Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9850TC41.9% 238.5 / 312.7 247intergenic (‑195/+113)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base T (76/67);  new base C (90/13);  total (166/81)
Fisher's exact test for biased strand distribution p-value = 8.48e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-01

Only 100 of 250 total aligned reads displayed.

GCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCT  >  NC_000913/3705887‑3706083
                                                                                                  |                                                                                                         
   gcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATcc                                                                                                       >  1:15593/1‑100 (MQ=255)
     cGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCgg                                                                                                     >  1:101277/1‑100 (MQ=255)
       gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                                   <  2:1002249/100‑1 (MQ=255)
        aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGcat                                                                                                  <  2:520025/100‑3 (MQ=37)
           gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACa                                                                                              >  2:2448296/1‑100 (MQ=255)
           gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGCATaaa                                                                                               <  1:1461271/100‑3 (MQ=25)
            cgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaa                                                                                             >  1:1930134/1‑100 (MQ=255)
            cgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGcataaac                                                                                              <  2:2290683/100‑7 (MQ=25)
             gcTGCACGCAGAGGCACAGCGCCACCGTGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaa                                                                                            >  2:532805/1‑100 (MQ=255)
               tGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAAt                                                                                          >  1:729796/1‑100 (MQ=255)
                 cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCg                                                                                        <  1:2767142/100‑1 (MQ=255)
                  aCGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGt                                                                                       >  1:1359082/1‑100 (MQ=255)
                   cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                                      >  1:1831186/1‑100 (MQ=255)
                   cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                                      >  1:2944069/1‑100 (MQ=255)
                    gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                                     <  2:2847338/100‑1 (MQ=255)
                      agagGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGcga                                                                                   >  2:2039708/1‑98 (MQ=255)
                      agagGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCaa                                                                                   <  1:356623/100‑1 (MQ=255)
                          gCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCCTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc                                                                               <  1:216976/100‑1 (MQ=255)
                           cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                              >  1:1768781/1‑100 (MQ=255)
                           cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                              >  1:1116225/1‑100 (MQ=255)
                           cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                              >  1:3032276/1‑100 (MQ=255)
                           cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGAGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                              >  2:1798117/1‑100 (MQ=255)
                            acaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa                                                                             >  2:2759536/1‑100 (MQ=255)
                             caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                                            >  2:380572/1‑100 (MQ=255)
                             caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                                            <  2:181666/100‑1 (MQ=255)
                             caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                                            <  2:1059568/100‑1 (MQ=255)
                               gcgcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATAc                                                                          >  2:1016575/1‑100 (MQ=255)
                                 gcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAt                                                                        >  2:312023/1‑100 (MQ=255)
                                 gcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAt                                                                        <  2:2105529/100‑1 (MQ=255)
                                  ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                                                                       >  2:3303309/1‑100 (MQ=255)
                                   cACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTg                                                                      >  2:1264582/1‑100 (MQ=255)
                                    aCCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGc                                                                     >  1:1597698/1‑100 (MQ=255)
                                      cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAg                                                                  <  1:2392299/100‑1 (MQ=255)
                                      cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAg                                                                  <  1:1747026/100‑1 (MQ=255)
                                      cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATGCAATACATTGCAg                                                                  >  2:1510990/1‑100 (MQ=255)
                                           ataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAtt‑‑‑t                                                        <  1:2149524/100‑1 (MQ=255)
                                             aCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAACTCGTGCAAATTCAATACCTTGCAGAATT‑‑‑TTc                                                      <  1:2256191/100‑1 (MQ=255)
                                                gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTCGTa                                                   >  1:1043269/1‑100 (MQ=255)
                                                 aTTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTCGTAg                                                  <  1:312024/100‑1 (MQ=255)
                                                    gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTCGTAGGcc                                               <  2:1650893/100‑1 (MQ=255)
                                                    gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTCGTAGGcc                                               <  2:2773431/100‑1 (MQ=255)
                                                       gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTCGTAGGCCTGa                                            >  1:498859/1‑100 (MQ=255)
                                                       gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTCGTAGGCCTGa                                            >  1:58313/1‑100 (MQ=255)
                                                                  cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTCGTAGGCCTGATAAGCGGAgcg                                 <  1:1544037/100‑1 (MQ=255)
                                                                    ttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCa                               <  1:2039716/100‑1 (MQ=255)
                                                                     tttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAATCATGCAAATCCAAAATATTGCAGGAGT‑‑‑TATGTAGGCCTGATAAGCGTAGCGCATc                             >  1:669997/8‑100 (MQ=18)
                                                                      gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATc                             >  2:1326802/1‑100 (MQ=255)
                                                                      gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATc                             >  2:1169837/1‑100 (MQ=255)
                                                                      gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATc                             <  2:349710/100‑1 (MQ=255)
                                                                       tgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAAC‑‑‑CTTGTAGGCCTGATAAGCGTAGCGCATCa                            >  2:2585220/6‑100 (MQ=17)
                                                                       cgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACGAAATCGTGCTAATTCAATATATTGTGGGCTC‑‑‑CACGTAGGCCTGATAAGCGTAGCGCATCa                            >  1:2728472/6‑100 (MQ=21)
                                                                        gtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCC‑‑‑CTTGTAGGCCTGATAAGCGTAGCGCATCAgg                          >  2:738645/5‑100 (MQ=18)
                                                                        gtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTT‑‑‑CACGTAGGCCTGATAAGCGTAGCGCATCAg                           >  1:841995/5‑100 (MQ=17)
                                                                        gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAAAGCATGAAAATTCAATATATTGCAGGAGC‑‑‑TGCGTAGGCCTGATAAGCGTAGCGCATCAg                           >  1:2292466/5‑100 (MQ=18)
                                                                         taggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAAGTCGTATAAATTCAATGTATTGCAGTTTA‑‑‑TTGGTAGGCCTGATAAGCGCAGCGCATCAgg                          >  1:3230938/4‑100 (MQ=21)
                                                                          aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAgg                          >  1:2498707/3‑100 (MQ=21)
                                                                          aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATAT‑‑‑GACGTAGGCCTGATAAGCGTAGCGCATCAGGcg                        >  1:1298502/3‑99 (MQ=21)
                                                                          aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTA‑‑‑TTGGTAGGCCTGATAAGCGCAGCGCATCAGGc                         >  1:883459/3‑100 (MQ=21)
                                                                          aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATC‑‑‑CTCGTAGGCCTGATAAGCGTAGCGCATCAGGc                         >  2:1567046/3‑100 (MQ=21)
                                                                          aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGC‑‑‑TGCGTAGGCCTGATAAGCGTAGCGCATCAGGc                         >  2:1592886/3‑100 (MQ=18)
                                                                           tGCCGGATGCTGCGTTAACGCCTTATCCGGCCTACAAAATCGGGCAAAGTCAATACATTGCAGAATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCa                        >  2:2675484/1‑100 (MQ=255)
                                                                           ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAAC‑‑‑CTTGTAGGCCTGATAAGCGTAGCGCATCAGGCa                        >  2:1890783/2‑100 (MQ=17)
                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATAT‑‑‑GACGTAGGCCTGATAAGCGTAGCGCATCAGGcga                       >  2:643025/2‑98 (MQ=21)
                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAA‑‑‑TTC‑‑AGGCCTGATAAGCGTAGCGCATCAGGctt                       >  2:1444908/2‑98 (MQ=21)
                                                                           ggCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCa                        >  2:596407/2‑100 (MQ=17)
                                                                            gctggataaggcgTTCACGCCGCATCCAGCCTACAAAATTGTGCAAAATCAATGGATTGCACAGCT‑‑‑AGCGTAGGCCTGATAAGCGTAGCGCATCAGGCag                       >  1:129130/10‑99 (MQ=25)
                                                                            gctggataaggcgTTCACGCCGCATCCAGCCTACAAAATTGTGCAAAATCAATGGATTGCACAGCT‑‑‑AGCGTAGGCCTGATAAGCGTAGCGCATCAGGCag                       >  2:1240779/10‑99 (MQ=25)
                                                                            gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAAC‑‑‑CTTGTAGGCCTGATAAGCGTAGCGCATCAGGCag                       >  1:2568400/1‑99 (MQ=18)
                                                                            gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGACC‑‑‑ATGTAGGCCTGATAAGCGTAGCGCATCAGGCag                       >  2:3344299/1‑99 (MQ=18)
                                                                            gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACGAAATCGTGCTAATTCAATATATTGTGGGCTC‑‑‑CACGTAGGCCTGATAAGCGTAGCGCATCAGGCag                       >  1:1417800/1‑99 (MQ=18)
                                                                             ccggataaggcgTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAAC‑‑‑CTTGTAGGCCTGATAAGCGTAGCGCATCAGGCagt                      >  2:159578/9‑98 (MQ=18)
                                                                             ccggataaggcgTTCACGCCGCATCCTGCCTACAAAATTGTGTAAATTCAATAAATTGATCGATC‑‑‑CACGTAGGCCTGATAAGCGTAGCGCATCAGGCagt                      >  1:2231219/9‑98 (MQ=21)
                                                                             ccggataaggcgTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGACC‑‑‑ATGTAGGCCTGATAAGCGTAGCGCATCAGGCagt                      >  2:1717498/9‑98 (MQ=18)
                                                                             ccGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGC‑‑‑TGCGTAGGCCTGATAAGCGTAGCGCATCAGGCagt                      >  2:310193/1‑98 (MQ=17)
                                                                               ggataaggcgTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAAC‑‑‑CTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                    >  2:2591136/7‑100 (MQ=18)
                                                                               ggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCC‑‑‑ATGTAGGCCTGATAAGCGTAGCGCATCAGGCAattt                    >  2:2383002/7‑100 (MQ=18)
                                                                               ggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTT‑‑‑CACGTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                    >  2:3040246/7‑100 (MQ=17)
                                                                               ggataaggcgTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCATGAGC‑‑‑CACGTAGGCCTGATAAGCGTAACGCATCAGGCAgtt                     >  1:1861010/7‑99 (MQ=17)
                                                                               ggataaggcgTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTA‑‑‑TTGGTAGGCCTGATAAGCGCAGCGCATCAGGCaatct                    >  2:1628485/7‑96 (MQ=25)
                                                                               ggataaggcgTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTA‑‑‑TTGGTAGGCCTGATAAGCGCAGCGCATCAGGCaatct                    >  2:2172710/7‑96 (MQ=21)
                                                                               ggataaggcgTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGATCGATC‑‑‑CACGTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                    >  1:411605/7‑100 (MQ=21)
                                                                               ggataaggcgTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGAC‑‑‑TATGTAGGCCTGATAAGCATAGCGCATCAGGCagctt                    >  1:749690/7‑96 (MQ=18)
                                                                               ggataaggcgTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGAT‑‑‑TATGTAGGCCTGATAAGCGTAGCGCATCAGGCAattt                    >  1:2861830/7‑100 (MQ=17)
                                                                               ggataaggcgTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGAT‑‑‑TATGTAGGCCTGATAAGCGTAGCGCATCAGGCAattt                    >  1:1205073/7‑100 (MQ=17)
                                                                               ggATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCAtttt                    >  1:1630770/1‑100 (MQ=255)
                                                                                gataaggcgTTCACGCCGCATCCGGCCTACAAAAGCACGCAAACTCAATATATTGCAGAGAT‑‑‑CATGTAGGCCTGATAAGCGTAGCGCATCAGGCAattta                   >  2:975239/6‑99 (MQ=17)
                                                                                gataaggcgTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAGT‑‑‑TATGTAGGCCTGATAAGCGTAGCGCATCAGGCAtttat                   >  1:1215784/6‑97 (MQ=21)
                                                                                   gcggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgc                 >  1:1156189/1‑99 (MQ=255)
                                                                                    cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct               >  2:2550205/1‑100 (MQ=255)
                                                                                    aggcgTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATT‑‑‑ATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATtttgcgt               >  1:201529/2‑98 (MQ=17)
                                                                                        gTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgn           <  1:3070430/100‑2 (MQ=255)
                                                                                        gTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgc           >  1:1241984/1‑100 (MQ=255)
                                                                                         ttAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgcc          <  2:833073/100‑1 (MQ=255)
                                                                                          tAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCa         >  1:3318455/1‑100 (MQ=255)
                                                                                            aCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATc       <  1:532806/100‑1 (MQ=255)
                                                                                               ccTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGt    <  2:2944052/100‑1 (MQ=255)
                                                                                                cTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTc   <  1:380573/100‑1 (MQ=255)
                                                                                                cTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTc   <  2:1087708/100‑1 (MQ=255)
                                                                                                 ttATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTCGTAGGCGTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCt  <  1:1938700/100‑1 (MQ=255)
                                                                                                 ttATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCt  >  2:1407773/1‑100 (MQ=255)
                                                                                                  |                                                                                                         
GCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCT  >  NC_000913/3705887‑3706083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: