Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,524,458:1 +T 15.0% intergenic (+90/+23) yncE → / ← ansP ATP‑binding protein, periplasmic, function unknown/L‑asparagine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,524,4581.T15.0% 421.1 / 83.4 133intergenic (+90/+23)yncE/ansPATP‑binding protein, periplasmic, function unknown/L‑asparagine transporter
Reads supporting (aligned to +/- strand):  ref base . (72/41);  new base T (12/8);  total (84/49)
Fisher's exact test for biased strand distribution p-value = 8.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01

Only 100 of 136 total aligned reads displayed.

TGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTG  >  NC_000913/1524359‑1524555
                                                                                                    |                                                                                                  
 gcgcTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCa                                                                                                   >  1:2454388/1‑100 (MQ=255)
  cgcTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCAc                                                                                                  >  2:435244/1‑100 (MQ=255)
    cTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTCATCTGAATTTGCCCGGCCCGt                                                                                                >  2:2173147/1‑100 (MQ=255)
      gTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAg                                                                                              >  2:1639346/1‑100 (MQ=255)
      gTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAg                                                                                              >  1:3235363/1‑100 (MQ=255)
       tAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGc                                                                                             <  2:3741069/100‑1 (MQ=255)
         aTAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCg                                                                                           >  1:3719343/1‑100 (MQ=255)
          tAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCgg                                                                                          >  1:1703311/1‑100 (MQ=255)
          tAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCgg                                                                                          >  1:1943764/1‑100 (MQ=255)
           aGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCggg                                                                                         <  2:1368276/100‑1 (MQ=255)
           aGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCggg                                                                                         <  1:1674400/100‑1 (MQ=255)
             atatGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGTGCCGGGCa                                                                                      <  2:1460090/100‑1 (MQ=255)
              tatGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGTGCCGGGCaa                                                                                     <  2:1914584/100‑1 (MQ=255)
                tGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAAtt                                                                                   >  1:679050/1‑100 (MQ=255)
                tGGCGAGGGGGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGTGCCGGGCaaat                                                                                   <  2:1752321/100‑3 (MQ=255)
                 ggCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAAttt                                                                                  >  1:1767264/1‑100 (MQ=255)
                  gCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGTGCCGGGCAAattc                                                                                 <  1:1753191/100‑2 (MQ=38)
                   cGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTg                                                                                >  1:520998/1‑100 (MQ=255)
                   cGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTg                                                                                <  2:520992/100‑1 (MQ=255)
                   cGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTg                                                                                >  2:2734671/1‑100 (MQ=255)
                     aGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGTGCCGGGCAAatt                                                                                  <  2:1692037/96‑1 (MQ=38)
                      gggTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCaa                                                                             >  2:1035589/1‑100 (MQ=255)
                       ggTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAAt                                                                            >  1:552324/1‑100 (MQ=255)
                        gTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAAtt                                                                           <  1:2674476/100‑1 (MQ=255)
                         tGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTa                                                                          >  1:3354506/1‑100 (MQ=255)
                          gAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTaa                                                                         >  2:121541/1‑100 (MQ=255)
                          gAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTAGCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTaa                                                                         >  2:2350232/1‑100 (MQ=255)
                           acaaCGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAAc                                                                        >  1:3534578/3‑100 (MQ=255)
                           aaaaCGGTGAGACTTGCCTGCCTTTTATCGCGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTCCCCGGCACGTAGCCGGGCAATTTTGCAATTAAc                                                                        <  2:2298252/100‑1 (MQ=255)
                           aaaaCGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAAc                                                                        >  2:3435652/1‑100 (MQ=255)
                              aCGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCg                                                                     >  2:2624596/1‑100 (MQ=255)
                               cGGTGAGACTTGCCTGCCTTTTAAAAAAGCGTATAAGGCACTTTCGTCAGTAATCTGAATTTACGCAGCACGTACCCGGGCAAATTTGCAATTAAc                                                                        >  2:1313504/1‑96 (MQ=255)
                                ggTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGcc                                                                   >  2:1100922/1‑100 (MQ=255)
                                     gaCTTGTCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTc                                                              >  1:3447163/1‑100 (MQ=255)
                                     gaCTTGCCTGCCTATTATCACGGCGTATCCGGCACTTTAGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTc                                                              >  2:2363269/1‑100 (MQ=255)
                                        ttGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAgg                                                           <  1:292295/100‑1 (MQ=255)
                                        ttGCCTGACTTTTATCACGCCGCATCCGGCACCTTCGTCAGTAGTCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAgg                                                           >  1:3206682/1‑100 (MQ=255)
                                        ttGCCTGACTTTTATCACGCCGCATCCGGCACCTTCGTCAGTAGTCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAgg                                                           >  1:1291285/1‑100 (MQ=255)
                                         tgcctgccTTTTATCACGGCGTATCCGGGACTTGCGTCAGTAATCTGAATTTGCTCGGCACGTAGCCGGGCAATGTTGCAATTAACTCGCCGTTTCAGGc                                                          <  2:3233790/100‑1 (MQ=255)
                                          gcctgcctTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCt                                                         >  1:77137/1‑100 (MQ=255)
                                           cctgcctTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCtt                                                        >  1:897798/1‑100 (MQ=255)
                                               cctTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAac                                                    <  1:1944324/100‑1 (MQ=255)
                                               cctTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAac                                                    >  1:1133319/1‑100 (MQ=255)
                                                ctTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAaca                                                   <  2:1973911/100‑1 (MQ=255)
                                                ctTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAaca                                                   >  2:2702328/1‑100 (MQ=255)
                                                 ttttATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAacac                                                  >  1:1076284/1‑100 (MQ=255)
                                                    tATCACGGCGTATCCGGCACTTTCGTCAGCAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAAt                                               <  2:2298874/100‑1 (MQ=255)
                                                     aTCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAAtt                                              >  2:3094419/1‑100 (MQ=255)
                                                        aCGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCACTTAACTCGCCGTTTCAGGCTTAAACACCATTTcc                                           >  1:2945295/1‑100 (MQ=255)
                                                        aCGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTcc                                           >  2:2105545/1‑100 (MQ=255)
                                                          ggCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATCTCCTg                                         >  2:2753387/1‑100 (MQ=255)
                                                           gCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGt                                        <  2:834315/100‑1 (MQ=255)
                                                             gTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGtt                                       <  2:1676815/99‑1 (MQ=255)
                                                              tATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGtttt                                     >  1:743302/1‑100 (MQ=255)
                                                                 ccGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttct                                  >  1:3091279/1‑100 (MQ=255)
                                                                  cGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttctt                                 >  1:1438859/1‑100 (MQ=255)
                                                                  cGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttctt                                 <  2:2207680/100‑1 (MQ=255)
                                                                  cGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttctt                                 >  1:869272/1‑100 (MQ=255)
                                                                   ggCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttcttc                                >  2:2625915/1‑100 (MQ=255)
                                                                    gCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCa                               <  1:304090/100‑1 (MQ=255)
                                                                    gCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCa                               <  2:3458410/100‑1 (MQ=255)
                                                                    gCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCa                               <  1:3054724/100‑1 (MQ=255)
                                                                     cACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCAt                              <  2:18881/100‑1 (MQ=255)
                                                                     cACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCAt                              >  2:3658230/1‑100 (MQ=255)
                                                                       cTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCt                            <  1:3031146/100‑1 (MQ=255)
                                                                        tttCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCtt                           <  1:2122988/100‑1 (MQ=255)
                                                                        tttCGTCAGTAAGCTGAATTTGCCTGGAACGTAGCCGGGCAATTTTTCTATTAACTCTCCGTTTCATGCTTAAACACAATTTCCTGTTTTTCTTCATCt                            <  2:3362696/99‑1 (MQ=255)
                                                                         ttCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGTGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTc                          >  2:1755709/1‑100 (MQ=255)
                                                                           cGTCAGTAATCTGAATTTGCCCGGCAAGTAGCCGGGAAATTTTGCTATTAACTCGCCGTTTCGGGCGTAAACAAAATATCCTGTTGTTATTCTTCTTACt                        >  2:3648514/1‑100 (MQ=255)
                                                                            gTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTc                       >  2:604545/1‑100 (MQ=255)
                                                                            gTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTc                       <  1:231560/100‑1 (MQ=255)
                                                                            gTCAGTAATCTGAATAAGCCCGGCACGTAGCCGTACAGTGTTGCAATTAACTCGCCCTTTCACGCTTAAACACCAGTTCCTTTTATTCTTCATATTCCt                        >  2:1590793/1‑99 (MQ=255)
                                                                               aGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcgac                    <  1:2390859/100‑1 (MQ=255)
                                                                                gTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcgacg                   >  2:2323112/1‑100 (MQ=255)
                                                                                gTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcgacg                   <  2:1816554/100‑1 (MQ=255)
                                                                                   aTCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACt                >  1:231846/1‑100 (MQ=255)
                                                                                    tCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTg               >  1:876344/1‑100 (MQ=255)
                                                                                     gcaaaattGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTgg              >  1:152639/7‑100 (MQ=38)
                                                                                     gcaaaattGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTgg              >  1:3619189/7‑100 (MQ=38)
                                                                                      tGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGATGACTGgc             <  2:2969369/100‑1 (MQ=255)
                                                                                      tGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGgc             >  2:1371376/1‑100 (MQ=255)
                                                                                      tGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGgc             >  2:450037/1‑100 (MQ=255)
                                                                                       gAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGgcg            <  1:3049423/100‑1 (MQ=255)
                                                                                        aaattGCCCGGCTACGTGCCGGGCAAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGgcg            >  2:3732738/4‑100 (MQ=37)
                                                                                        aaTTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAAATCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGgcgc           <  1:1032857/100‑1 (MQ=255)
                                                                                         aattGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCa          >  1:3774744/3‑100 (MQ=255)
                                                                                         aTTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCa          >  1:2000095/1‑100 (MQ=255)
                                                                                          tttGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAg         <  2:420406/100‑1 (MQ=255)
                                                                                            tGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCCGTg       >  2:744487/1‑100 (MQ=255)
                                                                                            tGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAg         >  2:870612/1‑98 (MQ=255)
                                                                                            tGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTg       >  2:3662378/1‑100 (MQ=255)
                                                                                            tGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTg       >  1:1820508/1‑100 (MQ=255)
                                                                                            tGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTg       >  2:1010774/1‑100 (MQ=255)
                                                                                             gCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGc      <  2:679043/100‑1 (MQ=255)
                                                                                              cccGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGc      >  2:2432337/1‑99 (MQ=255)
                                                                                              cccGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCt     >  2:517853/1‑100 (MQ=255)
                                                                                              cccGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCt     >  1:2228664/1‑100 (MQ=255)
                                                                                              cccGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCt     >  2:1532790/1‑100 (MQ=255)
                                                                                                cGGCACGTAGCCGTGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGTCTGGCGCAGTGCtgt   <  2:681497/100‑1 (MQ=255)
                                                                                                 ggCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCtgtg  >  1:1464733/1‑100 (MQ=255)
                                                                                                    |                                                                                                  
TGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTG  >  NC_000913/1524359‑1524555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: