BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,606,330	(G)_8→9	noncoding (72/87 nt)	leuQ ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,322	1	.	G	80.3%	349.4 / 86.4	122	noncoding (80/87 nt)	leuQ	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base . (11/13); new base G (53/45); total (64/58)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 141 total aligned reads displayed.

CCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GA‑GG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAA  >  NC_000913/4606223‑4606421

 ccccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GA‑g                                                                                                        <  2:217148/100‑1 (MQ=255)

    cACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGT‑GA‑gg‑gg                                                                                                    >  1:2135483/1‑100 (MQ=255)

    cACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GA‑gg‑gg                                                                                                    <  1:2902444/100‑1 (MQ=255)

      cGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GA‑gg‑ggg‑g                                                                                                 >  2:1310030/1‑100 (MQ=255)

       gTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GA‑gg‑ggg‑g‑g                                                                                               >  1:1009796/1‑100 (MQ=255)

        tCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GA‑gg‑ggg‑g‑gg                                                                                              <  1:2800531/100‑1 (MQ=255)

          cGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GA‑gg‑ggg‑g‑ggga                                                                                            >  2:480662/1‑98 (MQ=255)

          cGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GA‑gg‑ggg‑g‑ggga                                                                                            >  1:2885582/1‑98 (MQ=255)

          cGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTAGTTTTTGGTGC‑GA‑gg‑ggt‑g‑ggga                                                                                            >  1:3140629/1‑98 (MQ=255)

           gTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GA‑gg‑ggg‑g‑gggac                                                                                           <  2:1185562/100‑4 (MQ=255)

             aaGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCACTGCCAGCACTAACAACGTGGTTCTTCCTCC‑GA‑gg‑cgc‑g‑ggctctt                                                                                         >  2:349003/1‑95 (MQ=17)

              aGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GA‑gg‑ggg‑g‑ggggactt                                                                                        <  1:1893975/100‑7 (MQ=255)

               gAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCACCGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGa                                                                                       >  1:2858008/1‑100 (MQ=255)

                aaCACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGaa                                                                                      <  1:890986/100‑1 (MQ=255)

                 acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAAc                                                                                     <  1:1922372/100‑1 (MQ=255)

                  cacTAACACCTGACGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAAcc                                                                                    <  2:559839/100‑1 (MQ=255)

                  cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAAcc                                                                                    <  1:1237849/100‑1 (MQ=255)

                  cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAAcc                                                                                    <  1:1978113/100‑1 (MQ=255)

                   acTAACACCTTACGGTAGCGCGTCTACCAATTCCGCCACCTTCGCCGACTGCCAGCAATACCAACGTGATTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAAcc                                                                                    <  1:1826436/99‑1 (MQ=21)

                   acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAAccc                                                                                   <  1:64473/100‑1 (MQ=255)

                    ccgaggccTGAGTCTAGCGGGGCTACCTGTTCCGGCACCTTCGCGCTGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAAcccc                                                                                  <  1:3027859/94‑1 (MQ=255)

                    cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGa                                                                                       <  2:2644002/95‑1 (MQ=255)

                     tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCGCCTTCGCCCAGTGCGAGCAATATAAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAAccccc                                                                                 <  1:2454567/100‑1 (MQ=37)

                     tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GA‑GG‑GGG‑G‑GG‑ACTTGAACCCCCa                                                                                >  1:401706/1‑100 (MQ=255)

                      aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAAccccaa                                                                                <  1:2273050/100‑3 (MQ=255)

                      aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCa                                                                                <  1:478201/100‑1 (MQ=255)

                      aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCa                                                                                >  1:610748/1‑100 (MQ=255)

                      aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGAGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCa                                                                                >  1:2352202/1‑100 (MQ=255)

                       acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCAc                                                                               <  1:446827/100‑1 (MQ=255)

                       acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCACTGCGAGCTATTAAAACGTCGTAATTGCTTC‑GA‑GG‑GGG‑G‑GGAACTTGAAc                                                                                     >  2:1620502/1‑94 (MQ=12)

                        cacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACg                                                                              <  1:387584/100‑1 (MQ=255)

                         acCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGt                                                                             >  1:1291769/1‑100 (MQ=255)

                         acCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGt                                                                             >  1:280324/1‑100 (MQ=255)

                         acCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGt                                                                             >  1:1289087/1‑100 (MQ=255)

                          ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTc                                                                            >  1:387241/1‑100 (MQ=255)

                           cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCAACGTAGTGCTTCGTCC‑GA‑GGCGCG‑T‑GG‑TATTGAACCCCCActt                                                                             >  2:1405625/1‑96 (MQ=12)

                           cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTc                                                                            >  1:859257/1‑99 (MQ=255)

                            tGAAGCTAAAGCTTATACAAATACCACCACCTTCGCCAAATGCGAGCAATATCAACGTGGTTTTTGGTGC‑GA‑GG‑GGG‑G‑GG‑ACTTGAACCCCCAc                                                                               <  2:863437/94‑1 (MQ=25)

                             gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGt                                                                         <  1:1364171/100‑1 (MQ=255)

                              aggCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTa                                                                        <  1:1754290/98‑1 (MQ=255)

                              aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGAGCGAGCAATATAAACGTGGTATTTGGTGC‑GA‑GG‑GGG‑A‑GG‑ACTTTAACACCCACATctttaa                                                                       >  2:2141687/1‑95 (MQ=25)

                                gCTAGCGCGTCTACCAATTCCGCCCCCTTCGCCCAGTTCGAGCAATATCAACGTGGTTTTTGGTGC‑GA‑GG‑GGGCG‑TG‑ACTTGAACCCCCACGTCCGTAAg                                                                      <  1:1668071/100‑1 (MQ=38)

                                 cTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GA‑GG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGa                                                                    <  1:299024/100‑1 (MQ=255)

                                 cTAGCGCCTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAgg                                                                     <  1:3010729/100‑1 (MQ=255)

                                  tAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GA‑GGCGGG‑G‑GG‑CCTTGAACCCCCACTTCCGTAAGGa                                                                    >  2:1357024/1‑100 (MQ=38)

                                   aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGac                                                                   <  1:1169196/100‑1 (MQ=255)

                                   aGCGCGTCTACCAATCCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGac                                                                   <  1:953106/100‑1 (MQ=255)

                                    gcgcGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGT‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGaca                                                                  <  2:2135473/100‑1 (MQ=255)

                                     cgcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGacac                                                                 >  1:1015097/1‑100 (MQ=255)

                                        gTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTaa                                                              >  2:1139067/1‑100 (MQ=255)

                                          cTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAaca                                                            >  1:3125056/1‑100 (MQ=255)

                                           tACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAacac                                                           >  1:2064632/1‑100 (MQ=255)

                                           tACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GA‑GG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACAcc                                                          >  1:1616659/1‑100 (MQ=255)

                                            aCCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑TCTTGAACCCCCACGTCCGTAAGGACACTAACAcc                                                          >  2:1633578/1‑100 (MQ=255)

                                            aCCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACAcc                                                          >  1:2234887/1‑100 (MQ=255)

                                            aCCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACAcc                                                          <  1:1206205/100‑1 (MQ=255)

                                            aCCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTCTTGGTGC‑GA‑GG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCt                                                         >  2:969670/1‑100 (MQ=255)

                                             ccAATTCCGCCCCCTTCGCCCAGTGCGTGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCt                                                         <  1:550714/100‑1 (MQ=25)

                                             ccAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCt                                                         >  2:827418/1‑100 (MQ=255)

                                             ccAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACACCCACGTCCGTAAGGACACt                                                                >  2:2551590/1‑93 (MQ=255)

                                              cAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTg                                                        <  1:2669991/100‑1 (MQ=255)

                                               aaTTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGa                                                       >  1:1439510/1‑100 (MQ=255)

                                                 ttCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAg                                                     <  1:2472622/100‑1 (MQ=255)

                                                   ccGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCt                                                   >  1:2099427/1‑100 (MQ=255)

                                                   ccGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCt                                                   >  2:2271391/1‑100 (MQ=255)

                                                     gCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                                 >  2:1615641/1‑100 (MQ=255)

                                                     gCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                                 >  2:2376095/1‑100 (MQ=255)

                                                       ctccTTCGCCCAGTGGGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcg                                               <  1:1562253/98‑1 (MQ=21)

                                                         ccTTCGCCCAGTGCGAGCATTTTCAACCTCGTTTTTGGTGCGGG‑GG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcg                                               <  2:2414115/98‑1 (MQ=18)

                                                         ccTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GA‑GG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGt                                            >  2:2472059/1‑100 (MQ=255)

                                                             cGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa                                         <  1:681145/100‑1 (MQ=255)

                                                              gCCCAGTGCGGGCAATATCAACTTGGTTTTTGGGGCGGG‑GG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa                                         <  1:255393/99‑1 (MQ=17)

                                                               cccAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAcc                                       >  1:912860/1‑100 (MQ=255)

                                                               cccAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAcc                                       <  1:28834/100‑1 (MQ=255)

                                                               cccAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGATAGCGCGTCTAcc                                       >  1:1955521/1‑100 (MQ=255)

                                                                ccATTGCGAGCAACATCAACCTCGTTTTTGGTTC‑GA‑GG‑GGG‑GTGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                                      <  2:2352191/100‑1 (MQ=17)

                                                                    tGCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                                  >  1:134885/1‑100 (MQ=255)

                                                                     gCGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                 >  2:2918404/1‑100 (MQ=255)

                                                                      cGAGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCATGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                >  2:474539/1‑100 (MQ=255)

                                                                      cGAGCAATATCAACGGGGTTGTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                <  1:1781719/100‑1 (MQ=17)

                                                                        aGCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                              >  1:1308710/1‑100 (MQ=255)

                                                                         gCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                             >  2:3319419/1‑100 (MQ=255)

                                                                         gCAATATCAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                             >  1:2651420/1‑100 (MQ=255)

                                                                                cAACGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc                      >  2:2028586/1‑100 (MQ=255)

                                                                                cAACGTGGTTTTTGATGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc                      <  2:280323/100‑1 (MQ=255)

                                                                                  aCGTGGTTTTTGGTGC‑GA‑GG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATa                   <  1:1376401/100‑1 (MQ=255)

                                                                                   cGTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCaaa                   <  1:2271402/100‑3 (MQ=255)

                                                                                    gcctcgtttGGTGC‑GA‑GG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGATCGGAAAATCCGCCACCTTGGCaaa                   >  2:1562249/7‑96 (MQ=14)

                                                                                    gTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑CCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATAc                  >  2:1427665/1‑100 (MQ=255)

                                                                                    gTGGTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATAc                  >  1:2958691/1‑100 (MQ=255)

                                                                                       gTTTTTTGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTCAAGCTAGCGCGGCTACCAATTCCGCCACCTTCGCATACCAt               <  2:759333/100‑1 (MQ=14)

                                                                                       gTTTTTGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCAt               >  1:1384142/1‑100 (MQ=255)

                                                                                        tttttGGTGCGGG‑GG‑GGG‑G‑GG‑ACTTGATCCCCCACGTCCGTCAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCAt               <  2:2064624/99‑1 (MQ=14)

                                                                                        tttttGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATc              >  2:1370714/1‑100 (MQ=255)

                                                                                         ttttGGTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCa             >  2:1755844/1‑100 (MQ=255)

                                                                                              ntGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCt        >  1:1708501/2‑100 (MQ=255)

                                                                                              gTGC‑GAGGG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCt        >  1:913124/1‑100 (MQ=255)

                                                                                              gTGC‑GA‑GG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTCAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCt        <  2:1563056/99‑1 (MQ=255)

                                                                                                   aa‑GG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaa    <  1:494949/98‑1 (MQ=255)

                                                                                                    a‑GG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaaaa  <  1:2472070/100‑1 (MQ=255)

CCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC‑GA‑GG‑GGG‑G‑GG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAA  >  NC_000913/4606223‑4606421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`