Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,194,516 A→G K551K (AAA→AAG yqiK → PHB family membrane protein, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,194,5160AG96.3% 456.0 / 3.6 135K551K (AAA→AAGyqiKPHB family membrane protein, function unknown
Reads supporting (aligned to +/- strand):  ref base A (2/3);  new base G (79/51);  total (81/54)
Fisher's exact test for biased strand distribution p-value = 3.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 139 total aligned reads displayed.

CGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGT  >  NC_000913/3194419‑3194612
                                                                                                 |                                                                                                
cGCTGATTGCCTCCTTGCTCAATGAAATTGGCGTTTCAGGCTGCTCACTGGCGGCATTGACTTCACCCTTACCCTCAACAACTCCCGTCGAAGAAAAGGc                                                                                                <  2:2078833/100‑1 (MQ=255)
 gCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCa                                                                                               >  1:910481/1‑100 (MQ=255)
  cTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCag                                                                                              <  2:217046/100‑1 (MQ=255)
  cTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCag                                                                                              <  2:75606/100‑1 (MQ=255)
    gATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCagag                                                                                            <  1:1963814/100‑1 (MQ=255)
    gATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCagag                                                                                            >  1:2075262/1‑100 (MQ=255)
      ttGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTa                                                                                          <  2:1636356/100‑1 (MQ=255)
      ttGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTa                                                                                          <  1:2721499/100‑1 (MQ=255)
       tGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTaa                                                                                         >  1:515249/1‑100 (MQ=255)
         aCTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAAc                                                                                       >  2:1810257/1‑100 (MQ=255)
               tGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCtt                                                                                 <  1:133855/100‑1 (MQ=255)
                tcTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCTGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTc                                                                                <  1:3189094/99‑1 (MQ=255)
                gCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTc                                                                                >  2:3266902/1‑100 (MQ=255)
                gCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTc                                                                                >  1:229380/1‑100 (MQ=255)
                   cAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAg                                                                             >  1:2086297/1‑100 (MQ=255)
                   cAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTcaga                                                                             >  2:3189076/1‑97 (MQ=255)
                    aaTGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAgg                                                                            >  2:253487/1‑100 (MQ=255)
                    aaTGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAgg                                                                            <  2:1658658/100‑1 (MQ=255)
                    aaTGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAgg                                                                            <  1:1920768/100‑1 (MQ=255)
                    aaTGAAATTGGCGTTTCAGGCGGCGCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAgg                                                                            <  1:1513184/100‑1 (MQ=255)
                     aTGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGa                                                                           >  2:2828642/1‑100 (MQ=255)
                      tGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGaa                                                                          <  1:1262722/100‑1 (MQ=255)
                       gAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGaat                                                                         >  2:2959793/1‑100 (MQ=255)
                        acaTTGGCGTTTCAGGCGGCTCACTGGCGGCCTTGACTTCACGCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGaata                                                                        >  1:2260851/3‑100 (MQ=255)
                          aTTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTGAACATCTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAgg                                                                            <  1:677485/94‑1 (MQ=255)
                          aTTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGaataat                                                                      <  2:3025818/100‑1 (MQ=255)
                           ttGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATc                                                                     <  1:2531732/100‑1 (MQ=255)
                             ggCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATCATccc                                                                   >  1:1049369/1‑100 (MQ=255)
                             ggCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATccc                                                                   <  2:1442980/100‑1 (MQ=255)
                               cGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTg                                                                 >  1:3061358/1‑100 (MQ=255)
                               cGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTg                                                                 <  2:1230185/100‑1 (MQ=255)
                                gttttgggcggcTCACTGGCGGCATTGACTTCACCCTGAACCGCAACAACGCCGGGCGAAGAAAAGGCAGAGTAAGCCCGCTTCCAGGAATAGTCCCTgg                                                                <  2:55464/94‑1 (MQ=255)
                                gTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTgg                                                                >  1:3147941/1‑100 (MQ=255)
                                  ttCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGcc                                                              >  1:1126540/1‑100 (MQ=255)
                                  ttCAGGCGGCTCACTGGCGGCATTGAATTCACCCTTAACCTCAACAACTCCCGTCGAAGACAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGcc                                                              <  2:1330876/100‑1 (MQ=255)
                                   tCAGGCGGCTGACGGGCGGGATTGGAGTGACGCTTAACCGCAGCTGCTCCCGTCGAAGAAAAGGCAGAGTAAACGCGCTTCCCGGAATAATCCCTg                                                                 <  2:729126/96‑1 (MQ=255)
                                    naGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACATCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCa                                                            >  2:1803787/2‑100 (MQ=255)
                                    cAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCa                                                            >  2:1976561/1‑100 (MQ=255)
                                         ggcTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAg                                                       >  2:1528418/1‑100 (MQ=255)
                                         ggcTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAg                                                       >  1:2290285/1‑100 (MQ=255)
                                          gcTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGc                                                      >  2:2403629/1‑100 (MQ=255)
                                          gcTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGc                                                      >  2:1509356/1‑100 (MQ=255)
                                           cTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGcc                                                     >  2:1007995/1‑100 (MQ=255)
                                             cACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCgg                                                   >  2:2689057/1‑100 (MQ=255)
                                             cACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCgg                                                   <  1:3230605/100‑1 (MQ=255)
                                              aCTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCggg                                                  <  2:1187180/100‑1 (MQ=255)
                                               cTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCgggg                                                 >  1:2051293/1‑100 (MQ=255)
                                                tGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGa                                                >  1:1242885/1‑100 (MQ=255)
                                                 ggcggcATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCgggg                                                 >  2:2971703/1‑98 (MQ=255)
                                                   cggcATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAttt                                             >  2:3135391/1‑100 (MQ=255)
                                                     gcATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAttttt                                           >  1:1247632/1‑100 (MQ=255)
                                                      cATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAtttttt                                          >  1:2183442/1‑100 (MQ=255)
                                                      cATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAtttttt                                          >  2:1181371/1‑100 (MQ=255)
                                                      cATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAttttt                                           >  2:2513018/1‑99 (MQ=255)
                                                       aTTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTta                                         <  1:493079/100‑1 (MQ=255)
                                                         tGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTtata                                       >  1:2631955/1‑100 (MQ=255)
                                                         tGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTtata                                       >  1:2953219/1‑100 (MQ=255)
                                                            cTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCt                                    <  2:910479/100‑1 (MQ=255)
                                                            cTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAAAAAAAAAAAAAAAAAGCCGGGGATTTTTTATATCt                                    <  2:1422878/100‑1 (MQ=255)
                                                               cACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCg                                 >  1:813106/1‑100 (MQ=255)
                                                                aCCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTttatttttgt                                >  2:3183304/1‑94 (MQ=38)
                                                                aCCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGt                                <  1:314029/100‑1 (MQ=255)
                                                                 cccTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGtt                               >  1:2249126/1‑100 (MQ=255)
                                                                 cccTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGtt                               >  1:1981089/1‑100 (MQ=255)
                                                                 cccTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGtt                               >  2:1448222/1‑100 (MQ=255)
                                                                  ccTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTc                              >  1:3312174/1‑100 (MQ=255)
                                                                   cTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTcc                             >  1:1706015/1‑100 (MQ=255)
                                                                     tAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGc                           <  1:1550623/100‑1 (MQ=255)
                                                                     tAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGc                           <  1:620271/100‑1 (MQ=255)
                                                                      aaCCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCt                          >  1:1753346/1‑100 (MQ=255)
                                                                      aaCCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCt                          <  1:1989896/100‑1 (MQ=255)
                                                                      aaCCGCAACAGCGCCCGTCGAAGAAAAGGCAGAGTAAACCCGCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCt                          <  2:2242070/100‑1 (MQ=25)
                                                                       aCCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTa                         >  1:872870/1‑100 (MQ=255)
                                                                       aCCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTa                         >  2:2877222/1‑100 (MQ=255)
                                                                        ccTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTaa                        >  2:2040191/1‑100 (MQ=255)
                                                                         cTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTaaa                       >  1:2706807/1‑100 (MQ=255)
                                                                          tCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTaaaa                      <  1:1810262/100‑1 (MQ=255)
                                                                           caacaaCTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTaaaaa                     >  1:170915/1‑100 (MQ=255)
                                                                           caacaaCTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTaaaaa                     >  1:1493004/1‑100 (MQ=255)
                                                                           caacaaCTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTaaaaa                     >  2:2616137/1‑100 (MQ=255)
                                                                            aacaacTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAg                    <  1:1361873/100‑1 (MQ=255)
                                                                              caacTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGt                   <  2:823203/99‑1 (MQ=255)
                                                                               aacTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGc                 <  1:2816466/100‑1 (MQ=255)
                                                                                acTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCa                <  2:1247628/100‑1 (MQ=255)
                                                                                acTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCa                <  1:941816/100‑1 (MQ=255)
                                                                                  tCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCaaa              <  2:347602/100‑1 (MQ=255)
                                                                                   cccGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAAt             <  2:1242881/100‑1 (MQ=255)
                                                                                    ctgTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATg            <  1:596933/98‑1 (MQ=255)
                                                                                    ccGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATg            >  1:2556448/1‑100 (MQ=255)
                                                                                     cGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGa           >  1:1985944/1‑100 (MQ=255)
                                                                                       tCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATc         >  2:2465719/1‑100 (MQ=255)
                                                                                        cGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCa        <  1:2959810/100‑1 (MQ=255)
                                                                                          aagaaAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAgg      >  2:2410154/1‑100 (MQ=255)
                                                                                           agaaAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTCAAAAGTGCAAATGAtaa        >  1:1737921/1‑95 (MQ=255)
                                                                                           agaaAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAgg      >  1:2487793/1‑99 (MQ=255)
                                                                                           agaaAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgt     >  2:1019845/1‑100 (MQ=255)
                                                                                             aaaaaGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttg   >  1:3271920/1‑100 (MQ=255)
                                                                                              aaagGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttgt  >  2:51212/1‑100 (MQ=255)
                                                                                              aaagGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttgt  >  2:1030903/1‑100 (MQ=255)
                                                                                              aaagGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttgt  >  1:1070785/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGT  >  NC_000913/3194419‑3194612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: