BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	780,758	T→C	intergenic (+129/‑7)	lysT → / → valT	tRNA‑Lys/tRNA‑Val

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	780,758	0	T	C	94.7%	423.8 / 5.8	131	intergenic (+129/‑7)	lysT/valT	tRNA‑Lys/tRNA‑Val
Reads supporting (aligned to +/- strand): ref base T (2/5); new base C (52/72); total (54/77)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 136 total aligned reads displayed.

AAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGT  >  NC_000913/780661‑780846

aaGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCgcag                                                                                        <  1:3206019/100‑1 (MQ=255)

aaGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCgcag                                                                                        <  1:28819/100‑1 (MQ=255)

  ggATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCgcagta                                                                                      >  1:309161/1‑100 (MQ=255)

  ggATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCgcagta                                                                                      >  1:1806694/1‑100 (MQ=255)

  ggATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCgcagta                                                                                      <  1:2957598/100‑1 (MQ=255)

   gATAACGTTGCATGAGCAACGGCTCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTAt                                                                                     >  2:957946/1‑100 (MQ=255)

     tAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCg                                                                                   <  1:3118284/100‑1 (MQ=255)

     tAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCg                                                                                   >  1:1722858/1‑100 (MQ=255)

      cgatctGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCgg                                                                                  <  2:186468/95‑1 (MQ=255)

      aaCGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCgg                                                                                  <  1:2121735/100‑1 (MQ=255)

       aCGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGGGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCggg                                                                                 >  1:2290660/1‑100 (MQ=255)

       aCGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCggg                                                                                 <  2:1485216/100‑1 (MQ=255)

       aCGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCggg                                                                                 <  2:1213378/100‑1 (MQ=255)

       aCGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCggg                                                                                 >  1:2510906/1‑100 (MQ=255)

        cGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGt                                                                                <  2:166165/100‑1 (MQ=255)

        cGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGt                                                                                <  2:2745298/100‑1 (MQ=255)

        cGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGt                                                                                <  1:883647/100‑1 (MQ=255)

        cGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTAGTAGTATCCAGCGTAGTATCgg                                                                                  >  2:3070730/1‑98 (MQ=255)

         gttgctcatgCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGt                                                                                >  2:908549/10‑99 (MQ=21)

          ttGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGa                                                                              >  1:608964/1‑100 (MQ=255)

           tGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGAt                                                                             <  1:2897056/100‑1 (MQ=255)

            gCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGAtt                                                                            <  2:344365/100‑1 (MQ=255)

            gCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGAtt                                                                            <  1:2742274/100‑1 (MQ=255)

            gCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGAtt                                                                            >  2:2251354/1‑100 (MQ=255)

            gCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGAtt                                                                            >  1:3030332/1‑100 (MQ=255)

             cATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTa                                                                           <  2:2107926/100‑1 (MQ=255)

             cATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTa                                                                           <  1:3001811/100‑1 (MQ=255)

               tGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGc                                                                         <  2:344261/100‑1 (MQ=255)

               tGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGc                                                                         <  2:753425/100‑1 (MQ=255)

               tGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGc                                                                         <  1:1474161/100‑1 (MQ=255)

               tGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAACGCAGTATCGGGTGATTAGc                                                                         <  2:2056180/100‑1 (MQ=255)

                gAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCt                                                                        >  1:633104/1‑100 (MQ=255)

                 aGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTc                                                                       <  1:666877/100‑1 (MQ=255)

                  gCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCa                                                                      <  1:3012060/100‑1 (MQ=255)

                  gCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCa                                                                      >  2:842649/1‑100 (MQ=255)

                  gCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCa                                                                      <  1:2914181/100‑1 (MQ=255)

                   cAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAg                                                                     >  1:2292222/1‑100 (MQ=255)

                    aaCGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGc                                                                    <  2:40578/100‑1 (MQ=255)

                    aaCGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGc                                                                    <  2:2781312/100‑1 (MQ=255)

                     aCGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCt                                                                   <  1:2214567/100‑1 (MQ=255)

                      cGGCCCGAACAGCGAGACGAAGCCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAg                                                                     <  2:2947980/97‑1 (MQ=37)

                       ggCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCt                                                                   >  2:2850363/1‑98 (MQ=255)

                        gCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTggg                                                                >  1:639102/1‑100 (MQ=255)

                          ccGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGgag                                                              >  2:937601/1‑100 (MQ=255)

                           cGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGgaga                                                             <  1:1163093/100‑1 (MQ=255)

                           cGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGgaga                                                             >  1:53816/1‑100 (MQ=255)

                           cGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGgaga                                                             <  1:1163160/100‑1 (MQ=255)

                           cGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGgag                                                              <  2:2733335/99‑1 (MQ=255)

                            gAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGagag                                                            <  1:1916422/100‑1 (MQ=255)

                             aaGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGc                                                           <  2:2116359/100‑1 (MQ=255)

                              aGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCa                                                          <  1:2809856/100‑1 (MQ=255)

                               gggCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCa                                                          >  2:2303461/1‑99 (MQ=255)

                               gggCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCAc                                                         <  2:2510894/100‑1 (MQ=255)

                                  cGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTc                                                      <  2:258870/100‑1 (MQ=255)

                                  cGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTc                                                      >  1:350247/1‑100 (MQ=255)

                                    agaCGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTccc                                                    <  2:1310965/100‑1 (MQ=255)

                                    agaCGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTccc                                                    <  2:1908896/100‑1 (MQ=255)

                                    agaCGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTc                                                      <  2:824331/98‑1 (MQ=255)

                                      aCGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCtt                                                  <  1:2439826/100‑1 (MQ=255)

                                       cGAAGTCGAGTCATCCTGCACGACCCACCACTAACGTAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTa                                                 <  2:96658/100‑1 (MQ=255)

                                       cGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTa                                                 >  2:3254479/1‑100 (MQ=255)

                                       cGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTa                                                 >  1:672037/1‑100 (MQ=255)

                                        gAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTAc                                                >  2:2850888/1‑100 (MQ=255)

                                         aaGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACa                                               >  2:1747023/1‑100 (MQ=255)

                                          aGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTCTCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACaa                                              >  2:3181843/1‑100 (MQ=255)

                                           gTCGAGTCATCCTGCACGACCCACCACTCACATAGATAGTCGTAGTATCCAGCGCAGTCTCGGATCGTTAGCTCAGCTGGGAGAGCACCTCCGTTACAag                                             >  2:1613593/1‑100 (MQ=18)

                                            tCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAagg                                            <  1:2695613/100‑1 (MQ=255)

                                            tCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAagg                                            <  1:965328/100‑1 (MQ=255)

                                             cGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAagga                                           <  1:3215583/100‑1 (MQ=255)

                                             cGAGTCATCCTGCACGACACACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAagga                                           >  2:2794227/1‑100 (MQ=255)

                                               aGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAaggagg                                         >  2:1081176/1‑100 (MQ=255)

                                               aGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAaggagg                                         <  2:633103/100‑1 (MQ=255)

                                                 tCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAgggg                                       <  1:2079307/100‑1 (MQ=255)

                                                    tCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTc                                    <  2:2327683/100‑1 (MQ=255)

                                                    tCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTc                                    <  1:671821/100‑1 (MQ=255)

                                                    tCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTc                                    >  1:563338/1‑100 (MQ=255)

                                                     ccTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTcg                                   >  1:2660608/1‑100 (MQ=255)

                                                     ccTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTCCAAGGAGGGGGGcg                                   >  1:1012629/1‑100 (MQ=255)

                                                        gCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTcggcg                                >  2:2670408/1‑100 (MQ=255)

                                                           cGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGtt                             >  1:914950/1‑100 (MQ=255)

                                                           cGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGtt                             <  1:2514843/100‑1 (MQ=255)

                                                            gACCCACCACTAACATTGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTc                            <  1:944324/100‑1 (MQ=255)

                                                            gACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTc                            <  1:1499716/100‑1 (MQ=255)

                                                             aCCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCg                           >  1:3243692/1‑100 (MQ=255)

                                                             aCCCACCACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCg                           <  1:1849562/100‑1 (MQ=255)

                                                                caccacTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATc                        >  1:2628247/1‑100 (MQ=255)

                                                                caccacTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATc                        <  2:241674/100‑1 (MQ=255)

                                                                 accacTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATcc                       <  2:377672/100‑1 (MQ=255)

                                                                 accacTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATcc                       <  1:212886/100‑1 (MQ=255)

                                                                  ccacTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATccc                      >  2:1522628/1‑100 (MQ=255)

                                                                  ccacTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATccc                      <  2:91430/100‑1 (MQ=255)

                                                                  ccacTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATccc                      >  2:2612274/1‑100 (MQ=255)

                                                                      tAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGtca                  <  1:638087/100‑1 (MQ=255)

                                                                       aaCATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGtcat                 <  2:1327421/100‑1 (MQ=255)

                                                                       aaCATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGtcat                 >  1:888703/1‑100 (MQ=255)

                                                                       aaCATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGtcat                 >  2:2787721/1‑100 (MQ=255)

                                                                         cATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGtcatca               <  2:2386121/100‑1 (MQ=25)

                                                                          aTAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcac              <  1:1922692/100‑1 (MQ=18)

                                                                           tagttagtTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcacc             <  1:3070747/100‑1 (MQ=255)

                                                                             gttagttGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcaccca           >  1:1516973/1‑100 (MQ=11)

AAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGT  >  NC_000913/780661‑780846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`