BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,182,806	C→A	16.8%	P86Q (CCA→CAA)	zupT →	zinc transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,182,806	0	C	A	16.8%	352.9 / 60.9	137	P86Q (CCA→CAA)	zupT	zinc transporter
Reads supporting (aligned to +/- strand): ref base C (61/53); new base A (12/11); total (73/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.83e-01

Only 100 of 151 total aligned reads displayed.

TTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCGGCATCAGTCTGCA  >  NC_000913/3182707‑3182905

ttCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGcc                                                                                                     <  1:53139/100‑1 (MQ=255)

ttCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGcc                                                                                                     <  2:3207312/100‑1 (MQ=255)

ttCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGcc                                                                                                     <  1:3777511/100‑1 (MQ=255)

ttCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGcc                                                                                                     <  2:2027324/100‑1 (MQ=255)

 tCCTGCCGCACTAGCAGCTGAAGGAATGTCGGCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCca                                                                                                    <  2:2409078/100‑1 (MQ=255)

   cTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCcaca                                                                                                  >  1:1992995/1‑100 (MQ=255)

   cTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCcaca                                                                                                  <  1:2828375/100‑1 (MQ=255)

   cTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCcaca                                                                                                  >  1:3078394/1‑100 (MQ=255)

    tGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACAt                                                                                                 >  2:2869716/1‑100 (MQ=255)

     gCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATg                                                                                                >  1:320914/1‑100 (MQ=255)

     gCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTGGCAACATg                                                                                                <  2:1689770/100‑1 (MQ=255)

     gCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGGTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATg                                                                                                >  2:1416695/1‑100 (MQ=255)

        gtaCTAGCAGCTGAAGTAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTGTTTTGGCCTGGACCGCATGTTGCcaca                                                                                                  <  2:864309/93‑1 (MQ=255)

        ggaCTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTGGCAACATGcgn                                                                                             <  2:2153129/98‑3 (MQ=255)

        gCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTGGCAACATGcgg                                                                                             <  1:2618194/100‑3 (MQ=255)

         cACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTGGCAACATGcggt                                                                                            <  1:2032968/100‑4 (MQ=255)

         cACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTGGCAACATGcggt                                                                                            <  1:906488/100‑4 (MQ=255)

          aCTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTGGCAACATGcggt                                                                                            <  1:1005480/99‑4 (MQ=255)

           cTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTGGCAACATGcggtcc                                                                                          <  1:1417163/100‑6 (MQ=255)

             agcagcTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCg                                                                                        <  1:637269/100‑1 (MQ=255)

             agcagcTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTGGCAACATGcggtccag                                                                                        <  2:1024121/100‑8 (MQ=255)

             agcagcTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTGGCAACATGcggtccag                                                                                        <  1:1556831/100‑8 (MQ=255)

              gcagcTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGc                                                                                       >  2:1498327/1‑100 (MQ=255)

              gcagcTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGc                                                                                       >  1:3352952/1‑100 (MQ=255)

               cagcTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTGGCAACATGcggtccaggc                                                                                      <  1:2335879/100‑10 (MQ=255)

                agcTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCan                                                                                     <  2:2020665/100‑2 (MQ=255)

                 gcTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAgg                                                                                    >  2:3299083/1‑100 (MQ=255)

                 gcTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAgg                                                                                    <  1:2797210/100‑1 (MQ=255)

                  cTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGa                                                                                   >  1:3781160/1‑100 (MQ=255)

                  cTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGa                                                                                   <  1:3477886/100‑1 (MQ=255)

                     aaGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGAttt                                                                                <  1:2227028/100‑1 (MQ=255)

                     aaGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGAttt                                                                                <  1:1198635/100‑1 (MQ=255)

                       ggAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTaa                                                                              >  1:1060220/1‑100 (MQ=255)

                              cGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCaaaa                                                                       >  1:3416597/1‑100 (MQ=255)

                                ccTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAAt                                                                     >  1:1451391/1‑100 (MQ=255)

                                   gtgtTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCgg                                                                  >  1:497998/1‑100 (MQ=255)

                                    tgtTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGt                                                                 >  1:1473284/1‑100 (MQ=255)

                                    tgtTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGt                                                                 >  2:440378/1‑100 (MQ=255)

                                      ttGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGc                                                               <  2:2081769/100‑1 (MQ=255)

                                       tGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCa                                                              >  2:1853800/1‑100 (MQ=255)

                                         ggTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGc                                                            >  2:408444/1‑100 (MQ=255)

                                         ggTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGc                                                            <  2:3615875/100‑1 (MQ=255)

                                          gTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGcc                                                           >  1:2559325/1‑100 (MQ=255)

                                          gTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGcc                                                           >  2:2872742/1‑100 (MQ=255)

                                           ttATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCg                                                          <  2:2407799/100‑1 (MQ=255)

                                           ttATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCg                                                          <  1:2449554/100‑1 (MQ=255)

                                              tGGCATGGTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAGTGCCAAAATCGGTGCAGCCGt                                                         >  2:1870337/1‑98 (MQ=255)

                                               ggAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGc                                                      <  1:2154927/100‑1 (MQ=255)

                                               ggAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGc                                                      <  1:1672972/100‑1 (MQ=255)

                                               ggAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGc                                                      >  2:3050960/1‑100 (MQ=255)

                                                 aaTGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCa                                                    <  1:1835517/100‑1 (MQ=255)

                                                   tGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATGTAATGCAAAAATCGGTGCAGCCGTTGCCaaa                                                  >  2:3576283/1‑100 (MQ=255)

                                                      ttATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATc                                               <  1:3115884/100‑1 (MQ=255)

                                                       tATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGAAATAAATAAAAAAATCGGTGCAGCCGTTGCCAAAATCg                                              <  2:3261391/100‑1 (MQ=255)

                                                         tCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGa                                             >  2:1706678/1‑99 (MQ=255)

                                                          cTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATc                                           <  1:223154/100‑1 (MQ=255)

                                                          cTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATc                                           <  2:3777307/100‑1 (MQ=255)

                                                          cTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATc                                           >  1:1347894/1‑100 (MQ=255)

                                                          cTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATc                                           <  1:1971965/100‑1 (MQ=255)

                                                           ttCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGTCACATGCTCATCCGAAGAAAAAAAAAAAAAAATCGGTGCAGCCGTTGCCAAAATCGATc                                           <  2:1581267/99‑1 (MQ=255)

                                                            tCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCaa                                         >  2:360076/1‑100 (MQ=255)

                                                            tCGGTCTGCTTGGCTATTTCGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAAAAAATAAAAAAAAAATCGGTGCAGCCGTTGCCAAAATCGAt                                            <  2:1781898/97‑1 (MQ=255)

                                                             cGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAg                                        >  1:655364/1‑100 (MQ=255)

                                                              ggTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAgc                                       <  1:2196453/100‑1 (MQ=255)

                                                                 cTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAAAAAATAAAACAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGca                                    <  2:3352926/100‑1 (MQ=255)

                                                                  ttcTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCATAAATCGGTGCAGCCGTTGCCAAAAGCGATCAAGCGcac                                   <  2:2355184/98‑1 (MQ=255)

                                                                  tGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGcac                                   >  1:2473834/1‑100 (MQ=255)

                                                                    cTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCAGTTGCCAAAATCGATCAAGCGCACAg                                 >  2:3218836/1‑100 (MQ=255)

                                                                      tGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGcc                               >  2:1711868/1‑100 (MQ=255)

                                                                         cTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCAtt                            <  2:3650325/100‑1 (MQ=255)

                                                                           aTTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCt                          <  1:2062047/100‑1 (MQ=255)

                                                                            ttttGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTg                         <  1:2436385/100‑1 (MQ=255)

                                                                            ttttGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTg                         <  1:1544551/100‑1 (MQ=255)

                                                                             tttGGCCTGGATCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGc                        <  2:2562873/100‑1 (MQ=255)

                                                                             tttGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGc                        >  1:1494356/1‑100 (MQ=255)

                                                                              ttGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCt                       <  1:1773260/100‑1 (MQ=255)

                                                                              ttGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTCCCAAAATCGATCAAGCGGACAGCCATTCttc                        <  1:2573513/99‑3 (MQ=255)

                                                                               tGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTc                      <  1:2869736/100‑1 (MQ=255)

                                                                                ggCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCa                     >  1:1723435/1‑100 (MQ=255)

                                                                                ctgcggatgagCATGTGGCAACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCa                     >  1:163064/11‑100 (MQ=38)

                                                                                 gCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCAc                    <  1:2896649/100‑1 (MQ=255)

                                                                                  ccTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCAct                   >  1:3122926/1‑100 (MQ=255)

                                                                                    ggatgagCATGTGGCAACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGCTCAAGCGCACAGCCATTCTGCTCActct                 >  2:658067/7‑100 (MQ=255)

                                                                                    gaaggagCATG‑TGCAACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGAGCACAGCCATTCTGCTCACtctc                >  2:2711971/7‑100 (MQ=255)

                                                                                     ggACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACtctc                <  1:1169547/100‑1 (MQ=255)

                                                                                     gatgagCATGTGGCAACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCActccc                >  1:993633/6‑98 (MQ=255)

                                                                                     gatgagCATGTGGCAACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACtctc                >  2:1268869/6‑100 (MQ=255)

                                                                                     gatgagCATGTGGCAACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACtctc                >  2:1215473/6‑100 (MQ=255)

                                                                                     gatgagCATGTGGCAACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACtctc                >  2:3374840/6‑100 (MQ=255)

                                                                                     gatgagCATGTGGCAACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACtctc                >  1:668554/6‑100 (MQ=255)

                                                                                       tgagCATGTGGCAACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAACATCGATCAAGCGCACAGCCATTCTGCTCACTCTCgg              >  2:63582/4‑100 (MQ=255)

                                                                                        gagCATGTGGCAACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCGGc             >  2:1171350/3‑100 (MQ=255)

                                                                                        ccGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCGGc             >  2:2185625/1‑100 (MQ=255)

                                                                                        ccGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCGGc             <  2:2765754/100‑1 (MQ=255)

                                                                                          gCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCGGCAt           >  1:876187/1‑100 (MQ=255)

                                                                                             tGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCGGCATCAg        >  2:1428915/1‑100 (MQ=255)

                                                                                              gTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCGGCATCAGt       >  1:3290258/1‑100 (MQ=255)

                                                                                               ttGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCGGCATCAGTc      >  2:1248435/1‑100 (MQ=255)

                                                                                                 gCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAGAATCGATCACGCGCAGAGCCATTCGGCTCACTCTCGGCATCAGTCTg    >  2:2579325/1‑100 (MQ=255)

                                                                                                  ccACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCGGCATCAGTCTGc   <  1:2547893/100‑1 (MQ=255)

TTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCGGCATCAGTCTGCA  >  NC_000913/3182707‑3182905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`