BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,077,116	T→C	100%	E589E (GAA→GAG)	putA ←	fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,077,116	0	T	C	100.0%	405.2 / NA	115	E589E (GAA→GAG)	putA	fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (34/81); total (34/81)

Only 100 of 119 total aligned reads displayed.

GCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCA  >  NC_000913/1077019‑1077210

gCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTtctc                                                                                              >  2:3202550/1‑100 (MQ=255)

gCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTtctc                                                                                              <  1:1093025/100‑1 (MQ=255)

 cccTGGCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTctct                                                                                             <  2:2890695/100‑1 (MQ=255)

 cccTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTtctcn                                                                                             <  2:1580477/100‑2 (MQ=255)

  ccTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTa                                                                                            <  2:1611112/100‑1 (MQ=255)

     gcggAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTAc                                                                                           <  2:2941925/95‑1 (MQ=255)

     gCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTAcag                                                                                         <  1:772762/100‑1 (MQ=255)

      ccGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTAcagc                                                                                        >  1:1999617/1‑100 (MQ=255)

       cGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTAcagca                                                                                       <  1:2627553/100‑1 (MQ=255)

        gAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTAcagcag                                                                                      <  2:719153/100‑1 (MQ=255)

           ttGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGa                                                                                   <  1:1471749/100‑1 (MQ=255)

            tGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGCTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGAc                                                                                  >  2:1534423/1‑100 (MQ=255)

             gTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGAcc                                                                                 <  1:2303858/100‑1 (MQ=255)

             gTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGAcc                                                                                 >  1:2812078/1‑100 (MQ=255)

             gTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGAcc                                                                                 <  2:1926811/100‑1 (MQ=255)

             gTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGAcc                                                                                 <  1:2478812/100‑1 (MQ=255)

             gTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGAcc                                                                                 >  2:1010761/1‑100 (MQ=255)

             gTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGAcc                                                                                 <  1:3409854/100‑1 (MQ=255)

             gTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGAcc                                                                                 <  1:3615064/100‑1 (MQ=255)

             gTCGCGCCCGTGACCGTAAAAATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGAcc                                                                                 >  2:2325147/1‑100 (MQ=255)

              tCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGTGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCg                                                                                <  1:670178/100‑1 (MQ=255)

              tCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCg                                                                                <  2:3683499/100‑1 (MQ=255)

                 cgcCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGAt                                                                             <  1:100092/100‑1 (MQ=255)

                 cgcCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAAGCCAGTTTGCCGTTCCTGTTTCGCCAGTTTCTCTACAGCATTGACggg                                                                               >  2:3320892/1‑98 (MQ=255)

                  gcCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATc                                                                            <  1:758162/100‑1 (MQ=255)

                     cGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGc                                                                         <  1:786582/100‑1 (MQ=255)

                     cGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGc                                                                         <  1:243708/100‑1 (MQ=255)

                      gTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCg                                                                        <  2:2784508/100‑1 (MQ=255)

                      gTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCg                                                                        <  1:1077538/100‑1 (MQ=255)

                         aCCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGAcc                                                                     <  1:3196768/100‑1 (MQ=255)

                         aCCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGAcc                                                                     <  1:1068413/100‑1 (MQ=255)

                         aCCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGAc                                                                      <  1:314710/99‑1 (MQ=255)

                          ccGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCa                                                                    >  2:237407/1‑100 (MQ=255)

                           cGTAAAGATCGCTCGGCAGGGGAATCTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGATCAg                                                                   >  2:1247228/1‑100 (MQ=255)

                           cGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAg                                                                   <  1:3416236/100‑1 (MQ=255)

                            gTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAg                                                                   <  2:1369241/99‑1 (MQ=255)

                              aaaGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTc                                                                >  2:1640166/1‑100 (MQ=255)

                              aaaGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTc                                                                >  1:2006840/1‑100 (MQ=255)

                               aaGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCa                                                               <  2:1238437/100‑1 (MQ=255)

                               aaGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCa                                                               <  2:730488/100‑1 (MQ=255)

                               aaGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAAGCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTc                                                                <  1:1342419/99‑1 (MQ=255)

                                aGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCAt                                                              <  1:3789906/100‑1 (MQ=255)

                                aGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCAt                                                              <  2:3760642/100‑1 (MQ=255)

                                 gATCGCGCGGCGGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATc                                                             <  2:2098837/100‑1 (MQ=255)

                                  aTCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATcc                                                            <  1:4043/100‑1 (MQ=255)

                                    cgcgcgGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTCCCTGTTGCGCCCGGTTCGCTACAGCAGTGACCGGATCGGCGACCAGtggatgc                                                            >  2:3104357/1‑92 (MQ=255)

                                        cgGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGc                                                      >  1:1387373/1‑100 (MQ=255)

                                        cgGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGc                                                      >  1:1086656/1‑100 (MQ=255)

                                        cgGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGc                                                      <  2:2390707/100‑1 (MQ=255)

                                         ggCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCa                                                     <  2:823588/100‑1 (MQ=255)

                                         ggCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCa                                                     <  2:3067994/100‑1 (MQ=255)

                                           cAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCATTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCaaa                                                   <  1:1866360/100‑1 (MQ=255)

                                            aGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCaaan                                                  <  2:3752222/100‑2 (MQ=255)

                                            aGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAag                                                  <  1:883813/100‑1 (MQ=255)

                                              gggAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAagag                                                <  1:1925481/100‑1 (MQ=255)

                                              gggAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAagag                                                <  1:1142389/100‑1 (MQ=255)

                                              gggAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAagag                                                <  2:3198690/100‑1 (MQ=255)

                                              gggAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAagag                                                <  2:3524631/100‑1 (MQ=255)

                                               gtaaTTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAgg                                               >  1:3218614/3‑100 (MQ=255)

                                               ggAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAgg                                               <  2:2467091/100‑1 (MQ=255)

                                               ggAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAgg                                               <  2:3368204/100‑1 (MQ=255)

                                                 aaTTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGgtg                                             >  1:1322803/1‑100 (MQ=255)

                                                     ttCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCgg                                         <  2:2397029/100‑1 (MQ=255)

                                                              ggTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGt                                <  2:469434/100‑1 (MQ=255)

                                                              ggTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGt                                <  1:1738839/100‑1 (MQ=255)

                                                               gTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGtt                               <  2:947751/100‑1 (MQ=255)

                                                               gTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGtt                               <  1:2365893/100‑1 (MQ=255)

                                                               gTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGtt                               <  2:946056/100‑1 (MQ=255)

                                                                 aaTCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTaa                             <  1:1853068/100‑1 (MQ=255)

                                                                  aTCCAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAAc                            <  1:2802753/100‑1 (MQ=255)

                                                                     cAGTTTGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACaaa                         <  1:882483/100‑1 (MQ=255)

                                                                       gTTTGCCCTTCCTGCTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCAACCAGTGGCAACGAGGTGTCGGCAATACGGGTAACCAACg                       >  2:2684894/1‑100 (MQ=255)

                                                                        tttGCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGa                      <  1:1361602/100‑1 (MQ=255)

                                                                           gCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGgt                   >  2:2001683/1‑100 (MQ=255)

                                                                           gCCCTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGgt                   >  2:3143711/1‑100 (MQ=255)

                                                                            cccTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGgtg                  <  2:34212/100‑1 (MQ=255)

                                                                            cccTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGgtg                  <  1:2073921/100‑1 (MQ=255)

                                                                            cccTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGgtg                  <  1:2666965/100‑1 (MQ=255)

                                                                             ccTTCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGgtgt                 >  2:1172524/1‑100 (MQ=255)

                                                                               ttCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTa               <  1:2217755/100‑1 (MQ=255)

                                                                                tCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAg              <  1:698144/100‑1 (MQ=255)

                                                                                tCCTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAg              <  2:119939/100‑1 (MQ=255)

                                                                                 ccTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGc             <  2:3528735/100‑1 (MQ=255)

                                                                                  cTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGtt                <  2:3592362/96‑1 (MQ=255)

                                                                                  cTGTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCa            >  2:3696594/1‑100 (MQ=255)

                                                                                    gTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCAcc          <  2:1839811/100‑1 (MQ=255)

                                                                                    gTTGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCAcc          >  2:101189/1‑100 (MQ=255)

                                                                                      tGCGCCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGt        >  1:2006201/1‑100 (MQ=255)

                                                                                       gcgcCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGtt       >  2:3735230/1‑100 (MQ=255)

                                                                                        cgcCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGttt      >  1:2319042/1‑100 (MQ=255)

                                                                                        cgcCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGttt      <  1:1680003/100‑1 (MQ=255)

                                                                                        cgcCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGttt      >  1:1374631/1‑100 (MQ=255)

                                                                                        cgcCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGttt      <  1:49023/100‑1 (MQ=255)

                                                                                         gcCAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGtttt     <  1:3025853/100‑1 (MQ=255)

                                                                                          ccAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTc    <  2:1678964/100‑1 (MQ=255)

                                                                                          ccAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTc    >  2:1528441/1‑100 (MQ=255)

                                                                                           cAGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTcc   >  1:1589073/1‑100 (MQ=255)

                                                                                            aGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATCCGGTTAACAAACGAGGGGTTAGCACCGTTTTcc   >  2:2050407/1‑99 (MQ=255)

                                                                                            aGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCca  <  1:2666542/100‑1 (MQ=255)

                                                                                            aGTTTCTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCca  >  1:2205861/1‑100 (MQ=255)

GCCCTGCCGAGTTGTCGCGCCCGTGACCGTAAAGATCGCGCGGCAGGGGAATTTTCGGATGCGGTAATCCAGTTTGCCCTTCCTGTTGCGCCAGTTTTTCTACAGCAGTGACCGGATCGGCGACCAGTTCATCCAGTGGCAAAGAGGTGTCGGCAATACGGTTAACAAACGAGGTGTTAGCACCGTTTTCCA  >  NC_000913/1077019‑1077210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`