BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,252,241	C→T	S181L (TCG→TTG)	yeiI →	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,252,241	0	C	T	97.5%	407.5 / ‑3.4	122	S181L (TCG→TTG)	yeiI	putative kinase
Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (61/58); total (63/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

ACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTCGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGAATGGGTCTTTACCCTCGCTGATGAAATC  >  NC_000913/2252144‑2252335

aCGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTtgc                                                                                              <  2:2839132/100‑1 (MQ=255)

   tATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTtgcgcg                                                                                           <  2:94136/100‑1 (MQ=255)

    aTCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGa                                                                                          <  2:8571/100‑1 (MQ=255)

      cTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGAtt                                                                                        >  2:1175741/1‑100 (MQ=255)

      cTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGAtt                                                                                        >  2:2066290/1‑100 (MQ=255)

      cTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGAtt                                                                                        >  1:1545357/1‑100 (MQ=255)

       tGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGAttt                                                                                       >  1:907133/1‑100 (MQ=255)

         gCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTAc                                                                                     >  2:175413/1‑100 (MQ=255)

             ttGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACtt                                                                                   <  1:674013/98‑1 (MQ=255)

              tGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACACAACGGGTTGCGCGATTCACTACg                                                                                 >  1:3023239/1‑99 (MQ=255)

               gCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTc                                                                               >  2:362208/1‑100 (MQ=255)

                ccAATCGAGACGATCAAACCGTGCTGGCGATTAACGCTACCCATCTGCTGGCACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTcac                                                                                <  2:664548/98‑3 (MQ=255)

                ccAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCa                                                                              >  2:3320458/1‑100 (MQ=255)

                ccAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCa                                                                              >  2:2130001/1‑100 (MQ=255)

                 cAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCAt                                                                             >  1:1445095/1‑100 (MQ=255)

                   aTCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGc                                                                           >  1:1388677/1‑100 (MQ=255)

                   aTCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGc                                                                           <  2:3051978/100‑1 (MQ=255)

                     cGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCgg                                                                         >  1:479860/1‑100 (MQ=255)

                      gagaCGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCggg                                                                        <  1:3320476/100‑1 (MQ=255)

                      gagaCGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCggg                                                                        <  2:1035072/100‑1 (MQ=255)

                       agaCGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGc                                                                       <  1:2715772/100‑1 (MQ=255)

                          cGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTg                                                                    <  2:1208983/100‑1 (MQ=255)

                           gATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTgg                                                                   >  2:1073294/1‑100 (MQ=255)

                           gATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTgg                                                                   >  1:1848709/1‑100 (MQ=255)

                           gATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTgg                                                                   <  1:3150306/100‑1 (MQ=255)

                           gATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTgg                                                                   >  2:3159198/1‑100 (MQ=255)

                           gATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTgg                                                                   <  2:1003060/100‑1 (MQ=255)

                            aTCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGt                                                                  <  1:1528124/100‑1 (MQ=255)

                            aTCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGt                                                                  >  2:612027/1‑100 (MQ=255)

                            aTCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGt                                                                  <  2:2043780/100‑1 (MQ=255)

                            aTCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGt                                                                  >  1:307936/1‑100 (MQ=255)

                               taaCCGTGCGGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACt                                                               <  1:2798366/99‑1 (MQ=255)

                               aaaCCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTgcgac                                                                >  1:912110/1‑95 (MQ=255)

                                aaCCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTg                                                              <  1:1813664/100‑1 (MQ=255)

                                aaCCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCAGTCGGGCGTGGTACta                                                              >  2:2798353/1‑99 (MQ=255)

                                 aCCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTgg                                                             <  2:752009/100‑1 (MQ=255)

                                  ccGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGc                                                            <  1:1067555/100‑1 (MQ=255)

                                  ccGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGc                                                            <  2:3162403/100‑1 (MQ=255)

                                  ccGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGc                                                            <  2:255167/100‑1 (MQ=255)

                                   cGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCt                                                           <  1:2743936/100‑1 (MQ=255)

                                   cGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCt                                                           <  1:2066298/100‑1 (MQ=255)

                                        tGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTg                                                      <  1:728126/100‑1 (MQ=255)

                                        tGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTg                                                      >  2:2120850/1‑100 (MQ=255)

                                        tGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTg                                                      <  1:3010232/100‑1 (MQ=255)

                                        tGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTg                                                      <  2:2168297/100‑1 (MQ=255)

                                         ggCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGc                                                     <  1:3125812/100‑1 (MQ=255)

                                         ggCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGc                                                     >  1:1325363/1‑100 (MQ=255)

                                         ggCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGc                                                     >  2:2964714/1‑100 (MQ=255)

                                          gCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCa                                                    <  1:1047387/100‑1 (MQ=255)

                                           cGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCaa                                                   >  1:765919/1‑100 (MQ=255)

                                             aTTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAAcc                                                 <  1:644183/100‑1 (MQ=255)

                                               tAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCt                                                <  2:426766/99‑1 (MQ=255)

                                               tAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTg                                               <  2:2104447/100‑1 (MQ=255)

                                               tAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTg                                               <  1:820213/100‑1 (MQ=255)

                                               tAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTCGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTg                                               <  2:1120877/100‑1 (MQ=255)

                                                aaCGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGa                                              >  2:2235292/1‑100 (MQ=255)

                                                aaCGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGa                                              >  1:2222075/1‑100 (MQ=255)

                                                   gATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAg                                           <  2:350047/100‑1 (MQ=255)

                                                       cccATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGa                                       >  2:2984137/1‑100 (MQ=255)

                                                       cccATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGa                                       >  1:3071655/1‑100 (MQ=255)

                                                       cccATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTCGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGa                                       >  1:2203313/1‑100 (MQ=255)

                                                         cATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAgg                                     >  1:2596211/1‑100 (MQ=255)

                                                          aTCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGgc                                    <  1:2130011/100‑1 (MQ=255)

                                                           tCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGgcg                                   >  1:569947/1‑100 (MQ=255)

                                                            ctgctgGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGgcgc                                  >  2:223325/1‑100 (MQ=255)

                                                             tgctgGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAgg                                     >  1:1638759/1‑96 (MQ=255)

                                                              gctgGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTg                                >  2:648994/1‑100 (MQ=255)

                                                              gctgGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTg                                <  1:1312930/100‑1 (MQ=255)

                                                               ctgGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTgg                               >  1:2121183/1‑100 (MQ=255)

                                                               ctgGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTgg                               <  1:2520132/100‑1 (MQ=255)

                                                               ctgGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTgg                               <  1:1015439/100‑1 (MQ=255)

                                                               ctgGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTgg                               >  1:2633595/1‑100 (MQ=255)

                                                                tgGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGa                              >  2:1003592/1‑100 (MQ=255)

                                                                tgGAACAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGa                              >  2:1633983/1‑100 (MQ=255)

                                                                   aaCAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGAATg                           <  2:2987328/100‑1 (MQ=255)

                                                                   aaCAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGAATg                           <  2:1270901/100‑1 (MQ=255)

                                                                   aaCAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCCTCACGCGGGCGTGGCACTGGCTGATTGCAAcc                                                 >  2:2916871/1‑78 (MQ=255)

                                                                     cAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGAATggg                         <  1:3031339/100‑1 (MQ=255)

                                                                     cAGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGAATggg                         <  2:907131/100‑1 (MQ=255)

                                                                      aGTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGAATGGGt                        >  2:1279916/1‑100 (MQ=255)

                                                                       gTTGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGAATGGGTc                       <  1:1532701/100‑1 (MQ=255)

                                                                        ttGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGAATGGGTCt                      <  1:803141/100‑1 (MQ=255)

                                                                         tGACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGAATGGGTCtt                     >  2:685817/1‑100 (MQ=255)

                                                                          gACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGAATGGGTCttt                    <  2:2541823/100‑1 (MQ=255)

                                                                          gACACCGCAACTACTGAACGGGTTGCGCGATTTACTTCGCCATGCGGGCGTGGGACTCGCTGATTGCAACCTGACACCCGAGGCGCTGGAATGGGTCtt                     >  2:2799362/1‑99 (MQ=255)

                                                                           acacCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGAATGGGTCTTTa                   >  1:2787680/1‑100 (MQ=255)

                                                                             acCGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGAATGCGTCTTTAc                  <  2:912108/99‑1 (MQ=255)

                                                                               cGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGAATGGGTCTTTACCCt               >  1:1030667/1‑100 (MQ=255)

                                                                               cGCAACTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGAATGGGTCTTTACCCt               <  2:3262670/100‑1 (MQ=255)

                                                                               cGCAACTACTGAACGGGTTGAGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGAATGGGTCTTTACCCt               >  2:2055921/1‑100 (MQ=255)

                                                                                  aaCTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGAATGGGTCTTTACCCTCGc            <  1:880404/100‑1 (MQ=255)

                                                                                  aaCTACTGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGAATGGGTCTTTACCCTCGc            <  1:507223/100‑1 (MQ=255)

                                                                                   actactGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGAATGGGTCTTTACCCTCGCt           <  2:624901/100‑1 (MQ=255)

                                                                                    ctactGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGAATGGGTCTGTACCCTCGCtg          >  2:1019595/1‑100 (MQ=255)

                                                                                     tactGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGAATGGGTCTTTACCCTCGCtga         >  2:716243/1‑100 (MQ=255)

                                                                                        tGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGAATGGGTCTTTACCCTCGCtgatga      <  1:2516291/100‑1 (MQ=255)

                                                                                        tGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGAATGGGTCTTTACCCTCGCtgatga      >  1:127988/1‑100 (MQ=255)

                                                                                        tGAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGAATGGGTCTTTACCCTCCCtgatga      >  2:3130363/1‑100 (MQ=255)

                                                                                         gAACGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGAATGGGTCTTTACCCTCGCtgatga      <  2:3149287/99‑1 (MQ=255)

                                                                                            cGGGTTGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGAATGGGTCTTTACCCTCGCTGATGAAATc  >  2:2060027/1‑100 (MQ=255)

ACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAACTACTGAACGGGTCGCGCGATTTACTTCGTCATGCGGGCGTGGTACTGGCTGATTGCAACCTGACAGCCGAGGCGCTGGAATGGGTCTTTACCCTCGCTGATGAAATC  >  NC_000913/2252144‑2252335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`