Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,445,621 G→C 17.9% W857C (TGG→TGC ydbH → putative membrane‑anchored protein, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,445,6210GC17.9% 259.8 / 48.0 100W857C (TGG→TGCydbHputative membrane‑anchored protein, function unknown
Reads supporting (aligned to +/- strand):  ref base G (32/50);  new base C (6/12);  total (38/62)
Fisher's exact test for biased strand distribution p-value = 7.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 101 total aligned reads displayed.

GATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTT  >  NC_000913/1445523‑1445720
                                                                                                  |                                                                                                   
gATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGc                                                                                                    <  1:3440433/100‑1 (MQ=255)
gATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAAGATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGc                                                                                                    <  1:632610/100‑1 (MQ=255)
  tGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTg                                                                                                  >  2:3543209/1‑100 (MQ=255)
  tGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTAAGCTTTGGCGATAATCTCCAGGCCTGGCTg                                                                                                  >  2:196257/1‑100 (MQ=255)
   ggTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTgg                                                                                                 <  2:1213894/100‑1 (MQ=255)
   ggTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTgg                                                                                                 >  1:2018636/1‑100 (MQ=255)
   ggTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTgg                                                                                                 <  2:3131710/100‑1 (MQ=255)
    gTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGa                                                                                                >  1:2466627/1‑100 (MQ=255)
    gTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGa                                                                                                >  1:3369671/1‑100 (MQ=255)
     tAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAg                                                                                               >  2:3622485/1‑100 (MQ=255)
      aaaaGCGGTAGGGTAAACCGTAATTACCATCATGAAGAGAATATGTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGc                                                                                              <  2:41965/100‑1 (MQ=255)
      aaaaGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGc                                                                                              >  1:1177068/1‑100 (MQ=255)
      aaaaGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGc                                                                                              >  2:2107435/1‑100 (MQ=255)
      aaaaGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGc                                                                                              <  2:2814980/100‑1 (MQ=255)
      aaaaGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGCCATGCCTGGAga                                                                                              <  1:2797129/100‑2 (MQ=255)
      aaaaGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGCCATGCCTGGAga                                                                                              <  1:2142557/100‑2 (MQ=255)
      aaaaGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGCCATGCCTGGAga                                                                                              <  1:447818/100‑2 (MQ=255)
      aaaaGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGCCATGCCTGGAga                                                                                              <  1:2024447/100‑2 (MQ=255)
       aaaGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGCCATGCCTGGAgat                                                                                             <  2:169695/100‑3 (MQ=255)
       aaaGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGCCATGCCTGGAgat                                                                                             <  1:1798774/100‑3 (MQ=255)
        aaGCGGTACGGTAAACCTTGATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGCCATGCCTGGAgat                                                                                             <  1:833215/99‑3 (MQ=255)
        aaGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGCCATGCCTGGAgatt                                                                                            <  2:415914/100‑4 (MQ=255)
         aGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGa                                                                                           >  1:1969996/1‑100 (MQ=255)
           cggtacggtaAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAAc                                                                                         >  1:1525891/1‑100 (MQ=255)
            ggtacggtaAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACg                                                                                        >  1:144773/1‑100 (MQ=255)
            ggtacggtaAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGCCATGCCTGGAgattatcg                                                                                        <  1:1285284/100‑8 (MQ=255)
             gtacggtaAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGc                                                                                       <  1:1638149/100‑1 (MQ=255)
             gtacggtaAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGCCATGCCTGGAgattatcgc                                                                                       <  1:2208195/100‑9 (MQ=38)
             gtacggtaAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGCCATGCCTGGAgattatcgc                                                                                       <  1:259218/100‑9 (MQ=38)
             gtacggtaAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGCCATGCCTGGAgattatcgc                                                                                       <  1:3052116/100‑9 (MQ=39)
              tacggtaAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCa                                                                                      <  1:251113/100‑1 (MQ=255)
              tacggtaAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCa                                                                                      <  1:1272979/100‑1 (MQ=255)
                cggtaAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACg                                                                                    >  2:327655/1‑100 (MQ=255)
                cggtaAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACg                                                                                    <  1:2017928/100‑1 (MQ=255)
                   taAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCt                                                                                 >  1:1585047/1‑100 (MQ=255)
                   taAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCt                                                                                 >  1:739802/1‑100 (MQ=255)
                      aCCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGcc                                                                              <  2:1477950/100‑1 (MQ=255)
                        cTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCgg                                                                            <  1:2106516/100‑1 (MQ=255)
                         ttAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCggg                                                                           <  1:1926097/100‑1 (MQ=255)
                             ttACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAAt                                                                       <  1:498512/100‑1 (MQ=255)
                             ttACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTGTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAAt                                                                       <  2:671385/100‑1 (MQ=255)
                                ccATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGAc                                                                    >  1:935552/1‑100 (MQ=255)
                                ccATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGAc                                                                    <  1:2848276/100‑1 (MQ=255)
                                ccATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGAc                                                                    <  2:3532609/100‑1 (MQ=255)
                                ccATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTGGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGAc                                                                    <  1:749066/100‑1 (MQ=255)
                                  atcatGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTg                                                                  >  1:3713302/1‑100 (MQ=255)
                                  atcatGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTg                                                                  <  1:1044711/100‑1 (MQ=255)
                                  atcatGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTg                                                                  <  1:1382725/100‑1 (MQ=255)
                                       gAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGc                                                             >  2:2792109/1‑100 (MQ=255)
                                         agagAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCaa                                                           <  1:1511087/100‑1 (MQ=255)
                                            gtatatTTTTTCGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCGGCCGGGAAATGACTGTCCGCAAgg                                                         <  2:1923394/97‑1 (MQ=255)
                                                   tttACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGAACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAagag                                                  <  2:341015/99‑1 (MQ=255)
                                                    ttACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAgtg                                                <  1:1114578/100‑1 (MQ=255)
                                                    ttACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAgtg                                                <  2:2015369/100‑1 (MQ=255)
                                                    ttACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAgtg                                                >  2:2526399/1‑100 (MQ=255)
                                                    ttACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAgtg                                                <  1:505276/100‑1 (MQ=255)
                                                     tACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAGGAgtgt                                               <  2:3588687/100‑1 (MQ=255)
                                                      aCGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCCAGGAAAAGAgtgt                                               >  2:1274226/1‑99 (MQ=255)
                                                      aCGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGtgtg                                              <  1:2243648/100‑1 (MQ=255)
                                                      aCGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGtgtg                                              <  2:1635920/100‑1 (MQ=255)
                                                       cGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGa                                             <  1:1755696/100‑1 (MQ=255)
                                                        gCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGa                                             <  1:570832/99‑1 (MQ=255)
                                                         cTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAgg                                           <  1:1499446/100‑1 (MQ=255)
                                                         cTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAgg                                           >  1:194757/1‑100 (MQ=255)
                                                          tgtgGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAgg                                           <  2:1056720/99‑1 (MQ=255)
                                                               cgcAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAAc                                     >  2:2780474/1‑100 (MQ=255)
                                                                  aGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAAt                                  <  1:2984548/100‑1 (MQ=255)
                                                                     ttACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGaa                               >  1:286252/1‑100 (MQ=255)
                                                                      tACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGaaa                              >  2:2711652/1‑100 (MQ=255)
                                                                        cGCTTTGGCGATAATTTCCAGGCATGGCTGGAGCAGAACGAACGTCTGCGGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAAt                            <  2:1787507/100‑1 (MQ=255)
                                                                        cGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAGAAGAGTGTGAGGAAAAACAATGAAAAt                            <  2:3079483/100‑1 (MQ=255)
                                                                        cGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAAt                            <  2:2797116/100‑1 (MQ=255)
                                                                          cTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAAttt                          >  2:3251296/1‑100 (MQ=255)
                                                                           tttGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAAtttt                         <  1:3622512/100‑1 (MQ=255)
                                                                             gtgcgttctgctcCAGCCATGCCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTAc                       >  1:3462209/11‑100 (MQ=38)
                                                                              ggCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACt                      >  1:3647444/1‑100 (MQ=255)
                                                                                 gttctgctcCAGCCATGCCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGc                   >  1:257460/7‑100 (MQ=255)
                                                                                    ctgctcCAGCCATGCCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGc                >  2:445119/4‑100 (MQ=255)
                                                                                    ctgctcCAGCCATGCCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGc                >  2:1214019/4‑100 (MQ=255)
                                                                                     tgctcCAGCCATGCCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCg               >  1:2821614/3‑100 (MQ=255)
                                                                                       ctcGAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGtt             <  2:666418/100‑1 (MQ=255)
                                                                                       ctcCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGtt             <  1:2478024/100‑1 (MQ=255)
                                                                                       ctcCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGtt             >  2:2541115/1‑100 (MQ=255)
                                                                                       ctcCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGtt             <  2:1177059/100‑1 (MQ=255)
                                                                                        tcCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTg            >  1:1155189/1‑100 (MQ=255)
                                                                                        tcCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTg            <  1:3251322/100‑1 (MQ=255)
                                                                                        tcCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTg            >  1:2804514/1‑100 (MQ=255)
                                                                                        tcCAGCCATGCCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTg            >  1:2791012/1‑100 (MQ=255)
                                                                                         ccAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGa           >  2:1985182/1‑100 (MQ=255)
                                                                                          cAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGAc          >  2:3552492/1‑100 (MQ=255)
                                                                                           aGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGTGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACg         <  1:2585851/100‑1 (MQ=255)
                                                                                           aGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGAc          <  1:3783006/99‑1 (MQ=255)
                                                                                           aGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACg         <  1:2657441/100‑1 (MQ=255)
                                                                                           aGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACg         <  1:2792129/100‑1 (MQ=255)
                                                                                           aGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACg         >  1:1807254/1‑100 (MQ=255)
                                                                                           aGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACg         <  2:372454/100‑1 (MQ=255)
                                                                                           aGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACg         <  2:31534/100‑1 (MQ=255)
                                                                                           aGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACg         <  1:1917109/100‑1 (MQ=255)
                                                                                           aGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGAGTAAGAAAAAACAATGAAAATTTTACTGGCTGCGTTGACg         <  2:1428888/100‑1 (MQ=255)
                                                                                                  gCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCtt  >  1:2721246/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
GATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTT  >  NC_000913/1445523‑1445720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: