BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	632,971	C→T	100%	A169A (GCG→GCA)	ybdH ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	632,971	0	C	T	97.9%	453.8 / ‑0.7	139	A169A (GCG→GCA)	ybdH	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (1/2); new base T (67/69); total (68/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.32e-01

Only 100 of 141 total aligned reads displayed.

ATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGT  >  NC_000913/632875‑633067

attattGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACGACCGCTTCAAACCATTTCGCCAGCGTGTCACCGATCCCTGcc                                                                                               >  2:2676097/1‑100 (MQ=255)

attattGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGcc                                                                                               >  1:3101760/1‑100 (MQ=255)

 ttattGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCca                                                                                              <  1:3033243/100‑1 (MQ=255)

     tGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCcagcag                                                                                          <  2:3747722/100‑1 (MQ=255)

     tGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCcagcag                                                                                          <  1:2945598/100‑1 (MQ=255)

     tGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCcagcag                                                                                          >  1:2157087/1‑100 (MQ=255)

     tGATCCCCAGTCGCACGGGTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCcagcag                                                                                          <  2:3532047/100‑1 (MQ=255)

       aTCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGat                                                                                        <  2:1233940/100‑1 (MQ=255)

        tCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGata                                                                                       >  1:1760949/1‑100 (MQ=255)

        tCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGata                                                                                       >  1:3302250/1‑100 (MQ=255)

         ccccAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGatat                                                                                      >  1:3060563/1‑100 (MQ=255)

          cccAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATAtt                                                                                     >  1:3567157/1‑100 (MQ=255)

          cccAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATAtt                                                                                     >  1:2705596/1‑100 (MQ=255)

          cccAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATAtt                                                                                     >  1:963327/1‑100 (MQ=255)

          cccAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATAtt                                                                                     >  1:3245239/1‑100 (MQ=255)

            cAGTCGCACGGTTAGCGGCAACGTTTCTGGGTGCGGAGCCAGCACCACCGCCTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATAttgt                                                                                   <  2:1647251/100‑1 (MQ=255)

             aGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATAttgtt                                                                                  <  2:2760135/100‑1 (MQ=255)

              gTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATAttgttg                                                                                 >  1:3732658/1‑100 (MQ=255)

                cGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCg                                                                               >  2:346543/1‑100 (MQ=255)

                   aCGGTTAGCGGCAACGTTTTTGGTCGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCg                                                                               <  2:3210419/97‑1 (MQ=255)

                   aCGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATGTTGTTGCGGt                                                                             <  2:3270870/99‑1 (MQ=255)

                   aCGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGt                                                                             >  2:2524280/1‑99 (MQ=255)

                   aCGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGGGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTg                                                                            <  2:3589015/100‑1 (MQ=255)

                    cGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGc                                                                           >  2:142794/1‑100 (MQ=255)

                         aGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGa                                                                      >  2:2141538/1‑100 (MQ=255)

                          gCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAg                                                                     <  1:2414910/100‑1 (MQ=255)

                          gCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAg                                                                     >  1:528659/1‑100 (MQ=255)

                          gCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAg                                                                     >  2:644629/1‑100 (MQ=255)

                           nggCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCGTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAgg                                                                    >  2:490941/2‑100 (MQ=255)

                           cGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAgg                                                                    <  2:1026138/100‑1 (MQ=255)

                            ggCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGa                                                                   <  1:3615335/100‑1 (MQ=255)

                            ggCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGACCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGa                                                                   >  2:161477/1‑100 (MQ=255)

                                aCGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAAt                                                               <  2:2707590/100‑1 (MQ=255)

                                  gtctCTGGTTGCGGAGCCAGCACCACCGCTTCACACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAAtct                                                             <  2:951681/97‑1 (MQ=255)

                                   tttCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAAtctc                                                            <  2:1467331/100‑1 (MQ=255)

                                    ttCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTcc                                                           <  1:1575974/100‑1 (MQ=255)

                                     tCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCg                                                          <  1:1952276/100‑1 (MQ=255)

                                       tGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGt                                                        >  2:2894644/1‑100 (MQ=255)

                                         gTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTc                                                      >  2:1934601/1‑100 (MQ=255)

                                          ttGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTcc                                                     <  1:2141551/100‑1 (MQ=255)

                                           tGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTcca                                                    >  1:1167381/1‑100 (MQ=255)

                                           tGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTcca                                                    >  2:3056580/1‑100 (MQ=255)

                                           tGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTcca                                                    <  1:2798551/100‑1 (MQ=255)

                                            gCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTccac                                                   <  2:1630813/100‑1 (MQ=255)

                                            gCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTccac                                                   <  1:2800659/100‑1 (MQ=255)

                                            gCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTccac                                                   <  1:1954303/100‑1 (MQ=255)

                                             cGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTccacc                                                  <  1:1996629/100‑1 (MQ=255)

                                              ggAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTccacca                                                 >  2:2795616/1‑100 (MQ=255)

                                                 gCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCa                                              >  2:2304335/1‑100 (MQ=255)

                                                 gCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCa                                              <  1:2231992/100‑1 (MQ=255)

                                                   cAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCAcc                                            <  2:2764345/100‑1 (MQ=255)

                                                   cAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCAcc                                            <  1:2784695/100‑1 (MQ=255)

                                                   cAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCAACCAGCAc                                             >  2:1514268/1‑99 (MQ=255)

                                                   cAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCAcc                                            <  2:2475647/100‑1 (MQ=255)

                                                    aGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCa                                           <  1:3464673/100‑1 (MQ=255)

                                                    aGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCa                                           <  1:1667283/100‑1 (MQ=255)

                                                     gCACCTCCGCTTCATACCATTTCGCCAGCGGGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCAcc                                            <  2:858595/98‑1 (MQ=255)

                                                        ccaccGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATaaa                                       >  1:2705501/1‑100 (MQ=255)

                                                         caccGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATaaaa                                      >  1:3421765/1‑100 (MQ=255)

                                                         caccGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATaaaa                                      >  1:614645/1‑100 (MQ=255)

                                                         caccGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATaaaa                                      >  1:3732547/1‑100 (MQ=255)

                                                         caccGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCTCCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATacaa                                      <  1:1708389/100‑1 (MQ=255)

                                                              cTTCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGc                                 <  1:1059144/100‑1 (MQ=255)

                                                              cTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATCTTGTTGCGGTGCATTGCGGATAATCCCCGGTTCCACCAGCACCATAAAATTGGc                                 >  2:1874676/1‑100 (MQ=255)

                                                               ttCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGcg                                <  2:1676818/100‑1 (MQ=255)

                                                               ttCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGcg                                <  2:3564480/100‑1 (MQ=255)

                                                                tCATACCATTTCGCCAGCGTGTCACTGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGcgt                               <  2:391163/100‑1 (MQ=255)

                                                                tCATACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGcgt                               <  1:3204105/100‑1 (MQ=255)

                                                                tCATACCATTACGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGcg                                <  2:1581885/99‑1 (MQ=255)

                                                                  aTACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGcgtcg                             >  1:1544571/1‑100 (MQ=255)

                                                                   tACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGcgtcgt                            >  2:3711639/1‑100 (MQ=255)

                                                                   tACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGcgtcgt                            >  1:2510066/1‑100 (MQ=255)

                                                                   tACCATTTCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTAGcgtcg                             >  1:1364512/1‑99 (MQ=255)

                                                                        tttCGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGaaa                       <  2:533378/100‑1 (MQ=255)

                                                                           cGCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAAtc                    >  1:3094228/1‑100 (MQ=255)

                                                                            gCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAAtct                   <  1:2489050/100‑1 (MQ=255)

                                                                            gCCAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAAtct                   >  2:2555242/1‑100 (MQ=255)

                                                                             ccAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAAtctc                  >  1:1258005/1‑100 (MQ=255)

                                                                              cAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCa                 <  1:8886/100‑1 (MQ=255)

                                                                              cAGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCa                 <  1:2860663/100‑1 (MQ=255)

                                                                               aGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCAt                <  2:1856904/100‑1 (MQ=255)

                                                                               aGCGTGTCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCAt                <  1:2882912/100‑1 (MQ=255)

                                                                                  gtgtCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAAt             >  2:1124693/1‑100 (MQ=255)

                                                                                    gtCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAACATTGGCGTCGTCGAAAATCTCATAATGc           >  2:3413360/1‑100 (MQ=255)

                                                                                    gtCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGc           >  2:1900200/1‑100 (MQ=255)

                                                                                    gtCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGc           >  2:165386/1‑100 (MQ=255)

                                                                                    gtCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGc           <  1:3415082/100‑1 (MQ=255)

                                                                                    gtCACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAACCTCATAATGc           >  2:720481/1‑100 (MQ=255)

                                                                                      cACCGATCTCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAg         <  1:234541/100‑1 (MQ=255)

                                                                                      cACCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAg         >  1:727105/1‑100 (MQ=255)

                                                                                      cACCGATCCCTGCCAGCAGATATTGTTGCGGTGCACTGAGGATAATCTCCCGTTCAACCAGCACCATAAAATTGGCGCCGTCGAAAATCTCATAATgtcg         >  2:1179785/1‑97 (MQ=255)

                                                                                       aCCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAgc        >  2:2146675/1‑100 (MQ=255)

                                                                                       aCCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAgc        >  2:1446880/1‑100 (MQ=255)

                                                                                       aCCGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAgc        >  1:3316984/1‑100 (MQ=255)

                                                                                        ccGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAgcg       <  2:1074978/100‑1 (MQ=255)

                                                                                        ccGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAgcg       >  1:1081203/1‑100 (MQ=255)

                                                                                        ccGATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAgcg       <  1:3094311/100‑1 (MQ=255)

                                                                                          gATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGcc     <  2:2867339/100‑1 (MQ=255)

                                                                                          gATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGcc     >  2:2248786/1‑100 (MQ=255)

                                                                                          gATCCCTGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGcc     <  1:941225/100‑1 (MQ=255)

ATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGT  >  NC_000913/632875‑633067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`