Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,335,684 G→A 16.6% intergenic (+226/‑147) yciX → / → acnA uncharacterized protein/aconitate hydratase 1; aconitase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,335,6840GA16.6% 294.1 / 42.5 126intergenic (+226/‑147)yciX/acnAuncharacterized protein/aconitate hydratase 1; aconitase A
Reads supporting (aligned to +/- strand):  ref base G (51/54);  new base A (9/12);  total (60/66)
Fisher's exact test for biased strand distribution p-value = 8.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 130 total aligned reads displayed.

ATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATT  >  NC_000913/1335587‑1335781
                                                                                                 |                                                                                                 
atGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTgcggc                                                                                                 >  2:850194/1‑100 (MQ=255)
  gAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGcggcgg                                                                                               >  1:3029045/1‑100 (MQ=255)
    aaTATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCgggg                                                                                             <  2:3252959/100‑1 (MQ=255)
       atTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCCGCAGCGGGGtca                                                                                          <  1:2354240/100‑3 (MQ=255)
       atTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCCGCAGCGGGGtc                                                                                           <  2:935035/99‑2 (MQ=255)
        tgggTCCTGGGTGGGCGCGTTTCCGCTGTCGATGCACTTCCGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGCCGGGGTTg                                                                                          <  2:2180018/97‑1 (MQ=255)
         tGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTc                                                                                        <  1:2683120/100‑1 (MQ=255)
         tGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTc                                                                                        >  2:553542/1‑100 (MQ=255)
         tGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCCGCAGCGGGGtcaag                                                                                        <  2:1438416/100‑5 (MQ=255)
         tGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTAACCCCGCCGCAGCGGGGtcaag                                                                                        <  2:327820/100‑5 (MQ=255)
          ggTCCTGGATTGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCCGCAGCGGGGtcaagg                                                                                       <  2:2518567/100‑6 (MQ=255)
          ggTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCa                                                                                       >  1:16939/1‑100 (MQ=255)
          ggTCCTGGATGGGCGCGTTTTCTCTGTCGATGCCCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCCGCAGCGGGGtcaac                                                                                        <  2:3624500/99‑5 (MQ=255)
           gTCCTGGATGGGGGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGACCCGCTCTGGATCATTACTAACTGATCCTTGACCCCGCCGCAGCGGGGtcaagga                                                                                      <  2:349491/100‑7 (MQ=255)
           gTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTATGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCAt                                                                                      >  1:2693195/1‑100 (MQ=255)
            tCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCAtt                                                                                     <  1:1089882/100‑1 (MQ=255)
             ccTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCAttt                                                                                    >  1:1711406/1‑100 (MQ=255)
                ggATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCAtttgct                                                                                 <  1:1426513/100‑1 (MQ=255)
                 gATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCAtttgctt                                                                                >  1:3298730/1‑100 (MQ=255)
                 gATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCAtttgctt                                                                                >  2:2813177/1‑100 (MQ=255)
                    gggCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCAtttgctttgc                                                                             <  1:2343176/100‑1 (MQ=255)
                    gggCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCAtttgctttgc                                                                             <  2:3553925/100‑1 (MQ=255)
                     ggCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGcc                                                                            >  1:1737094/1‑100 (MQ=255)
                     ggCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGcc                                                                            <  1:699194/100‑1 (MQ=255)
                      gcgcGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCca                                                                           <  2:231336/100‑1 (MQ=255)
                      gcgcGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCca                                                                           >  1:1471648/1‑100 (MQ=255)
                        gcgTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCcaca                                                                         <  1:819555/100‑1 (MQ=255)
                           ttttCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAgg                                                                      <  1:3767927/100‑1 (MQ=255)
                           ttttCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAgg                                                                      <  1:2183648/100‑1 (MQ=255)
                           ttttCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAgg                                                                      <  1:2043632/100‑1 (MQ=255)
                             ttCTCTGTCGATGCTCTTCTGGGCCGATCTCCTCTGTATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGtt                                                                    <  1:1364551/100‑1 (MQ=255)
                               ctctGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTTCCACAAGGTTtc                                                                  <  1:2516001/100‑1 (MQ=255)
                               ctctGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTtc                                                                  >  1:2760083/1‑100 (MQ=255)
                               ctctGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTtc                                                                  >  1:3548654/1‑100 (MQ=255)
                               ctctGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCGGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTtc                                                                  >  2:1242260/1‑100 (MQ=255)
                                tctGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTtct                                                                 <  1:1259553/100‑1 (MQ=255)
                                  tGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTctcc                                                               <  1:3371570/100‑1 (MQ=255)
                                  tGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTctcc                                                               <  1:130064/100‑1 (MQ=255)
                                  tGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTctcc                                                               <  2:62396/100‑1 (MQ=255)
                                  tGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTctcc                                                               >  1:3070703/1‑100 (MQ=255)
                                   gTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTctcct                                                              <  1:3817745/100‑1 (MQ=255)
                                      gATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCtt                                                           <  1:2500973/100‑1 (MQ=255)
                                       aTGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCttt                                                          <  1:2187083/100‑1 (MQ=255)
                                        tGCGCTTCTGGGCGGAGCTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCtttt                                                         <  2:1415482/100‑1 (MQ=255)
                                         gCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTa                                                        <  2:2992176/100‑1 (MQ=255)
                                         gCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTa                                                        <  1:3000631/100‑1 (MQ=255)
                                          ctgtgctGGGCCCAACTCCTCGGGGTAGTTACGCAGTGATCCTTGACCCCGCTGCGGGGGGGTTGTCATTTGCTTAGCCACAAGGTTTCTCCTCttt                                                          <  2:3078054/92‑1 (MQ=255)
                                          ctctTGTGGGCCGAACTCCTCTGGGTGAGTACTCACGGAGCCGTGACCCCCCTGCGGCGGTGTTGTCATGTGCTTTGCCACAAGGGTTCTCCTCtgttn                                                        <  2:2359969/99‑2 (MQ=255)
                                          ctctTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGTGGAGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTAt                                                       >  2:2685575/1‑100 (MQ=255)
                                               ctGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAAtt                                                  >  1:1930809/1‑100 (MQ=255)
                                                tGGGCCGAACTACTCTGGATCAGTACTCACTGATCCTTGAGCACGCTGCGGCGGGGGTGTCATTTACTTTGCCACAAGGTTTCTCCTCTTTTATCAAtt                                                  >  2:2378290/1‑99 (MQ=255)
                                                 gggCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTg                                                >  1:1499748/1‑100 (MQ=255)
                                                 gggCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTg                                                <  2:3061742/100‑1 (MQ=255)
                                                 gggCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTg                                                <  2:2184994/100‑1 (MQ=255)
                                                   gCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTggg                                              >  1:3630161/1‑100 (MQ=255)
                                                   gCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTggg                                              <  2:2653935/100‑1 (MQ=255)
                                                    ccGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGgt                                             <  2:544056/100‑1 (MQ=255)
                                                      gAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGgttg                                           <  2:3452658/100‑1 (MQ=255)
                                                       aaCTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGgttgt                                          <  1:684854/100‑1 (MQ=255)
                                                       aaCTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCGGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTGCTCCTCGTTTATCAATTTGGgttgt                                          <  2:1524797/100‑1 (MQ=255)
                                                         ctcctcTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTa                                        <  2:24442/100‑1 (MQ=255)
                                                            ctctGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCa                                     <  1:2458166/100‑1 (MQ=255)
                                                             tctGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCaa                                    >  1:1969728/1‑100 (MQ=255)
                                                               tGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTACCAAAt                                  >  2:1578767/1‑100 (MQ=255)
                                                                ggATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGTTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATc                                 <  2:2045024/100‑1 (MQ=255)
                                                                 gATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGATGTTATCAAATc                                 >  2:3572409/1‑99 (MQ=255)
                                                                  aTCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGt                               >  1:1401907/1‑100 (MQ=255)
                                                                        aCTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTAcgcg                         <  2:2141329/100‑1 (MQ=255)
                                                                         cTCACTGATCCTTGACCCCGCTGCGGCGGTGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTAcgcg                         <  2:1175806/99‑1 (MQ=255)
                                                                         cTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCCTTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATGGTTAcgc                          >  2:1998006/1‑98 (MQ=255)
                                                                         cTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGa                        >  1:1280103/1‑100 (MQ=255)
                                                                         cTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGa                        >  1:3305917/1‑100 (MQ=255)
                                                                          tCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGAt                       <  2:2815054/100‑1 (MQ=255)
                                                                           cACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATg                      >  1:1560518/1‑100 (MQ=255)
                                                                           cACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATg                      >  2:3151478/1‑100 (MQ=255)
                                                                           cACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGAGATg                      >  2:1797908/1‑100 (MQ=255)
                                                                             cTGATCCTTGACCCCTCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGtt                    >  1:923065/1‑100 (MQ=255)
                                                                             cTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGtt                    >  1:854729/1‑100 (MQ=255)
                                                                             cTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGtt                    >  2:1764513/1‑100 (MQ=255)
                                                                             cTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGtt                    <  1:2540647/100‑1 (MQ=255)
                                                                             cTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGtt                    >  1:2478525/1‑100 (MQ=255)
                                                                              tGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGCTGttt                   >  2:2100805/1‑100 (MQ=255)
                                                                               gATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtg                  <  2:3072162/100‑1 (MQ=255)
                                                                               aaatgacgACCCCGCCGCAGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtg                  >  1:2010275/8‑100 (MQ=255)
                                                                               aaatgacaaCCCCGCCGCAGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtg                  >  1:40168/9‑100 (MQ=38)
                                                                                aTCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgt                 >  2:3015129/1‑100 (MQ=255)
                                                                                 tCCTTGACCCCGCTGTGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgtg                <  1:3766870/100‑1 (MQ=255)
                                                                                  tgacaaCCCCGCCGCAGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTgtgt               >  2:3641722/6‑100 (MQ=255)
                                                                                   cTTGACCCCACTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGtt              >  2:2767397/1‑100 (MQ=255)
                                                                                    acaaCCCCGCCGCAGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTa             >  2:1329315/4‑100 (MQ=255)
                                                                                    acaaCCCCGCCGCAGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTa             >  2:1913832/4‑100 (MQ=255)
                                                                                     caaCCCCGCCGCAGCGGGTTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGTGTTGTTATCAAATCGTTACGCGATTTTTgtgt               >  2:1684823/3‑97 (MQ=255)
                                                                                     caaCCCCGCCGCAGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTAt            >  1:3328372/3‑100 (MQ=255)
                                                                                       aCCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCt          >  2:563842/1‑100 (MQ=255)
                                                                                        ccccGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCtt         >  1:67059/1‑100 (MQ=255)
                                                                                        ccccGCCGCAGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCtt         >  1:2142804/1‑100 (MQ=255)
                                                                                         cccGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCCAATCGTTACGCGATGTTTGTGTTATCttt        >  2:3491969/1‑100 (MQ=255)
                                                                                              tgctgcGGCTTTGTCATTTGCTTTGCTACAAGTTTTCTCCTCTTTTATCTATTGTGGTTTTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAata    <  2:1266123/99‑1 (MQ=255)
                                                                                              tGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAatat   <  2:1969717/100‑1 (MQ=255)
                                                                                               gcggcgGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATAtt  >  1:3478605/1‑100 (MQ=255)
                                                                                                 |                                                                                                 
ATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATT  >  NC_000913/1335587‑1335781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: