255) tCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAGCTGTTTCAGAAAATGGCGAAAGTGGGCGTGAAAAACACGCTGTACCTGCTCGATg > 2:66263/1‑99 (MQ=255) tCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATga > 1:743071/1‑100 (MQ=255) ngTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATgaga > 2:1765649/2‑100 (MQ=255) gTGGTCACCGCCGTACTTACATCGTTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATgagan < 2:854686/100‑2 (MQ=255) gTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGAt < 1:1570659/100‑1 (MQ=255) tGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATc < 1:1093787/100‑1 (MQ=255) ggTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCCGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCg > 2:222763/1‑100 (MQ=255) gTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGa > 2:637777/1‑100 (MQ=255) tCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGAc > 1:1309360/1‑100 (MQ=255) tCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGAc > 2:670402/1‑100 (MQ=255) ccgccgTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACGCGCTGTTCCTGCTCGATGAGATCGACaaa > 2:2071114/1‑100 (MQ=255) cgccgTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACaaaa > 2:2235804/1‑100 (MQ=255) gccgTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAAt > 1:1650560/1‑100 (MQ=255) gccgTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAAt > 2:368753/1‑100 (MQ=255) gccgTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAAt < 2:1815904/100‑1 (MQ=255) gccgTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAAt < 2:2052839/100‑1 (MQ=255) gTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATg > 2:1756874/1‑98 (MQ=255) cTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGtcttc > 1:2194830/1‑100 (MQ=255) aCATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGTGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGa < 1:1770723/100‑1 (MQ=255) aCATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGa < 1:1139937/100‑1 (MQ=255) cATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGAc > 2:697509/1‑100 (MQ=255) cATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCGCGATGAGATCGACAAAATGTCTTCTGAc > 2:309193/1‑100 (MQ=255) gTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCg < 1:795866/100‑1 (MQ=255) tATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGc > 1:2029872/1‑100 (MQ=255) tATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGc < 1:1441002/100‑1 (MQ=255) aTGCCGGCCAAAATGTACCACAAAATGGCCAAAGTGGGCGTCAAAAACCCGCTGTTCATGCTCGATCAGCTCGACAAAATGTCTTCTGACATGCGTGGCg < 2:823319/100‑1 (MQ=255) ccGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATc > 2:818040/1‑100 (MQ=255) tAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAACCCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCg < 2:2358017/97‑1 (MQ=255) aaaCTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCct < 1:228342/100‑1 (MQ=255) aaCTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCctc > 1:2200693/1‑100 (MQ=255) aaCTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCctc < 1:1858299/100‑1 (MQ=255) | GCGTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTC > NC_000913/460009‑460200 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |