Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,838,723 | T→C | 16.7% | L159S (TTG→TCG) | yicL → | EamA family inner membrane putative transporter |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,838,723 | 0 | T | C | 16.7% | 185.8 / 29.4 | 83 | L159S (TTG→TCG) | yicL | EamA family inner membrane putative transporter |
Reads supporting (aligned to +/- strand): ref base T (34/35); new base C (6/8); total (40/43) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.73e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01 |
CATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAG > NC_000913/3838625‑3838822 | cATTCTGGTTTTCTTCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCgttg < 2:443143/100‑1 (MQ=255) cATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCgttg > 2:2123339/1‑100 (MQ=255) ttCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCgttgtt < 1:152780/100‑1 (MQ=255) tCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTc < 2:628108/100‑1 (MQ=255) cTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTCGTTCt > 1:414046/1‑100 (MQ=255) cTGGTTTTCTGCGCGATTTTGACATCGCTGGTCGGGACTTTTTCATTGGTGACACACGGTAATCTGACGTCATTATCGATCTCTCCTGCCGCGTTGATCt > 2:58583/1‑100 (MQ=255) atGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCt > 2:1132744/2‑100 (MQ=255) tGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTg > 1:2131634/1‑100 (MQ=255) gTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTggg < 2:1627008/100‑1 (MQ=255) ttttCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGGCATTATCGATCTCTCCTGCCGCGTTGTTCTgggg > 1:575450/1‑100 (MQ=255) tttCTGCGCTATTTTGACATCGCTGGGCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTgggg < 1:1137311/99‑1 (MQ=255) cTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCAtt > 1:248642/1‑100 (MQ=255) tGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTg > 1:981293/1‑100 (MQ=255) gcgcTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGc > 2:767301/1‑100 (MQ=255) gcgcTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGCGGCAATg > 2:2115553/1‑99 (MQ=255) cgcTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGcc < 1:1518764/100‑1 (MQ=255) gcTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTCGTTCTGGGGCATTGCCt < 2:957993/100‑1 (MQ=255) gcTATTTTGACATCGCTGGTCGGGAC‑TTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTCGTTCTGGGGCATTGCCTc < 1:2322297/100‑1 (MQ=255) cTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTc > 2:2284750/1‑100 (MQ=255) tATTTTGACATCGCTTGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTCGTTCTGGGGCATTGCCTCg < 2:1087437/100‑1 (MQ=255) tATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCg < 1:2367273/100‑1 (MQ=255) tATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCg > 2:1367205/1‑100 (MQ=255) aTTTTGACATCGCTGTTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCgg < 1:2347044/100‑1 (MQ=255) aTTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCgg > 1:532736/1‑100 (MQ=255) tttGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCa < 1:1882442/100‑1 (MQ=255) gACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCAttt < 1:786583/100‑1 (MQ=255) aCATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTtg < 1:1696927/100‑1 (MQ=255) cATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTtgc < 1:2074956/100‑1 (MQ=255) cATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTtgc > 2:268215/1‑100 (MQ=255) tCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTtgct < 1:1804099/99‑1 (MQ=255) cGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTtgctgc < 2:2001910/100‑1 (MQ=255) cGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTtgctgc < 2:2005549/100‑1 (MQ=255) cGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTCGTTCTGGGGCATTGCCTCGGCATTtgctgc < 2:974079/100‑1 (MQ=255) tGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTtgctgctgc > 1:2305074/1‑100 (MQ=255) ggACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCGCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCta < 2:2235782/100‑1 (MQ=255) gACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTCCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCtat < 2:1275367/100‑1 (MQ=255) gACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTCGGTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCtat < 2:1470022/100‑1 (MQ=255) cTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATac > 2:1047884/1‑100 (MQ=255) cTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATac > 1:1854418/1‑100 (MQ=255) cTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATac > 1:2181155/1‑100 (MQ=255) ttttttATTGTTGACACACGGTAATCCGACGTCATGATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATacc < 1:2130603/100‑1 (MQ=255) ttttttATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATacc < 1:1382967/100‑1 (MQ=255) tttttATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTCGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATacca > 2:616619/1‑100 (MQ=255) tttATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATaccacc < 2:1289628/100‑1 (MQ=255) ttattaGTCACACACGGTAATCTGCCGTACTTATCGATCTCTCCTGCCTCGTCGTTCTGGGGCATTGCCTCGGCTTTTGCTGCTGCATACTGTactccc < 2:730118/99‑5 (MQ=255) ttATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCt < 2:95577/100‑1 (MQ=255) aTTGGTGACACACAGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTCGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTAt > 2:1472251/1‑100 (MQ=255) ttGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTCGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATc < 1:268854/100‑1 (MQ=255) tGGTGACACACGGTAATCCGCCGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATcc > 1:326562/1‑100 (MQ=255) ggTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATccc < 1:942985/100‑1 (MQ=255) ggTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATccc < 1:2284771/100‑1 (MQ=255) tGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTc < 1:393109/100‑1 (MQ=255) tGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTc > 2:1180474/1‑100 (MQ=255) tGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTc < 1:563838/100‑1 (MQ=255) gccactcGGTAATCCTACGTCATTATCGTTCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCa < 2:632962/98‑1 (MQ=255) gACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCa > 2:188922/1‑100 (MQ=255) cacacGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATCCCACCTATCCCTCAAc > 1:2233002/1‑100 (MQ=255) cacacGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAAc > 2:347205/1‑100 (MQ=255) cacacGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAAc < 1:1976395/100‑1 (MQ=255) cacacGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAAc > 2:1613030/1‑100 (MQ=255) cacacGGTAATCCGACGTCATTATCGATCTATCATGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCAATCCCTCAAc > 2:1572085/1‑100 (MQ=255) aaTCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTg > 2:1556392/1‑100 (MQ=255) aaTCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTg > 1:962527/1‑100 (MQ=255) aTCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGc < 1:2367201/100‑1 (MQ=255) tCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGcc > 2:2138246/1‑100 (MQ=255) ccGACGTCATTATCGATCTCTCCTGCCGCGTCGTTCTGGGGCATTGCCTCGGCCTTTGCTGCTGCATTCTATACCACCTATCCCTCAACACTAATTGccc > 2:687099/1‑100 (MQ=255) gACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGAGCATTGCCTCGGCATTTGCTGATGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGc > 1:1388554/1‑100 (MQ=255) cGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTa < 1:2137478/100‑1 (MQ=255) tCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATg < 1:782539/100‑1 (MQ=255) cATTATCGATCTCTCCTGCCGCGTCGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATgg > 1:2318428/1‑100 (MQ=255) aTTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGc < 2:2049114/100‑1 (MQ=255) ttATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCa < 1:2008845/100‑1 (MQ=255) tATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCAc < 1:80687/100‑1 (MQ=255) aTCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACg > 1:993932/1‑100 (MQ=255) aTCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACg < 2:1551282/100‑1 (MQ=255) aTCGATCTCTCCTGCCGCGTCGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACg < 2:1786677/100‑1 (MQ=255) tCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGt > 1:1229908/1‑100 (MQ=255) cGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGtt > 2:92946/1‑100 (MQ=255) gATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTa > 1:2279355/1‑100 (MQ=255) gATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTa > 1:1526918/1‑100 (MQ=255) tctctcCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTAcc < 1:891202/100‑1 (MQ=255) tctcCTGCCGCGTCGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAg > 1:1069786/1‑100 (MQ=255) ctcCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAgt > 1:409788/1‑100 (MQ=255) ctcCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAg < 2:701394/99‑1 (MQ=255) gcgTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCt > 2:303336/1‑100 (MQ=255) cgTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTg < 1:2138263/100‑1 (MQ=255) gttgttCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTgg > 1:1368950/1‑100 (MQ=255) tgttCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAg < 1:767303/100‑1 (MQ=255) | CATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAG > NC_000913/3838625‑3838822 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |