BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	632,971	C→T	A169A (GCG→GCA)	ybdH ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	632,971	0	C	T	97.7%	443.6 / ‑1.1	130	A169A (GCG→GCA)	ybdH	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (63/64); total (65/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 131 total aligned reads displayed.

ATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGT  >  NC_000913/632875‑633067

  tattGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCcag                                                                                             <  1:1239932/100‑1 (MQ=255)

  tattGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCcag                                                                                             >  1:2582760/1‑100 (MQ=255)

  tattGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCcag                                                                                             <  2:1847258/100‑1 (MQ=255)

   attGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCcagc                                                                                            <  2:88428/100‑1 (MQ=255)

     tGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCcagcag                                                                                          >  1:1942771/1‑100 (MQ=255)

     tGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAACCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCcagcag                                                                                          <  2:2819909/100‑1 (MQ=255)

      gATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCcagcag                                                                                          >  2:2311152/1‑99 (MQ=255)

       aTCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGat                                                                                        >  2:1786264/1‑100 (MQ=255)

        tccgatctCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGata                                                                                       <  2:395325/93‑1 (MQ=255)

         ccccAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGatat                                                                                      >  2:618920/1‑100 (MQ=255)

           cgatctgCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATAttg                                                                                    <  1:1416635/94‑1 (MQ=255)

             aGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATAttgtt                                                                                  >  1:2430206/1‑100 (MQ=255)

             aGTCACACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATAttgtt                                                                                  <  2:1037954/100‑1 (MQ=255)

              gTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATAttgttg                                                                                 <  2:1379859/100‑1 (MQ=255)

                cGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGataagatcgga                                                                               >  1:395326/1‑92 (MQ=255)

                cGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCg                                                                               <  2:3016409/100‑1 (MQ=255)

                cGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCg                                                                               <  2:733296/100‑1 (MQ=255)

                 gCACGGTTAGCGGCAACGTTTCTGGTTGTGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCgg                                                                              >  2:11638/1‑100 (MQ=255)

                 gCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATAttgagatcg                                                                              >  2:1416631/1‑94 (MQ=255)

                 gCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCgg                                                                              <  1:1370506/100‑1 (MQ=255)

                 gCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCgg                                                                              <  2:3239934/100‑1 (MQ=255)

                  cACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGt                                                                             <  1:1483401/100‑1 (MQ=255)

                   aCGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTg                                                                            <  2:940895/100‑1 (MQ=255)

                    cGGTTAGCGGCAACGTTTCTGGTTTCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATAGTGTTGCGGTg                                                                            <  2:1235182/99‑1 (MQ=255)

                    cGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGc                                                                           <  2:3288766/100‑1 (MQ=255)

                     ggTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCa                                                                          <  2:3217365/100‑1 (MQ=255)

                     ggTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCa                                                                          <  2:1535154/100‑1 (MQ=255)

                            ggCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAgg                                                                    >  2:57080/1‑99 (MQ=255)

                            ggCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGAGATTGTTGCGGTGCATTGAGGa                                                                   >  1:1407036/1‑100 (MQ=255)

                             gCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGAt                                                                  >  2:63334/1‑100 (MQ=255)

                               aaCGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATaa                                                                <  1:3085493/100‑1 (MQ=255)

                               aaCGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATaa                                                                >  1:2527752/1‑100 (MQ=255)

                                aCGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATCTTGTTGCGGTGCATTGAGGATaa                                                                >  2:2220054/1‑99 (MQ=255)

                                 cGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAAtc                                                              <  1:1524779/100‑1 (MQ=255)

                                 cGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAAtc                                                              <  2:2726701/100‑1 (MQ=255)

                                  gTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAAtct                                                             >  1:423366/1‑100 (MQ=255)

                                    ttCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTcc                                                           <  2:1704225/100‑1 (MQ=255)

                                     tCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCg                                                          <  1:1340404/100‑1 (MQ=255)

                                     tCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCg                                                          >  1:634173/1‑100 (MQ=255)

                                     tCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCg                                                          <  1:618101/100‑1 (MQ=255)

                                      cTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCgg                                                         >  2:2662773/1‑100 (MQ=255)

                                       tGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGt                                                        <  1:1362139/100‑1 (MQ=255)

                                       tGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGt                                                        <  1:2777679/100‑1 (MQ=255)

                                            gCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTccac                                                   <  2:835719/100‑1 (MQ=255)

                                            gCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGAAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTcca                                                    >  1:2325026/1‑99 (MQ=255)

                                             cGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTccacc                                                  <  2:2189170/100‑1 (MQ=255)

                                             cGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTccacc                                                  <  1:612056/100‑1 (MQ=255)

                                             cGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTccacc                                                  <  2:2519065/100‑1 (MQ=255)

                                             cGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTccacc                                                  <  1:394659/100‑1 (MQ=255)

                                               gAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAg                                                <  1:3268811/100‑1 (MQ=255)

                                               gAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTACGGTGCATTGAGGATAATCTCCGGTTCCACCAg                                                <  2:516814/100‑1 (MQ=255)

                                                 gCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCa                                              >  2:2870327/1‑100 (MQ=255)

                                                 gCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCa                                              <  2:484221/100‑1 (MQ=255)

                                                  ccAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCAc                                             <  2:2950025/100‑1 (MQ=255)

                                                    aGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACTAGCACCa                                           <  1:1760167/100‑1 (MQ=255)

                                                    aGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCa                                           <  1:886745/100‑1 (MQ=255)

                                                         caccGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATaaa                                       >  1:2780694/1‑99 (MQ=255)

                                                           ccGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAAtt                                    <  1:2392404/100‑1 (MQ=255)

                                                            cGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTg                                   <  2:3174849/100‑1 (MQ=255)

                                                             gCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTgg                                  <  2:2157410/100‑1 (MQ=255)

                                                              cTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATACAATTGGc                                 >  1:816412/1‑100 (MQ=255)

                                                              cTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGc                                 <  2:3165036/100‑1 (MQ=255)

                                                                tCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGcgt                               >  1:2818500/1‑100 (MQ=255)

                                                                 cATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGGGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGcgtc                              >  1:1584278/1‑100 (MQ=255)

                                                                  aTACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGcgtcg                             >  1:2392558/1‑100 (MQ=255)

                                                                   tACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATACTCTCCGGTTCCACCAGCACCATAAAATTGGcgtcgt                            >  2:2866109/1‑100 (MQ=255)

                                                                   tACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGcgtcgt                            >  1:1822819/1‑100 (MQ=255)

                                                                   tACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGcgtcgt                            >  2:3082824/1‑100 (MQ=255)

                                                                     ccATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGtcgtcg                          >  1:3002562/1‑100 (MQ=255)

                                                                      cATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGGCGTCGa                         >  2:3150582/1‑100 (MQ=255)

                                                                       aTTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGaa                        >  1:2883267/1‑100 (MQ=255)

                                                                         ttCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGaaaa                      >  2:412426/1‑100 (MQ=255)

                                                                          tCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAAt                     <  2:8954/100‑1 (MQ=255)

                                                                           cGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAAtc                    <  2:1477254/100‑1 (MQ=255)

                                                                           cGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAAtc                    >  1:2746042/1‑100 (MQ=255)

                                                                           cGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAACCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAAtc                    >  2:2146948/1‑100 (MQ=255)

                                                                            gCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAAtct                   >  2:1723445/1‑100 (MQ=255)

                                                                            gCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTGGCGGTGCATTTAGGCTAATCTCCGGTTCCACCAGCTCCATAAAATTGGCGTCGTCGAAAAtct                   >  2:810196/1‑100 (MQ=255)

                                                                             ccAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAAtctc                  <  2:2430195/100‑1 (MQ=255)

                                                                             ccAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAAtctc                  >  1:2795397/1‑100 (MQ=255)

                                                                             ccAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAAtctc                  >  1:2872085/1‑100 (MQ=255)

                                                                             ccAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAAtctc                  >  2:2465728/1‑100 (MQ=255)

                                                                             ccAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAAtctc                  >  1:2304069/1‑100 (MQ=255)

                                                                                 cGTGTCACCGATCCCTGCCAGCAGTTATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATa               <  2:1942763/99‑1 (MQ=255)

                                                                                 cGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATaa              <  1:1725455/100‑1 (MQ=255)

                                                                                 cGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATaa              >  1:1960937/1‑100 (MQ=255)

                                                                                 cGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATaa              >  1:2604279/1‑100 (MQ=255)

                                                                                  gtgtCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAAt             >  1:703838/1‑100 (MQ=255)

                                                                                   tgtCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATg            >  1:748254/1‑100 (MQ=255)

                                                                                    gtCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGc           <  2:962019/100‑1 (MQ=255)

                                                                                    gtCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGc           <  2:2703271/100‑1 (MQ=255)

                                                                                     tCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCa          <  2:474541/100‑1 (MQ=255)

                                                                                     tCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCa          <  1:1185582/100‑1 (MQ=255)

                                                                                     tCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCa          >  1:265948/1‑100 (MQ=255)

                                                                                      cACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAg         >  2:59660/1‑100 (MQ=255)

                                                                                       aCCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAgc        >  1:931163/1‑100 (MQ=255)

                                                                                       aCCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAgc        >  1:494140/1‑100 (MQ=255)

                                                                                       aCCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAgc        <  1:2678429/100‑1 (MQ=255)

                                                                                          gATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATagatcggaaga     >  1:2686244/1‑90 (MQ=255)

                                                                                          gATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGcc     <  2:3002545/100‑1 (MQ=255)

ATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGT  >  NC_000913/632875‑633067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`