Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,143,641 G→A H243Y (CAT→TAT)  rne ← endoribonuclease; RNA‑binding protein;RNA degradosome binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,143,6410GA98.5% 439.8 / ‑3.8 129H243Y (CAT→TAT) rneendoribonuclease; RNA‑binding protein;RNA degradosome binding protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (58/69);  total (59/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 129 total aligned reads displayed.

TCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGC  >  NC_000913/1143545‑1143736
                                                                                                |                                                                                               
tCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTg                                                                                              <  1:254160/100‑1 (MQ=255)
tCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTg                                                                                              >  2:1364945/1‑100 (MQ=255)
 cTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGa                                                                                             >  2:1357556/1‑100 (MQ=255)
  tgtgACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGAc                                                                                            >  2:1178787/1‑100 (MQ=255)
  tgtgACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGAc                                                                                            >  1:1654926/1‑100 (MQ=255)
   gtgACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACg                                                                                           <  1:1877070/100‑1 (MQ=255)
   gtgACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACg                                                                                           <  2:3314654/100‑1 (MQ=255)
   gtgACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACg                                                                                           <  2:3345334/100‑1 (MQ=255)
     gACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTg                                                                                         >  1:3190272/1‑100 (MQ=255)
     gACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTg                                                                                         >  2:2911493/1‑100 (MQ=255)
     gACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTg                                                                                         >  1:1065618/1‑100 (MQ=255)
      aCTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGc                                                                                        >  1:1274475/1‑100 (MQ=255)
       cTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGcc                                                                                       <  2:2020655/100‑1 (MQ=255)
       cTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGcc                                                                                       <  1:1977402/100‑1 (MQ=255)
       cTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGcc                                                                                       <  2:2675389/100‑1 (MQ=255)
         cGATCTGGTACTGGCTGAACAGCGTGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAg                                                                                     <  2:2202290/100‑1 (MQ=255)
          gATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGt                                                                                    >  1:2598812/1‑100 (MQ=255)
          gATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGt                                                                                    >  1:519637/1‑100 (MQ=255)
             cTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCg                                                                                 >  1:1699428/1‑100 (MQ=255)
             cTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCg                                                                                 <  2:368982/100‑1 (MQ=255)
              tGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGCAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGa                                                                                >  2:319485/1‑100 (MQ=255)
              tGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGa                                                                                >  1:65952/1‑100 (MQ=255)
                gTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCg                                                                                 <  2:3154235/97‑1 (MQ=255)
                   gTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACt                                                                           >  1:749779/1‑100 (MQ=255)
                     ggCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACttt                                                                         >  2:2826488/1‑100 (MQ=255)
                      gCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTc                                                                        >  2:218614/1‑100 (MQ=255)
                         gaccAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCggg                                                                     <  1:2343504/97‑1 (MQ=255)
                         gAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCggg                                                                     <  2:519636/100‑1 (MQ=255)
                           aCAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTCCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGt                                                                    <  1:835291/99‑1 (MQ=255)
                            cAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGtt                                                                   <  2:1300175/99‑1 (MQ=255)
                              gCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTCATGCAGCGATATGCTGACGTGACAGTTCGAGCACTTTCGGGTTATc                                                                >  2:3128746/1‑100 (MQ=255)
                              gCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATc                                                                >  1:1839028/1‑100 (MQ=255)
                              gCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATc                                                                <  1:2190807/100‑1 (MQ=255)
                              gCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATc                                                                <  1:218615/100‑1 (MQ=255)
                               cGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCg                                                               <  2:3089135/100‑1 (MQ=255)
                                 ggATCTCTCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGAt                                                             >  1:1769971/1‑100 (MQ=255)
                                 ggATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGAt                                                             >  2:2217410/1‑100 (MQ=255)
                                 ggATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGAt                                                             <  1:855483/100‑1 (MQ=255)
                                 ggATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGAt                                                             >  1:479253/1‑100 (MQ=255)
                                      tcGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGa                                                        >  1:3076885/1‑100 (MQ=255)
                                      tcGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGa                                                        <  1:3285188/100‑1 (MQ=255)
                                       cGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGAt                                                       <  1:2312648/100‑1 (MQ=255)
                                       cGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGAt                                                       >  2:3063799/1‑100 (MQ=255)
                                           ggTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCg                                                   >  2:177102/1‑100 (MQ=255)
                                            gtgtACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGc                                                  >  1:3187406/1‑100 (MQ=255)
                                             tgtACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGcc                                                 <  1:465229/100‑1 (MQ=255)
                                             tgtACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGcc                                                 <  1:2621158/100‑1 (MQ=255)
                                             tgtACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGcc                                                 <  2:554835/100‑1 (MQ=255)
                                              gtACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCg                                                >  1:2623752/1‑100 (MQ=255)
                                              gtACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCg                                                <  1:2826501/100‑1 (MQ=255)
                                               tACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGa                                               <  2:1069819/100‑1 (MQ=255)
                                                aCAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGAt                                              <  1:1072581/100‑1 (MQ=255)
                                                 cAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATg                                             >  2:804342/1‑100 (MQ=255)
                                                 cAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATg                                             >  2:2496590/1‑100 (MQ=255)
                                                    tttGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTcc                                          <  1:2456495/100‑1 (MQ=255)
                                                    tttGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTcc                                          <  2:2723630/100‑1 (MQ=255)
                                                     tggATTTTGCTGCGGAAATCCGGGCGACCTAATGCAGCGATACACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCt                                         >  1:2271056/3‑100 (MQ=255)
                                                       gATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGa                                       <  1:2706411/100‑1 (MQ=255)
                                                        aTTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGAc                                      <  1:210422/100‑1 (MQ=255)
                                                        aTTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGAc                                      >  2:1520280/1‑100 (MQ=255)
                                                         ttttGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACg                                     >  1:158335/1‑100 (MQ=255)
                                                         ttttGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACg                                     <  1:765124/100‑1 (MQ=255)
                                                             gctgctGAAATCCGGGCGACCTTATGCAGCGTTATACTGACGTGCCATTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaag                                 <  2:2261593/100‑1 (MQ=255)
                                                             gctgctGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaag                                 <  2:1258223/100‑1 (MQ=255)
                                                             gctgctGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaag                                 <  2:1223853/100‑1 (MQ=255)
                                                             gctgctGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaag                                 >  2:1195125/1‑100 (MQ=255)
                                                             gctgctGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaag                                 <  2:1135801/100‑1 (MQ=255)
                                                              cttctgAAATCCGGGCTACCTAATGCAGCGATATGCTGACGTCCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCCGACgtaagt                                <  1:181725/97‑1 (MQ=255)
                                                              ctgctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaagt                                <  1:1980871/100‑1 (MQ=255)
                                                              ctgctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaagt                                >  1:2539591/1‑100 (MQ=255)
                                                               tgctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaagta                               >  2:240454/1‑100 (MQ=255)
                                                                gctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaagtaa                              >  1:1845447/1‑100 (MQ=255)
                                                                    aaaTCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATcgc                          >  2:695117/1‑100 (MQ=255)
                                                                      aTCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCgg                        <  2:1039802/100‑1 (MQ=255)
                                                                        ccGGGCGACCTAATGCAGCGATATACTGACGTGCTAGTTCGCGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGaa                      >  2:451420/1‑100 (MQ=255)
                                                                          gggCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATg                    <  1:2389249/100‑1 (MQ=255)
                                                                             cGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATgcgc                 <  2:2271045/100‑1 (MQ=255)
                                                                             cGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATgcgc                 <  1:1979544/100‑1 (MQ=255)
                                                                             cGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATgcgc                 <  2:1818997/100‑1 (MQ=255)
                                                                             cGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATgcg                  <  2:786377/99‑1 (MQ=255)
                                                                              gACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAAGCGCGGAATGcgcg                >  1:1954586/1‑100 (MQ=255)
                                                                                ccTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGaa              <  2:2006360/100‑1 (MQ=255)
                                                                                 cTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAAc             <  2:1660928/100‑1 (MQ=255)
                                                                                  tAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACg            >  1:1169781/1‑100 (MQ=255)
                                                                                     tGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGAtt         <  2:1884940/100‑1 (MQ=255)
                                                                                     tGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGAtt         >  2:1474599/1‑100 (MQ=255)
                                                                                     tGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGAtt         >  1:3217412/1‑100 (MQ=255)
                                                                                     ngCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGAtt         >  2:1022983/2‑100 (MQ=255)
                                                                                      gCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTa        <  2:2837743/100‑1 (MQ=255)
                                                                                      gCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTa        <  2:452710/100‑1 (MQ=255)
                                                                                       cAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTAc       >  2:1325674/1‑100 (MQ=255)
                                                                                       cAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTAc       >  2:3353808/1‑100 (MQ=255)
                                                                                       cAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTAc       >  2:620392/1‑100 (MQ=255)
                                                                                        aGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGAtt         >  2:1245019/1‑97 (MQ=255)
                                                                                         gCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACTATTACGt     <  1:1770245/100‑1 (MQ=255)
                                                                                         gCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGt     >  1:2816290/1‑100 (MQ=255)
                                                                                           gATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTg   <  1:1636372/100‑1 (MQ=255)
                                                                                            atatACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGc  <  2:2955542/100‑1 (MQ=255)
                                                                                            atatACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGc  <  2:2830952/100‑1 (MQ=255)
                                                                                            atatACTGACGTGCCAGTTCCAGCACTTTCGTGTTATCCATAAGGATTTCGCCGATGTCCTGACGTATGTTATCGCGGAATGCGCGAAGGATTACg      <  2:753208/96‑1 (MQ=255)
                                                                                                |                                                                                               
TCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGC  >  NC_000913/1143545‑1143736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: