Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,864,7630AT20.6% 275.0 / 70.6 126intergenic (+29/+19)yeaD/yeaED‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base A (49/51);  new base T (15/11);  total (64/62)
Fisher's exact test for biased strand distribution p-value = 5.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.41e-01

Only 100 of 130 total aligned reads displayed.

CAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTT  >  NC_000913/1864664‑1864861
                                                                                                   |                                                                                                  
cAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCaa                                                                                                    <  1:1219769/100‑1 (MQ=255)
 aGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAAt                                                                                                   <  1:2320937/100‑1 (MQ=255)
 aGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAAt                                                                                                   <  1:2026909/100‑1 (MQ=255)
 aGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAAt                                                                                                   <  1:762926/100‑1 (MQ=255)
  gAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATg                                                                                                  >  1:2547112/1‑100 (MQ=255)
  gAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATg                                                                                                  <  1:777915/100‑1 (MQ=255)
  gAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAACCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCGGGGTGCAATg                                                                                                  >  2:2203943/1‑100 (MQ=255)
   acaCGCAAAAAGTGCCCAAAGCGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATg                                                                                                  <  1:2675215/97‑1 (MQ=255)
      cGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATc                                                                                              >  2:101926/1‑100 (MQ=255)
      cGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCacc                                                                                              <  1:1879996/100‑3 (MQ=255)
      cGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCacc                                                                                              <  1:1878457/100‑3 (MQ=255)
      cGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAAGCCATTCGCGTTGCGAAACGTTAATTGACGTTAATGTTGTGTGCCGGGTGCAATGCAt                                                                                               <  2:675655/99‑1 (MQ=255)
          aaaaGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGc                                                                                          <  1:1889077/100‑1 (MQ=255)
          aaaaGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGc                                                                                          <  2:3301399/100‑1 (MQ=38)
            aaGTGACCAAAGAGAAACCTGTACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGcac                                                                                        <  1:3069142/100‑1 (MQ=38)
            aaGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcac                                                                                        <  1:3140311/100‑1 (MQ=255)
            aaGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCACGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGcac                                                                                        <  2:2358327/100‑1 (MQ=38)
             aGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcaca                                                                                       <  1:2580951/100‑1 (MQ=255)
              gTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcacac                                                                                      <  2:9286/100‑1 (MQ=255)
              gTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGCGTGCCGGATGCATTGCACCCGGcacac                                                                                      <  2:1657186/100‑1 (MQ=38)
                 aCCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACacaac                                                                                   <  2:804135/100‑1 (MQ=255)
                  ccAAAGCGAAACCTGCCCATCTGGCGCAATCCATTCGCGTTGCGACACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGCACacaaca                                                                                  <  1:1831629/100‑1 (MQ=25)
                      agagAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATcac                                                                              <  1:2285889/100‑1 (MQ=255)
                        agaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCacac                                                                            <  2:1961062/100‑1 (MQ=255)
                         gaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACAcc                                                                           >  2:1230072/1‑100 (MQ=255)
                         gaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACAcc                                                                           >  2:1230978/1‑100 (MQ=255)
                         gaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACAcc                                                                           >  2:2848666/1‑100 (MQ=255)
                         gaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGAGTGCCGGGTGCAATGCATCCGGCACACAACATCACAcc                                                                           >  2:1827061/1‑100 (MQ=255)
                          aaaCCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCa                                                                          <  1:58293/100‑1 (MQ=255)
                           aaCCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCat                                                                         >  2:2118462/1‑100 (MQ=255)
                             ccTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCatat                                                                       >  1:593662/1‑100 (MQ=255)
                             ccTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCatat                                                                       >  1:1653752/1‑100 (MQ=255)
                              cTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCATACCATATc                                                                      >  1:1685532/1‑100 (MQ=255)
                                   caTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAgcg                                                                 <  1:160822/100‑1 (MQ=255)
                                     tCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCa                                                               >  2:641312/1‑100 (MQ=255)
                                      cTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAg                                                              <  1:2877992/100‑1 (MQ=255)
                                      cTGGCGCAAGCCATTCGCGTTGCGAGACGTTAATTTACGTGAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAgcgc                                                                <  2:263122/98‑1 (MQ=255)
                                        ggCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGtt                                                            >  1:2377426/1‑100 (MQ=255)
                                          cgcAATCCATTCGCGTTGCGAAACGTTAATTTCCGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGtttt                                                          <  2:440902/100‑1 (MQ=255)
                                            cAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGttttt                                                         <  2:1653748/99‑1 (MQ=255)
                                            cAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTc                                                        >  2:3102056/1‑100 (MQ=255)
                                               tCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtt                                                     <  2:68411/100‑1 (MQ=255)
                                                 cATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtttt                                                   <  1:1969415/100‑1 (MQ=255)
                                                 cATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtttt                                                   <  1:2641544/100‑1 (MQ=255)
                                                 cATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtttt                                                   >  2:2402220/1‑100 (MQ=255)
                                                   ttCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTgg                                                 <  2:1263163/100‑1 (MQ=255)
                                                       cgTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTTGttc                                              >  2:433418/1‑97 (MQ=255)
                                                        gTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCg                                            <  1:485996/100‑1 (MQ=255)
                                                          tGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGa                                          >  2:1594211/1‑100 (MQ=255)
                                                          tGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGa                                          >  2:103408/1‑100 (MQ=255)
                                                           gCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGat                                         >  1:530472/1‑100 (MQ=255)
                                                           gCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGat                                         >  2:753150/1‑100 (MQ=255)
                                                           gCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGat                                         <  2:3338911/100‑1 (MQ=255)
                                                            cGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGata                                        >  1:208782/1‑100 (MQ=255)
                                                                aCGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGcc                                    <  2:551277/100‑1 (MQ=255)
                                                                aCGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGcc                                    <  2:2791877/100‑1 (MQ=255)
                                                                 cGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCt                                   <  1:1992365/100‑1 (MQ=255)
                                                                 cGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTCGGTGCCGGATATGCCt                                   >  2:1962851/1‑100 (MQ=255)
                                                                 cGTTAATTTACGTTAATGTTGTGTGCAGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCt                                   >  1:2042520/1‑100 (MQ=255)
                                                                   ttAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAATATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTa                                 <  2:633325/100‑1 (MQ=255)
                                                                   ttAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTa                                 <  1:1650297/100‑1 (MQ=255)
                                                                     aaTTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATc                               >  2:1120178/1‑100 (MQ=255)
                                                                     aaTTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATc                               >  1:494811/1‑100 (MQ=255)
                                                                       cttACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCTCACTACATCACACCATATCCAGCGCCGTTTTTCCTTTTCGTGCCGGATATGCCTTATCCa                             >  2:1243765/2‑100 (MQ=255)
                                                                        ttACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTTCCGGATATGCCTTATCCAg                            >  2:919047/1‑100 (MQ=255)
                                                                        tctcGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAg                            <  2:1929266/97‑1 (MQ=255)
                                                                         tACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGc                           <  2:2213878/100‑1 (MQ=255)
                                                                          aCGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCa                          <  1:1553469/100‑1 (MQ=255)
                                                                             ttAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAg                       >  1:1975170/1‑100 (MQ=255)
                                                                              tAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGc                      <  2:354857/100‑1 (MQ=255)
                                                                               aaTGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCt                     <  2:595875/100‑1 (MQ=255)
                                                                                  gttgtGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAAt                  <  2:208781/100‑1 (MQ=255)
                                                                                    tgtgtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTc                >  1:1049361/1‑100 (MQ=255)
                                                                                    tgtgtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTc                <  1:2874556/100‑1 (MQ=255)
                                                                                    tgtgtgCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTc                >  2:2160609/1‑100 (MQ=38)
                                                                                    tgtgtgCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTc                >  1:677447/1‑100 (MQ=38)
                                                                                     gtgtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTcc               >  2:703256/1‑100 (MQ=255)
                                                                                     gtgtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTcc               >  1:1578091/1‑100 (MQ=255)
                                                                                     gtgtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTcc               >  1:111156/1‑100 (MQ=255)
                                                                                      tgtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGTTGCCGGATATGCCTTATCCAGCATAGCTAATTcc               >  2:1847548/1‑99 (MQ=255)
                                                                                       gtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTTTCCAGCATAGCTAATTCCGc             <  2:2042512/100‑1 (MQ=255)
                                                                                         nccGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTg           >  1:2676854/2‑100 (MQ=255)
                                                                                         gCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTg           <  2:177422/100‑1 (MQ=255)
                                                                                         gCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTg           <  1:795372/100‑1 (MQ=255)
                                                                                         gCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTg           >  1:1228283/1‑100 (MQ=255)
                                                                                         gCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTg           <  1:1962859/100‑1 (MQ=255)
                                                                                         gCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTg           <  2:1228279/100‑1 (MQ=255)
                                                                                         gCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTg           >  2:196965/1‑100 (MQ=38)
                                                                                         gCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTg           >  2:3334890/1‑100 (MQ=38)
                                                                                         gCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTg           >  1:419818/1‑100 (MQ=38)
                                                                                         gCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTg           >  1:2133270/1‑100 (MQ=38)
                                                                                           cGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAACATAGCTAATTCCGCTgaa         >  2:348464/1‑100 (MQ=255)
                                                                                            gggTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaag        <  1:996374/100‑1 (MQ=255)
                                                                                            gggTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaag        <  2:458210/100‑1 (MQ=255)
                                                                                            gggTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaa         >  2:1490242/1‑99 (MQ=255)
                                                                                            ggatGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaag        <  1:3334908/97‑1 (MQ=255)
                                                                                               tGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGaaa     <  2:532687/100‑1 (MQ=255)
                                                                                               tGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGaaa     >  2:2664188/1‑100 (MQ=255)
                                                                                                gCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAg    >  1:2797514/1‑100 (MQ=255)
                                                                                                  aaTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGtt  >  1:2779376/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
CAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTT  >  NC_000913/1864664‑1864861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑