Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,809,774:1 (T)7→8 100% coding (158/738 nt) ygaZ → putative L‑valine exporter, norvaline resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,809,7671.T82.4% 378.0 / 79.0 136coding (151/738 nt)ygaZputative L‑valine exporter, norvaline resistance protein
Reads supporting (aligned to +/- strand):  ref base . (9/15);  new base T (51/60);  total (61/75)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 158 total aligned reads displayed.

GGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGAC  >  NC_000913/2809668‑2809861
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ggATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCg                                                                                                    <  1:2173631/100‑1 (MQ=255)
 gATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑‑t                                                                                                >  1:3267067/1‑100 (MQ=255)
    gCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑‑tttt                                                                                            <  1:485853/100‑1 (MQ=255)
      aaaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑‑tttttt                                                                                          <  1:578992/100‑1 (MQ=255)
       aaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑‑ttttttt                                                                                         <  1:1221421/100‑1 (MQ=255)
         gACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑‑ttttttttc                                                                                       >  2:2260622/1‑98 (MQ=255)
         gACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑‑ttttttttc                                                                                       >  1:3814916/1‑98 (MQ=255)
          aCAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑‑ttttttttct                                                                                      <  1:3103410/100‑4 (MQ=255)
          aCAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑‑TTTTTTtctc                                                                                      <  1:1603172/100‑1 (MQ=255)
           cAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑‑ttttttttctc                                                                                     >  1:3050531/1‑96 (MQ=255)
           cAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑‑ttttttttctc                                                                                     <  1:1983601/100‑5 (MQ=255)
            aGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑‑ttttttttctcc                                                                                    <  2:1908777/100‑6 (MQ=255)
            aGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑‑ttttttttctcc                                                                                    <  1:3752420/100‑6 (MQ=255)
                  ccGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGcat                                                                                <  1:3715447/99‑1 (MQ=255)
                       tGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCAttt                                                                           >  2:2699130/1‑99 (MQ=255)
                        gTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTAt                                                                         >  1:3356164/1‑100 (MQ=255)
                         ttattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATg                                                                        >  1:353958/1‑100 (MQ=255)
                         ttattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑‑TTTTTTTCTCCTGCATCATTTATGc                                                                       <  1:2449092/100‑1 (MQ=255)
                         ttattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑‑TTTTTTTCTCCTGCATCATTTATGc                                                                       <  1:1355987/100‑1 (MQ=255)
                          tattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATgt                                                                       >  2:263406/1‑99 (MQ=255)
                           attaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCa                                                                      <  1:1497513/100‑1 (MQ=255)
                           attaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCa                                                                      >  2:190879/1‑100 (MQ=255)
                           attaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑‑TTTTTTTCTCCTGCATCATTTATGCAg                                                                     <  2:1141132/100‑1 (MQ=255)
                             taGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCacg                                                                    <  1:2583107/100‑3 (MQ=255)
                             taGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAgg                                                                    >  2:2215465/1‑100 (MQ=255)
                               gTTGTATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCTGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATAAATGATGCAggag                                                                  <  2:684253/100‑3 (MQ=255)
                               gTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGgc                                                                   >  2:1755138/1‑99 (MQ=255)
                               gTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑‑TTTTTTTCTCCTGCATAAATGATGCAggaga                                                                 <  1:2726641/100‑4 (MQ=255)
                               gTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGACTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATAAATGATGCAggag                                                                  <  2:2435787/100‑3 (MQ=255)
                                ttATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑‑TTTTTTTCTCCTGCATCATTTATGCAGGcgcg                                                                >  1:2557094/1‑100 (MQ=255)
                                   tatTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAg                                                              >  1:1794255/1‑100 (MQ=255)
                                    atTCCGGTTGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAgn                                                             <  1:1885771/100‑2 (MQ=255)
                                    atTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGc                                                             >  1:1830059/1‑100 (MQ=255)
                                     ttCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGcc                                                            <  2:1273553/100‑1 (MQ=255)
                                     ttCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGcc                                                            <  1:3437380/100‑1 (MQ=255)
                                     ttCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGcc                                                            <  2:3359700/100‑1 (MQ=255)
                                     ttCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGcc                                                            <  1:2698964/100‑1 (MQ=255)
                                         ggTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGtt                                                        <  1:115435/100‑1 (MQ=255)
                                         ggTGGCCTTTCCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGtt                                                        <  1:2664315/100‑1 (MQ=255)
                                          gTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTc                                                       <  2:1856379/100‑1 (MQ=255)
                                           tGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCg                                                      <  2:20922/100‑1 (MQ=255)
                                           tGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCg                                                      <  2:3427285/100‑1 (MQ=255)
                                             gCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTc                                                    >  1:733979/1‑100 (MQ=255)
                                             gCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTATCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTc                                                    >  1:2686589/1‑100 (MQ=255)
                                              ccTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCCGTTCGTCa                                                   >  2:3378035/1‑100 (MQ=255)
                                               cTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCAt                                                  <  1:1008603/100‑1 (MQ=255)
                                               cTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCAtt                                                 >  2:3584144/1‑100 (MQ=255)
                                                tttGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCAtt                                                 <  2:3075717/100‑1 (MQ=255)
                                                 ttGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTAc                                               >  2:249154/1‑100 (MQ=255)
                                                   gCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTAcc                                              >  1:3501532/1‑100 (MQ=255)
                                                     gTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcgc                                            >  1:3484938/1‑100 (MQ=255)
                                                     gTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcgc                                            <  2:497022/100‑1 (MQ=255)
                                                         ggTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATg                                        <  2:324303/100‑1 (MQ=255)
                                                         ggTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATg                                        >  1:1641006/1‑100 (MQ=255)
                                                         ggTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGc                                       <  1:439410/100‑1 (MQ=255)
                                                          gTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGc                                       <  1:657179/100‑1 (MQ=255)
                                                          gTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCt                                      >  1:2748806/1‑100 (MQ=255)
                                                           tCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTTTCCTTCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCt                                      >  2:1252778/1‑100 (MQ=255)
                                                           tCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCt                                      <  1:991280/100‑1 (MQ=255)
                                                            cTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTTTTTTCTGCATCATTTTTCCAGCCGCGAGCCAGTTTGTAATTACCGCGTTGc                                       >  2:1468384/1‑100 (MQ=255)
                                                            cTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTgg                                    <  1:243864/100‑1 (MQ=255)
                                                             tGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTgg                                    <  2:641940/100‑1 (MQ=255)
                                                              gAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTTTTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGc                                   >  2:2140023/1‑100 (MQ=255)
                                                              gAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGc                                   >  2:1974171/1‑100 (MQ=255)
                                                              gAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGc                                   <  2:914543/100‑1 (MQ=255)
                                                              gAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGc                                   <  1:1637349/100‑1 (MQ=255)
                                                              gAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGc                                   <  1:3352098/100‑1 (MQ=255)
                                                               aaTGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCa                                  <  1:2805451/100‑1 (MQ=255)
                                                                 tGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCTGGCTCGAGCGAGTTCGTCATTACCGCGATGGTGGCAGc                                >  2:3242178/1‑100 (MQ=255)
                                                                  gCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGGAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCa                                  >  2:2885197/1‑97 (MQ=255)
                                                                     aCCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCggg                            <  2:576539/100‑1 (MQ=255)
                                                                     aCCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCggg                            <  1:3777955/100‑1 (MQ=255)
                                                                     aCCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCggg                            >  1:3675514/1‑100 (MQ=255)
                                                                     aCCCGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCggg                            <  1:3202847/100‑1 (MQ=255)
                                                                       ccGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGag                          <  2:1324613/100‑1 (MQ=255)
                                                                       ccGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑‑TTTTTGTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGagt                         <  2:2008368/100‑1 (MQ=255)
                                                                        cGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGagt                         <  2:3351639/100‑1 (MQ=255)
                                                                        cGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGa                           >  2:333169/1‑98 (MQ=255)
                                                                        cGTCTGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCCAGCCAGTTTGTCATCACCGCGCTGCTGACAGCCg                              >  2:1774817/1‑95 (MQ=255)
                                                                            tGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCTTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTTGCAGCCGGGagtagt                      >  2:885351/1‑99 (MQ=255)
                                                                            tGGGATTCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGtt                     <  2:1617759/100‑1 (MQ=255)
                                                                            tGGGATTCTCTCCTCTCGAAAGCG‑‑‑TTTTTTTTTTTTTTTTTATTTATGCAGGCGCGAGCCAGTTTGTCATTACCGCGATGCTGGCAGCCGGGAGTTGttt                    >  2:3727000/1‑100 (MQ=255)
                                                                             gggATTCTCGCCTCTCGAAAGCG‑‑‑TTATTTGTCTACTGCACCATTTATGCAATCGCGAGCCAGTTCGTCATGACCGGCATGCTGGCAGCCGGGAGTAGttt                    >  2:2764281/1‑100 (MQ=255)
                                                                                 ttCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTgg                <  1:1259665/100‑1 (MQ=255)
                                                                                 ttCTCTCCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTgg                >  2:2484148/1‑100 (MQ=255)
                                                                                 ttCTCGCCTCGCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgtg                 <  1:1652865/99‑1 (MQ=255)
                                                                                  tctctcCTCTCGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGa               <  1:661286/100‑1 (MQ=255)
                                                                                    tctcctctcGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGAtt             >  1:303237/1‑100 (MQ=255)
                                                                                     ctcctctcGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtg            >  1:3391731/1‑100 (MQ=255)
                                                                                     ctcctctcGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtg            >  1:3394989/1‑100 (MQ=255)
                                                                                     ctcctctcGAAAGCG‑‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgc           <  1:1052989/100‑1 (MQ=255)
                                                                                      tcctctcGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgc           <  1:2810250/100‑1 (MQ=255)
                                                                                       cctctcGAAAGCGT‑‑TTTTTTTTTTTTTTTTTTTTTATGGAAGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGGAGCCGGGAGTCGTTTGTGGAtttc           >  2:2597066/1‑97 (MQ=255)
                                                                                        ctctcGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctg         <  2:3629249/100‑1 (MQ=255)
                                                                                         tctcGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctgn        <  1:71576/100‑2 (MQ=255)
                                                                                          ctcGAAAGCGT‑‑TTTTTTTTTTTTTTTTCATTTAAGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTAGCAGCCGGGACTTGTTTGTTGATtcctg         >  2:368514/1‑98 (MQ=255)
                                                                                          ctcGAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctg         >  2:2316472/1‑98 (MQ=255)
                                                                                             gAAAGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTg    <  1:501394/100‑1 (MQ=255)
                                                                                              aaaGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGa   >  1:2418909/1‑100 (MQ=255)
                                                                                               aaGCGT‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGAc  >  2:2882902/1‑100 (MQ=255)
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GGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGAC  >  NC_000913/2809668‑2809861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: