Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,520,5041.T21.1% 485.4 / 170.2 177intergenic (‑180/+167)yjhU/yjhFputative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
Reads supporting (aligned to +/- strand):  ref base . (63/75);  new base T (19/18);  total (82/95)
Fisher's exact test for biased strand distribution p-value = 5.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 180 total aligned reads displayed.

ATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GATCGCCAGCGATTCGTCACCGTGCA  >  NC_000913/4520405‑4520600
                                                                                                    |                                                                                                 
 tGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAACATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAACCGGAGACATG‑c                                                                                                  >  2:3309022/1‑100 (MQ=255)
   aTTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTc                                                                                                <  2:508845/100‑1 (MQ=255)
     tttATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCg                                                                                              <  2:64836/100‑1 (MQ=255)
     tttATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCg                                                                                              <  1:3137255/100‑1 (MQ=255)
        ataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCt                                                                                           <  2:1275798/100‑1 (MQ=38)
        ataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCt                                                                                           <  1:2023244/100‑1 (MQ=38)
        ataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCt                                                                                           <  2:3127197/100‑1 (MQ=38)
         taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCtt                                                                                          <  2:362016/100‑1 (MQ=38)
          aCAGATATTTTATTCTTGTACATGAAATAAACCCGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCtt                                                                                          <  2:1097120/99‑1 (MQ=37)
           cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTa                                                                                        >  2:2495042/1‑100 (MQ=255)
           cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTa                                                                                        >  1:2174864/1‑100 (MQ=255)
            aGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTAc                                                                                       <  1:243985/100‑1 (MQ=255)
            aGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCTTTAc                                                                                       <  1:452430/100‑1 (MQ=38)
            aGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCTTTAc                                                                                       <  1:3082373/100‑1 (MQ=38)
            aGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCTTTAc                                                                                       <  1:2099228/100‑1 (MQ=38)
             gATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACa                                                                                      <  2:1003237/100‑1 (MQ=255)
              atatTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAg                                                                                     <  1:2881609/100‑1 (MQ=255)
              atatTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAg                                                                                     <  2:2651399/100‑1 (MQ=255)
                atTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTa                                                                                   >  1:2823694/1‑100 (MQ=255)
                 ttttATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTAt                                                                                  >  2:588112/1‑100 (MQ=255)
                 ttttATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTAt                                                                                  <  2:3016114/100‑1 (MQ=37)
                    tATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCTCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTAtggg                                                                               <  2:136260/100‑4 (MQ=25)
                      ttCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTg                                                                             >  1:2993754/1‑100 (MQ=255)
                      ttCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTAtggggg                                                                             <  2:1433534/100‑6 (MQ=25)
                      ttCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTAtggggg                                                                             <  2:2297854/100‑6 (MQ=25)
                       tCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTg                                                                             <  2:2895499/99‑1 (MQ=255)
                        cTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTgag                                                                           <  2:263944/100‑1 (MQ=255)
                          tttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGaa                                                                         >  1:892782/1‑100 (MQ=255)
                             aCATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACCGTATTACTGAGAATac                                                                      >  2:1962739/1‑100 (MQ=255)
                              cATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATaca                                                                     >  2:2511487/1‑100 (MQ=255)
                              cATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATaca                                                                     >  1:1257926/1‑100 (MQ=255)
                               aTGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATacac                                                                    <  1:1841862/100‑1 (MQ=255)
                                tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                   >  1:3283728/1‑100 (MQ=255)
                                tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                   >  2:1550263/1‑100 (MQ=255)
                                tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                   <  2:1809758/100‑1 (MQ=255)
                                tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                   <  1:2649451/100‑1 (MQ=255)
                                  taaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCa                                                                 <  1:2501427/99‑1 (MQ=255)
                                  aaaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCa                                                                 <  1:1148029/100‑1 (MQ=255)
                                   aaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCaa                                                                >  2:2501415/1‑99 (MQ=255)
                                     tAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGTCTTTACAGTATTACTGATAATACACCCAtga                                                              <  1:2256381/100‑3 (MQ=255)
                                     tAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCa                                                              <  1:626808/100‑1 (MQ=255)
                                        cctggaatgtcATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGc                                                           >  1:1077693/11‑100 (MQ=255)
                                         ccAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCa                                                          >  2:1720145/1‑100 (MQ=255)
                                            gCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATa                                                        <  2:934345/99‑1 (MQ=255)
                                             cATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAAt                                                      >  1:2518632/1‑100 (MQ=255)
                                             cATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAAt                                                      <  1:2584095/100‑1 (MQ=255)
                                              aTACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATc                                                     >  2:318632/1‑100 (MQ=255)
                                              aTACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATc                                                     <  2:2779275/100‑1 (MQ=255)
                                                 ccATTTGAGCGATCGCCCGCTGTCCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGaa                                                  >  2:2666274/1‑100 (MQ=255)
                                                   aTTTGAGCGCTCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAgg                                                <  2:3162965/100‑1 (MQ=255)
                                                    tttGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGt                                               <  1:248009/100‑1 (MQ=255)
                                                     ttGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGtt                                              >  2:3223823/1‑100 (MQ=255)
                                                     ttGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGtt                                              >  2:1534290/1‑100 (MQ=255)
                                                      tGAGCGATCGCCCGCTGTTGCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTc                                             <  2:1673510/100‑1 (MQ=255)
                                                      tGAGCGATCGCCCCCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACAACCATCAAGCATAATCGAAGGTTc                                             <  1:1950442/100‑1 (MQ=255)
                                                       gAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCa                                            <  1:1164190/100‑1 (MQ=255)
                                                         gCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAat                                          >  2:862216/1‑100 (MQ=255)
                                                         gCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAat                                          <  2:1881793/100‑1 (MQ=255)
                                                         gCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAat                                          >  1:1881800/1‑100 (MQ=255)
                                                         gCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAat                                          >  1:1413827/1‑100 (MQ=255)
                                                            aTCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGAAATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATc                                       >  1:2369515/1‑100 (MQ=255)
                                                               gCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAatat                                        >  2:134348/1‑96 (MQ=255)
                                                               gCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGcc                                    >  1:2786133/1‑100 (MQ=255)
                                                                    cTGTTCCCCCATCCTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCttt                               >  1:2207555/1‑100 (MQ=255)
                                                                      tctcagtaaTACTGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCttttt                             >  1:1004951/9‑100 (MQ=21)
                                                                      tctcagtaaTACTGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCttttt                             >  1:1004921/9‑100 (MQ=21)
                                                                      gTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCttttt                             >  1:1640449/1‑100 (MQ=255)
                                                                      gTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCttttt                             <  2:2823681/100‑1 (MQ=255)
                                                                        tCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑Ga                          <  1:1669832/100‑1 (MQ=255)
                                                                        tCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑Ga                          <  1:1669956/100‑1 (MQ=255)
                                                                         cccccATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GAt                         >  2:2890620/1‑100 (MQ=255)
                                                                           cccATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GATCg                       <  2:1248866/100‑1 (MQ=255)
                                                                           cccATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GATCg                       <  1:1926164/100‑1 (MQ=255)
                                                                             cATATTGTAAAGCCTGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GATct                       <  2:800067/98‑2 (MQ=255)
                                                                                aCTGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GATCGCCAGc                  >  2:22243/1‑100 (MQ=38)
                                                                                 cTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GATCGCCAGCg                 >  2:971826/1‑100 (MQ=255)
                                                                                 cTGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GATCGCCAGCg                 >  1:133900/1‑100 (MQ=38)
                                                                                  tGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GATCGCCAGCGa                >  1:357961/1‑100 (MQ=255)
                                                                                  tGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GATCGCCAGCGa                >  1:2636225/1‑100 (MQ=38)
                                                                                   gTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTAGATCggaagagc                >  1:2435230/1‑93 (MQ=255)
                                                                                   gTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GATCGCCAGCGAt               <  2:3137256/100‑1 (MQ=255)
                                                                                   gTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCCTTTT‑GATCGCCAGCGAt               >  1:3236669/1‑100 (MQ=38)
                                                                                     aaaGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GATCGCCAGCGATTc             <  2:2378199/100‑1 (MQ=255)
                                                                                     aaaGCCGGAG‑CATGTCTCCGGCTTTACAGTTTTTCTTAGAATACACCCATCAAGCATAATCGAAGGTTTAATATCGCCCCTTTTT‑TATCGCCCGCGAt               >  2:80568/1‑98 (MQ=25)
                                                                                     aaaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GATCGCCAGCGATTc             >  2:2070313/1‑100 (MQ=38)
                                                                                     aaaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GATCGCCAGCGATTc             >  1:215202/1‑100 (MQ=38)
                                                                                     aaaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GATCGCCAGCGATTc             >  1:27192/1‑100 (MQ=38)
                                                                                     aaaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GATCGCCAGCGATTc             >  1:564413/1‑100 (MQ=38)
                                                                                     aaaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GATCGCCAGCGATTc             >  2:1888752/1‑100 (MQ=38)
                                                                                       aGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GATCGCCAGCGATTCGt           >  2:3065161/1‑100 (MQ=255)
                                                                                        gCCGGAGGCATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GATCGCCAGCGATTCGTc          >  1:1762622/1‑100 (MQ=255)
                                                                                        gCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GATCGCCAGcttagtttt          >  1:711136/1‑92 (MQ=255)
                                                                                        gCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GATCGCCAGCGATTCGTc          >  1:272060/1‑100 (MQ=255)
                                                                                        gCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GATCGCCAGCGATTCGTc          <  1:2583069/100‑1 (MQ=255)
                                                                                          tccgatcttG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GATCGCCAGCGATTCGTCAc        <  1:1462176/92‑1 (MQ=255)
                                                                                              gaCATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GATCGCCAGCGATTCGTCACCGTg    <  2:357960/100‑1 (MQ=255)
                                                                                               aCATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GATCGCCAGCGATTCGTCACCGTg    <  2:1257922/99‑1 (MQ=255)
                                                                                               aCATG‑CTCCGGCTGTAGAGTATTACTGAGAATACACCCAGCAAGCATAATCGAAGGGTCAATGGCGCCCCTTTTT‑GATCGCCAGCGATTCGTCACCGTGc   <  2:1301096/100‑1 (MQ=255)
                                                                                                cATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GATCGCCAGCGATTCGTCACCGTGCa  >  1:969457/1‑100 (MQ=255)
                                                                                                cATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GATCGCCAGCGATTCGTCACCGTGCa  <  1:1752831/100‑1 (MQ=255)
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ATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTT‑GATCGCCAGCGATTCGTCACCGTGCA  >  NC_000913/4520405‑4520600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑