Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 953,802 A→G V222A (GTA→GCA)  focA ← formate channel

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913953,8020AG96.8% 422.5 / 0.6 123V222A (GTA→GCA) focAformate channel
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base G (49/70);  total (51/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

GTTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGCTTTG  >  NC_000913/953705‑953897
                                                                                                 |                                                                                               
gTTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCa                                                                                               <  2:2925740/100‑1 (MQ=255)
 ttATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCaa                                                                                              <  1:2337714/100‑1 (MQ=255)
 ttATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCaa                                                                                              <  2:844718/100‑1 (MQ=255)
 ttATCAGTGATCAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCaa                                                                                              <  2:2793353/100‑1 (MQ=255)
  tATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAAt                                                                                             <  1:364198/100‑1 (MQ=255)
    tCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATAc                                                                                           <  1:1686187/100‑1 (MQ=255)
     ccgTGATGAATTTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATAcc                                                                                          >  1:219325/3‑100 (MQ=255)
     cAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATAcc                                                                                          >  1:3017097/1‑100 (MQ=255)
     cAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATAcc                                                                                          >  1:1910160/1‑100 (MQ=255)
     cAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATAcc                                                                                          >  2:3034782/1‑100 (MQ=255)
      aGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATAccc                                                                                         >  2:606166/1‑100 (MQ=255)
       gTGATGAAATTCATCACGGTCAGGTGAGAAAAGTTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCa                                                                                        <  1:1252272/100‑1 (MQ=255)
         gatgaAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAATCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATc                                                                                      >  2:1867830/1‑100 (MQ=255)
         gatgaAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATc                                                                                      >  1:1323851/1‑100 (MQ=255)
         gatgaAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATc                                                                                      <  2:1929553/100‑1 (MQ=255)
         gatgaAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATc                                                                                      <  1:1169487/100‑1 (MQ=255)
         gatgaAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATc                                                                                      >  2:207355/1‑100 (MQ=255)
          atgaAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCg                                                                                     <  1:2667922/100‑1 (MQ=255)
            gaAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCggg                                                                                   >  1:1338340/1‑100 (MQ=255)
            gaAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCgg                                                                                    <  1:1704972/99‑1 (MQ=255)
              aaTTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGAt                                                                                 <  1:2013500/100‑1 (MQ=255)
              aaTTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGAt                                                                                 <  1:2984836/100‑1 (MQ=255)
                    tcaCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAAc                                                                          <  2:1506525/100‑1 (MQ=255)
                      aCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACa                                                                         >  2:281268/1‑100 (MQ=255)
                       cGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACAt                                                                        >  2:1030996/1‑100 (MQ=255)
                         gTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGt                                                                      <  1:1679770/100‑1 (MQ=255)
                          tCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGCCGCGGATTGCAATACCCATCGGGATCATAAACATGtt                                                                     <  2:1798986/100‑1 (MQ=255)
                           cAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGttt                                                                    <  1:2044290/100‑1 (MQ=255)
                           cAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGtt                                                                     >  2:847668/1‑99 (MQ=255)
                            aGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTg                                                                   <  2:137733/100‑1 (MQ=255)
                            aGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTg                                                                   <  1:2108309/100‑1 (MQ=255)
                             ggTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGc                                                                  <  1:3120743/100‑1 (MQ=255)
                             ggTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGc                                                                  <  1:2490216/100‑1 (MQ=255)
                              gTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCg                                                                 <  2:2330437/100‑1 (MQ=255)
                               tGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGa                                                                <  2:2470978/100‑1 (MQ=255)
                               tGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGa                                                                <  2:2385377/100‑1 (MQ=255)
                                  gaAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATAc                                                             >  2:3080658/1‑100 (MQ=255)
                                   aaaaaTTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACt                                                            >  2:618590/1‑100 (MQ=255)
                                    aaaaTTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACtg                                                           <  1:1651867/100‑1 (MQ=255)
                                    aaaaTTTTCCGGTGCAGAACCGACTGCGGCCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACtg                                                           <  1:746977/100‑1 (MQ=255)
                                      aaTTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACtgtg                                                         <  2:80205/100‑1 (MQ=255)
                                        ttttCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCt                                                       <  2:663954/100‑1 (MQ=255)
                                        ttttCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCt                                                       <  2:1338336/100‑1 (MQ=255)
                                            ccGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCaaa                                                   <  1:1060085/100‑1 (MQ=255)
                                              ngTGCAGAACCGACTGCGGTCCAAAATTCGGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGCTCATAAACATGTTTGCGATACTGTGCTCAAAAc                                                 >  1:1198651/2‑100 (MQ=255)
                                              ggTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAAc                                                 >  2:2803422/1‑100 (MQ=255)
                                                 gCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGc                                              >  2:1412135/1‑100 (MQ=255)
                                                 gCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGc                                              <  1:1006337/100‑1 (MQ=255)
                                                 gCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGc                                              >  1:902542/1‑100 (MQ=255)
                                                   aGAACCTACTGCGGTCCAAAATTCCGGTGATGCGAAGTCGCGTATTGCAATACGCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTg                                            <  2:155586/100‑1 (MQ=255)
                                                   aGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTg                                            <  2:1456671/100‑1 (MQ=255)
                                                     aaCCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGc                                          <  1:93926/100‑1 (MQ=255)
                                                       ccGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGcaa                                        >  2:2101509/1‑100 (MQ=255)
                                                        cGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGcaac                                       >  1:2817522/1‑100 (MQ=255)
                                                         gACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGcaaca                                      <  1:941140/100‑1 (MQ=255)
                                                         gACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGcaaca                                      <  1:3055624/100‑1 (MQ=255)
                                                         gACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGcaaca                                      <  2:2411431/100‑1 (MQ=255)
                                                         gACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGcaaca                                      <  1:1039904/100‑1 (MQ=255)
                                                         gACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGcaaca                                      <  1:521384/100‑1 (MQ=255)
                                                         gACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGcaaca                                      <  1:526211/100‑1 (MQ=255)
                                                            tGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCaacaaac                                   <  1:465537/100‑1 (MQ=255)
                                                             gCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCaacaaaca                                  <  1:877212/100‑1 (MQ=255)
                                                             gCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCaacaaaca                                  >  1:2216405/1‑100 (MQ=255)
                                                                gTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATcg                               >  1:2325677/1‑100 (MQ=255)
                                                                gTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATcg                               >  1:3072905/1‑100 (MQ=255)
                                                                gTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATcg                               >  2:3302254/1‑100 (MQ=255)
                                                                gTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATcg                               >  1:2329146/1‑100 (MQ=255)
                                                                gTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATcg                               <  2:319953/100‑1 (MQ=255)
                                                                gTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATcg                               >  2:1075546/1‑100 (MQ=255)
                                                                 tCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATcgc                              <  2:3099186/100‑1 (MQ=255)
                                                                 tCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATcgc                              <  1:606167/100‑1 (MQ=255)
                                                                 tCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATcgc                              <  2:2397833/100‑1 (MQ=255)
                                                                 tCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATcgc                              <  1:604461/100‑1 (MQ=255)
                                                                 tCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATcg                               <  2:841785/99‑1 (MQ=255)
                                                                  ccAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATcgcg                             <  1:2593998/100‑1 (MQ=255)
                                                                   cAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCTATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGa                            <  2:1793370/100‑1 (MQ=255)
                                                                   cAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGa                            >  1:3058210/1‑100 (MQ=255)
                                                                    aaaaTTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGAc                           <  1:207356/100‑1 (MQ=255)
                                                                     aaaTTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGAcc                          <  1:618591/100‑1 (MQ=255)
                                                                         tCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGgca                      >  1:2791742/1‑100 (MQ=255)
                                                                         tCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGgca                      >  2:3109410/1‑100 (MQ=255)
                                                                          ccGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGgcag                     >  2:193120/1‑100 (MQ=255)
                                                                            ggggATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGgcagca                   >  1:3291882/1‑100 (MQ=255)
                                                                             gggATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCAc                  >  1:1920903/1‑100 (MQ=255)
                                                                              ggATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCAcc                 <  2:3017080/100‑1 (MQ=255)
                                                                              ggATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCAcc                 <  1:2925878/100‑1 (MQ=255)
                                                                               gATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGTGACCGGCAGCACCa                <  1:2546674/100‑1 (MQ=255)
                                                                               gATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCa                <  1:1725991/100‑1 (MQ=255)
                                                                                 tGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATa              >  2:1512684/1‑100 (MQ=255)
                                                                                  gCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATaa             <  1:3034260/100‑1 (MQ=255)
                                                                                   cGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAAt            >  2:479239/1‑100 (MQ=255)
                                                                                     aaGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGa          <  2:2733124/100‑1 (MQ=255)
                                                                                      aGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGaa         <  1:3193244/100‑1 (MQ=255)
                                                                                       gTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAc        <  1:1030998/100‑1 (MQ=255)
                                                                                         ngcgGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGc      >  1:59/2‑100 (MQ=255)
                                                                                         cgcgGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGc      >  1:402795/1‑100 (MQ=255)
                                                                                         cgcgGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTCGCAACAAACATCGCGACCGGCAGCACCATAATGAACGc      >  2:1836103/1‑100 (MQ=255)
                                                                                            ggATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGCttt   >  1:3078884/1‑100 (MQ=255)
                                                                                            ggATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGCttt   >  2:1677827/1‑100 (MQ=255)
                                                                                             gATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGCTTTg  <  2:3035094/100‑1 (MQ=255)
                                                                                                 |                                                                                               
GTTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGCTTTG  >  NC_000913/953705‑953897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: