Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,705,971 C→A 24.8% intergenic (‑181/+127) dppB ← / ← dppA dipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9710CA24.8% 582.5 / 185.5 247intergenic (‑181/+127)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base C (85/100);  new base A (54/7);  total (139/108)
Fisher's exact test for biased strand distribution p-value = 2.02e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-02

Only 100 of 256 total aligned reads displayed.

AGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCC  >  NC_000913/3705872‑3706069
                                                                                                   |                                                                                                     
aGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgc                                                                                                       <  1:2975533/100‑1 (MQ=255)
      tGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGtt                                                                                                 <  1:1181234/100‑1 (MQ=255)
      tGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGtt                                                                                                 <  2:1922835/100‑1 (MQ=255)
      tGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGtt                                                                                                 <  2:3658811/100‑1 (MQ=255)
      tGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGtt                                                                                                 <  2:3080685/100‑1 (MQ=255)
       gAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTa                                                                                                <  1:3152769/100‑1 (MQ=255)
       gAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTa                                                                                                <  2:479171/100‑1 (MQ=255)
       gAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTa                                                                                                <  1:1341473/100‑1 (MQ=255)
            aTTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGcc                                                                                           >  2:238748/1‑100 (MQ=255)
             ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGGTTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                                          >  2:1574190/1‑100 (MQ=255)
             ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGACt                                                                                          <  1:221453/100‑1 (MQ=255)
              tgctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCtt                                                                                         >  2:3728242/1‑100 (MQ=255)
               gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                        >  1:1757085/1‑100 (MQ=255)
                ctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTGTACAGATTGTTGAGAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTAt                                                                                       >  2:1275967/1‑100 (MQ=255)
                  gcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCtagatc                                                                                     >  2:1367714/1‑95 (MQ=255)
                  gcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATcc                                                                                     >  1:3082497/1‑100 (MQ=255)
                    cGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACATAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCgg                                                                                   >  2:1210838/1‑100 (MQ=255)
                    cGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGTTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCgg                                                                                   <  1:796663/100‑1 (MQ=37)
                    cGGATGGCGCTGCACGCAGAGGCACAGAGCCACCGGGTACACAGATTGTTGACAAAGTGCACTTTGTTTATTCCGAATGCGGCGTTAACGCCTTATCCg                                                                                    >  2:3456794/1‑99 (MQ=25)
                     ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                                  <  2:2292419/100‑1 (MQ=255)
                      gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                 <  2:3691514/100‑1 (MQ=255)
                      gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                 <  1:3307969/100‑1 (MQ=255)
                      gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                 <  2:3503374/100‑1 (MQ=255)
                       aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCt                                                                                <  1:2151811/100‑1 (MQ=255)
                         ggCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAc                                                                              >  2:2988176/1‑100 (MQ=255)
                           cgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaa                                                                            >  1:2093381/1‑100 (MQ=255)
                           cgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGcataaac                                                                            <  1:3450998/100‑7 (MQ=25)
                           cgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCGTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGCATaaac                                                                            <  1:2034563/100‑4 (MQ=25)
                           cgcTGCACCCAGAGGCACAGCGCTACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCAGCCGGCATaaa                                                                             <  2:3592721/99‑3 (MQ=21)
                             cTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaaa                                                                          <  2:5648/100‑1 (MQ=255)
                               gCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATc                                                                        >  1:1083245/1‑100 (MQ=255)
                                cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCg                                                                       >  1:24115/1‑100 (MQ=255)
                                 aCGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGt                                                                      <  2:430322/100‑1 (MQ=255)
                                  cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                     >  2:967951/1‑100 (MQ=255)
                                  cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                     <  1:3817292/100‑1 (MQ=255)
                                   gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGTCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                    <  2:1606255/100‑1 (MQ=255)
                                          cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                             <  2:2240920/100‑1 (MQ=255)
                                          cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                             >  2:319885/1‑100 (MQ=255)
                                          cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                             >  1:1099019/1‑100 (MQ=255)
                                            caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                           <  1:2510151/100‑1 (MQ=255)
                                                gcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAt                                                       >  1:448764/1‑100 (MQ=255)
                                                 ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                                                      >  1:1150963/1‑100 (MQ=255)
                                                  cACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTg                                                     <  1:1439973/100‑1 (MQ=255)
                                                   aCCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGc                                                    <  2:2117254/100‑1 (MQ=255)
                                                     cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAg                                                 <  1:1955712/100‑1 (MQ=255)
                                                        gTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAt                                              <  2:1447367/100‑1 (MQ=255)
                                                          ataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAttt                                            >  2:2846995/1‑100 (MQ=255)
                                                          ataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAttt                                            >  2:3339301/1‑100 (MQ=255)
                                                          ataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAttt                                            <  2:2497902/100‑1 (MQ=255)
                                                           taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAtttt                                           <  2:3288902/100‑1 (MQ=255)
                                                           taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAtttt                                           <  2:1119414/100‑1 (MQ=255)
                                                            aCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTc                                          <  1:975882/100‑1 (MQ=255)
                                                             cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGTGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑g                                       <  2:2583548/100‑1 (MQ=255)
                                                             cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTTCAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑g                                       <  2:2038941/100‑1 (MQ=255)
                                                                  cataaaCAAAGCGCACTTTGTTTATGCCGGATGCGGCGTTAACACCTTATCCGGCCTACAAAATCGTGCAACTTCAATACATTGCAGAATTTTC‑‑GTAGGc                                  >  2:342775/6‑100 (MQ=17)
                                                                   gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGcc                                 <  1:317924/100‑1 (MQ=255)
                                                                   gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGcc                                 >  1:912497/1‑100 (MQ=255)
                                                                   ataaaCAAAGCGCACTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGcc                                 >  2:2252534/5‑100 (MQ=18)
                                                                    ttgACAAAGTGCGCTTTGTTTATGCTGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCt                                <  2:1203952/100‑1 (MQ=255)
                                                                      gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGa                              >  1:2389729/1‑100 (MQ=255)
                                                                       aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGAt                             >  1:1678092/1‑100 (MQ=255)
                                                                        cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATa                            <  2:1150954/100‑1 (MQ=255)
                                                                           aGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGc                         >  1:656338/1‑100 (MQ=255)
                                                                              gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGa                      <  2:1654144/100‑1 (MQ=255)
                                                                               cgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAg                     <  1:155417/100‑1 (MQ=255)
                                                                               cgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAg                     <  1:3339326/100‑1 (MQ=255)
                                                                                gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                    <  1:3377198/100‑1 (MQ=255)
                                                                                gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                    <  1:2677106/100‑1 (MQ=255)
                                                                                gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                    >  1:1466197/1‑100 (MQ=255)
                                                                                gcTTTGTTTATGCCGGATGCGGCATTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                    <  2:1036240/100‑1 (MQ=255)
                                                                                 cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAGTTTTC‑‑GTAGGCCTGATAAGCGGAgcg                   <  2:3784228/100‑1 (MQ=255)
                                                                                 cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgcg                   >  1:61407/1‑100 (MQ=255)
                                                                                 cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAACCGGAgcg                   >  2:593317/1‑100 (MQ=255)
                                                                                  tttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgcgc                  <  1:734008/100‑1 (MQ=255)
                                                                                  tttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgcg                   >  1:3771752/1‑99 (MQ=255)
                                                                                   ttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCa                 <  1:3634411/100‑1 (MQ=255)
                                                                                    cttgtaggCCGGATAAGGCGGTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCATGAGCCAC‑‑GTAGGCCTGATAAGCGTAGCGCAt                >  2:2330667/8‑100 (MQ=17)
                                                                                      tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCa              >  1:1193033/1‑100 (MQ=255)
                                                                                       ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAg             <  1:1316302/100‑1 (MQ=255)
                                                                                       gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTAT‑‑GTAGGCCTGATAAGCATAGCGCATCAg             >  1:1888601/5‑100 (MQ=18)
                                                                                        taggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTTCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg            >  1:2620268/4‑100 (MQ=17)
                                                                                        taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATCCTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg            >  2:2672484/4‑100 (MQ=18)
                                                                                         aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc           >  1:3671254/3‑100 (MQ=18)
                                                                                         aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc           >  1:3089403/3‑100 (MQ=18)
                                                                                         aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGCAATCTCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGc           >  2:1840792/3‑100 (MQ=18)
                                                                                          tGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCa          >  1:987211/1‑100 (MQ=255)
                                                                                          ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa          >  2:1754743/2‑100 (MQ=17)
                                                                                          ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCa          >  1:1846779/2‑100 (MQ=25)
                                                                                          ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa          >  2:2455217/2‑100 (MQ=17)
                                                                                          ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAGTTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAt         >  1:3785975/2‑100 (MQ=21)
                                                                                           gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt         >  2:2826802/1‑100 (MQ=255)
                                                                                           gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGcgat        >  1:1139482/1‑97 (MQ=21)
                                                                                           gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTAT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCag         >  1:50490/1‑99 (MQ=18)
                                                                                           gCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt         >  1:858963/1‑100 (MQ=18)
                                                                                           gCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt         >  1:3590016/1‑100 (MQ=18)
                                                                                           gCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt         >  1:1383822/1‑100 (MQ=18)
                                                                                           gCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt         >  2:1796265/1‑100 (MQ=17)
                                                                                            ccGGATAAGGCGTTCGCGCCGCATCCGGCCTACAAAAGTTTGCAAATTCAATAAATTGCAGAATTCAT‑‑GTAGGCCTGATAAGCGAAGCGCATCAGGCAtt        >  1:1516480/1‑100 (MQ=18)
                                                                                             cGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAttt       >  1:2923840/1‑100 (MQ=255)
                                                                                                aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg    <  2:3802578/100‑1 (MQ=255)
                                                                                                   |                                                                                                     
AGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCC  >  NC_000913/3705872‑3706069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: