Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,060,264 A→C 16.9% intergenic (+34/‑183) typA → / → yihL GTP‑binding protein/putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,060,2640AC16.9% 511.0 / 107.1 221intergenic (+34/‑183)typA/yihLGTP‑binding protein/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (80/103);  new base C (20/17);  total (100/121)
Fisher's exact test for biased strand distribution p-value = 2.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.30e-02

Only 100 of 222 total aligned reads displayed.

ATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATC  >  NC_000913/4060165‑4060362
                                                                                                   |                                                                                                   
 ttCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGAt                                                                                                    <  2:498754/100‑1 (MQ=255)
   cGTAAACGTCACCTGATGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATgg                                                                                                  >  2:1639788/1‑100 (MQ=255)
   cGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATgg                                                                                                  >  1:3485759/1‑100 (MQ=255)
   cGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATgg                                                                                                  >  1:1784814/1‑100 (MQ=255)
   cGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATg                                                                                                   <  1:2257670/99‑1 (MQ=255)
   cGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACAGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATgg                                                                                                  >  2:548995/1‑100 (MQ=255)
    gTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGc                                                                                                 >  1:3754659/1‑100 (MQ=255)
      aaaCGTGACCTCACGGAAAACGATCGTCTCCGGGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTACTCATAAAAAACCTGCCAGCGATgg                                                                                                  <  2:3137774/97‑1 (MQ=255)
      aaaCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCan                                                                                               <  2:1441660/100‑2 (MQ=255)
      aaaCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAg                                                                                               <  1:922936/100‑1 (MQ=255)
       aaCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAgg                                                                                              <  1:51291/100‑1 (MQ=255)
       aaCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAgg                                                                                              <  2:1551282/100‑1 (MQ=255)
       aaCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAgg                                                                                              >  1:3429044/1‑100 (MQ=255)
        aCGTCACCTGACGGAAATCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTAAAAAAAAAAAAAAAACCTGCCATCGCTGGCAGGt                                                                                            <  2:2760008/100‑1 (MQ=25)
        aCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGt                                                                                            <  1:2822258/100‑1 (MQ=255)
         cGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtt                                                                                           <  1:345862/100‑1 (MQ=255)
         cGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtt                                                                                           <  1:880738/100‑1 (MQ=255)
           tCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGtttt                                                                                         <  1:668273/100‑1 (MQ=255)
             aCCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtttttt                                                                                       >  1:336079/1‑100 (MQ=255)
              ccTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtttttt                                                                                       >  2:2858621/1‑99 (MQ=255)
               cTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtttttttt                                                                                     <  2:3662673/100‑1 (MQ=255)
               cTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttt                                                                                      <  2:1613961/99‑1 (MQ=255)
                tGACGGAAAACGATCTTCGCCGCGCCAAGCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGttttttatg                                                                                    <  2:1339743/100‑4 (MQ=37)
                tGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttt                                                                                    >  1:2531383/1‑100 (MQ=255)
                 gACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTg                                                                                   <  2:2414225/100‑1 (MQ=255)
                 gACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTg                                                                                   <  2:1596897/100‑1 (MQ=255)
                  aCGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGttttttatgac                                                                                  <  1:3659308/100‑6 (MQ=38)
                  aCGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGc                                                                                  <  2:2061305/100‑1 (MQ=255)
                   cGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGcc                                                                                 >  2:2244178/1‑100 (MQ=255)
                   cGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGcc                                                                                 >  2:737418/1‑100 (MQ=255)
                    ggAAAACGATCGTCGCCGCGTCAACCGCGAACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGttttttatgact                                                                                 <  2:1670468/99‑7 (MQ=25)
                     gAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTg                                                                               >  1:1351225/1‑100 (MQ=255)
                      aaaaCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGt                                                                              >  1:38925/1‑100 (MQ=255)
                       aaaCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTc                                                                             >  1:3134739/1‑100 (MQ=255)
                        aaCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCg                                                                            <  2:135319/100‑1 (MQ=255)
                          cGATCGTCGCCGCGCCACCCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCg                                                                            <  2:437023/98‑1 (MQ=255)
                          cGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTg                                                                          <  1:3553935/100‑1 (MQ=255)
                           gATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAACTTCGTCTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGCTTTTTTTTGCCTGTCCTGa                                                                         >  2:868946/1‑100 (MQ=255)
                             tcgtcgCCGCGCCAACCGTGCACCGAAAGTCGATTAATTTCTTTTGTTAGTCATAAAAAGCCTGCCAGCGATGGCAGGTTTTGCTTTGCCTGTCGTGAg                                                                        <  2:711944/99‑1 (MQ=255)
                             tcgtcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAgc                                                                       <  1:1891495/100‑1 (MQ=255)
                             tcgtcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAgc                                                                       <  1:1490548/100‑1 (MQ=255)
                               gtcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAgcgc                                                                     <  1:607821/100‑1 (MQ=255)
                                  gccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGcaca                                                                  >  1:2164783/1‑100 (MQ=255)
                                  gccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGcaca                                                                  <  2:1178034/100‑1 (MQ=255)
                                    cgcgCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACAcc                                                                <  2:2943463/100‑1 (MQ=255)
                                      cgcCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCaa                                                              >  1:2119190/1‑100 (MQ=255)
                                        ccAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTTGCTTGTGTTGGGCGCccacca                                                               >  1:3725681/1‑98 (MQ=255)
                                         cAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGc                                                           >  1:1908412/1‑100 (MQ=255)
                                            ccGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAGCCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAg                                                        <  2:1863153/100‑1 (MQ=255)
                                            ccGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAg                                                        >  1:2631400/1‑100 (MQ=255)
                                            ccGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAg                                                        <  2:557929/100‑1 (MQ=255)
                                             cgcgCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCACTGCGAg                                                        <  2:1125602/99‑1 (MQ=255)
                                             cgcgCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAg                                                        <  2:2902084/99‑1 (MQ=255)
                                              gcgcACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTa                                                      <  2:2186783/100‑1 (MQ=255)
                                              gcgcACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTa                                                      <  1:2340631/100‑1 (MQ=255)
                                              gcgcACCGAAAGACAATTAATTTCGTTTTTTTGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCCGTCGTGAGCGCACACCAATGCGAGTa                                                      <  2:352381/100‑1 (MQ=255)
                                               cgcACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTa                                                      <  1:1066086/100‑1 (MQ=255)
                                               cgcACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAt                                                     >  1:772061/1‑100 (MQ=255)
                                                 cACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAt                                                     >  1:1240848/1‑98 (MQ=255)
                                                 cACCGAAAGACGATTAATTTCGTTTTATAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAtct                                                   >  1:2328303/1‑100 (MQ=255)
                                                    cGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTccc                                                >  1:3233913/1‑100 (MQ=255)
                                                     gAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCt                                               >  2:1213479/1‑100 (MQ=255)
                                                       aaGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTta                                             >  1:3616103/1‑100 (MQ=255)
                                                       aaGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTta                                             <  1:650610/100‑1 (MQ=255)
                                                        aGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtat                                            <  2:3489759/100‑1 (MQ=255)
                                                           cGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAg                                         <  2:169302/100‑1 (MQ=255)
                                                           cGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAg                                         <  2:589923/100‑1 (MQ=255)
                                                            gATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGAACACCAATGCGAGTATCTCCCTTATAAg                                         >  2:41741/1‑99 (MQ=255)
                                                               tAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCt                                      <  2:1951423/100‑1 (MQ=255)
                                                                  tttCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTgg                                  <  2:2018394/100‑1 (MQ=255)
                                                                   ttCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGt                                 <  2:1495531/100‑1 (MQ=255)
                                                                   ttCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTACCTGTCGTGAGCGCACACCAATGCGTGTATCTCCCTTATATGTCTGTGGt                                 >  2:767689/1‑100 (MQ=255)
                                                                       ttttttAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACg                             <  2:2629996/100‑1 (MQ=255)
                                                                         ttttAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGATCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTc                           >  2:1311265/1‑100 (MQ=255)
                                                                         ttttAGTAATAAAAAACCTGCCAGACATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGGCTGTGGTTACGTc                           <  2:2354639/100‑1 (MQ=255)
                                                                              gcaaaaaaaaaCCTGCCATCGCTGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATag                      >  1:2389119/6‑100 (MQ=38)
                                                                               caaaaaaaaaCCTGCCATCGCTGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATaga                     >  1:2414999/5‑100 (MQ=255)
                                                                                cATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagag                    >  1:597492/1‑100 (MQ=255)
                                                                                 aaaaaaaaCCTGCCATCGCTGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagaga                   >  1:3073154/3‑100 (MQ=255)
                                                                                 aaaaaaaaCCTGCCATCGCTGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagaga                   >  1:3459250/3‑100 (MQ=255)
                                                                                 aTAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagaga                   <  2:1523476/100‑1 (MQ=255)
                                                                                  aaaaaaaCCTGCCATCGCTGGCAGGTTTTTTTTGCCTGACGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGc                 >  2:1827486/2‑100 (MQ=38)
                                                                                   aaaaaaCCTGCCATCGCTGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGc                 >  1:2207004/1‑100 (MQ=255)
                                                                                   aaaaaaCCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGc                 <  1:700910/100‑1 (MQ=255)
                                                                                     acaaCCTGCCATCGCTGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCtt               >  1:2593737/3‑100 (MQ=255)
                                                                                     aaaaCCTGCCATCGCTGGCAGGTTTTTTTTTTTTTTTCTTTATCGCACACCAATGCGCGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCtt               >  2:3447288/1‑100 (MQ=25)
                                                                                     aaaaCCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCtt               >  2:2814935/1‑100 (MQ=255)
                                                                                      aaaCCTGCCAGCGATGGCAGGTTTTTTTTTTTTTGTTTTTATCGCACACCAATGCGCGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCttt              >  2:2546819/1‑100 (MQ=255)
                                                                                      aaaCCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCttt              <  1:1655321/100‑1 (MQ=255)
                                                                                       aaCCTGCCATCGCTGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTg             >  2:3815271/1‑100 (MQ=255)
                                                                                       aaCCTGCCATCGCTGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTAGAAGTCTGGGGTTACGTCAATAGAGAGCtt               >  2:1469801/1‑98 (MQ=38)
                                                                                       aaCCTGCCAGCGATGGCAGGTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGt            <  1:103045/100‑1 (MQ=255)
                                                                                         ccTGCCATCGCTGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTc           >  1:3265090/1‑100 (MQ=255)
                                                                                         ccTGCCATCGCTGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTc           >  1:3195262/1‑100 (MQ=255)
                                                                                          cgatctgCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGGTTTGTca          <  1:1996974/94‑1 (MQ=255)
                                                                                           tGCCAGCGTTGGAAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTcac         <  1:2680846/100‑1 (MQ=255)
                                                                                            gCCAGCGATGGCAGGTTTTTTTTTTTTTTTTTTTTACGCACACCAATGCGAGTATCTCTCTTATAAGTCTGTGGTTACGTCGATAGAGAGCTTTGTcaca        >  2:3407457/1‑100 (MQ=255)
                                                                                             ccAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACaa       <  2:667004/100‑1 (MQ=255)
                                                                                               aGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAAtt     <  1:1755813/100‑1 (MQ=255)
                                                                                                  gATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATc  <  1:2955784/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
ATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATC  >  NC_000913/4060165‑4060362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: