Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,481,904 (A)8→7 coding (273/1539 nt) emrY ← putative multidrug efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,481,8970A.95.5% 470.0 / 13.5 112coding (280/1539 nt)emrYputative multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base A (1/4);  new base . (56/51);  total (57/55)
Fisher's exact test for biased strand distribution p-value = 2.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

CAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAA  >  NC_000913/2481799‑2481992
                                                                                                  |                                                                                               
cAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGaa                                                                                                >  2:1919182/1‑100 (MQ=255)
 aGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGaaa                                                                                               <  1:2671872/100‑1 (MQ=255)
  ggggAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGaaaa                                                                                              <  1:530521/98‑1 (MQ=255)
  gTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGaaaa                                                                                              >  1:1436533/1‑100 (MQ=255)
   tGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGaaaaa                                                                                             >  2:2723080/1‑100 (MQ=255)
      aaTTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGaaaaaaag                                                                                          <  2:1108180/100‑2 (MQ=255)
      aaTTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGAAAAAGaaaaaaag                                                                                          <  2:3277898/100‑2 (MQ=255)
      aaTTAACGCCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGaaaaaaa                                                                                           <  2:3046858/99‑1 (MQ=255)
         tAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGaaaaaaagtga                                                                                       >  1:2279353/1‑96 (MQ=255)
         tAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGaaaaaaagtg                                                                                        >  1:893205/1‑96 (MQ=255)
          aaCGGCCCCGGCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTAGACATTAATGAAGACAGCGAAAAAAAGTGAc                                                                                     <  2:2205509/100‑1 (MQ=255)
            cGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACt                                                                                    >  1:2477511/1‑99 (MQ=255)
              gCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGaa                                                                                 <  1:194806/100‑1 (MQ=255)
                cccGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAg                                                                               >  1:2052372/1‑100 (MQ=255)
                 ccGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGt                                                                              <  2:2564621/100‑1 (MQ=255)
                      aTTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTaaaaa                                                                         <  1:2858313/100‑1 (MQ=255)
                       ttAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAaat                                                                        >  1:2635042/1‑100 (MQ=255)
                       ttAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAaat                                                                        >  2:3231181/1‑100 (MQ=255)
                          aCCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAaataat                                                                     <  1:2634326/100‑1 (MQ=255)
                          aCCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAaataat                                                                     >  1:2400411/1‑100 (MQ=255)
                           ccccTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATc                                                                    >  1:1628226/1‑100 (MQ=255)
                           ccccTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATc                                                                    <  1:1461771/100‑1 (MQ=255)
                            cccTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCt                                                                   >  1:695465/1‑100 (MQ=255)
                            cccTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCt                                                                   >  1:2062995/1‑100 (MQ=255)
                              cTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTa                                                                 <  1:838188/100‑1 (MQ=255)
                               tGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTaa                                                                >  2:2310097/1‑100 (MQ=255)
                               tGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTaa                                                                <  2:2973819/100‑1 (MQ=255)
                               tGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACAGAAAGTAAAAATAATCTTaa                                                                >  2:1487033/1‑100 (MQ=255)
                                 aaCGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAAtt                                                              >  1:1396426/1‑100 (MQ=255)
                                    gACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGc                                                           <  1:117213/100‑1 (MQ=255)
                                     aCTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGcc                                                          <  1:1711024/100‑1 (MQ=255)
                                     aCTCTAAAAAATATCAGCACAACAAGATTAGTCGATAGGCTATACATTAATAAAAAAAAAAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGc                                                           <  2:2898200/99‑1 (MQ=255)
                                     aCTCTAAAAAATAGCAGCACATCAAGCTTGGTCGATAGGCTACACATTAATGAAGACGGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCg                                                            >  2:829507/1‑98 (MQ=255)
                                      ctctAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGcc                                                          <  1:1025831/100‑1 (MQ=255)
                                      ctctAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCt                                                         >  1:1749071/1‑100 (MQ=255)
                                      ctctAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCt                                                         >  2:3258482/1‑100 (MQ=255)
                                      ctctAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCt                                                         >  1:1735258/1‑100 (MQ=255)
                                       tctAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTa                                                        <  1:2022257/100‑1 (MQ=255)
                                        ctAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTAt                                                       >  1:671052/1‑100 (MQ=255)
                                         tAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAGGTAAAAATAATCTTAATTCGCCTAtt                                                      >  1:949521/1‑100 (MQ=255)
                                         tAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTAtt                                                      >  1:2233705/1‑100 (MQ=255)
                                         tAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTAtt                                                      >  1:912545/1‑100 (MQ=255)
                                         tAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTAtt                                                      >  1:1910068/1‑100 (MQ=255)
                                          acaaaaTATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTc                                                     >  1:2272362/3‑100 (MQ=255)
                                          aaaaaaTATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTc                                                     >  1:1968047/1‑100 (MQ=255)
                                              aaTATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAATAATCTTAATTCGCCTATTCTTtgt                                                >  2:2858695/1‑100 (MQ=255)
                                                tatCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTtgtg                                               <  1:3001660/100‑1 (MQ=255)
                                                tatCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTtgtg                                               <  1:1738879/100‑1 (MQ=255)
                                                tatCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTtgtg                                               <  1:3246782/100‑1 (MQ=255)
                                                 atCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGc                                              <  2:2720584/100‑1 (MQ=255)
                                                 accAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGc                                              <  2:2037835/98‑1 (MQ=255)
                                                  tCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGcc                                             <  2:3230673/100‑1 (MQ=255)
                                                   cAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCa                                            <  1:606431/100‑1 (MQ=255)
                                                    aGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCaa                                           >  2:2723926/1‑100 (MQ=255)
                                                    aGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCaa                                           >  2:554108/1‑100 (MQ=255)
                                                    aGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTGTGTGCCaa                                           >  2:162134/1‑100 (MQ=255)
                                                     gCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAAc                                          <  2:2327865/100‑1 (MQ=255)
                                                        caTCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTg                                       >  2:1751444/1‑100 (MQ=255)
                                                         aTCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGc                                      >  1:1338518/1‑100 (MQ=255)
                                                          tCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGcc                                     <  2:1980149/100‑1 (MQ=255)
                                                          tCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGcc                                     <  1:3227460/100‑1 (MQ=255)
                                                          tCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGcc                                     <  1:1295673/100‑1 (MQ=255)
                                                           cAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGcc                                     <  1:1252951/100‑1 (MQ=255)
                                                           cAAGATTGGTCGATAGGCTACAAAAAAAAAAAAAAAAAAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGcc                                     <  2:3287616/99‑1 (MQ=255)
                                                            acgATTGGTCGCTAGGCTACACATTAATGAAGACAGCGAAAAGCAAGTGACTGAAAGTAAAAATAATCTTAATTCGCTTATTCTTTGTGCCAATCTGcc                                     >  2:1506424/3‑99 (MQ=255)
                                                            aaGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAg                                   >  2:2990593/1‑100 (MQ=255)
                                                            aaGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAg                                   <  1:1301347/100‑1 (MQ=255)
                                                             aGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGt                                  >  1:3018336/1‑100 (MQ=255)
                                                              gATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTa                                 <  1:3095239/100‑1 (MQ=255)
                                                              gATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTa                                 >  1:2158066/1‑100 (MQ=255)
                                                                ttGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAAc                               >  1:1863158/1‑100 (MQ=255)
                                                                  tccgatctggCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAg                             <  1:1382905/92‑1 (MQ=255)
                                                                  ggTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAg                             >  1:837587/1‑100 (MQ=255)
                                                                    tCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAggg                           <  1:57302/100‑1 (MQ=255)
                                                                    tCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAggg                           >  1:507166/1‑100 (MQ=255)
                                                                      gATAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGAt                         <  2:3270441/100‑1 (MQ=255)
                                                                        tAGGCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCg                       <  1:3114407/100‑1 (MQ=255)
                                                                          ggCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCa                     >  1:240492/1‑100 (MQ=255)
                                                                          ggCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCa                     >  1:651955/1‑100 (MQ=255)
                                                                          ggCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATAGCa                     >  1:1493373/1‑100 (MQ=255)
                                                                          ggCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGAGATCgga                     >  2:1382901/1‑98 (MQ=255)
                                                                           gCTACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCaa                    >  1:1510345/1‑100 (MQ=255)
                                                                             tACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATg                  <  1:2456464/100‑1 (MQ=255)
                                                                             tACACATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATg                  >  2:124908/1‑100 (MQ=255)
                                                                              acacATTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATgg                 <  1:3010457/100‑1 (MQ=255)
                                                                               cacaTTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGc                >  2:1637531/1‑100 (MQ=255)
                                                                                acaTTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCa               >  1:220493/1‑100 (MQ=255)
                                                                                 caTTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCAt              >  1:101560/1‑100 (MQ=255)
                                                                                 caTTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCAt              >  2:2591585/1‑100 (MQ=255)
                                                                                 caTTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAACTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCAt              <  2:794755/100‑1 (MQ=255)
                                                                                  aTTAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCAtt             <  2:2279342/100‑1 (MQ=255)
                                                                                   ttAATGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCAttt            <  2:2605241/100‑1 (MQ=255)
                                                                                     aaTGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGc          <  1:1640034/100‑1 (MQ=255)
                                                                                       tGAAGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTa        >  1:3328154/1‑100 (MQ=255)
                                                                                         aaGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTaca      <  2:753125/100‑1 (MQ=255)
                                                                                         aaGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTaca      >  2:2571049/1‑100 (MQ=255)
                                                                                          aGACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTacac     <  2:2819668/100‑1 (MQ=255)
                                                                                           gACAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACAcc    <  1:2723093/100‑1 (MQ=255)
                                                                                             cAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCaa  <  2:3108608/100‑1 (MQ=255)
                                                                                             cAGCGAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCa   >  1:1145849/1‑99 (MQ=255)
                                                                                                  |                                                                                               
CAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAA  >  NC_000913/2481799‑2481992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: