BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,856,334	C→A	16.1%	E199* (GAG→TAG) ‡	ydjH ←	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,856,334	0	C	A	16.1%	304.3 / 40.8	117	E199* (GAG→TAG)	ydjH	putative kinase
Reads supporting (aligned to +/- strand): ref base C (50/48); new base A (10/9); total (60/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 122 total aligned reads displayed.

CGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAAT  >  NC_000913/1856235‑1856433

cGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCCGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTc                                                                                                     >  2:3348307/1‑100 (MQ=255)

cGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTc                                                                                                     >  2:3711003/1‑100 (MQ=255)

 gTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCg                                                                                                    >  2:2008759/1‑100 (MQ=255)

  tCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCgg                                                                                                   <  2:2108722/100‑1 (MQ=255)

  tCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCgg                                                                                                   <  1:2037130/100‑1 (MQ=255)

  tCTGTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTGCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCgg                                                                                                   <  1:918228/100‑1 (MQ=255)

   cTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGc                                                                                                  <  2:1324155/100‑1 (MQ=255)

     ntACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTc                                                                                                     >  2:631529/2‑95 (MQ=255)

      tACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCaaa                                                                                               <  1:1786792/100‑1 (MQ=255)

       aCCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCaaaa                                                                                              >  1:2391343/1‑100 (MQ=255)

        cccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAAt                                                                                             >  1:2238662/1‑100 (MQ=255)

        cccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAAt                                                                                             <  1:1071380/100‑1 (MQ=255)

        cccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAAt                                                                                             >  1:1772821/1‑100 (MQ=255)

        cccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAAt                                                                                             >  2:3136158/1‑100 (MQ=255)

         ccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAAtt                                                                                            >  1:3052367/1‑100 (MQ=255)

          cGTTTTAATCACCACCGTTTTTTCGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCATTGACTCTTGCCCGGTGAGTAATTTTGCCTCGGCAAAATTa                                                                                           <  2:1116927/100‑1 (MQ=255)

          cGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTa                                                                                           >  2:124936/1‑100 (MQ=255)

           gTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCGTAATTAg                                                                                          >  1:2367336/1‑100 (MQ=255)

           gTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAg                                                                                          >  1:2446307/1‑100 (MQ=255)

           gTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAg                                                                                          >  2:1091437/1‑100 (MQ=255)

           gTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTac                                                                                          <  2:2778118/100‑2 (MQ=255)

           gTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTac                                                                                          <  2:2783468/100‑2 (MQ=255)

           gTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTac                                                                                          <  2:557665/100‑2 (MQ=255)

            ttttAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAgg                                                                                         <  2:3305206/100‑1 (MQ=255)

            ttttAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAgg                                                                                         <  1:1987874/100‑1 (MQ=255)

             tttAATCACCACCGTTTTTACGTCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTactc                                                                                        <  2:3049666/100‑4 (MQ=255)

             tttAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGa                                                                                        >  1:1533474/1‑100 (MQ=255)

             tttAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTactc                                                                                        <  2:1302211/100‑4 (MQ=255)

             tttAATCACCACCGTTTTTACGCCGAACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTGCCGGTGAGTAATTTTGCCGAGGCAAAATTacn                                                                                         <  2:3741386/99‑3 (MQ=255)

              ttAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTactca                                                                                       <  2:3409459/100‑5 (MQ=39)

                 aTCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACa                                                                                    >  2:948529/1‑100 (MQ=255)

                 aTCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTactcaccg                                                                                    <  1:3061798/100‑8 (MQ=38)

                  tCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAg                                                                                   >  1:3528965/1‑100 (MQ=255)

                  tCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAg                                                                                   >  1:2136391/1‑100 (MQ=255)

                   caccacCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTTATCCAGTGTTGCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGa                                                                                  >  2:2615043/1‑100 (MQ=255)

                     ccaccaTTTTTACGTCGCACTCAAGAAAGCAGTCACCACTTTCAGCCAGTGTCCCTCCCCCGGTGAGTAATTTTGCCGAGGCAAAATTactcaccggg                                                                                  <  2:552876/98‑10 (MQ=16)

                     ccaccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATa                                                                                >  2:1760100/1‑100 (MQ=255)

                     ccaccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATa                                                                                >  1:2192282/1‑100 (MQ=255)

                       accGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAAt                                                                              <  2:2470462/100‑1 (MQ=255)

                         cGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCg                                                                            <  1:2056988/100‑1 (MQ=255)

                         cGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCg                                                                            <  1:422204/100‑1 (MQ=255)

                          gTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCTGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGa                                                                           <  2:2672494/100‑1 (MQ=255)

                           tttttACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGAc                                                                          <  2:1351468/100‑1 (MQ=255)

                           tttttACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGAc                                                                          <  1:1322880/100‑1 (MQ=255)

                            ttttACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGCTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGa                                                                           <  2:1156763/98‑1 (MQ=255)

                             tttACGCCGCACGCAAGAAAGCAGTCAGCAATTTCAGCCAGGGTCTCTCTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACa                                                                         <  1:3117293/99‑1 (MQ=255)

                              ttACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATa                                                                       >  2:815321/1‑100 (MQ=255)

                                aCGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAAc                                                                     >  2:3768693/1‑100 (MQ=255)

                                 cgccgcACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACt                                                                    >  1:678909/1‑100 (MQ=255)

                                    cgcacgcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCaa                                                                 <  1:2390246/100‑1 (MQ=255)

                                    cgcacgcaAGAAAGCAGTCAGCAATTTCATCCAGTCTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCaa                                                                 <  2:2155123/100‑1 (MQ=255)

                                     gcacgcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAAt                                                                <  2:296594/100‑1 (MQ=255)

                                      cacgcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACACAACTCAATg                                                               >  1:1303272/1‑100 (MQ=255)

                                          caAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTc                                                           <  1:268883/100‑1 (MQ=255)

                                          caAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTc                                                           <  1:1196655/100‑1 (MQ=255)

                                          caAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTc                                                           >  2:1456802/1‑100 (MQ=255)

                                            aGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGc                                                         <  1:2840896/100‑1 (MQ=255)

                                            aGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGc                                                         <  1:725105/100‑1 (MQ=255)

                                             gAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCa                                                        >  2:2823090/1‑100 (MQ=255)

                                             gAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCa                                                        >  1:2447597/1‑100 (MQ=255)

                                                 gCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAata                                                    <  2:1224800/100‑1 (MQ=255)

                                                 gCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAata                                                    <  2:976691/100‑1 (MQ=255)

                                                 gCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAata                                                    <  1:1593129/100‑1 (MQ=255)

                                                 gCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAata                                                    <  1:3151107/100‑1 (MQ=255)

                                                    gTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATatc                                                  >  2:1624019/1‑99 (MQ=255)

                                                     tCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATatcat                                                <  1:162204/100‑1 (MQ=255)

                                                       aGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATcc                                              >  2:2864284/1‑100 (MQ=255)

                                                           aTTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCg                                          >  1:1654739/1‑100 (MQ=255)

                                                              tCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGttt                                       <  2:2366252/100‑1 (MQ=255)

                                                               cATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGttt                                       <  2:20702/99‑1 (MQ=255)

                                                                 tCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttcat                                    >  2:590960/1‑100 (MQ=255)

                                                                   cAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttcattc                                  >  1:1690549/1‑100 (MQ=255)

                                                                   cAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttcattc                                  <  2:3006972/100‑1 (MQ=255)

                                                                    aGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttaattca                                 >  2:1281408/1‑100 (MQ=255)

                                                                       gtCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAAcc                              >  2:2625898/1‑100 (MQ=255)

                                                                           ctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCgg                          >  2:1949517/1‑100 (MQ=255)

                                                                           ctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCgg                          <  2:1201728/100‑1 (MQ=255)

                                                                                ccGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGa                     >  2:1925066/1‑100 (MQ=255)

                                                                                ccGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGa                     >  1:3671499/1‑100 (MQ=255)

                                                                                 cGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGAt                    <  1:2052519/100‑1 (MQ=255)

                                                                                 cGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGAt                    <  1:2060367/100‑1 (MQ=255)

                                                                                  ggTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATc                   >  2:859430/1‑100 (MQ=255)

                                                                                   tttcctAATTTTGGCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCa                  >  1:2591023/6‑100 (MQ=255)

                                                                                    ttcctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCat                 >  2:1630069/5‑100 (MQ=39)

                                                                                    tGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCat                 <  1:1159470/100‑1 (MQ=255)

                                                                                     gAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCata                >  2:2521955/1‑100 (MQ=255)

                                                                                     gAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCata                <  2:3313962/100‑1 (MQ=255)

                                                                                      cctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCatat               >  1:48217/3‑100 (MQ=255)

                                                                                      cctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCatat               >  1:2108620/3‑100 (MQ=255)

                                                                                      aGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCa                  <  2:2379530/97‑1 (MQ=255)

                                                                                       ctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATc              >  1:2177928/2‑100 (MQ=255)

                                                                                       ctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATc              >  1:2779618/2‑100 (MQ=255)

                                                                                        tAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCg             >  1:1946938/1‑100 (MQ=255)

                                                                                             ttGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGcaca        <  1:433325/100‑1 (MQ=255)

                                                                                               gCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGa      >  2:1952822/1‑100 (MQ=255)

                                                                                                ccTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCCTCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGAt     >  2:3233859/1‑100 (MQ=255)

                                                                                                 cTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATa    <  1:918034/100‑1 (MQ=255)

                                                                                                 cTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATa    <  2:492392/100‑1 (MQ=255)

                                                                                                   cGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAat  >  2:373697/1‑100 (MQ=255)

                                                                                                   cGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAat  >  2:1173421/1‑100 (MQ=255)

CGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAAT  >  NC_000913/1856235‑1856433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`