Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,866,592:1 +C 100% coding (960/993 nt) rpoS ← RNA polymerase, sigma S (sigma 38) factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,866,5881.C96.4% 582.1 / 12.7 167coding (964/993 nt)rpoSRNA polymerase, sigma S (sigma 38) factor
Reads supporting (aligned to +/- strand):  ref base . (3/3);  new base C (92/69);  total (95/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 185 total aligned reads displayed.

CATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACA  >  NC_000913/2866489‑2866682
                                                                                                    |                                                                                               
cATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAg                                                                                                  >  2:1589699/1‑100 (MQ=255)
cATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAg                                                                                                  >  2:2182572/1‑100 (MQ=255)
    ggACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGcccc                                                                                            >  1:1954767/1‑100 (MQ=255)
        cTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGccccctgc                                                                                        >  1:731368/1‑96 (MQ=255)
        cTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGccccctgc                                                                                        >  2:3251619/1‑96 (MQ=255)
          tttATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGt                                                                                       <  2:3021450/100‑1 (MQ=255)
           ttATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGtt                                                                                      <  2:1216895/100‑1 (MQ=255)
            tATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGtt                                                                                      <  2:1633015/99‑1 (MQ=255)
                gtgCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAg                                                                                 <  1:1383773/100‑1 (MQ=255)
                gtgCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAg                                                                                 >  1:2183021/1‑100 (MQ=255)
                 tgCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAgg                                                                                >  1:1247263/1‑100 (MQ=255)
                 tgCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAgg                                                                                >  1:2837153/1‑100 (MQ=255)
                  gCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGa                                                                               >  1:2492467/1‑100 (MQ=255)
                  gCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGa                                                                               >  1:3307163/1‑100 (MQ=255)
                      aGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTc                                                                           <  2:3133034/100‑1 (MQ=255)
                       gAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTcg                                                                          >  1:2152048/1‑100 (MQ=255)
                       gAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTcg                                                                          >  1:424493/1‑100 (MQ=255)
                       gAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTcg                                                                          <  2:1044646/100‑1 (MQ=255)
                       gAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTcg                                                                          >  1:1338385/1‑100 (MQ=255)
                          aaGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCgcgc                                                                       <  2:805992/100‑1 (MQ=255)
                          aaGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCgcgc                                                                       <  2:2767386/100‑1 (MQ=255)
                          aaGGCCAGCCTCGCTTGAGACTGGCCTGTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCgcgc                                                                       <  2:2441649/100‑1 (MQ=255)
                            ggCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCCCGCa                                                                      >  2:3392870/1‑99 (MQ=255)
                            ggCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCACCTGCGTTTGCAGGCTTTCGCGCa                                                                      >  2:2426296/1‑99 (MQ=255)
                              ccAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAAc                                                                   >  2:1294370/1‑100 (MQ=255)
                              ccAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAAc                                                                   >  1:1816869/1‑100 (MQ=255)
                                   cTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTCCTCGCGGAACAGCGCTTCGTTATTCAGCCCCCTGCTTTTGCAGGATTTCGCGCAAACGgcgc                                                              <  1:504508/100‑1 (MQ=255)
                                     cGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCTCGCAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAg                                                            <  2:1165636/100‑1 (MQ=255)
                                     cGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAg                                                            >  2:1195633/1‑100 (MQ=255)
                                      gCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAgg                                                           <  1:3263144/100‑1 (MQ=255)
                                      gCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAgg                                                           >  1:2353886/1‑100 (MQ=255)
                                       cTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGc                                                          >  1:2788255/1‑100 (MQ=255)
                                       cTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGc                                                          >  1:647184/1‑100 (MQ=255)
                                        ttGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGcc                                                         <  1:3815093/100‑1 (MQ=255)
                                         tGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGcc                                                         <  2:740242/99‑1 (MQ=255)
                                           agaCTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTc                                                      >  1:1601978/1‑100 (MQ=255)
                                           agaCTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTc                                                      <  2:1395770/100‑1 (MQ=255)
                                            gaCTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCa                                                     <  2:495587/100‑1 (MQ=255)
                                            gaCTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCa                                                     <  1:1277157/100‑1 (MQ=255)
                                            gaCTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCa                                                     >  1:2375247/1‑100 (MQ=255)
                                             gcTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCaa                                                    <  1:3475552/99‑1 (MQ=255)
                                             aCTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCaa                                                    <  2:1964635/100‑1 (MQ=255)
                                              cTGGCCTTTCTGACAGATGCTTACTTCCTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAAc                                                   <  2:128075/100‑1 (MQ=255)
                                              cTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAAc                                                   >  2:2902942/1‑100 (MQ=255)
                                              cTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTCGCAGGCTTTCGCGCAAACGGCGCAGGCCTTCAAc                                                   >  2:2846563/1‑100 (MQ=255)
                                               tGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAAcc                                                  <  1:3780312/100‑1 (MQ=255)
                                                ngCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAAcc                                                  >  2:2189192/2‑99 (MQ=255)
                                                ggCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCt                                                 >  1:49125/1‑100 (MQ=255)
                                                 gCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTg                                                <  1:830824/100‑1 (MQ=255)
                                                 gCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTg                                                >  2:3293959/1‑100 (MQ=255)
                                                  ccTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGa                                               >  1:206783/1‑100 (MQ=255)
                                                   cTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGaa                                              <  2:2603904/100‑1 (MQ=255)
                                                      tCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATc                                            <  1:1707038/99‑1 (MQ=255)
                                                      tCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCt                                           >  1:1667088/1‑100 (MQ=255)
                                                        tGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTgg                                         >  2:1354226/1‑100 (MQ=255)
                                                          aCAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCg                                       <  2:3327333/100‑1 (MQ=255)
                                                          aCAGATCCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGTGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCg                                       <  2:617657/100‑1 (MQ=255)
                                                           cAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGa                                      <  2:2976079/100‑1 (MQ=255)
                                                             gATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAac                                    >  1:3393899/1‑100 (MQ=255)
                                                             gATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAac                                    >  2:1223899/1‑100 (MQ=255)
                                                               tGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAacac                                  >  2:2136648/1‑100 (MQ=255)
                                                                   tacttactCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTc                              >  1:2756415/1‑100 (MQ=255)
                                                                    ccgatctCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCa                             <  1:2809307/95‑1 (MQ=255)
                                                                       tactCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACgg                          <  1:3680287/100‑1 (MQ=255)
                                                                        actCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTTGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACggg                         <  1:3141069/100‑1 (MQ=255)
                                                                        actCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACggg                         >  1:1180311/1‑100 (MQ=255)
                                                                        actCGCGGAACAGCGCTTCGAGATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACggg                         <  2:3338913/100‑1 (MQ=255)
                                                                         ctCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGt                        >  1:15334/1‑100 (MQ=255)
                                                                          tCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTg                       >  2:866257/1‑100 (MQ=255)
                                                                           cgcgGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCaagatcgg                      >  2:2809287/1‑93 (MQ=255)
                                                                           cgcgGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGa                      >  2:20469/1‑100 (MQ=255)
                                                                           cgcgGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGa                      <  1:3616929/100‑1 (MQ=255)
                                                                             cgGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAgg                    >  1:1548643/1‑100 (MQ=255)
                                                                               gAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGcc                  >  1:2546212/1‑100 (MQ=255)
                                                                               gAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGcc                  >  2:3589778/1‑100 (MQ=255)
                                                                                 aCAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCaa                >  1:3111594/1‑100 (MQ=255)
                                                                                   aGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAAtt              <  1:2040673/100‑1 (MQ=255)
                                                                                   aGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAAtt              >  2:300470/1‑100 (MQ=255)
                                                                                    gcgcTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAAttt             >  2:1840261/1‑100 (MQ=255)
                                                                                     cgcTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCagatcg            >  1:1040223/1‑95 (MQ=255)
                                                                                      gcTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCa           <  2:517399/100‑1 (MQ=255)
                                                                                      gcTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCa           >  1:950572/1‑100 (MQ=255)
                                                                                      gcTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCa           <  2:1848500/100‑1 (MQ=255)
                                                                                      gcTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCa           >  2:2527442/1‑100 (MQ=255)
                                                                                      gcTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCa           <  1:1501651/100‑1 (MQ=255)
                                                                                       cTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCAc          <  1:1096882/100‑1 (MQ=255)
                                                                                       cTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCAc          <  2:1494296/100‑1 (MQ=255)
                                                                                       cTTCGATATGCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCTGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCAc          <  1:1647973/100‑1 (MQ=255)
                                                                                          cGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGAc       >  2:1718339/1‑100 (MQ=255)
                                                                                          cGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGAcc      >  2:3813557/1‑100 (MQ=255)
                                                                                           gATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAGACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGAcc      <  1:2677359/100‑1 (MQ=255)
                                                                                           gATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGAcc      >  1:2385204/1‑100 (MQ=255)
                                                                                           gATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACATGAATCTGGCGAACACGTTCACGGGTGCGGCCAATTTCACGAcc      >  2:3533834/1‑100 (MQ=255)
                                                                                             tataCAGCCACTGCGTTTGCAGGATTTTGCGCAAACGGCGCAGGCCTTCAACCAGAATCTGGCGAACACGTTCACGGGTGAGGCCAATCTCACGACCTa    <  2:792850/99‑1 (MQ=255)
                                                                                             tatTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTa    <  2:3694552/100‑1 (MQ=255)
                                                                                             tatTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTa    <  1:3162928/100‑1 (MQ=255)
                                                                                              atTCAGCCCCCTGCCTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTAc   >  2:3572321/1‑100 (MQ=255)
                                                                                               ttCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACa  >  1:544199/1‑100 (MQ=255)
                                                                                               ttCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACa  >  1:3221002/1‑100 (MQ=255)
                                                                                               ttCAGCCCCCTGCGTCTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACa  <  1:3764137/100‑1 (MQ=255)
                                                                                                    |                                                                                               
CATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACA  >  NC_000913/2866489‑2866682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: