Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,344,036 G→C 15.1% T104S (ACT→AGT)  yciT ← global regulator of transcription; DeoR family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,344,0360GC15.1% 367.5 / 51.8 132T104S (ACT→AGT) yciTglobal regulator of transcription; DeoR family
Reads supporting (aligned to +/- strand):  ref base G (47/65);  new base C (11/9);  total (58/74)
Fisher's exact test for biased strand distribution p-value = 3.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

Only 100 of 141 total aligned reads displayed.

CCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCG  >  NC_000913/1343937‑1344135
                                                                                                   |                                                                                                   
ccACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCagag                                                                                                     <  1:2489156/100‑1 (MQ=255)
ccACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGATGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCagag                                                                                                     >  2:1165084/1‑100 (MQ=255)
 cACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGt                                                                                                    <  1:803780/100‑1 (MQ=255)
        cAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCg                                                                                             <  2:885282/100‑1 (MQ=255)
        cAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCg                                                                                             <  1:1489290/100‑1 (MQ=255)
        cAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCg                                                                                             <  2:3435123/100‑1 (MQ=255)
         aaaaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGa                                                                                            >  1:1914045/1‑100 (MQ=255)
         aaaaTAACTTCACAAGGCGCGTCTTTCAGCAAATAAGCGATGTAGCTACTGACCGTGCTGATAGTGACATTATTCTTCTGCTGGCAAAGAGTACggg                                                                                               >  2:932269/1‑97 (MQ=255)
           aaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCGGACTCTGGGCGAgc                                                                                          <  1:2291133/100‑2 (MQ=255)
           aaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGt                                                                                          <  2:777635/100‑1 (MQ=255)
           aaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGt                                                                                          <  2:1486678/100‑1 (MQ=255)
           aaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGt                                                                                          >  1:1526794/1‑100 (MQ=255)
            atctcTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTACTCGCCCGGACTCTGGGCGAgca                                                                                         <  2:641676/96‑3 (MQ=38)
            aTAACTGCACGTGTCGCGGGTTTCAGCGAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTCTTCTGCTCGCCCAGAGTCCGGGCg                                                                                             <  2:3530666/95‑1 (MQ=255)
              aaCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCGGACTCTGGGCGAGCAga                                                                                       <  2:824083/100‑2 (MQ=255)
              aaCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAgg                                                                                       >  2:2271269/1‑100 (MQ=255)
               aCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGaggagg                                                                                       >  2:3110242/1‑99 (MQ=255)
               aCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAggg                                                                                      <  1:3344838/100‑1 (MQ=255)
               aCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAggg                                                                                      <  1:1423487/100‑1 (MQ=255)
                cTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTTTTTTTTTTTCCCAGAGTCGGGGCGAGTAGGGc                                                                                     >  2:875714/1‑100 (MQ=255)
                cTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGc                                                                                     >  1:292886/1‑100 (MQ=255)
                 ttCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCa                                                                                    >  2:3617034/1‑100 (MQ=255)
                 tacacgAGGCTCGTCTTTAAGCAATTGAGCGATGTAGCTGCTGCCCGTGGTGAGAGTGACATTTTTCTTCTGCTCGCCCGGACGCTGGGCGAgcagaag                                                                                     <  2:1669810/97‑7 (MQ=255)
                  tCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCAt                                                                                   <  2:3298249/100‑1 (MQ=255)
                     caAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgc                                                                                <  1:1596075/100‑1 (MQ=255)
                     caAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgc                                                                                <  1:1345622/100‑1 (MQ=255)
                        ggCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgctgc                                                                             <  2:266120/100‑1 (MQ=255)
                        ggCGAGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTGCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgctgc                                                                             <  2:254522/100‑1 (MQ=255)
                          cgcgTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgctgctg                                                                           <  1:3806229/100‑1 (MQ=255)
                          cgcgTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgctgctg                                                                           >  1:449949/1‑100 (MQ=255)
                           gcgTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgctgctgc                                                                          <  1:3597724/100‑1 (MQ=255)
                           gcgTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgctgctgc                                                                          >  2:1961948/1‑100 (MQ=255)
                            cgTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGcc                                                                         <  2:3438280/100‑1 (MQ=255)
                               cTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAtt                                                                      >  1:1121054/1‑100 (MQ=255)
                                 ttCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAtttt                                                                    >  1:1599469/1‑100 (MQ=255)
                                 ttCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAtttt                                                                    <  1:3534105/100‑1 (MQ=255)
                                  tCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTc                                                                   <  2:1262324/100‑1 (MQ=255)
                                  tCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCGGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTc                                                                   <  2:371202/100‑1 (MQ=255)
                                  tCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGGGACATTTTTCTTCTGCTCGCCCAGAGTCCAGGCGAGTAGGGCATTGCTGCTGCCATTTTc                                                                   <  2:976600/100‑1 (MQ=255)
                                   cAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCg                                                                  <  2:2767341/100‑1 (MQ=255)
                                   cAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCg                                                                  <  2:3645843/100‑1 (MQ=255)
                                    aGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGTTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGa                                                                 <  1:236130/100‑1 (MQ=255)
                                    aGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGa                                                                 >  2:2501268/1‑100 (MQ=255)
                                     gCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGAt                                                                >  1:2902017/1‑100 (MQ=255)
                                      cAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATa                                                               <  1:289966/100‑1 (MQ=255)
                                       aaaTGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATaa                                                              <  2:3074849/100‑1 (MQ=255)
                                           gcgcGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGAt                                                          <  2:1340573/100‑1 (MQ=255)
                                                 tGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTc                                                    >  1:2667524/1‑100 (MQ=255)
                                                 tGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTc                                                    <  2:475837/100‑1 (MQ=255)
                                                  gTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCg                                                   <  1:3257598/100‑1 (MQ=255)
                                                    aGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGcc                                                 <  1:970894/100‑1 (MQ=255)
                                                    aGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGcc                                                 >  1:2614579/1‑100 (MQ=255)
                                                    aGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGcc                                                 <  1:777371/100‑1 (MQ=255)
                                                       tgctgACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATCCAGCTGGTTTAGCCCgg                                              >  1:1652179/1‑100 (MQ=255)
                                                        gctgACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGt                                             >  2:2206174/1‑100 (MQ=255)
                                                         ctgACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGtt                                            <  1:2206188/100‑1 (MQ=255)
                                                         ctgACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGtt                                            <  2:1575960/100‑1 (MQ=255)
                                                          tgACCGTGATGATAGTGACATTTTTCTTCTCCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTg                                           <  2:3370185/100‑1 (MQ=255)
                                                            aCCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGaa                                         <  1:2227937/100‑1 (MQ=255)
                                                             ccGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAAc                                        >  2:815252/1‑100 (MQ=255)
                                                             ccGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAAc                                        >  2:1988709/1‑100 (MQ=255)
                                                             ccGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAAc                                        >  2:1186035/1‑100 (MQ=255)
                                                               gTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTGGCCCGGTTg                                           >  2:2245365/1‑95 (MQ=255)
                                                                tgatgaTAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAg                                     >  1:3367902/1‑100 (MQ=255)
                                                                 gatgatAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGc                                    <  1:605730/100‑1 (MQ=255)
                                                                   tgatAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGa                                  >  2:2772961/1‑100 (MQ=255)
                                                                      tAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGAcgc                               <  2:1895850/100‑1 (MQ=255)
                                                                        gTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCgg                             <  2:3070224/100‑1 (MQ=255)
                                                                          gACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCa                           <  2:15449/100‑1 (MQ=255)
                                                                            cATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCaaa                         >  2:483242/1‑100 (MQ=255)
                                                                            cATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCaaa                         >  1:2788210/1‑100 (MQ=255)
                                                                            cATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCaaa                         >  1:2787207/1‑100 (MQ=255)
                                                                             aTTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGTGCATTGCTGCTTCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCTACGCGGCAAAc                        >  2:1516623/1‑100 (MQ=255)
                                                                             aTTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAAc                        <  1:2220068/100‑1 (MQ=255)
                                                                                   gccctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCg                  >  1:2486705/7‑100 (MQ=38)
                                                                                   cttctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCg                  <  2:3787241/100‑1 (MQ=255)
                                                                                    ttctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGa                 <  2:81944/100‑1 (MQ=255)
                                                                                    ttctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGa                 >  1:2951122/1‑100 (MQ=255)
                                                                                    ccctacTCGCCCGGACTCTGGGCGAGCAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGa                 >  1:2331212/6‑100 (MQ=37)
                                                                                     tctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCGGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAg                >  2:1512129/1‑100 (MQ=255)
                                                                                      ctacTCGCCCGGACTCTGGGCGCGTAGGGCATTGCTGCTGCCATTTTCGAGAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAGACTccg                    >  2:2954618/4‑93 (MQ=255)
                                                                                      ctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGt               >  1:1132670/4‑100 (MQ=255)
                                                                                      ctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGt               >  1:1708568/4‑100 (MQ=255)
                                                                                      ctacTCGCCCGGACTCTGGGCGAGCAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGt               >  1:1959111/4‑100 (MQ=38)
                                                                                       tGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGtt              >  1:3422637/1‑100 (MQ=255)
                                                                                         cTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCa            >  2:8051/1‑100 (MQ=255)
                                                                                          tCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCAc           <  1:2388769/100‑1 (MQ=255)
                                                                                          tCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCAc           <  1:1186044/100‑1 (MQ=255)
                                                                                           cGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACg          >  1:249592/1‑100 (MQ=255)
                                                                                            gCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGc         <  1:1294887/100‑1 (MQ=255)
                                                                                             cccAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCt        >  2:1630505/1‑100 (MQ=255)
                                                                                              ccAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTGTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGtt              <  2:1900318/93‑1 (MQ=255)
                                                                                               cAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTc      <  1:958460/100‑1 (MQ=255)
                                                                                                agagTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCa     <  1:387005/100‑1 (MQ=255)
                                                                                                 gagTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAg    <  2:1914034/100‑1 (MQ=255)
                                                                                                  agTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAg    <  1:2641917/99‑1 (MQ=255)
                                                                                                   gTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCg  <  1:1675116/100‑1 (MQ=255)
                                                                                                   gTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCg  <  2:1306458/100‑1 (MQ=255)
                                                                                                   gTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCg  >  1:645805/1‑100 (MQ=255)
                                                                                                   gTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCg  <  2:2963816/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
CCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCG  >  NC_000913/1343937‑1344135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: