Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,554,731 C→T M313I (ATG→ATA maeA ← malate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,554,7310CT99.2% 444.9 / ‑4.5 127M313I (ATG→ATAmaeAmalate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (62/64);  total (63/64)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

Only 100 of 127 total aligned reads displayed.

ATCTTGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAG  >  NC_000913/1554636‑1554826
                                                                                               |                                                                                               
aTCTTGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTAttt                                                                                             >  2:1415188/1‑100 (MQ=255)
  cTTGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCg                                                                                           <  2:3136338/100‑1 (MQ=255)
  cTTGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCg                                                                                           <  2:1529852/100‑1 (MQ=255)
   ttGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCgg                                                                                          <  1:181748/100‑1 (MQ=255)
    tGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGc                                                                                         <  1:767273/100‑1 (MQ=255)
    tGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGc                                                                                         >  2:2397350/1‑100 (MQ=255)
     gtcagtcaGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCa                                                                                        <  1:1284258/100‑1 (MQ=255)
      tcagtcagCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCaa                                                                                       >  1:1151711/1‑100 (MQ=255)
       cagtcagCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAAt                                                                                      >  1:2975383/1‑100 (MQ=255)
       cagtcagAAAGACAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTCCGCGCTGGGTCTGGGAGATGATTATTTCGGCact                                                                                      >  2:1770355/1‑98 (MQ=255)
        agtcagCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATg                                                                                     <  1:2397479/100‑1 (MQ=255)
        agtcagCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATg                                                                                     <  1:2394705/100‑1 (MQ=255)
        agtcagCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATg                                                                                     >  1:689614/1‑100 (MQ=255)
         gtcagCAAGCCAAAGCGATCGACCCTAAAGACTTTCTGCCGCGACGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATg                                                                                     <  2:1523707/99‑1 (MQ=255)
         gtcagCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGc                                                                                    <  1:216961/100‑1 (MQ=255)
          tcagCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGcc                                                                                   <  2:2715377/100‑1 (MQ=255)
          tcagCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGcc                                                                                   <  1:1145001/100‑1 (MQ=255)
            agCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGc                                                                                 >  1:3031145/1‑100 (MQ=255)
             gCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCa                                                                                >  2:2720091/1‑100 (MQ=255)
             gCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCa                                                                                >  2:3223757/1‑100 (MQ=255)
             gCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCa                                                                                >  2:3250154/1‑100 (MQ=255)
               aaGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATc                                                                              <  1:790759/100‑1 (MQ=255)
                aGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATcc                                                                             <  2:3214943/100‑1 (MQ=255)
                 gCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATccc                                                                            >  2:2307821/1‑100 (MQ=255)
                 gCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATccc                                                                            >  2:1350267/1‑100 (MQ=255)
                 gCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATccc                                                                            >  2:961011/1‑100 (MQ=255)
                  ccAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCTTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCg                                                                           >  2:70676/1‑100 (MQ=255)
                  ccAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCg                                                                           >  2:1894432/1‑100 (MQ=255)
                  ccAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCg                                                                           >  1:3104233/1‑100 (MQ=255)
                   cAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGc                                                                          >  1:169704/1‑100 (MQ=255)
                   cAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGc                                                                          >  1:934/1‑100 (MQ=255)
                   cAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGc                                                                          <  2:3321708/100‑1 (MQ=255)
                        cGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAAc                                                                     <  2:2451737/100‑1 (MQ=255)
                             gACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCGTTCGCGATGGGTCTGGGGGATGATTATTTCGGCGATGACGCATTCCGCTGa                                                                       >  2:3018854/1‑93 (MQ=255)
                             gACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTgcg                                                                >  2:740627/1‑100 (MQ=255)
                             gACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTgcg                                                                <  1:2623573/100‑1 (MQ=255)
                             gACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTgcg                                                                <  1:2324697/100‑1 (MQ=255)
                             gACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTgcg                                                                <  2:2167649/100‑1 (MQ=255)
                               ccATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGcc                                                              >  2:1605850/1‑100 (MQ=255)
                                cATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCa                                                             <  1:1753578/100‑1 (MQ=255)
                                cATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCa                                                             >  1:1551014/1‑100 (MQ=255)
                                 aTAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGCGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCaa                                                            >  2:856342/1‑100 (MQ=255)
                                 aTAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCaa                                                            <  1:2897473/100‑1 (MQ=255)
                                 aTAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCaa                                                            >  1:264338/1‑100 (MQ=255)
                                   aaaGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAgg                                                          <  1:2887782/100‑1 (MQ=255)
                                    aaGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAgg                                                          >  1:437143/1‑99 (MQ=255)
                                    aaGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGa                                                         <  2:2565381/100‑1 (MQ=255)
                                     aGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGaa                                                        <  1:1558879/100‑1 (MQ=255)
                                     aGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGaa                                                        >  1:2726791/1‑100 (MQ=255)
                                      gACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAg                                                       <  1:1590289/100‑1 (MQ=255)
                                       aCTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGa                                                      <  2:380525/100‑1 (MQ=255)
                                       aCTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGa                                                      <  1:2345237/100‑1 (MQ=255)
                                        cTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGAc                                                     <  2:3104216/100‑1 (MQ=255)
                                        cTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCACTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGAc                                                     <  2:2749115/100‑1 (MQ=255)
                                          ttCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGa                                                   <  1:383678/100‑1 (MQ=255)
                                           tCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGAt                                                  <  1:2988034/100‑1 (MQ=255)
                                              gccgcgccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGAtttt                                               >  2:331032/1‑100 (MQ=255)
                                              gccgcgccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGAtttt                                               >  1:2199903/1‑100 (MQ=255)
                                              gccgcgccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGAtttt                                               >  2:2731852/1‑100 (MQ=255)
                                              gccgcgccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGAtttt                                               >  2:1720042/1‑100 (MQ=255)
                                                cgcgccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGAtttttt                                             >  2:932061/1‑100 (MQ=255)
                                                cgcgccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGAtttttt                                             <  2:3031128/100‑1 (MQ=255)
                                                cgcgccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGAtttttt                                             <  1:430611/100‑1 (MQ=255)
                                                  cgccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTtc                                           <  1:851068/100‑1 (MQ=255)
                                                   tctgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGTGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTtct                                          <  1:848919/97‑1 (MQ=255)
                                                   ggcgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTAATTCGGCAATGCTTCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTtc                                           <  2:2441602/97‑1 (MQ=255)
                                                      gcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGc                                       <  1:1993543/100‑1 (MQ=255)
                                                      gcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGc                                       <  1:589247/100‑1 (MQ=255)
                                                       cTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCt                                      <  2:840690/100‑1 (MQ=255)
                                                        ttCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCtt                                     <  1:1350271/100‑1 (MQ=255)
                                                        ttCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCtt                                     <  1:639911/100‑1 (MQ=255)
                                                        ttCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCtt                                     <  1:3250172/100‑1 (MQ=255)
                                                         tCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTa                                    >  1:1492367/1‑100 (MQ=255)
                                                         tCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTa                                    >  1:257400/1‑100 (MQ=255)
                                                         tCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTa                                    >  1:854137/1‑100 (MQ=255)
                                                          ccTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTaa                                   >  1:1405991/1‑100 (MQ=255)
                                                            tCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACt                                 <  1:3313724/100‑1 (MQ=255)
                                                                 tAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGacca                            >  1:1613846/1‑100 (MQ=255)
                                                                   aTCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCACTGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGaccacc                          >  2:1117746/1‑100 (MQ=255)
                                                                     ccTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTg                        >  1:1121475/1‑100 (MQ=255)
                                                                      cTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGc                       >  1:1598218/1‑100 (MQ=255)
                                                                      cTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGACGCATCCCGCTGAACCTGCGCCAAGGAAGAAGAGTTTTTTCTCGCATAACTGACCACCTGc                       >  2:2543990/1‑100 (MQ=255)
                                                                       ttCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGcc                      <  1:2397361/100‑1 (MQ=255)
                                                                          gcgcTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCcgcg                   <  1:2720104/100‑1 (MQ=255)
                                                                          gcgcTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCcgcg                   >  1:482442/1‑100 (MQ=255)
                                                                             cTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCgg                >  2:176715/1‑100 (MQ=255)
                                                                             cTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCgg                <  1:3001411/100‑1 (MQ=255)
                                                                              tGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGc               >  1:1543246/1‑100 (MQ=255)
                                                                              tGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGc               <  2:673320/100‑1 (MQ=255)
                                                                                ggTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCtt             <  2:2666652/100‑1 (MQ=255)
                                                                                ggTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCt              <  2:2185827/99‑1 (MQ=255)
                                                                                 gTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTtg            >  1:1047364/1‑100 (MQ=255)
                                                                                     gggAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTtgctgc        >  1:573827/1‑100 (MQ=255)
                                                                                     gggAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTtgctgc        <  1:2114327/100‑1 (MQ=255)
                                                                                     gggAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCCTAACTGACCACCTGCCGCGCGGCTtgctgc        >  2:1373532/1‑100 (MQ=255)
                                                                                      ggAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTtgctgc        >  2:1818690/1‑99 (MQ=255)
                                                                                        agaTGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGAt     <  2:2405162/100‑1 (MQ=255)
                                                                                         gatgatTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATc    <  1:740628/100‑1 (MQ=255)
                                                                                           tgatTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAg  >  2:366927/1‑100 (MQ=255)
                                                                                           tgatTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAg  >  1:2501683/1‑100 (MQ=255)
                                                                                               |                                                                                               
ATCTTGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAG  >  NC_000913/1554636‑1554826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: