Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,476,585 G→A 20.5% intergenic (‑145/+22) yheO ← / ← fkpA putative PAS domain‑containing DNA‑binding transcriptional regulator/FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,476,5850GA20.5% 380.1 / 83.4 181intergenic (‑145/+22)yheO/fkpAputative PAS domain‑containing DNA‑binding transcriptional regulator/FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)
Reads supporting (aligned to +/- strand):  ref base G (75/68);  new base A (16/21);  total (92/89)
Fisher's exact test for biased strand distribution p-value = 3.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.11e-01

Only 100 of 190 total aligned reads displayed.

GCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAG  >  NC_000913/3476486‑3476684
                                                                                                   |                                                                                                   
 cAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGt                                                                                                    <  2:1068902/100‑1 (MQ=255)
   ggCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCa                                                                                                  >  1:2892975/1‑100 (MQ=255)
    gCTCATTAATGATGTGGGTAACTGACTTAATACAGCGGAGGTTCCGCTTTCTAGCACTTATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTc                                                                                                   <  2:3593043/98‑1 (MQ=255)
     cTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAgg                                                                                                >  1:1001724/1‑100 (MQ=255)
      tCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAggc                                                                                               <  1:2804355/100‑1 (MQ=255)
      tCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCAGCTTTCCAGGACTAATTATATCCGGCACGTAATAAAAAAACCGCCGCCTGGTCAggc                                                                                               >  2:3083156/1‑100 (MQ=255)
          tAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAACACCGCCGCCTGACCAGgcggcg                                                                                           <  2:2211703/100‑1 (MQ=255)
           aaTGATGCGGGTAACTAAATTAATACAGCGGAGTTTCCGCTTTCCAGCACTAATTATATCCGGAAAAAAAAAAAAAAACCGCCGCCTGACCAGGcggcgg                                                                                          <  2:1553124/100‑1 (MQ=255)
           aaTGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGACCAGGcggcgg                                                                                          <  1:1022680/100‑1 (MQ=255)
           aaTGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGACCAGGcggcgg                                                                                          <  1:1232123/100‑1 (MQ=255)
           aaTGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGACCAGGcggcgg                                                                                          <  1:780732/100‑1 (MQ=255)
           aaTGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGACCAGGcggcgg                                                                                          <  1:2271655/100‑1 (MQ=255)
            atgatgCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGACCAGGCGGCGGt                                                                                         <  2:134310/100‑1 (MQ=255)
            atgatgCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGACCAGGCGGCGGt                                                                                         <  1:2433452/100‑1 (MQ=255)
            atgatgCGGGTAACTAAATAAATACAGCGGACGTTCCGCTTTCCCGCTCTAACTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGACCAGGCGGCGGt                                                                                         <  2:2582750/100‑1 (MQ=255)
               atgCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCt                                                                                      <  1:339013/100‑1 (MQ=255)
                tgCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCtt                                                                                     <  1:2714312/100‑1 (MQ=255)
                 gCGGGTAACAAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGACTGTAAAAAAAAAACCGCCGCCTGACCAGGCGGCGGtt                                                                                        <  2:432206/96‑1 (MQ=38)
                  cGGGTAACTAAATTATTACAGCGGAGGTTCCGCTTTCCAGCACTAGTTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGACCAGGCGGCGGTtttttt                                                                                   <  1:3756377/100‑3 (MQ=37)
                  cGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTaa                                                                                   >  2:126049/1‑100 (MQ=255)
                  cGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTaa                                                                                   >  1:2561045/1‑100 (MQ=255)
                    ggTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATg                                                                                 >  2:3724576/1‑100 (MQ=255)
                      tAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCt                                                                               <  1:1837939/100‑1 (MQ=255)
                        aCTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTa                                                                             >  1:3696983/1‑100 (MQ=255)
                        aCTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTa                                                                             <  2:3293627/100‑1 (MQ=255)
                          tAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTAtt                                                                           >  1:3005929/1‑100 (MQ=255)
                          tAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTAtt                                                                           >  2:3394780/1‑100 (MQ=255)
                           aaaTTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGATCTTAATGCTTAttt                                                                          >  1:1460450/1‑100 (MQ=255)
                            aaTTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTAtttt                                                                         >  1:1537696/1‑100 (MQ=255)
                            aaTTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTAtttt                                                                         >  1:448035/1‑100 (MQ=255)
                            aaTTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTAtttt                                                                         <  2:2343474/100‑1 (MQ=255)
                              ttAATACAGCGGAGTTTCCGCTTTCCAGCACTAATTATATCCTGCCTGTAATATAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTAtgtttt                                                                       <  2:764988/100‑1 (MQ=255)
                               tAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTa                                                                      <  2:1013322/100‑1 (MQ=255)
                                aaTACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAg                                                                     <  2:279592/100‑1 (MQ=255)
                                aaTACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAg                                                                     >  1:3512060/1‑100 (MQ=255)
                                 aTACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGc                                                                    >  1:1047985/1‑100 (MQ=255)
                                  tACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGc                                                                    <  2:1990375/99‑1 (MQ=255)
                                  tACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCa                                                                   >  1:394899/1‑100 (MQ=255)
                                    cAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGa                                                                 >  1:865037/1‑100 (MQ=255)
                                      gCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAAt                                                               >  1:2641208/1‑100 (MQ=255)
                                      gCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAAt                                                               >  2:2154876/1‑100 (MQ=255)
                                         gAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTg                                                            <  2:2540393/100‑1 (MQ=255)
                                           ggTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCg                                                          <  2:2399013/100‑1 (MQ=255)
                                           ggTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCg                                                          <  1:176682/100‑1 (MQ=255)
                                           ggTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCg                                                          >  1:1659848/1‑100 (MQ=255)
                                            gTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCgg                                                         >  1:984480/1‑100 (MQ=255)
                                            gTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGc                                                        <  1:1603519/100‑1 (MQ=255)
                                               ccGCTGTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGc                                                        <  1:3577637/98‑1 (MQ=255)
                                                 gCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTc                                                    >  2:2866639/1‑100 (MQ=255)
                                                    ttCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCa                                                 <  1:1741000/100‑1 (MQ=255)
                                                        aGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAg                                             >  1:2392845/1‑100 (MQ=255)
                                                        aGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAg                                             <  2:2491536/100‑1 (MQ=255)
                                                            cTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCATCTTc                                         >  2:3109963/1‑100 (MQ=255)
                                                             tAATTATATCCGGCCTGTAATAAAAAACCCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTcc                                        <  2:664045/100‑1 (MQ=255)
                                                               aTTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCgg                                      >  1:1929504/1‑100 (MQ=255)
                                                                   tatCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCttt                                  >  2:3798279/1‑100 (MQ=255)
                                                                    atCCTGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTg                                 <  2:127698/100‑1 (MQ=255)
                                                                    atCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTg                                 <  2:3317879/100‑1 (MQ=255)
                                                                      ccGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCa                               <  1:2970574/100‑1 (MQ=255)
                                                                        ggCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTTTTTGTATTTGCGGCTTTCGCATCAGCTTCCGGCTTTGCa                               >  2:2022093/1‑98 (MQ=255)
                                                                            tGTAATAATAAAACCGCTGCCTTGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCa                               <  2:3516911/94‑1 (MQ=255)
                                                                            tGTAATAAAAAAACCGCCGCCTGGTCAGGCGGTGGTTCTCAACGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGcc                         <  2:2680482/100‑1 (MQ=255)
                                                                              taataaAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCtt                       <  1:2866659/100‑1 (MQ=255)
                                                                               gcattaagaaCCGCCGCCTGACCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTc                      >  1:2040236/9‑100 (MQ=37)
                                                                               aataaAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCTGCTTTGCATCAGCCTTc                      >  2:3037228/1‑100 (MQ=255)
                                                                               aataaAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTc                      >  2:1902572/1‑100 (MQ=255)
                                                                                cattaagaaCCGCCGCCTGACCAGGCGGCGGTTTTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCg                     >  1:1301664/8‑100 (MQ=37)
                                                                                cattaagaaCCGCCGCCTGACCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCg                     >  2:324268/8‑100 (MQ=38)
                                                                                ataaAAAAACCGCCGCCTGGTCAGGCTGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCg                     <  2:1751973/100‑1 (MQ=255)
                                                                                ataaAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCg                     >  1:3226175/1‑100 (MQ=255)
                                                                                 taaAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCgg                    <  2:3017900/100‑1 (MQ=255)
                                                                                 taaAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTCCATCAGCCTTccg                    <  1:2593402/100‑3 (MQ=255)
                                                                                  aaaaaaaCCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGgc                   >  2:1027095/1‑100 (MQ=255)
                                                                                  aaaaaaaCCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGgc                   <  1:2135173/100‑1 (MQ=255)
                                                                                    aaaaaCCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGgcgc                 >  2:3494209/1‑100 (MQ=255)
                                                                                     agaaCCGCCGCCTGACCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGGATCTGCGGCTTTCGCATTAGCTTCTGGCTTTGCATCAGCCTTCGGCGCt                >  2:3077624/3‑100 (MQ=38)
                                                                                        aCCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGt             >  2:2755350/1‑100 (MQ=255)
                                                                                        aCCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGt             >  2:354394/1‑100 (MQ=255)
                                                                                        aCCGCCGCCTGACCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCTGCGCTCGt             >  2:792115/1‑100 (MQ=255)
                                                                                        aCCGCCGCCTGACCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGt             >  1:922347/1‑100 (MQ=255)
                                                                                         ccgccgCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGtt            <  2:207870/100‑1 (MQ=255)
                                                                                         ccgccgCCTGACCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGtt            >  1:1248604/1‑100 (MQ=255)
                                                                                         ccgccgCCTGACCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGtt            >  2:544399/1‑100 (MQ=255)
                                                                                          cgccgcCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATGAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGttt           >  1:2685006/1‑100 (MQ=255)
                                                                                          cgccgcCTGACCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGttt           >  1:2906935/1‑100 (MQ=255)
                                                                                          cgccgcCTGACCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCCGGTTTGCATAAGCCTTCGGCGCTGGttt           >  2:3388488/1‑100 (MQ=255)
                                                                                           gccgccTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTc          >  1:2325434/1‑100 (MQ=255)
                                                                                           gccgccTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTc          <  2:1838604/100‑1 (MQ=255)
                                                                                            ccgccTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTca         <  2:2287589/100‑1 (MQ=255)
                                                                                            ccgccTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTca         <  1:216904/100‑1 (MQ=255)
                                                                                             cgccTGGTCCGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTca         <  2:2834137/99‑1 (MQ=255)
                                                                                             cgccTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTcac        <  2:1684364/100‑1 (MQ=255)
                                                                                              gccTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTcaca       >  2:1610742/1‑100 (MQ=255)
                                                                                              gccTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTcaca       <  2:786611/100‑1 (MQ=255)
                                                                                               ccTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTggatgtgaaa      <  1:3320015/100‑9 (MQ=255)
                                                                                               ccTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACAt      >  1:3740120/1‑100 (MQ=255)
                                                                                                  ggTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCca   <  1:198186/100‑1 (MQ=255)
                                                                                                  ggTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCca   <  2:1764867/100‑1 (MQ=255)
                                                                                                   gTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCcag  >  1:759408/1‑100 (MQ=255)
                                                                                                   gTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCcag  <  1:1870449/100‑1 (MQ=255)
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GCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAG  >  NC_000913/3476486‑3476684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: