BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,146,997	G→A	T68T (ACG→ACA)	yceD →	DUF177 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,146,997	0	G	A	98.7%	515.2 / ‑2.7	151	T68T (ACG→ACA)	yceD	DUF177 family protein
Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (79/69); total (81/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01

Only 100 of 151 total aligned reads displayed.

AATCCGTAGTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAA  >  NC_000913/1146900‑1147093

  tCCGTAGTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTaa                                                                                              <  2:2548592/100‑1 (MQ=255)

  tCCGTAGTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTaa                                                                                              <  1:2660595/100‑1 (MQ=255)

    cGTAGTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACg                                                                                            >  1:440957/1‑100 (MQ=255)

      tAGTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCt                                                                                          <  1:1089006/100‑1 (MQ=255)

       aGTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTc                                                                                         >  1:3329754/1‑100 (MQ=255)

        gTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCg                                                                                        >  2:803821/1‑100 (MQ=255)

         tCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGa                                                                                       <  2:2773255/100‑1 (MQ=255)

          cAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAg                                                                                      <  1:1375161/100‑1 (MQ=255)

          cAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAg                                                                                      <  1:2829284/100‑1 (MQ=255)

            ntgtgGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAgtg                                                                                    >  2:2554542/2‑100 (MQ=255)

            gtgtGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAgtg                                                                                    >  1:587905/1‑100 (MQ=255)

              gtgGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTc                                                                                  >  1:3315784/1‑100 (MQ=255)

                ggACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAg                                                                                >  2:1824917/1‑100 (MQ=255)

                ggACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAg                                                                                >  1:1162886/1‑100 (MQ=255)

                 gACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGc                                                                               >  1:1590201/1‑100 (MQ=255)

                     gTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTg                                                                           >  2:2155613/1‑100 (MQ=255)

                       gATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCg                                                                         >  2:1673893/1‑100 (MQ=255)

                       gATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCg                                                                         >  2:2492631/1‑100 (MQ=255)

                        aTGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCgg                                                                        <  1:3066250/100‑1 (MQ=255)

                        aTGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCgg                                                                        <  2:2257492/100‑1 (MQ=255)

                         tctgGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCggg                                                                       <  2:2040143/98‑1 (MQ=255)

                          gtgGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGa                                                                      >  1:2994548/1‑100 (MQ=255)

                          gtgGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGa                                                                      >  1:375324/1‑100 (MQ=255)

                          gtgGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGa                                                                      >  1:1315663/1‑100 (MQ=255)

                          ggggAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGa                                                                      <  2:458492/98‑1 (MQ=255)

                            ggAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAg                                                                    >  1:1573780/1‑100 (MQ=255)

                            ggAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAg                                                                    >  1:1992437/1‑100 (MQ=255)

                               aTGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCg                                                                 <  2:201221/100‑1 (MQ=255)

                               aTGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCg                                                                 <  2:2748913/100‑1 (MQ=255)

                               aTGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCg                                                                 <  2:2749359/100‑1 (MQ=255)

                                tGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGt                                                                <  2:1162883/100‑1 (MQ=255)

                                tGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGt                                                                <  1:606791/100‑1 (MQ=255)

                                 ncTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGtt                                                               >  2:459809/2‑100 (MQ=255)

                                 gCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGtt                                                               <  1:2207631/100‑1 (MQ=255)

                                 gCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGtt                                                               >  2:430516/1‑100 (MQ=255)

                                 gCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGtt                                                               <  1:527375/100‑1 (MQ=255)

                                  cTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGttt                                                              >  1:2429165/1‑100 (MQ=255)

                                  cTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGttt                                                              >  2:1608262/1‑100 (MQ=255)

                                  cTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGttt                                                              <  1:2101545/100‑1 (MQ=255)

                                    ccATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTAc                                                            <  1:1878752/100‑1 (MQ=255)

                                        gTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACtcat                                                        >  2:1215618/1‑100 (MQ=255)

                                        gTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACtcat                                                        >  1:2696995/1‑100 (MQ=255)

                                        gTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACtcat                                                        >  2:2873139/1‑100 (MQ=255)

                                          cGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACtcatca                                                      >  2:908864/1‑100 (MQ=255)

                                            ttCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAgg                                                    <  2:1145346/100‑1 (MQ=255)

                                             tCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAgg                                                    >  1:943361/1‑99 (MQ=255)

                                               ncTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCt                                                 >  2:3094990/2‑100 (MQ=255)

                                               gCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCt                                                 <  1:812806/100‑1 (MQ=255)

                                               gCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGCGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCt                                                 >  1:1259880/1‑100 (MQ=255)

                                                cTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTa                                                <  2:375323/100‑1 (MQ=255)

                                                  aTCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTaca                                              >  1:43213/1‑100 (MQ=255)

                                                     gATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACaa                                           >  1:507325/1‑100 (MQ=255)

                                                     gATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACaa                                           >  2:3314790/1‑100 (MQ=255)

                                                     gATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACaa                                           >  2:1632752/1‑100 (MQ=255)

                                                      aTAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAATCCGTTTACTCATCAGGTCTACACaan                                          <  2:2949729/100‑2 (MQ=255)

                                                      aTAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAAc                                          <  2:541824/100‑1 (MQ=255)

                                                       tAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACg                                         <  2:1572751/100‑1 (MQ=255)

                                                       tAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACg                                         <  2:587904/100‑1 (MQ=255)

                                                       tAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACg                                         >  1:3096087/1‑100 (MQ=255)

                                                        aaCCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGt                                        >  1:3341309/1‑100 (MQ=255)

                                                          ccAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTAt                                      >  2:1227873/1‑100 (MQ=255)

                                                          ccAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTan                                      <  2:246302/100‑2 (MQ=255)

                                                          ccAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTAt                                      <  2:1485447/100‑1 (MQ=255)

                                                          ccAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTAt                                      >  1:3055092/1‑100 (MQ=255)

                                                          ccAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTAt                                      <  2:2288981/100‑1 (MQ=255)

                                                           cAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTAtt                                     <  1:719187/100‑1 (MQ=255)

                                                            aaCGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTg                                    >  1:3208674/1‑100 (MQ=255)

                                                                tctcGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGtttt                                <  1:1130216/100‑1 (MQ=255)

                                                                tctcGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGtttt                                <  1:2554753/100‑1 (MQ=255)

                                                                tctcGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGtttt                                <  1:2401905/100‑1 (MQ=255)

                                                                 ctcGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTa                               <  2:3014133/100‑1 (MQ=255)

                                                                 ctcGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTa                               <  1:194150/100‑1 (MQ=255)

                                                                  tcGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAg                              <  1:1227877/100‑1 (MQ=255)

                                                                  tcGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAg                              <  1:186996/100‑1 (MQ=255)

                                                                   ngCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGt                             >  1:305006/2‑100 (MQ=255)

                                                                    gCAGTGTTAAACGGTGATGCGAAGGTGACAGTAACGCTCGGGTGTCAGCGTTGCGGGAAGTCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAg                              <  2:2817314/98‑1 (MQ=255)

                                                                       gtgtTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCtg                         <  2:3329736/100‑1 (MQ=255)

                                                                       gtgtTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCt                          <  2:2793036/99‑1 (MQ=255)

                                                                        tgtTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTTTCAGCGTTGCGGGAAGCCGTTTACTCATCATGTCTACACAACGTATTGTTTTAGTCCtgt                        <  2:2362206/100‑1 (MQ=255)

                                                                         gtTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCtgtg                       >  2:1068696/1‑100 (MQ=255)

                                                                           tAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCg                     >  1:2969850/1‑100 (MQ=255)

                                                                              aCGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTc                  <  1:1493459/100‑1 (MQ=255)

                                                                               cGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTc                  >  2:546949/1‑99 (MQ=255)

                                                                               cGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCa                 >  2:2399603/1‑100 (MQ=255)

                                                                               atcgatagCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCa                 <  2:1480646/93‑1 (MQ=255)

                                                                                ggCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAg                <  2:3199997/100‑1 (MQ=255)

                                                                                 gCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGa               >  1:2048527/1‑100 (MQ=255)

                                                                                   gATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGAcn             <  1:2554554/100‑2 (MQ=255)

                                                                                   gATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACg             <  2:1853062/100‑1 (MQ=255)

                                                                                    aTGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGa            >  1:2889725/1‑100 (MQ=255)

                                                                                     tGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGaa           >  2:967244/1‑100 (MQ=255)

                                                                                       cgtaGGTGACCGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACa         >  2:902262/4‑100 (MQ=255)

                                                                                       cGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACa         >  1:2639312/1‑100 (MQ=255)

                                                                                       cGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACa         >  1:3213500/1‑100 (MQ=255)

                                                                                       cGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACa         >  1:760487/1‑100 (MQ=255)

                                                                                          aGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGc      <  2:3208656/100‑1 (MQ=255)

                                                                                           ggTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCt     <  1:1905760/100‑1 (MQ=255)

                                                                                             tGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGa   >  2:3108725/1‑100 (MQ=255)

                                                                                             tGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGa   >  2:2207659/1‑100 (MQ=255)

                                                                                              gACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGaa  >  1:2735588/1‑100 (MQ=255)

AATCCGTAGTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAA  >  NC_000913/1146900‑1147093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`