Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,471,361 T→G 15.2% intergenic (‑32/+39) tufA ← / ← fusA translation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,471,3610TG15.2% 533.6 / 83.1 214intergenic (‑32/+39)tufA/fusAtranslation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding
Reads supporting (aligned to +/- strand):  ref base T (93/88);  new base G (19/14);  total (112/102)
Fisher's exact test for biased strand distribution p-value = 5.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

Only 100 of 216 total aligned reads displayed.

TGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTC  >  NC_000913/3471262‑3471460
                                                                                                   |                                                                                                   
tGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTATCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCtt                                                                                                     >  2:2438103/1‑100 (MQ=255)
  gTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACTGCTTTTTTTTTTATTATATTGCTCTCCTCTTCa                                                                                                   >  2:156870/1‑100 (MQ=17)
  gTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCa                                                                                                   >  2:1410182/1‑100 (MQ=255)
   tCAACGTGGCCGTGAGTTCCTACGTCAACGGGTGGTTTTGTACTTGCAAATTTTCCTTTAGACACGGCTATATTCCGTACTATAGTGCTCTCCCCTTCat                                                                                                  <  2:759672/100‑2 (MQ=255)
   tCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAg                                                                                                  >  1:286463/1‑100 (MQ=255)
   tCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAg                                                                                                  <  2:1852829/100‑1 (MQ=255)
    cAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAgg                                                                                                 <  2:85757/100‑1 (MQ=255)
        gTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagag                                                                                             <  2:468765/100‑1 (MQ=255)
         tGGCCGATAGTACCAATGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTAAGACACGGCGATATTACTTACTATGGTGCTCTCCCCTTCAGgagaga                                                                                            >  2:2577542/1‑100 (MQ=25)
          ggCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGagagag                                                                                           >  1:2094727/1‑100 (MQ=255)
           gCCGATAGTACCAACGTTAACGTGCGTTTTTGTACGTTCAAATTTTTCTATAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGggagag                                                                                           <  1:921702/99‑1 (MQ=25)
           gCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGc                                                                                          <  2:305768/100‑1 (MQ=21)
           gCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGc                                                                                          >  2:2283563/1‑100 (MQ=255)
            ccGATAGTGCCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGGCACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGc                                                                                          <  1:322983/99‑1 (MQ=25)
             cGATAGTACTTACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTTTAGTGCTCTCTCCTTGAGGAGAGAGCAc                                                                                        >  2:3208786/1‑100 (MQ=25)
             cGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCAc                                                                                        <  1:2163343/100‑1 (MQ=25)
              gATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACg                                                                                       <  1:2386264/100‑1 (MQ=255)
               aTAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACgg                                                                                      <  1:385681/100‑1 (MQ=255)
                tAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCAc                                                                                        <  2:1143272/97‑1 (MQ=25)
                  gTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTGCAGGAGAGAGCACGGGAc                                                                                   <  2:407395/100‑1 (MQ=255)
                    aCCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACtt                                                                                 >  1:2147635/1‑100 (MQ=255)
                     ccAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACttt                                                                                >  1:3431196/1‑100 (MQ=255)
                     ccAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACttt                                                                                >  2:578064/1‑100 (MQ=255)
                     ccAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACttt                                                                                >  2:377963/1‑100 (MQ=255)
                      cAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTAATATAGTGCTCTCCCCTTCAGGAGAGAGCagatcggaaga                                                                               >  1:1069815/1‑90 (MQ=37)
                       aaCGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTgg                                                                              <  1:1279106/100‑1 (MQ=255)
                        aCGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTTCATGAGAGAGCACGGGACTTTgg                                                                              <  2:2792750/99‑1 (MQ=255)
                         cGTTAACGTGCGGTTTTGTGCGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTa                                                                            <  2:1448054/100‑1 (MQ=255)
                           ttAACGTGCGGTTGTGTACGTTCAAATTTTGCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTAt                                                                           <  2:3158137/99‑1 (MQ=255)
                            tAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTa                                                                         <  1:607815/100‑1 (MQ=255)
                            tAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTa                                                                         <  1:1944800/100‑1 (MQ=255)
                                gTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAccc                                                                     >  1:2044560/1‑100 (MQ=255)
                                 tGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCt                                                                    >  1:3689790/1‑100 (MQ=255)
                                   cGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTa                                                                  <  1:2749128/100‑1 (MQ=255)
                                   cGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTa                                                                  >  1:2626003/1‑100 (MQ=255)
                                   cGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTa                                                                  >  1:251433/1‑100 (MQ=255)
                                    ggTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCGTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAg                                                                 >  2:2196904/1‑100 (MQ=255)
                                     gTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAgg                                                                <  1:354018/100‑1 (MQ=255)
                                     gTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAgg                                                                <  2:2894638/100‑1 (MQ=255)
                                       tttGTTCGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCt                                                              <  2:3746339/100‑1 (MQ=255)
                                       ccgatctgTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCt                                                              <  1:2909751/93‑1 (MQ=255)
                                         tGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTa                                                            >  1:1151333/1‑100 (MQ=255)
                                          gTACGTTCACATTTTTCTTTAGACACGGCAATATACCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTACCCCTTAGGCTTa                                                            >  2:613834/1‑99 (MQ=255)
                                          gTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTAt                                                           >  2:2160604/1‑100 (MQ=255)
                                           tACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTTCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTAtt                                                          >  2:348800/1‑100 (MQ=255)
                                              gTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGGTTATTTAc                                                       >  2:1236008/1‑100 (MQ=255)
                                              gTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAc                                                       >  1:3356029/1‑100 (MQ=255)
                                              gTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAc                                                       >  2:2996613/1‑100 (MQ=255)
                                              gTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAc                                                       <  1:19042/100‑1 (MQ=255)
                                                taacaTTTTTCTTTAGAGGGGGCTCTATTCCTTACTATTGTCCTCTGCACTTCGGGCGAGGGCTCGGGAATTTGGTATTAACCCTTAGGCTTGGTTAct                                                      >  2:2717707/5‑98 (MQ=255)
                                                  aaaTTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACg                                                   >  2:2463924/1‑100 (MQ=255)
                                                          cTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCaa                                           <  2:3277076/100‑1 (MQ=255)
                                                          cTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCaa                                           >  2:1963573/1‑100 (MQ=255)
                                                          cTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCaa                                           >  2:1346712/1‑100 (MQ=255)
                                                               gACACGGCTATATTCCTTACTATAGTGCTCTTCCCTTCAGGAGATAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTa                                        <  2:1063730/98‑1 (MQ=255)
                                                                acacGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGTCTTCAATTACgg                                     <  1:2509817/100‑1 (MQ=255)
                                                                acacGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACgg                                     <  1:2809879/100‑1 (MQ=255)
                                                                 cacGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAGTTACGGc                                    >  2:2051338/1‑100 (MQ=255)
                                                                  acGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGcc                                   <  1:3798612/100‑1 (MQ=255)
                                                                   cGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCt                                  >  2:2667448/1‑100 (MQ=255)
                                                                   cGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCt                                  >  1:641162/1‑100 (MQ=255)
                                                                   cGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCt                                  >  2:2482718/1‑100 (MQ=255)
                                                                    ggCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTg                                 <  1:2283577/100‑1 (MQ=255)
                                                                    ggCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTg                                 <  1:553624/100‑1 (MQ=255)
                                                                     gCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGa                                <  2:1171442/100‑1 (MQ=255)
                                                                     gCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGa                                <  2:2394573/100‑1 (MQ=255)
                                                                      cTATATTCCTTACTATATTGCTCTCCCCTTCAGGAGAGAGCACGGGACCTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                               <  1:1561786/100‑1 (MQ=255)
                                                                      cTATATTCCTTACTATAGTGTTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                               <  2:459823/100‑1 (MQ=255)
                                                                       tataTTCCTTACTATAGTGTTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGc                              <  1:2557247/100‑1 (MQ=255)
                                                                       tataTTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGc                              <  1:1392777/100‑1 (MQ=255)
                                                                          atTCCTTACTATAGTGCTCTCCCCTTCAGGATAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAAc                           >  2:84072/1‑100 (MQ=255)
                                                                           ttCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACg                          >  1:702264/1‑100 (MQ=255)
                                                                           ttCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACg                          <  1:2014263/100‑1 (MQ=255)
                                                                               ttACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTAc                      <  1:1615363/100‑1 (MQ=255)
                                                                                 aCTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTc                    <  2:3402093/100‑1 (MQ=255)
                                                                                  cTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCg                   >  2:718840/1‑100 (MQ=255)
                                                                                  cTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCg                   >  2:2920749/1‑100 (MQ=255)
                                                                                   tataGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCgg                  >  1:1126715/1‑100 (MQ=255)
                                                                                    cccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgc                 >  1:3030726/4‑100 (MQ=38)
                                                                                     ccgTGCTCTCTCCTGAAGGGGAGCGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATCACGGCCTGAGCAACGTTACTCg                   >  2:3179333/3‑97 (MQ=37)
                                                                                      cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcgc               >  1:2116621/2‑100 (MQ=38)
                                                                                      aGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcgc               <  2:3223403/100‑1 (MQ=255)
                                                                                      aGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcgc               >  2:645859/1‑100 (MQ=255)
                                                                                       gTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCt              <  2:180158/100‑1 (MQ=255)
                                                                                        tGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCtt             >  1:1438664/1‑100 (MQ=255)
                                                                                        tGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCtt             >  1:2886064/1‑100 (MQ=255)
                                                                                         gCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtc            >  2:3122085/1‑100 (MQ=255)
                                                                                         gCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTCCCACGGGCTTCAATTACGGCCTGAGCAACATCACCcgccgca              <  2:3580360/98‑2 (MQ=255)
                                                                                          ctctctCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtc            >  1:1590089/1‑99 (MQ=255)
                                                                                           tctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcat          >  2:3042333/1‑100 (MQ=255)
                                                                                            ctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc         >  1:1875137/1‑100 (MQ=255)
                                                                                            ctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc         >  1:1195985/1‑100 (MQ=255)
                                                                                            ctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc         <  2:2851143/100‑1 (MQ=255)
                                                                                             tcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatca        <  1:2422930/100‑1 (MQ=255)
                                                                                             tcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatca        <  1:2989297/100‑1 (MQ=255)
                                                                                                ccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCGTAc     >  2:3617250/1‑100 (MQ=255)
                                                                                                ccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATAc     <  1:2470531/100‑1 (MQ=255)
                                                                                                ccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATAc     <  1:3242671/100‑1 (MQ=255)
                                                                                                ccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATAc     >  1:2878342/1‑100 (MQ=255)
                                                                                                   tCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTc  <  1:358039/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
TGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTC  >  NC_000913/3471262‑3471460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: