BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,379,266	(C)_7→8	coding (732/1128 nt)	zapE ←	divisome ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,379,259	1	.	C	93.5%	462.3 / 24.0	138	coding (739/1128 nt)	zapE	divisome ATPase
Reads supporting (aligned to +/- strand): ref base . (4/5); new base C (61/68); total (65/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 156 total aligned reads displayed.

CGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTT‑‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAG  >  NC_000913/3379160‑3379355

cGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑ttt                                                                                                     >  2:380412/1‑100 (MQ=255)

 gCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTT‑‑c                                                                                                  <  2:1278180/100‑1 (MQ=255)

 gCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTT‑‑c                                                                                                  <  2:2376677/100‑1 (MQ=255)

   acaGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTT‑‑ccc                                                                                                >  1:287388/1‑100 (MQ=255)

     aGCGTAGTAAAAGATACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTT‑‑ccc‑cc                                                                                             <  2:1103976/100‑1 (MQ=255)

      gCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTT‑‑ccc‑ccc                                                                                            <  2:45726/100‑1 (MQ=255)

      gCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTT‑‑ccc‑ccc                                                                                            >  2:1682916/1‑100 (MQ=255)

           gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTT‑‑ccc‑cccccgcc                                                                                       <  1:1711249/100‑5 (MQ=255)

           gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTT‑‑ccc‑cccccgcc                                                                                       >  1:730620/1‑96 (MQ=255)

           gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTT‑‑ccc‑cccccgc                                                                                        >  2:1605578/1‑96 (MQ=255)

            taAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTT‑‑ccc‑cccccgcca                                                                                      >  2:2539098/1‑95 (MQ=255)

            taAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTT‑‑ccc‑cccccgcca                                                                                      >  1:2100629/1‑95 (MQ=255)

             aaaaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTT‑‑ccc‑cccccgcc                                                                                       >  2:1138495/1‑94 (MQ=255)

              aaaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAgc                                                                                     >  1:2107732/1‑100 (MQ=255)

                   gaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTACCGTCGGTGAATCTTCTCG‑TTT‑‑ccc‑cccccgccaac                                                                                    >  2:3346941/1‑88 (MQ=255)

                   gaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCGGCGCCaa                                                                                >  1:3103470/1‑100 (MQ=255)

                   gaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCaa                                                                                >  1:1677983/1‑100 (MQ=255)

                    aCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAAc                                                                               <  1:27141/100‑1 (MQ=255)

                     ccGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTT‑‑CCC‑CCCCGCCAGCGCCAAcc                                                                              <  2:2048453/100‑1 (MQ=255)

                     ccGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAAcc                                                                              <  1:956910/100‑1 (MQ=255)

                     ccGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAAcc                                                                              <  2:1473451/100‑1 (MQ=255)

                     ccGCCAGCGTCGGGTTCTCGGCGCCCATTGTGGCTAATGGCCGATGGTTGATTTCTAACGTCGGTAAATTTTCTCG‑TTCC‑CCC‑CCCCGCCAGCGCCAAc                                                                               <  2:1094740/99‑1 (MQ=255)

                       gCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCAt                                                                            >  1:1892612/1‑100 (MQ=255)

                         cAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAg                                                                          <  2:1366984/100‑1 (MQ=255)

                          aGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGt                                                                         >  2:1325689/1‑100 (MQ=255)

                          aGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGt                                                                         >  2:2852708/1‑100 (MQ=255)

                           gCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGtt                                                                        >  1:408313/1‑100 (MQ=255)

                             gTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTa                                                                      <  1:1072371/100‑1 (MQ=255)

                              tCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTCCCCC‑CCCCGCCAGCGCCAACCATAGTTTa                                                                      >  1:2732457/1‑100 (MQ=255)

                                 ggTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCa                                                                  <  2:161611/100‑1 (MQ=255)

                                 ggTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCa                                                                  <  2:1948917/100‑1 (MQ=255)

                                 ggTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCa                                                                  >  1:584090/1‑100 (MQ=255)

                                 ggTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCa                                                                  >  2:2139294/1‑100 (MQ=255)

                                   ttCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCAtt                                                                >  1:3137195/1‑100 (MQ=255)

                                     ctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTg                                                              >  2:774623/1‑100 (MQ=255)

                                     ctcGACGCCCATTGTTGCTAATGGCCGATAGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTT‑‑CCCGCCCCGCCAGCGCCAACCATAGTTTATCCATTTg                                                              <  2:2023322/100‑1 (MQ=255)

                                       cGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTgcg                                                            <  1:2329747/100‑1 (MQ=255)

                                       cGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTgcg                                                            <  2:2678537/100‑1 (MQ=255)

                                         aCGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGcc                                                          <  2:1154700/100‑1 (MQ=255)

                                          cGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGccc                                                         >  1:2767597/1‑100 (MQ=255)

                                            cccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCgg                                                       >  1:449166/1‑100 (MQ=255)

                                             ccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCggg                                                      >  2:2949381/1‑100 (MQ=255)

                                             ccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCggg                                                      <  1:127809/100‑1 (MQ=255)

                                             ccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCggg                                                      <  1:921958/100‑1 (MQ=255)

                                              cATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGt                                                     >  1:1282518/1‑100 (MQ=255)

                                                ttgttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGttt                                                   <  2:372424/100‑1 (MQ=255)

                                                  gttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCa                                                 >  1:342272/1‑100 (MQ=255)

                                                   ttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCAt                                                >  2:1854617/1‑100 (MQ=255)

                                                     gCTAATGGTCGATGGTTGATTTCTAACGTCGGAGAATTTGCTCG‑TTT‑‑CCCGCCCCTCCCGGGCCAACCATAGTTTATTCATTTTCGCCCGGGGTTc                                                  >  2:3189395/1‑96 (MQ=255)

                                                      cTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGt                                             >  1:1865643/1‑100 (MQ=255)

                                                      cTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGt                                             >  1:1076459/1‑100 (MQ=255)

                                                        aaTGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGa                                           >  1:2401858/1‑100 (MQ=255)

                                                        aaTGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGa                                           >  1:3339531/1‑100 (MQ=255)

                                                        aaTGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTC‑‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGaa                                          >  2:1847661/1‑100 (MQ=255)

                                                          tGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAg                                         <  1:1344478/100‑1 (MQ=255)

                                                           ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGt                                        >  1:477745/1‑100 (MQ=255)

                                                           ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGt                                        >  2:2310750/1‑100 (MQ=255)

                                                            gCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg                                       <  1:740831/100‑1 (MQ=255)

                                                            gCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg                                       <  1:1774124/100‑1 (MQ=255)

                                                             ccGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg                                      >  1:455681/1‑100 (MQ=255)

                                                             ccGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg                                      >  1:534151/1‑100 (MQ=255)

                                                             ccGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg                                      >  2:1695076/1‑100 (MQ=255)

                                                              cGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGc                                     >  2:604045/1‑100 (MQ=255)

                                                               gATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCg                                    <  1:3102009/100‑1 (MQ=255)

                                                                aTGGTTGATTTCTACCGCCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAg                                         <  2:730619/94‑1 (MQ=255)

                                                                  ggTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGaaa                                 <  2:1317798/100‑1 (MQ=255)

                                                                   gTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAg                                <  2:413951/100‑1 (MQ=255)

                                                                    ttGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGc                               >  1:753974/1‑100 (MQ=255)

                                                                    ttGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGc                               <  1:2373293/100‑1 (MQ=255)

                                                                    ttGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGc                               <  2:1447551/100‑1 (MQ=255)

                                                                     tGATTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGcc                              <  1:2028118/100‑1 (MQ=255)

                                                                       aTTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcac                            <  1:1370592/100‑1 (MQ=255)

                                                                       aTTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcac                            >  1:2593200/1‑100 (MQ=255)

                                                                       aTTTCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcac                            <  1:2451994/100‑1 (MQ=255)

                                                                        tttCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcaca                           >  2:2666014/1‑100 (MQ=255)

                                                                         ttCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAg                          <  1:3233828/100‑1 (MQ=255)

                                                                          tCTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGa                         >  2:2926496/1‑100 (MQ=255)

                                                                           cTAACGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGAt                        <  1:2587601/100‑1 (MQ=255)

                                                                               cGTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAACCACAGATgcgc                    >  2:3218590/1‑100 (MQ=255)

                                                                                gTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGcc                   <  1:1874796/100‑1 (MQ=255)

                                                                                gTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGcc                   <  2:2729387/100‑1 (MQ=255)

                                                                                gTCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGcc                   <  1:3351748/100‑1 (MQ=255)

                                                                                 tCGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCt                  >  1:2821156/1‑100 (MQ=255)

                                                                                  cGGTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtg                 <  1:2678491/100‑1 (MQ=255)

                                                                                   ggTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAATCCACAGATGCGCCt                  >  2:1967906/1‑98 (MQ=255)

                                                                                    gTGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag               >  2:2744526/1‑100 (MQ=255)

                                                                                    gTGAATTTTCTCG‑TTT‑‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagt              >  1:2617433/1‑100 (MQ=255)

                                                                                     tGAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagt              >  1:2377890/1‑100 (MQ=255)

                                                                                      gAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtg             <  2:2100620/100‑1 (MQ=255)

                                                                                      gAATTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtg             <  2:2760436/100‑1 (MQ=255)

                                                                                       aaTTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtg             >  2:1877274/1‑99 (MQ=255)

                                                                                        aTTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGGAGTGGCGAAAGCCACAGATGCGCCtgagtgag           <  2:2106261/100‑1 (MQ=255)

                                                                                        aTTTTCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag           >  1:3293243/1‑100 (MQ=255)

                                                                                         ttttCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag           <  2:462898/99‑1 (MQ=255)

                                                                                          tttCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGagtgagtg         <  1:1605951/100‑1 (MQ=255)

                                                                                          tttCTCG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGagtgagtg         >  2:1263042/1‑100 (MQ=255)

                                                                                            tctcG‑TTTC‑CCC‑CCCCGCCGGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTa       >  2:3245506/1‑100 (MQ=255)

                                                                                               cG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc    <  2:584089/100‑1 (MQ=255)

                                                                                               cG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc    <  1:180764/100‑1 (MQ=255)

                                                                                               cG‑TTTC‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc    <  2:753972/100‑1 (MQ=255)

CGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑TTT‑‑CCC‑CCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAG  >  NC_000913/3379160‑3379355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`