Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9840TG42.1% 292.0 / inf 247intergenic (‑194/+114)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base T (76/67);  new base G (90/14);  total (166/81)
Fisher's exact test for biased strand distribution p-value = 2.32e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.96e-01

Only 100 of 252 total aligned reads displayed.

TTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑T‑‑‑T‑TCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCT  >  NC_000913/3705885‑3706083
                                                                                                   |                                                                                                          
ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                                                            <  2:2466725/100‑1 (MQ=255)
  gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                                          >  1:1427975/1‑100 (MQ=255)
       cGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCgg                                                                                                     >  1:101277/1‑100 (MQ=255)
        ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                                                    >  2:640529/1‑100 (MQ=255)
         gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                                   <  1:581583/100‑1 (MQ=255)
         gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                                   <  2:1002249/100‑1 (MQ=255)
         gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                                   <  1:377218/100‑1 (MQ=255)
         gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGca                                                                                                   <  2:2508104/100‑2 (MQ=37)
          aTGGCGCTGCACGCAGATGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGcat                                                                                                  <  1:1236862/100‑3 (MQ=25)
          aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGcat                                                                                                  <  2:520025/100‑3 (MQ=37)
          aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGcat                                                                                                  <  1:1935862/100‑3 (MQ=37)
            ggCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGCATaa                                                                                                <  1:1845536/100‑2 (MQ=25)
                 tctaCGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAAt                                                                                          <  1:1624342/97‑1 (MQ=255)
                   cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCg                                                                                        <  1:2767142/100‑1 (MQ=255)
                     cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTg                                                                                      >  1:2944069/1‑100 (MQ=255)
                        agagGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGcga                                                                                   >  2:2039708/1‑98 (MQ=255)
                        agagGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCaa                                                                                   <  1:356623/100‑1 (MQ=255)
                            gCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTGATCCGGCCTAC‑AAAATCGTGCAAATTc                                                                               <  2:2838098/100‑1 (MQ=255)
                            gCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCCTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTc                                                                               <  1:216976/100‑1 (MQ=255)
                             cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGAGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCa                                                                              >  2:1798117/1‑100 (MQ=255)
                               caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAAt                                                                            <  2:181666/100‑1 (MQ=255)
                               caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAAt                                                                            <  2:1059568/100‑1 (MQ=255)
                               caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAAt                                                                            <  2:529687/100‑1 (MQ=255)
                                 gcgcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATAc                                                                          >  2:1016575/1‑100 (MQ=255)
                                   gcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACAt                                                                        <  2:2105529/100‑1 (MQ=255)
                                    ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACAtt                                                                       >  2:3303309/1‑100 (MQ=255)
                                     cACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTg                                                                      >  2:1264582/1‑100 (MQ=255)
                                      aCCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGc                                                                     <  1:1762730/100‑1 (MQ=255)
                                        cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑g                                                                  <  1:2392299/100‑1 (MQ=255)
                                        cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATGCAATACATTGCA‑g                                                                  >  2:1510990/1‑100 (MQ=255)
                                               aCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAACTCGTGCAAATTCAATACCTTGCA‑GAAT‑T‑‑‑T‑Tc                                                      <  1:2256191/100‑1 (MQ=255)
                                                  gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑T‑‑‑T‑TCGTa                                                   >  1:1043269/1‑100 (MQ=255)
                                                   aTTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑T‑‑‑T‑TCGTAg                                                  >  2:369423/1‑100 (MQ=255)
                                                      gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑T‑‑‑T‑TCGTAGGcc                                               <  2:2773431/100‑1 (MQ=255)
                                                      gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑T‑‑‑T‑TCGTAGGcc                                               <  2:1650893/100‑1 (MQ=255)
                                                       ttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑T‑‑‑T‑TCGTAGGCCt                                              >  1:2843728/1‑100 (MQ=255)
                                                               gTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑T‑‑‑T‑TCGTAGGCCTGATAAGCg                                      >  1:1982931/1‑100 (MQ=255)
                                                                     attttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAAATCGTGCTAATTCAATATATTGCA‑GAAA‑C‑‑‑C‑TTGTAGGCCTGATAAGCGTAgcgc                                >  1:1539805/10‑100 (MQ=18)
                                                                      atttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAATTC‑TGCAAATTCAACAAATTGC‑‑GAAT‑C‑‑‑CCTTGTAGGCCTGATAAGCGTAGCGCAt                              >  1:1550453/9‑100 (MQ=18)
                                                                       tttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCATAC‑‑AAATCATGCAAATCCAAAATATTGCA‑GGAG‑T‑‑‑T‑ATGTAGGCCTGATAAGCGTAGCGCATc                             >  1:669997/8‑100 (MQ=18)
                                                                       tGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑T‑‑‑T‑TCGTAGGCCTGATAAGCGGAGCGCAt                              >  1:1736916/1‑100 (MQ=255)
                                                                        gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑T‑‑‑T‑TCGTAGGCCTGATAAGCGGAGCGCATc                             >  2:1326802/1‑100 (MQ=255)
                                                                        gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑T‑‑‑T‑TCGTAGGCCTGATAAGCGGAGCGCATc                             >  2:1169837/1‑100 (MQ=255)
                                                                        gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑T‑‑‑T‑TCGTAGGCCTGATAAGCGGAGCGCATc                             <  2:349710/100‑1 (MQ=255)
                                                                         tgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAAATCGTGCTAATTCAATATATTGCA‑GAAA‑C‑‑‑C‑TTGTAGGCCTGATAAGCGTAGCGCATCa                            >  2:2585220/6‑100 (MQ=17)
                                                                         tgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAATTGTGTAAATTCAATAAATTGAT‑CGAT‑C‑‑‑C‑ACGTAGGCCTGATAAGCGTAGCGCATCa                            >  1:2613621/6‑100 (MQ=21)
                                                                          ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑T‑‑‑T‑TCGTAGGCCTGATAAGCGGAGCGCATCAg                           >  1:1336780/1‑100 (MQ=255)
                                                                          gtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAATTC‑TGCAAATTCAACAAATTGC‑‑GAATCC‑‑‑C‑TTGTAGGCCTGATAAGCGTAGCGCATCAgg                          >  2:738645/5‑100 (MQ=18)
                                                                          gtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAAAGCATGCAAATTCAATATATTGCA‑GAGT‑T‑‑‑C‑ACGTAGGCCTGATAAGCGTAGCGCATCAg                           >  1:841995/5‑100 (MQ=17)
                                                                          gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑‑AAACCGCGCAAATTCAATATATTGCG‑GAGA‑A‑‑‑A‑ATGTAGGCCTGATAAGCGTAGCGCATCAgg                          >  1:1821335/5‑100 (MQ=18)
                                                                           taggCCGGATAAGGCGTTCACGCCGCATCCGGCATAC‑AAAGTCGTATAAATTCAATGTATTGCA‑GTTT‑A‑‑‑T‑TGGTAGGCCTGATAAGCGCAGCGCATCAgg                          >  1:3230938/4‑100 (MQ=21)
                                                                           tATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑T‑‑‑T‑TCGTAGGCCTGATAAGCGGAGCGCATCAgg                          <  1:498528/100‑1 (MQ=255)
                                                                            aggCCGGATAAGGCGTTTACGCCGCATCTGGCCTAC‑AAATTCTTCCAAATTCAATATATTGCA‑GAAA‑T‑‑‑C‑ATGTAGGCCTGATAAGCGTAGCGCATCAGGc                         >  1:119484/3‑100 (MQ=18)
                                                                            aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAAATCGTGCTAATTCAATATATTGCA‑GAAA‑C‑‑‑C‑TTGTAGGCCTGATAAGCGTAGCGCATCAGGc                         >  1:3119162/3‑100 (MQ=18)
                                                                            aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAAAATGTGCAAATTCAATAAATTGC‑‑‑AAT‑TCAAC‑TTGTAGGCCTGATAAGCGCAGCGCATCAgg                          >  2:2588132/3‑100 (MQ=21)
                                                                            aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCA‑GGAC‑C‑‑‑A‑T‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                         >  1:1277869/3‑100 (MQ=18)
                                                                            aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAGTCGTATAAATTCAATGTATTGCA‑GTTT‑A‑‑‑T‑TGGTAGGCCTGATAAGCGCAGCGCATCAGGc                         >  1:883459/3‑100 (MQ=21)
                                                                            aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAATCGCTTAAATTCAATATATTGCA‑CCAT‑C‑‑‑C‑TCGTAGGCCTGATAAGCGTAGCGCATCAGGc                         >  2:1567046/3‑100 (MQ=21)
                                                                            aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAAGCATGAAAATTCAATATATTGCA‑GGAG‑C‑‑‑T‑GCGTAGGCCTGATAAGCGTAGCGCATCAGGc                         >  2:1592886/3‑100 (MQ=18)
                                                                             tGCCGGATGCTGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGGGCAAAGTCAATACATTGCA‑GAAT‑T‑‑‑T‑TCGTAGGCCTGATAAGCGGAGCGCATCAGGCa                        >  2:2675484/1‑100 (MQ=255)
                                                                             tGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑T‑‑‑T‑TCGTAGGCCTGATAAGCGGAGCGCATCAGGCa                        >  1:1370537/1‑100 (MQ=255)
                                                                             ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAAATCGTGCTAATTCAATATATTGCA‑GAAA‑C‑‑‑C‑TTGTAGGCCTGATAAGCGTAGCGCATCAGGCa                        >  2:1890783/2‑100 (MQ=17)
                                                                             ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAAACAGTGCAAATTCAATATATTGCA‑ATCT‑T‑‑‑C‑TGGTAGGCCTGATAAGCATCGCGCATCAGGCa                        >  1:2145711/2‑100 (MQ=18)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAGATCGTGCAAATTCAATATATTGCA‑TGAG‑C‑‑‑C‑ACGTAGGCCTGATAAGCGTAGCGCATCAGGCa                        >  1:2287294/2‑100 (MQ=17)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAATCCTTGCGAATTCAATATATTGTATGGAA‑A‑‑‑T‑TC‑‑AGGCCTGATAAGCGTAGCGCATCAGGctt                       >  2:1444908/2‑98 (MQ=21)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAATCGCTTAAATTCAATATATTGCA‑CCAT‑C‑‑‑C‑TCGTAGGCCTGATAAGCGTAGCGCATCAGGCa                        >  1:3014478/2‑100 (MQ=21)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAACCTTGCTAATTCAATATATTGCA‑GGGA‑C‑‑‑T‑ATGTAGGCCTGATAAGCATAGCGCATCAGGCa                        >  1:1928235/2‑100 (MQ=18)
                                                                             ggCCGGATAAGGCGTTAACGCCGCATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑T‑‑‑T‑TCGTAGGCCTGATAAGCGGAGCGCATCAGGCa                        >  2:596407/2‑100 (MQ=17)
                                                                              gctggataaggcgTTCACGCCGCATCCAGCCTAC‑AAAATTGTGCAAAATCAATGGATTGCA‑CAGC‑T‑‑‑A‑GCGTAGGCCTGATAAGCGTAGCGCATCAGGCag                       >  2:1240779/10‑99 (MQ=25)
                                                                              gCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑T‑‑‑T‑TCGTAGGCCTGATAAGCGGAGCGCATCAGGCAt                       >  1:2021114/1‑100 (MQ=255)
                                                                              gCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAATTC‑TGCAAATTCAACAAATTGC‑‑GAATCC‑‑‑C‑TTGTAGGCCTGATAAGCGTAGCGCATCAGGCAtt                      >  1:3315229/1‑100 (MQ=18)
                                                                              gCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAAATCGTGCTAATTCAATATATTGCA‑GAAA‑C‑‑‑C‑TTGTAGGCCTGATAAGCGTAGCGCATCAGGCag                       >  1:2568400/1‑99 (MQ=18)
                                                                              gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCA‑GGAC‑C‑‑‑A‑T‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag                       >  2:3341620/1‑99 (MQ=18)
                                                                              gCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑‑AAATCATGCAAATCCAAAATATTGCA‑GGAG‑T‑‑‑T‑ATGTAGGCCTGATAAGCGTAGCGCATCAGGCAtt                      >  1:2497360/1‑100 (MQ=21)
                                                                              gCCGGATAAGGCGTTCACGCCGCATCCGGCATAC‑AAAATTGTGTAAATTCAATAAATTGAT‑CGAT‑C‑‑‑C‑ACGTAGGCCTGATAAGCGTAGCGCATCAGGCag                       >  1:1213618/1‑99 (MQ=21)
                                                                               ccggataaggcgTTTACGCCGCATCCGGCCTAC‑AAAATCGTGCTAATTCAATATATTGCA‑GAAA‑C‑‑‑C‑TTGTAGGCCTGATAAGCGTAGCGCATCAGGCagt                      >  2:159578/9‑98 (MQ=18)
                                                                               ccggataaggcgTTCACGCCTTATCCGGCCTAC‑AAAATCATGAAAATTCAATACATTGCA‑AGAT‑T‑‑‑T‑TCGTAGGCCTGATAAGCGTAGCGCATCAGGCacg                      >  2:2299052/9‑98 (MQ=17)
                                                                               ccggataaggcgTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCA‑GGAC‑C‑‑‑A‑T‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagt                      >  2:1717498/9‑98 (MQ=18)
                                                                               ccggataaggcgTTCACGCCGCATCCGGCCTAC‑AAAACTGTGCAAATTCAATATATTGCA‑GGAA‑A‑‑‑C‑ACGTAGGCCTGATAAGCGAAGCGCATCAGGCagt                      >  1:711442/9‑98 (MQ=18)
                                                                               ccGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAAGCATGAAAATTCAATATATTGCA‑GGAG‑C‑‑‑T‑GCGTAGGCCTGATAAGCGTAGCGCATCAGGCagt                      >  2:310193/1‑98 (MQ=17)
                                                                                 ggataaggcgTTTACGCCGCATCCGGCCTAC‑AAATTC‑TGCAAATTCAACAAATTGC‑‑GAATCC‑‑‑C‑TTGTAGGCCTGATAAGCGTAGCGCATCAGGCAtttgc                   >  1:1130178/7‑98 (MQ=21)
                                                                                 ggataaggcgTTTACGCCGCATCCGGCCTAC‑AAAATCGTGCTAATTCAATATATTGCA‑GAAA‑C‑‑‑C‑TTGTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                    >  2:3106379/7‑100 (MQ=18)
                                                                                 ggataaggcgTTTACGCCGCATCCGGCCTAC‑AAAAGCGTGCAAATTCAATACATTGCATGGGC‑C‑‑‑A‑T‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAattt                    >  2:2383002/7‑100 (MQ=18)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCTAC‑AAGATCGTGCAAATTCAATATATTGCA‑TGAG‑C‑‑‑C‑ACGTAGTCCTGATAAGCGTATCGCATAAGGCa                        >  1:1296213/7‑96 (MQ=17)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCTAC‑AAAGTCGTATAAATTCAATGTATTGCA‑GTTT‑A‑‑‑T‑TGGTAGGCCTGATAAGCGCAGCGCATCAGGCaatct                    >  2:2172710/7‑96 (MQ=21)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCTAC‑AAAGTCGTATAAATTCAATGTATTGCA‑GTTT‑A‑‑‑T‑TGGTAGGCCTGATAAGCGCAGCGCATCAGGCaatct                    >  2:1628485/7‑96 (MQ=25)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCTAC‑AAAATTGTGTAAATTCAATAAATTGAT‑CGAT‑C‑‑‑C‑ACGTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                    >  1:411605/7‑100 (MQ=21)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCTAC‑AAAACCTTGCTAATTCAATATATTGCA‑GGGA‑C‑‑‑T‑ATGTAGGCCTGATAAGCATAGCGCATCAGGCagctt                    >  1:749690/7‑96 (MQ=18)
                                                                                  gataaggcgTTCACGCCGCATCCGGCCTAC‑AAAAGCACGCAAACTCAATATATTGCA‑GAGA‑T‑‑‑C‑ATGTAGGCCTGATAAGCGTAGCGCATCAGGCAattta                   >  2:975239/6‑99 (MQ=17)
                                                                                     ttccgatctaCGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑T‑‑‑T‑TCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcc                <  1:2207786/91‑1 (MQ=255)
                                                                                     gcggcgTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GACT‑T‑‑‑T‑TCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcc                >  2:783277/1‑100 (MQ=255)
                                                                                     gcggcgTTAACAGGGTATCCGGCTTAT‑AATATCGTGCAAATTCAAGACATTGCA‑GAAT‑T‑‑‑T‑TCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgc                 <  2:3032259/99‑1 (MQ=18)
                                                                                         cgTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑T‑‑‑T‑TCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttg            <  1:1103449/100‑1 (MQ=255)
                                                                                          gTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑T‑‑‑T‑TCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgn           <  1:3070430/100‑2 (MQ=255)
                                                                                              aCGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑T‑‑‑T‑TCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATc       >  1:1587040/1‑100 (MQ=255)
                                                                                              aCGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑T‑‑‑T‑TCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATc       <  1:532806/100‑1 (MQ=255)
                                                                                               cGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑T‑‑‑T‑TCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCa      <  2:2751557/100‑1 (MQ=255)
                                                                                                 ccTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑T‑‑‑T‑TCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGt    <  2:2944052/100‑1 (MQ=255)
                                                                                                  cTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑T‑‑‑T‑TCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTc   <  2:1087708/100‑1 (MQ=255)
                                                                                                   ttATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑T‑‑‑T‑TCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCt  >  2:1407773/1‑100 (MQ=255)
                                                                                                   |                                                                                                          
TTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GAAT‑T‑‑‑T‑TCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCT  >  NC_000913/3705885‑3706083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑