Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,067,3230AT21.3% 286.7 / 72.8 128intergenic (‑150/+17)ygfI/yggEputative DNA‑binding transcriptional regulator/oxidative stress defense protein
Reads supporting (aligned to +/- strand):  ref base A (60/40);  new base T (15/12);  total (76/52)
Fisher's exact test for biased strand distribution p-value = 8.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

Only 100 of 132 total aligned reads displayed.

TGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAA  >  NC_000913/3067224‑3067422
                                                                                                   |                                                                                                   
tgatgaAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATa                                                                                                     >  2:2837835/1‑100 (MQ=255)
tgatgaAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATa                                                                                                     >  1:712481/1‑100 (MQ=255)
   tgaAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGt                                                                                                  >  2:2997840/1‑100 (MQ=255)
   tgaAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGt                                                                                                  >  1:3095716/1‑100 (MQ=255)
     aaaCCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCg                                                                                                <  2:3095699/100‑1 (MQ=255)
      aaCCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCgg                                                                                               >  2:3304921/1‑100 (MQ=255)
      aaCCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCgg                                                                                               <  2:1477375/100‑1 (MQ=255)
      aaCCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCg                                                                                                >  2:2965154/1‑99 (MQ=255)
       aCCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCggn                                                                                              <  1:2827814/100‑2 (MQ=255)
        ccGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGc                                                                                             <  1:2522713/100‑1 (MQ=255)
        ccGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGc                                                                                             <  1:1549784/100‑1 (MQ=255)
        ccGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGc                                                                                             <  1:319501/100‑1 (MQ=255)
        ccGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGc                                                                                             >  1:1345839/1‑100 (MQ=255)
        ccGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGc                                                                                             >  2:940569/1‑100 (MQ=255)
         cgcgAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGCt                                                                                            <  1:2825765/100‑1 (MQ=255)
         cgagAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGCt                                                                                            <  2:370561/97‑1 (MQ=255)
          gcgAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGCTa                                                                                           <  1:1514232/100‑1 (MQ=255)
          gcgAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGCTa                                                                                           <  1:652569/100‑1 (MQ=255)
           cgAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTAc                                                                                          <  2:594103/100‑1 (MQ=255)
           cgAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCAGTAGCCCGACGTATTCGTCGGGCTaa                                                                                          <  1:1935662/100‑2 (MQ=39)
            gAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGCTaat                                                                                         <  2:1071422/100‑3 (MQ=255)
              gCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACa                                                                                         >  1:1337256/1‑98 (MQ=255)
                  aGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAAtatt                                                                                   <  1:1331026/100‑1 (MQ=255)
                  aGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAAtatt                                                                                   >  1:398884/1‑100 (MQ=255)
                    aTAGGTGATTCCGTCATGGTTTTACAGAAAATTCTAGAAACAGGACGCAATATCGAGTAAAGGCATTAGCCCGCCGAATACGTCGGGCTACAAAcaatat                                                                                 <  2:2002455/100‑1 (MQ=255)
                    aTAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAAtattat                                                                                 <  2:3089532/100‑1 (MQ=255)
                       ggTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATtgt                                                                              >  1:373877/1‑100 (MQ=255)
                       ggTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATtgt                                                                              >  2:199081/1‑100 (MQ=255)
                        gTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATtgtg                                                                             >  2:884027/1‑100 (MQ=255)
                         tGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGtgc                                                                            >  1:3214715/1‑100 (MQ=255)
                          gATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGtgct                                                                           >  1:1180007/1‑100 (MQ=255)
                            ttACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGtgctgc                                                                         >  2:721430/1‑100 (MQ=255)
                            ttACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGtgctgc                                                                         >  1:69810/1‑100 (MQ=255)
                             tACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATCTTATTGTGCTGCa                                                                        <  2:2075903/100‑1 (MQ=255)
                             tACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCa                                                                        <  1:2943300/100‑1 (MQ=255)
                              aCGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAg                                                                       <  1:2844912/100‑1 (MQ=255)
                               cGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATTCGTCGGGCTACAAATATGATTGTGCTGCatg                                                                      <  1:2260307/100‑3 (MQ=255)
                                gTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGgt                                                                     >  1:3269631/1‑100 (MQ=255)
                                 tCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGgtg                                                                    >  1:235266/1‑100 (MQ=255)
                                 tCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGgtg                                                                    <  2:1548225/100‑1 (MQ=255)
                                  cATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGgtgt                                                                   >  2:2467490/1‑100 (MQ=255)
                                   aTGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAACTATTATTGTGCTGCAGgtgt                                                                   >  1:2829100/1‑99 (MQ=255)
                                    tGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGttt                                                                 >  1:1104635/1‑100 (MQ=255)
                                    tGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAAGATTATTGTGCTGCAGGTGttt                                                                 >  1:1832313/1‑100 (MQ=255)
                                     ggTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGtttt                                                                >  1:1075623/1‑100 (MQ=255)
                                     ggTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGtttt                                                                <  1:1441324/100‑1 (MQ=255)
                                      gTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTa                                                               >  1:1753707/1‑100 (MQ=255)
                                        tttACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGc                                                             >  2:723859/1‑100 (MQ=255)
                                           acagaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCggg                                                          >  1:2074513/1‑100 (MQ=255)
                                            cagaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGgt                                                         >  2:163466/1‑100 (MQ=255)
                                            cagaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATAGTATTGTGCTGCAGGTGTTTTAGCGGgt                                                         >  2:2118639/1‑100 (MQ=255)
                                             agaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGgtt                                                        >  2:1002214/1‑100 (MQ=255)
                                             agaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGgtt                                                        >  1:3088387/1‑100 (MQ=255)
                                                aaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGgttgtt                                                     >  2:1431407/1‑100 (MQ=255)
                                                 aattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGgttgtt                                                     <  2:671982/99‑1 (MQ=255)
                                                  attacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGa                                                   <  1:1013622/100‑1 (MQ=255)
                                                     acagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATcc                                                <  2:1870967/100‑1 (MQ=255)
                                                     acagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATcc                                                <  2:2268286/100‑1 (MQ=255)
                                                     acagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATcc                                                >  1:3113283/1‑100 (MQ=255)
                                                      cagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCca                                               >  2:1715336/1‑100 (MQ=255)
                                                        gaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCcaca                                             >  2:1740807/1‑100 (MQ=255)
                                                        gaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCcaca                                             >  1:1252389/1‑100 (MQ=255)
                                                        gaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCcaca                                             >  2:157994/1‑100 (MQ=255)
                                                              ggaggCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCt                                       >  2:1050872/1‑100 (MQ=255)
                                                               gaggCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTa                                      >  1:2214933/1‑100 (MQ=255)
                                                                aggCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTaa                                     >  1:159323/1‑100 (MQ=255)
                                                                   cAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTg                                  >  1:859020/1‑100 (MQ=255)
                                                                    aaTATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTgg                                 <  2:1337252/100‑1 (MQ=255)
                                                                     atatCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGa                                <  1:939435/100‑1 (MQ=255)
                                                                       ntCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAg                              >  2:93110/2‑100 (MQ=255)
                                                                          gggTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATGGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGCTCTAACTGGAAGa                             >  2:2889102/1‑98 (MQ=255)
                                                                           ggTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACca                          <  2:3214697/100‑1 (MQ=255)
                                                                           ggTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACca                          >  1:1017676/1‑100 (MQ=255)
                                                                             tAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACcaca                        >  2:2814464/1‑100 (MQ=255)
                                                                              aaaGGCATTAGCCCGACGAGTCCGACGGGCTAAAAATATTATTGTGATGCACGTGTCGTAGCAATCTGTTGATTTACAGGCTCGAACTGGAAGAcaa                          >  2:2684238/1‑95 (MQ=255)
                                                                              aaaGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACAt                       >  2:1609748/1‑100 (MQ=255)
                                                                              aaaGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTGTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACcaca                        <  2:3088370/99‑1 (MQ=255)
                                                                               gatcttgtAGCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATc                      <  1:973820/93‑1 (MQ=38)
                                                                                   cATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGAcc                  <  2:372502/100‑1 (MQ=255)
                                                                                   cATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGAcc                  >  1:2533473/1‑100 (MQ=255)
                                                                                    tgtAGCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATcagatc                 >  2:973818/3‑95 (MQ=38)
                                                                                    tgtAGCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCt                 >  1:409205/3‑100 (MQ=255)
                                                                                    tgtAGCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCt                 >  1:2230844/3‑100 (MQ=255)
                                                                                     gtAGCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTg                >  1:1639589/2‑100 (MQ=255)
                                                                                     gtAGCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTg                >  1:1290093/2‑100 (MQ=255)
                                                                                      tAGCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGa               >  1:1360116/1‑100 (MQ=255)
                                                                                       aGCCCGACGAATACGTCGGGCTACAAATATTATTATGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGa               >  2:1581710/1‑99 (MQ=255)
                                                                                        gCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatc             >  1:141045/1‑100 (MQ=255)
                                                                                        gCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatc             >  1:1558211/1‑100 (MQ=255)
                                                                                        gCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatc             >  2:2140504/1‑100 (MQ=255)
                                                                                        gCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatc             >  2:1631742/1‑100 (MQ=255)
                                                                                         cccGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatca            >  1:1665585/1‑100 (MQ=255)
                                                                                          ccGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatcat           >  1:2119527/1‑100 (MQ=255)
                                                                                          ccGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatcat           >  1:1824157/1‑100 (MQ=255)
                                                                                          ccGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatcat           >  1:2863272/1‑100 (MQ=255)
                                                                                             acgaATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCaa        <  2:69809/100‑1 (MQ=255)
                                                                                                aaTACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACt     <  2:1075622/100‑1 (MQ=255)
                                                                                                 aTACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTg    >  1:2616642/1‑100 (MQ=255)
                                                                                                   aCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGaa  <  2:235265/100‑1 (MQ=255)
                                                                                                   aCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGGTGTTGTTCCACAGGTTCTAACTGGAAGAGCACATCGACCTGATCATCAAACTGa   >  2:1615709/1‑99 (MQ=255)
                                                                                                   |                                                                                                   
TGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAA  >  NC_000913/3067224‑3067422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: